Details of the Target

General Information of Target

Target ID LDTP14042
Target Name Malignant fibrous histiocytoma-amplified sequence 1 (MFHAS1)
Gene Name MFHAS1
Gene ID 9258
Synonyms
MASL1; Malignant fibrous histiocytoma-amplified sequence 1; Malignant fibrous histiocytoma-amplified sequence with leucine-rich tandem repeats 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MATPSAAFEALMNGVTSWDVPEDAVPCELLLIGEASFPVMVNDMGQVLIAASSYGRGRLV
VVSHEDYLVEAQLTPFLLNAVGWLCSSPGAPIGVHPSLAPLAKILEGSGVDAKVEPEVKD
SLGVYCIDAYNETMTEKLVKFMKCGGGLLIGGQAWDWANQGEDERVLFTFPGNLVTSVAG
IYFTDNKGDTSFFKVSKKMPKIPVLVSCEDDLSDDREELLHGISELDISNSDCFPSQLLV
HGALAFPLGLDSYHGCVIAAARYGRGRVVVTGHKVLFTVGKLGPFLLNAVRWLDGGRRGK
VVVQTELRTLSGLLAVGGIDTSIEPNLTSDASVYCFEPVSEVGVKELQEFVAEGGGLFVG
AQAWWWAFKNPGVSPLARFPGNLLLNPFGISITSQSLNPGPFRTPKAGIRTYHFRSTLAE
FQVIMGRKRGNVEKGWLAKLGPDGAAFLQIPAEEIPAYMSVHRLLRKLLSRYRLPVATRE
NPVINDCCRGAMLSLATGLAHSGSDLSLLVPEIEDMYSSPYLRPSESPITVEVNCTNPGT
RYCWMSTGLYIPGRQIIEVSLPEAAASADLKIQIGCHTDDLTRASKLFRGPLVINRCCLD
KPTKSITCLWGGLLYIIVPQNSKLGSVPVTVKGAVHAPYYKLGETTLEEWKRRIQENPGP
WGELATDNIILTVPTANLRTLENPEPLLRLWDEVMQAVARLGAEPFPLRLPQRIVADVQI
SVGWMHAGYPIMCHLESVQELINEKLIRTKGLWGPVHELGRNQQRQEWEFPPHTTEATCN
LWCVYVHETVLGIPRSRANIALWPPVREKRVRIYLSKGPNVKNWNAWTALETYLQLQEAF
GWEPFIRLFTEYRNQTNLPTENVDKMNLWVKMFSHQVQKNLAPFFEAWAWPIQKEVATSL
AYLPEWKENIMKLYLLTQMPH
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Probable GTP-binding protein. Functions in innate immunity and more specifically the inflammatory response as a regulator of the Toll-like receptor TLR2 and TLR4 signaling pathways. Negatively regulates the part of the TLR4 signaling pathway that leads to the activation of the transcription factor AP-1. By retaining the phosphatase complex PP2A into the cytoplasm, prevents the dephosphorylation of the AP-1 subunit JUN which is required for proper activation of the transcription factor. Both inhibits and activates the TLR2-dependent signaling pathway. Positively regulates the TLR2 signaling pathway to activate specifically the downstream p38 and JNK MAP kinases and promote the polarization of macrophages toward the pro-inflammatory M1 phenotype. It may also play a role in the regulation of inflammation induced by high glucose through the PKB/AKT signaling pathway. Also involved in erythrocyte differentiation through activation of the ERK1/ERK2 signaling pathway.
Uniprot ID
Q9Y4C4
Ensemble ID
ENST00000276282.7
HGNC ID
HGNC:16982

