Details of the Target

General Information of Target

Target ID LDTP16056
Target Name Mid1-interacting protein 1 (MID1IP1)
Gene Name MID1IP1
Gene ID 58526
Synonyms
MIG12; Mid1-interacting protein 1; Gastrulation-specific G12-like protein; Mid1-interacting G12-like protein; Protein STRAIT11499; Spot 14-related protein; S14R; Spot 14-R
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAAARCWRPLLRGPRLSLHTAANAAATATETTCQDVAATPVARYPPIVASMTADSKAARL
RRIERWQATVHAAESVDEKLRILTKMQFMKYMVYPQTFALNADRWYQYFTKTVFLSGLPP
PPAEPEPEPEPEPEPALDLAALRAVACDCLLQEHFYLRRRRRVHRYEESEVISLPFLDQL
VSTLVGLLSPHNPALAAAALDYRCPVHFYWVRGEEIIPRGHRRGRIDDLRYQIDDKPNNQ
IRISKQLAEFVPLDYSVPIEIPTIKCKPDKLPLFKRQYENHIFVGSKTADPCCYGHTQFH
LLPDKLRRERLLRQNCADQIEVVFRANAIASLFAWTGAQAMYQGFWSEADVTRPFVSQAV
ITDGKYFSFFCYQLNTLALTTQADQNNPRKNICWGTQSKPLYETIEDNDVKGFNDDVLLQ
IVHFLLNRPKEEKSQLLEN
Target Bioclass
Other
Family
SPOT14 family
Subcellular location
Nucleus
Function
Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules.
Uniprot ID
Q9NPA3
Ensemble ID
ENST00000336949.7
HGNC ID
HGNC:20715

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K160(10.00); K164(6.05)  LDD0277  [1]
m-APA
 Probe Info  		14.33  LDD0403  [2]
DBIA
 Probe Info  		C62(5.77)  LDD0209  [3]
IA-alkyne
 Probe Info  		C62(0.45)  LDD2183  [4]
IPM
 Probe Info  		N.A.  LDD0025  [5]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [5]
TFBX
 Probe Info  		C62(0.00); C5(0.00)  LDD0027  [5]
VSF
 Probe Info  		N.A.  LDD0007  [6]
AOyne
 Probe Info  		15.00  LDD0443  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 HCT 116 C62(0.98)  LDD0533  [9]
 LDCM0217  AC101 HCT 116 C62(0.82)  LDD0534  [9]
 LDCM0218  AC102 HCT 116 C62(1.03)  LDD0535  [9]
 LDCM0219  AC103 HCT 116 C62(1.21)  LDD0536  [9]
 LDCM0220  AC104 HCT 116 C62(1.11)  LDD0537  [9]
 LDCM0221  AC105 HCT 116 C62(1.02)  LDD0538  [9]
 LDCM0222  AC106 HCT 116 C62(1.20)  LDD0539  [9]
 LDCM0223  AC107 HCT 116 C62(1.36)  LDD0540  [9]
 LDCM0224  AC108 HCT 116 C62(0.91)  LDD0541  [9]
 LDCM0225  AC109 HCT 116 C62(1.00)  LDD0542  [9]
 LDCM0227  AC110 HCT 116 C62(0.75)  LDD0544  [9]
 LDCM0228  AC111 HCT 116 C62(0.79)  LDD0545  [9]
 LDCM0229  AC112 HCT 116 C62(1.09)  LDD0546  [9]
 LDCM0230  AC113 HCT 116 C62(0.92)  LDD0547  [9]
 LDCM0231  AC114 HCT 116 C62(1.23)  LDD0548  [9]
 LDCM0232  AC115 HCT 116 C62(1.51)  LDD0549  [9]
 LDCM0233  AC116 HCT 116 C62(1.52)  LDD0550  [9]
 LDCM0234  AC117 HCT 116 C62(1.19)  LDD0551  [9]
 LDCM0235  AC118 HCT 116 C62(1.15)  LDD0552  [9]
 LDCM0236  AC119 HCT 116 C62(1.20)  LDD0553  [9]
