Details of the Target

General Information of Target

Target ID LDTP11895
Target Name Lck-interacting transmembrane adapter 1 (LIME1)
Gene Name LIME1
Gene ID 54923
Synonyms
LIME; Lck-interacting transmembrane adapter 1; Lck-interacting membrane protein; Lck-interacting molecule
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADQRQRSLSTSGESLYHVLGLDKNATSDDIKKSYRKLALKYHPDKNPDNPEAADKFKEI
NNAHAILTDATKRNIYDKYGSLGLYVAEQFGEENVNTYFVLSSWWAKALFVFCGLLTCCY
CCCCLCCCFNCCCGKCKPKAPEGEETEFYVSPEDLEAQLQSDEREATDTPIVIQPASATE
TTQLTADSHPSYHTDGFN
Target Bioclass
Other
Subcellular location
Cell membrane
Function
Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.
Uniprot ID
Q9H400
Ensemble ID
ENST00000309546.8
HGNC ID
HGNC:26016

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.L81P .
AN3CA SNV: p.P162H .
CAL33 Substitution: p.Q52P .
CHL1 SNV: p.V106G .
IPC298 SNV: p.A152D .
JURKAT Deletion: p.L250WfsTer34
SNV: p.E182Ter		 .
MCC13 SNV: p.G271R .
MOLT4 SNV: p.R173C .
NUGC3 SNV: p.A30P .
RKO SNV: p.G248A .
TOV21G SNV: p.A44V; p.R48W .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C69(2.63)  LDD0304  [1]
DBIA
 Probe Info  		C270(1.10); C280(1.10)  LDD0532  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C270(1.10); C280(1.10)  LDD0532  [2]
 LDCM0216  AC100 HCT 116 C270(0.87); C280(0.87)  LDD0533  [2]
 LDCM0217  AC101 HCT 116 C270(0.84); C280(0.84)  LDD0534  [2]
 LDCM0218  AC102 HCT 116 C270(1.05); C280(1.05)  LDD0535  [2]
 LDCM0219  AC103 HCT 116 C270(1.46); C280(1.46)  LDD0536  [2]
 LDCM0220  AC104 HCT 116 C270(1.10); C280(1.10)  LDD0537  [2]
 LDCM0221  AC105 HCT 116 C270(1.12); C280(1.12)  LDD0538  [2]
 LDCM0222  AC106 HCT 116 C270(1.04); C280(1.04)  LDD0539  [2]
 LDCM0223  AC107 HCT 116 C270(1.21); C280(1.05)  LDD0540  [2]
 LDCM0224  AC108 HCT 116 C270(1.10); C280(0.82)  LDD0541  [2]
 LDCM0225  AC109 HCT 116 C270(0.82); C280(1.24)  LDD0542  [2]
 LDCM0226  AC11 HCT 116 C270(1.57); C280(1.57)  LDD0543  [2]
 LDCM0227  AC110 HCT 116 C270(1.19); C280(0.99)  LDD0544  [2]
 LDCM0228  AC111 HCT 116 C270(1.09); C280(0.88)  LDD0545  [2]
 LDCM0229  AC112 HCT 116 C270(1.06); C280(0.92)  LDD0546  [2]
 LDCM0230  AC113 HCT 116 C270(0.88); C280(0.88)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C270(1.11); C280(1.11)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C270(1.16); C280(1.16)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C270(1.22); C280(1.22)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C270(0.92); C280(0.92)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C270(1.02); C280(1.02)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C270(1.05); C280(1.05)  LDD0553  [2]
 LDCM0237  AC12 HCT 116 C270(1.21); C280(1.21)  LDD0554  [2]
 LDCM0238  AC120 HCT 116 C270(0.95); C280(0.95)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C270(0.92); C280(0.92)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C270(0.84); C280(0.84)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C270(1.00); C280(1.00)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C270(0.85); C280(0.85)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C270(1.07); C280(1.07)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C270(1.35); C280(1.35)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C270(1.19); C280(1.19)  LDD0562  [2]
 LDCM0259  AC14 HCT 116 C270(1.24); C280(1.24)  LDD0576  [2]
 LDCM0263  AC143 HCT 116 C270(0.70); C280(0.70)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C270(0.63); C280(0.63)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C270(0.49); C280(0.49)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C270(0.58); C280(0.58)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C270(0.60); C280(0.60)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C270(0.79); C280(0.79)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C270(0.74); C280(0.74)  LDD0586  [2]
 LDCM0270  AC15 HCT 116 C270(1.20); C280(1.20)  LDD0587  [2]
 LDCM0271  AC150 HCT 116 C270(0.67); C280(0.67)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C270(0.63); C280(0.63)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C270(0.80); C280(0.80)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C270(0.65); C280(0.65)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C270(0.54); C280(0.54)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C270(0.54); C280(0.54)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C270(0.74); C280(0.74)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C270(0.65); C280(0.65)  LDD2161  [2]
