Details of the Target

General Information of Target

Target ID LDTP12964
Target Name Forkhead box protein J2 (FOXJ2)
Gene Name FOXJ2
Gene ID 55810
Synonyms
FHX; Forkhead box protein J2; Fork head homologous X
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MKSLKAKFRKSDTNEWNKNDDRLLQAVENGDAEKVASLLGKKGASATKHDSEGKTAFHLA
AAKGHVECLRVMITHGVDVTAQDTTGHSALHLAAKNSHHECIRKLLQSKCPAESVDSSGK
TALHYAAAQGCLQAVQILCEHKSPINLKDLDGNIPLLLAVQNGHSEICHFLLDHGADVNS
RNKSGRTALMLACEIGSSNAVEALIKKGADLNLVDSLGYNALHYSKLSENAGIQSLLLSK
ISQDADLKTPTKPKQHDQVSKISSERSGTPKKRKAPPPPISPTQLSDVSSPRSITSTPLS
GKESVFFAEPPFKAEISSIRENKDRLSDSTTGADSLLDISSEADQQDLLSLLQAKVASLT
LHNKELQDKLQAKSPKEAEADLSFDSYHSTQTDLGPSLGKPGETSPPDSKSSPSVLIHSL
GKSTTDNDVRIQQLQEILQDLQKRLESSEAERKQLQVELQSRRAELVCLNNTEISENSSD
LSQKLKETQSKYEEAMKEVLSVQKQMKLGLVSPESMDNYSHFHELRVTEEEINVLKQDLQ
NALEESERNKEKVRELEEKLVEREKGTVIKPPVEEYEEMKSSYCSVIENMNKEKAFLFEK
YQEAQEEIMKLKDTLKSQMTQEASDEAEDMKEAMNRMIDELNKQVSELSQLYKEAQAELE
DYRKRKSLEDVTAEYIHKAEHEKLMQLTNVSRAKAEDALSEMKSQYSKVLNELTQLKQLV
DAQKENSVSITEHLQVITTLRTAAKEMEEKISNLKEHLASKEVEVAKLEKQLLEEKAAMT
DAMVPRSSYEKLQSSLESEVSVLASKLKESVKEKEKVHSEVVQIRSEVSQVKREKENIQT
LLKSKEQEVNELLQKFQQAQEELAEMKRYAESSSKLEEDKDKKINEMSKEVTKLKEALNS
LSQLSYSTSSSKRQSQQLEALQQQVKQLQNQLAECKKQHQEVISVYRMHLLYAVQGQMDE
DVQKVLKQILTMCKNQSQKK
Target Type
Literature-reported
Target Bioclass
Transcription factor
Subcellular location
Nucleus
Function
[Isoform FOXJ2.L]: Transcriptional activator. Able to bind to two different type of DNA binding sites. More effective than isoform FOXJ2.S in transcriptional activation. Plays an important role in spermatogenesis, especially in spermatocyte meiosis.; [Isoform FOXJ2.S]: Transcriptional activator.
TTD ID
T96236
Uniprot ID
Q9P0K8
DrugMap ID
TTV1FDA
Ensemble ID
ENST00000162391.8
HGNC ID
HGNC:24818

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT15 SNV: p.G399C .
HT SNV: p.A220T .
HT115 SNV: p.D143G .
Ishikawa (Heraklio) 02 ER SNV: p.Q379R .
JURKAT SNV: p.A387T Compound 10    Probe Info 
LS123 SNV: p.D342G .
LS180 SNV: p.Q162E .
MCC26 SNV: p.D133N .