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
NAIA_5
 Probe Info  		C1040(20.00)  LDD2227  [1]
DBIA
 Probe Info  		C629(1.12)  LDD0531  [2]
CY-1
 Probe Info  		N.A.  LDD0246  [3]
IA-alkyne
 Probe Info  		C437(0.00); C629(0.00); C446(0.00)  LDD0167  [4]
Acrolein
 Probe Info  		N.A.  LDD0217  [5]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C629(1.12)  LDD0531  [2]
 LDCM0226  AC11 HEK-293T C428(0.90)  LDD1509  [6]
 LDCM0263  AC143 HCT 116 C437(1.03)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C437(0.98)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C437(1.03)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C437(1.12)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C437(1.32)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C437(0.93)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C437(0.92)  LDD0586  [2]
 LDCM0271  AC150 HCT 116 C437(1.01)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C437(1.01)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C437(0.95)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C437(0.99)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C437(0.95)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C437(0.94)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C437(0.95)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C437(1.06)  LDD2161  [2]
 LDCM0276  AC17 HCT 116 C437(0.95)  LDD0593  [2]
 LDCM0277  AC18 HCT 116 C437(0.94)  LDD0594  [2]
 LDCM0278  AC19 HCT 116 C437(0.84)  LDD0595  [2]
 LDCM0279  AC2 HCT 116 C629(1.28)  LDD0596  [2]
 LDCM0280  AC20 HCT 116 C437(0.98)  LDD0597  [2]
 LDCM0281  AC21 HCT 116 C437(0.95)  LDD0598  [2]
 LDCM0282  AC22 HCT 116 C437(0.83)  LDD0599  [2]
 LDCM0283  AC23 HCT 116 C437(0.80)  LDD0600  [2]
 LDCM0284  AC24 HCT 116 C437(0.88)  LDD0601  [2]
 LDCM0287  AC27 HEK-293T C428(0.93)  LDD1526  [6]
 LDCM0290  AC3 HCT 116 C629(1.31)  LDD0607  [2]
 LDCM0293  AC32 HEK-293T C629(0.91)  LDD1532  [6]
 LDCM0296  AC35 HCT 116 C629(0.91); C437(1.21)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C437(0.85); C629(0.90)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C629(0.92); C437(1.41)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C437(0.91); C629(1.03)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C629(1.05); C437(1.46)  LDD0617  [2]
 LDCM0301  AC4 HCT 116 C629(1.13)  LDD0618  [2]
 LDCM0302  AC40 HCT 116 C629(1.04); C437(1.74)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C437(0.79); C629(0.86)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C629(1.04); C437(1.11)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C629(1.05); C437(1.13)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C437(0.95); C629(1.20)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C629(1.07); C437(1.08)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C629(1.01)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C629(0.96)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C629(1.17)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C629(1.23)  LDD0628  [2]
 LDCM0312  AC5 HCT 116 C629(1.14)  LDD0629  [2]
 LDCM0313  AC50 HCT 116 C629(1.21)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C629(1.11)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C629(1.11)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C629(1.01)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C629(1.11)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C629(0.87)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C629(1.12)  LDD0636  [2]
 LDCM0320  AC57 HCT 116 C629(1.10)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C629(1.31)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C629(1.96)  LDD0639  [2]
 LDCM0324  AC60 HCT 116 C629(1.22)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C629(1.13)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C629(1.60)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C629(1.73)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C629(1.33)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C629(1.45)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C629(1.36)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C629(0.97)  LDD0648  [2]
 LDCM0345  AC8 HEK-293T C629(0.90)  LDD1569  [6]
 LDCM0275  AKOS034007705 HEK-293T C629(1.00)  LDD1514  [6]
 LDCM0632  CL-Sc Hep-G2 C1040(20.00)  LDD2227  [1]
 LDCM0367  CL1 HEK-293T C629(1.06)  LDD1571  [6]
 LDCM0369  CL100 HCT 116 C629(1.17)  LDD0686  [2]
 LDCM0370  CL101 HEK-293T C629(0.93)  LDD1574  [6]
 LDCM0371  CL102 HEK-293T C629(0.86)  LDD1575  [6]
 LDCM0374  CL105 HCT 116 C437(1.01)  LDD0691  [2]
 LDCM0375  CL106 HCT 116 C437(0.96)  LDD0692  [2]
 LDCM0376  CL107 HCT 116 C437(0.80)  LDD0693  [2]
 LDCM0377  CL108 HCT 116 C437(0.94)  LDD0694  [2]
 LDCM0378  CL109 HCT 116 C437(0.90)  LDD0695  [2]
 LDCM0380  CL110 HCT 116 C437(0.99)  LDD0697  [2]
 LDCM0381  CL111 HCT 116 C437(0.89)  LDD0698  [2]
 LDCM0383  CL113 HEK-293T C629(0.97)  LDD1587  [6]
 LDCM0384  CL114 HEK-293T C629(1.15)  LDD1588  [6]