 LDCM0238  AC120 HCT 116 C62(1.18)  LDD0555  [9]
 LDCM0239  AC121 HCT 116 C62(1.14)  LDD0556  [9]
 LDCM0240  AC122 HCT 116 C62(1.32)  LDD0557  [9]
 LDCM0241  AC123 HCT 116 C62(1.29)  LDD0558  [9]
 LDCM0242  AC124 HCT 116 C62(1.19)  LDD0559  [9]
 LDCM0243  AC125 HCT 116 C62(1.12)  LDD0560  [9]
 LDCM0244  AC126 HCT 116 C62(1.32)  LDD0561  [9]
 LDCM0245  AC127 HCT 116 C62(1.33)  LDD0562  [9]
 LDCM0263  AC143 HCT 116 C62(1.18)  LDD0580  [9]
 LDCM0264  AC144 HCT 116 C62(1.77)  LDD0581  [9]
 LDCM0265  AC145 HCT 116 C62(1.28)  LDD0582  [9]
 LDCM0266  AC146 HCT 116 C62(1.77)  LDD0583  [9]
 LDCM0267  AC147 HCT 116 C62(1.74)  LDD0584  [9]
 LDCM0268  AC148 HCT 116 C62(2.44)  LDD0585  [9]
 LDCM0269  AC149 HCT 116 C62(1.71)  LDD0586  [9]
 LDCM0271  AC150 HCT 116 C62(1.12)  LDD0588  [9]
 LDCM0272  AC151 HCT 116 C62(1.49)  LDD0589  [9]
 LDCM0273  AC152 HCT 116 C62(1.75)  LDD0590  [9]
 LDCM0274  AC153 HCT 116 C62(2.84)  LDD0591  [9]
 LDCM0621  AC154 HCT 116 C62(1.39)  LDD2158  [9]
 LDCM0622  AC155 HCT 116 C62(1.71)  LDD2159  [9]
 LDCM0623  AC156 HCT 116 C62(1.30)  LDD2160  [9]
 LDCM0624  AC157 HCT 116 C62(1.19)  LDD2161  [9]
 LDCM0320  AC57 HCT 116 C62(1.21)  LDD0637  [9]
 LDCM0321  AC58 HCT 116 C62(1.26)  LDD0638  [9]
 LDCM0322  AC59 HCT 116 C62(1.27)  LDD0639  [9]
 LDCM0324  AC60 HCT 116 C62(1.28)  LDD0641  [9]
 LDCM0325  AC61 HCT 116 C62(1.38)  LDD0642  [9]
 LDCM0326  AC62 HCT 116 C62(1.63)  LDD0643  [9]
 LDCM0327  AC63 HCT 116 C62(1.34)  LDD0644  [9]
 LDCM0328  AC64 HCT 116 C62(1.74)  LDD0645  [9]
 LDCM0329  AC65 HCT 116 C62(1.36)  LDD0646  [9]
 LDCM0330  AC66 HCT 116 C62(1.51)  LDD0647  [9]
 LDCM0331  AC67 HCT 116 C62(1.55)  LDD0648  [9]
 LDCM0349  AC83 HCT 116 C62(1.65)  LDD0666  [9]
 LDCM0350  AC84 HCT 116 C62(1.39)  LDD0667  [9]
 LDCM0351  AC85 HCT 116 C62(1.19)  LDD0668  [9]
 LDCM0352  AC86 HCT 116 C62(1.30)  LDD0669  [9]
 LDCM0353  AC87 HCT 116 C62(0.95)  LDD0670  [9]
 LDCM0354  AC88 HCT 116 C62(1.11)  LDD0671  [9]
 LDCM0355  AC89 HCT 116 C62(1.30)  LDD0672  [9]
 LDCM0357  AC90 HCT 116 C62(0.93)  LDD0674  [9]
 LDCM0358  AC91 HCT 116 C62(1.46)  LDD0675  [9]
 LDCM0359  AC92 HCT 116 C62(1.36)  LDD0676  [9]
 LDCM0360  AC93 HCT 116 C62(1.11)  LDD0677  [9]
 LDCM0361  AC94 HCT 116 C62(1.23)  LDD0678  [9]
 LDCM0362  AC95 HCT 116 C62(1.26)  LDD0679  [9]
 LDCM0363  AC96 HCT 116 C62(1.26)  LDD0680  [9]
 LDCM0364  AC97 HCT 116 C62(1.41)  LDD0681  [9]
 LDCM0365  AC98 HCT 116 C62(1.86)  LDD0682  [9]
 LDCM0366  AC99 HCT 116 C62(0.92)  LDD0683  [9]
 LDCM0156  Aniline NCI-H1299 14.33  LDD0403  [2]