NCIH1155 SNV: p.Q192Ter .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
DBIA
 Probe Info  		C145(1.07)  LDD0078  [2]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
NAIA_5
 Probe Info  		N.A.  LDD2225  [4]
Compound 10
 Probe Info  		N.A.  LDD2216  [5]
IPM
 Probe Info  		N.A.  LDD0147  [6]
VSF
 Probe Info  		N.A.  LDD0007  [7]
AOyne
 Probe Info  		15.00  LDD0443  [8]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DA-2
 Probe Info  		N.A.  LDD0071  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C145(1.25)  LDD0531  [2]
 LDCM0215  AC10 HEK-293T C42(1.07)  LDD1508  [10]
 LDCM0237  AC12 HEK-293T C42(1.42)  LDD1510  [10]
 LDCM0270  AC15 HEK-293T C42(1.11)  LDD1513  [10]
 LDCM0276  AC17 HCT 116 C145(1.29)  LDD0593  [2]
 LDCM0277  AC18 HCT 116 C145(1.04)  LDD0594  [2]
 LDCM0278  AC19 HCT 116 C145(0.89)  LDD0595  [2]
 LDCM0279  AC2 HCT 116 C145(1.36)  LDD0596  [2]
 LDCM0280  AC20 HCT 116 C145(1.08)  LDD0597  [2]
 LDCM0281  AC21 HCT 116 C145(1.24)  LDD0598  [2]
 LDCM0282  AC22 HCT 116 C145(1.56)  LDD0599  [2]
 LDCM0283  AC23 HCT 116 C145(1.44)  LDD0600  [2]
 LDCM0284  AC24 HCT 116 C145(1.57)  LDD0601  [2]
 LDCM0285  AC25 HCT 116 C145(1.53)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C145(1.24)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C145(1.46)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C145(1.39)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C145(1.43)  LDD0606  [2]
 LDCM0290  AC3 HCT 116 C145(1.55)  LDD0607  [2]
 LDCM0291  AC30 HCT 116 C145(2.81)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C145(1.35)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C145(1.37)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C145(2.09)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C145(1.52)  LDD0612  [2]
 LDCM0297  AC36 HEK-293T C42(1.23)  LDD1536  [10]
 LDCM0298  AC37 HEK-293T C499(0.98)  LDD1537  [10]
 LDCM0300  AC39 HEK-293T C42(1.02)  LDD1539  [10]
 LDCM0301  AC4 HCT 116 C145(1.38)  LDD0618  [2]
 LDCM0303  AC41 HEK-293T C42(1.11)  LDD1542  [10]
 LDCM0304  AC42 HEK-293T C42(1.06)  LDD1543  [10]
 LDCM0306  AC44 HEK-293T C42(1.31)  LDD1545  [10]
 LDCM0307  AC45 HEK-293T C499(1.11)  LDD1546  [10]
 LDCM0309  AC47 HEK-293T C42(1.01)  LDD1548  [10]
 LDCM0311  AC49 HEK-293T C42(1.11)  LDD1550  [10]
 LDCM0312  AC5 HCT 116 C145(1.47)  LDD0629  [2]
 LDCM0313  AC50 HEK-293T C42(1.33)  LDD1552  [10]
 LDCM0315  AC52 HEK-293T C42(1.23)  LDD1554  [10]
 LDCM0316  AC53 HEK-293T C499(1.02)  LDD1555  [10]
 LDCM0318  AC55 HEK-293T C42(1.08)  LDD1557  [10]
 LDCM0320  AC57 HEK-293T C42(1.14)  LDD1559  [10]
 LDCM0321  AC58 HEK-293T C42(1.09)  LDD1560  [10]
 LDCM0324  AC60 HEK-293T C42(1.12)  LDD1563  [10]
 LDCM0325  AC61 HEK-293T C499(1.03)  LDD1564  [10]
 LDCM0327  AC63 HEK-293T C42(0.94)  LDD1566  [10]
 LDCM0334  AC7 HEK-293T C42(0.94)  LDD1568  [10]
 LDCM0248  AKOS034007472 HEK-293T C499(1.02)  LDD1511  [10]
 LDCM0356  AKOS034007680 HEK-293T C42(1.12)  LDD1570  [10]
 LDCM0020  ARS-1620 HCC44 C145(1.07)  LDD0078  [2]
 LDCM0632  CL-Sc Hep-G2 C499(1.03)  LDD2227  [4]
 LDCM0369  CL100 HCT 116 C145(1.34)  LDD0686  [2]
 LDCM0374  CL105 HCT 116 C145(1.40)  LDD0691  [2]
 LDCM0375  CL106 HCT 116 C145(2.55)  LDD0692  [2]
 LDCM0376  CL107 HCT 116 C145(1.73)  LDD0693  [2]
 LDCM0377  CL108 HCT 116 C145(2.54)  LDD0694  [2]
 LDCM0378  CL109 HCT 116 C145(1.46)  LDD0695  [2]
 LDCM0379  CL11 HEK-293T C42(1.14)  LDD1583  [10]
 LDCM0380  CL110 HCT 116 C145(2.36)  LDD0697  [2]
 LDCM0381  CL111 HCT 116 C145(1.18)  LDD0698  [2]
 LDCM0382  CL112 HCT 116 C145(1.33)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C145(1.61)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C145(2.29)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C145(1.05)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C145(1.07)  LDD0703  [2]
 LDCM0404  CL17 HEK-293T C42(1.11)  LDD1608  [10]
 LDCM0405  CL18 HEK-293T C42(1.18)  LDD1609  [10]
 LDCM0408  CL20 HEK-293T C42(1.47)  LDD1612  [10]
 LDCM0409  CL21 HEK-293T C499(1.18)  LDD1613  [10]
 LDCM0411  CL23 HEK-293T C42(1.05)  LDD1615  [10]
 LDCM0417  CL29 HEK-293T C42(1.20)  LDD1621  [10]
 LDCM0419  CL30 HEK-293T C42(1.32)  LDD1623  [10]
 LDCM0420  CL31 HCT 116 C145(1.33)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C145(0.86)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C145(1.10)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C145(0.91)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C145(0.89)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C145(0.89)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C145(0.83)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C145(0.68)  LDD0745  [2]