 LDCM0387  CL117 HCT 116 C629(0.96); C437(1.42)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C629(0.85); C437(1.05)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C629(1.05); C437(1.17)  LDD0706  [2]
 LDCM0390  CL12 HEK-293T C629(1.11)  LDD1594  [6]
 LDCM0391  CL120 HCT 116 C437(1.17); C629(1.24)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C629(0.99)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C629(1.10)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C629(1.00)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C629(1.09)  LDD0712  [2]
 LDCM0396  CL125 HCT 116 C629(1.27)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C629(0.64)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C629(0.97)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C629(1.78)  LDD0716  [2]
 LDCM0400  CL13 HEK-293T C629(1.40)  LDD1604  [6]
 LDCM0401  CL14 HEK-293T C629(1.51)  LDD1605  [6]
 LDCM0403  CL16 HCT 116 C629(1.12)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C629(1.07)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C629(1.23)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C629(1.22)  LDD0723  [2]
 LDCM0407  CL2 HEK-293T C629(0.80)  LDD1611  [6]
 LDCM0408  CL20 HCT 116 C629(1.50)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C629(1.31)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C629(1.70)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C629(1.53)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C629(1.71)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C629(1.44)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C629(1.44)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C629(1.11)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C629(1.28)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C629(1.25)  LDD0734  [2]
 LDCM0419  CL30 HCT 116 C629(1.19)  LDD0736  [2]
 LDCM0420  CL31 HCT 116 C437(1.13)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C437(1.10)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C437(1.19)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C437(1.12)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C437(1.17)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C437(1.37)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C437(1.19)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C437(1.11)  LDD0745  [2]
 LDCM0430  CL40 HCT 116 C437(1.28)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C437(1.09)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C437(1.04)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C437(1.14)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C437(1.24)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C437(1.17)  LDD0752  [2]
 LDCM0438  CL48 HEK-293T C629(1.25)  LDD1642  [6]
 LDCM0439  CL49 HEK-293T C629(1.20)  LDD1643  [6]
 LDCM0441  CL50 HEK-293T C629(0.94)  LDD1645  [6]
 LDCM0446  CL55 HEK-293T C428(0.87)  LDD1649  [6]
 LDCM0452  CL60 HEK-293T C629(1.11)  LDD1655  [6]
 LDCM0453  CL61 HCT 116 C629(1.37)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C629(1.46)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C629(1.20)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C629(1.40)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C629(1.34)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C629(1.57)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C629(1.47)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C629(1.20)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C629(1.60)  LDD0778  [2]
 LDCM0462  CL7 HEK-293T C428(1.02)  LDD1665  [6]
 LDCM0463  CL70 HCT 116 C629(1.07)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C629(1.15)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C629(1.26)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C629(1.08)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C629(1.09)  LDD0784  [2]
 LDCM0472  CL79 HEK-293T C428(0.73)  LDD1675  [6]
 LDCM0478  CL84 HEK-293T C629(1.15)  LDD1681  [6]
 LDCM0479  CL85 HEK-293T C629(1.06)  LDD1682  [6]
 LDCM0480  CL86 HEK-293T C629(0.89)  LDD1683  [6]
 LDCM0486  CL91 HCT 116 C629(1.07)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C629(1.04)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C629(1.16)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C629(1.17)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C629(1.06)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C629(1.27)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C629(1.23)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C629(0.97)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C629(1.07)  LDD0811  [2]
 LDCM0202  EV-93 T cell C1040(4.65)  LDD0514  [7]
 LDCM0468  Fragment33 HCT 116 C629(1.39)  LDD0785  [2]
 LDCM0427  Fragment51 HCT 116 C437(1.71)  LDD0744  [2]
 LDCM0022  KB02 T cell C1040(16.37)  LDD1703  [7]
 LDCM0023  KB03 22RV1 C428(3.63)  LDD2660  [8]
 LDCM0024  KB05 MSTO-211H C428(2.98)  LDD3337  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
60 kDa heat shock protein, mitochondrial (HSPD1) Chaperonin (HSP60) family P10809

References

1 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
8 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840