 LDCM0632  CL-Sc Hep-G2 C5(1.78); C5(0.49)  LDD2227  [8]
 LDCM0396  CL125 HCT 116 C62(0.91)  LDD0713  [9]
 LDCM0397  CL126 HCT 116 C62(1.14)  LDD0714  [9]
 LDCM0398  CL127 HCT 116 C62(1.14)  LDD0715  [9]
 LDCM0399  CL128 HCT 116 C62(1.76)  LDD0716  [9]
 LDCM0420  CL31 HCT 116 C62(0.84)  LDD0737  [9]
 LDCM0421  CL32 HCT 116 C62(0.77)  LDD0738  [9]
 LDCM0422  CL33 HCT 116 C62(0.97)  LDD0739  [9]
 LDCM0423  CL34 HCT 116 C62(0.96)  LDD0740  [9]
 LDCM0424  CL35 HCT 116 C62(0.86)  LDD0741  [9]
 LDCM0425  CL36 HCT 116 C62(0.90)  LDD0742  [9]
 LDCM0426  CL37 HCT 116 C62(0.94)  LDD0743  [9]
 LDCM0428  CL39 HCT 116 C62(0.76)  LDD0745  [9]
 LDCM0430  CL40 HCT 116 C62(1.13)  LDD0747  [9]
 LDCM0431  CL41 HCT 116 C62(0.90)  LDD0748  [9]
 LDCM0432  CL42 HCT 116 C62(1.08)  LDD0749  [9]
 LDCM0433  CL43 HCT 116 C62(0.95)  LDD0750  [9]
 LDCM0434  CL44 HCT 116 C62(1.03)  LDD0751  [9]
 LDCM0435  CL45 HCT 116 C62(1.04)  LDD0752  [9]
 LDCM0634  CY-0357 Hep-G2 C5(0.31)  LDD2228  [8]
 LDCM0625  F8 Ramos C62(0.79)  LDD2187  [4]
 LDCM0572  Fragment10 Ramos C62(0.98)  LDD2189  [4]
 LDCM0574  Fragment12 Ramos C62(1.31)  LDD2191  [4]
 LDCM0575  Fragment13 Ramos C62(1.43)  LDD2192  [4]
 LDCM0579  Fragment20 Ramos C62(1.40)  LDD2194  [4]
 LDCM0580  Fragment21 Ramos C62(0.89)  LDD2195  [4]
 LDCM0582  Fragment23 Ramos C62(0.74)  LDD2196  [4]
 LDCM0578  Fragment27 Ramos C62(1.32)  LDD2197  [4]
 LDCM0586  Fragment28 Ramos C62(0.91)  LDD2198  [4]
 LDCM0588  Fragment30 Ramos C62(1.18)  LDD2199  [4]
 LDCM0589  Fragment31 Ramos C62(1.58)  LDD2200  [4]
 LDCM0468  Fragment33 Ramos C62(0.97)  LDD2202  [4]
 LDCM0596  Fragment38 Ramos C62(0.89)  LDD2203  [4]
 LDCM0566  Fragment4 Ramos C62(1.77)  LDD2184  [4]
 LDCM0427  Fragment51 HCT 116 C62(1.54)  LDD0744  [9]
 LDCM0610  Fragment52 Ramos C62(1.22)  LDD2204  [4]
 LDCM0614  Fragment56 Ramos C62(0.62)  LDD2205  [4]
 LDCM0571  Fragment9 Ramos C62(1.29)  LDD2188  [4]
 LDCM0023  KB03 Jurkat C62(5.77)  LDD0209  [3]
 LDCM0627  NUDT7-COV-1 HEK-293T C5(0.79)  LDD2206  [10]
 LDCM0628  OTUB2-COV-1 HEK-293T C5(0.86)  LDD2207  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Adenylate kinase 8 (AK8) Adenylate kinase family Q96MA6
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Thyrotropin receptor (TSHR) G-protein coupled receptor 1 family P16473

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
3 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
7 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
10 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.