 LDCM0430  CL40 HCT 116 C145(0.61)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C145(1.07)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C145(0.67)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C145(0.69)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C145(0.79)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C145(1.00)  LDD0752  [2]
 LDCM0437  CL47 HEK-293T C42(0.99)  LDD1641  [10]
 LDCM0440  CL5 HEK-293T C42(1.04)  LDD1644  [10]
 LDCM0444  CL53 HEK-293T C42(1.13)  LDD1647  [10]
 LDCM0445  CL54 HEK-293T C42(1.37)  LDD1648  [10]
 LDCM0447  CL56 HEK-293T C42(0.88)  LDD1650  [10]
 LDCM0448  CL57 HEK-293T C499(1.14)  LDD1651  [10]
 LDCM0450  CL59 HEK-293T C42(0.94)  LDD1653  [10]
 LDCM0451  CL6 HEK-293T C42(0.96)  LDD1654  [10]
 LDCM0453  CL61 HCT 116 C145(1.07)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C145(0.95)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C145(1.04)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C145(1.26)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C145(1.03)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C145(1.56)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C145(1.19)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C145(1.27)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C145(1.06)  LDD0778  [2]
 LDCM0463  CL70 HCT 116 C145(1.09)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C145(1.19)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C145(1.04)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C145(1.28)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C145(1.13)  LDD0784  [2]
 LDCM0469  CL76 HCT 116 C145(1.48)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C145(1.29)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C145(0.84)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C145(1.25)  LDD0789  [2]
 LDCM0473  CL8 HEK-293T C42(0.57)  LDD1676  [10]
 LDCM0474  CL80 HCT 116 C145(0.87)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C145(1.25)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C145(1.86)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C145(1.85)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C145(1.62)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C145(1.12)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C145(0.80)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C145(0.99)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C145(1.44)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C145(1.72)  LDD0800  [2]
 LDCM0484  CL9 HEK-293T C499(1.22)  LDD1687  [10]
 LDCM0485  CL90 HCT 116 C145(0.73)  LDD0802  [2]
 LDCM0486  CL91 HCT 116 C145(0.98)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C145(1.11)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C145(0.94)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C145(1.04)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C145(1.09)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C145(1.40)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C145(1.31)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C145(1.19)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C145(1.21)  LDD0811  [2]
 LDCM0468  Fragment33 HCT 116 C145(1.18)  LDD0785  [2]
 LDCM0427  Fragment51 HCT 116 C145(0.55)  LDD0744  [2]
 LDCM0022  KB02 HEK-293T C499(0.90)  LDD1492  [10]
 LDCM0023  KB03 Jurkat C499(4.82)  LDD0209  [11]
 LDCM0024  KB05 OAW-42 C499(2.66)  LDD3369  [12]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Prolyl hydroxylase EGLN3 (EGLN3) . Q9H6Z9
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Forkhead box protein J2 (FOXJ2) . Q9P0K8
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cleavage stimulation factor subunit 2 tau variant (CSTF2T) . Q9H0L4

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
5 Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling. Anal Chem. 2021 Feb 2;93(4):2610-2618. doi: 10.1021/acs.analchem.0c04726. Epub 2021 Jan 20.
Mass spectrometry data entry: PXD022279
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
9 Cell-based proteome profiling of potential dasatinib targets by use of affinity-based probes. J Am Chem Soc. 2012 Feb 15;134(6):3001-14. doi: 10.1021/ja208518u. Epub 2012 Feb 1.
10 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
11 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
12 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840