 LDCM0296  AC35 HCT 116 C270(0.95); C280(0.95)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C270(0.62); C280(0.62)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C270(0.54); C280(0.54)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C270(0.59); C280(0.59)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C270(0.93); C280(0.93)  LDD0617  [2]
 LDCM0302  AC40 HCT 116 C270(1.01); C280(1.01)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C270(0.76); C280(0.76)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C270(0.77); C280(0.77)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C270(0.74); C280(0.74)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C270(0.71); C280(0.71)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C270(0.94); C280(0.94)  LDD0624  [2]
 LDCM0320  AC57 HCT 116 C270(1.06); C280(1.06)  LDD0637  [2]
 LDCM0321  AC58 HCT 116 C270(1.12); C280(1.12)  LDD0638  [2]
 LDCM0322  AC59 HCT 116 C270(1.31); C280(1.31)  LDD0639  [2]
 LDCM0323  AC6 HCT 116 C270(1.52); C280(1.52)  LDD0640  [2]
 LDCM0324  AC60 HCT 116 C270(1.10); C280(1.10)  LDD0641  [2]
 LDCM0325  AC61 HCT 116 C270(0.96); C280(0.96)  LDD0642  [2]
 LDCM0326  AC62 HCT 116 C270(1.29); C280(1.29)  LDD0643  [2]
 LDCM0327  AC63 HCT 116 C270(1.17); C280(1.17)  LDD0644  [2]
 LDCM0328  AC64 HCT 116 C270(1.21); C280(1.21)  LDD0645  [2]
 LDCM0329  AC65 HCT 116 C270(0.73); C280(0.73)  LDD0646  [2]
 LDCM0330  AC66 HCT 116 C270(0.65); C280(0.65)  LDD0647  [2]
 LDCM0331  AC67 HCT 116 C270(0.81); C280(0.81)  LDD0648  [2]
 LDCM0332  AC68 HCT 116 C270(1.01); C280(1.01)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C270(1.20); C280(1.20)  LDD0650  [2]
 LDCM0334  AC7 HCT 116 C270(1.40); C280(1.40)  LDD0651  [2]
 LDCM0335  AC70 HCT 116 C270(1.15); C280(1.15)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C270(0.86); C280(0.86)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C270(1.30); C280(1.30)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C270(2.00); C280(2.00)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C270(1.40); C280(1.40)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C270(1.77); C280(1.77)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C270(1.33); C280(1.33)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C270(1.57); C280(1.57)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C270(1.15); C280(1.15)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C270(1.11); C280(1.11)  LDD0661  [2]
 LDCM0345  AC8 HCT 116 C270(1.36); C280(1.36)  LDD0662  [2]
 LDCM0346  AC80 HCT 116 C270(1.25); C280(1.25)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C270(0.95); C280(0.95)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C270(1.90); C280(1.90)  LDD0665  [2]
 LDCM0349  AC83 HCT 116 C270(2.05); C280(2.05)  LDD0666  [2]
 LDCM0350  AC84 HCT 116 C270(1.67); C280(1.67)  LDD0667  [2]
 LDCM0351  AC85 HCT 116 C270(1.37); C280(1.37)  LDD0668  [2]
 LDCM0352  AC86 HCT 116 C270(1.31); C280(1.31)  LDD0669  [2]
 LDCM0353  AC87 HCT 116 C270(1.14); C280(1.14)  LDD0670  [2]
 LDCM0354  AC88 HCT 116 C270(1.45); C280(1.45)  LDD0671  [2]
 LDCM0355  AC89 HCT 116 C270(1.80); C280(1.80)  LDD0672  [2]
 LDCM0357  AC90 HCT 116 C270(1.09); C280(1.09)  LDD0674  [2]
 LDCM0358  AC91 HCT 116 C270(2.20); C280(2.20)  LDD0675  [2]
 LDCM0359  AC92 HCT 116 C270(2.15); C280(2.15)  LDD0676  [2]
 LDCM0360  AC93 HCT 116 C270(1.27); C280(1.27)  LDD0677  [2]
 LDCM0361  AC94 HCT 116 C270(1.37); C280(1.37)  LDD0678  [2]
 LDCM0362  AC95 HCT 116 C270(1.36); C280(1.36)  LDD0679  [2]
 LDCM0363  AC96 HCT 116 C270(1.90); C280(1.90)  LDD0680  [2]
 LDCM0364  AC97 HCT 116 C270(2.16); C280(2.16)  LDD0681  [2]
 LDCM0365  AC98 HCT 116 C270(2.37); C280(2.37)  LDD0682  [2]
 LDCM0366  AC99 HCT 116 C270(0.77); C280(0.77)  LDD0683  [2]
 LDCM0248  AKOS034007472 HCT 116 C270(1.44); C280(1.44)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C270(1.25); C280(1.25)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C270(1.62); C280(1.62)  LDD0592  [2]
 LDCM0020  ARS-1620 HCC44 C270(1.02); C280(1.02)  LDD2171  [2]
 LDCM0370  CL101 HCT 116 C270(1.72); C280(1.72)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C270(1.42); C280(1.42)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C270(1.76); C280(1.76)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C270(1.38); C280(1.38)  LDD0690  [2]
 LDCM0387  CL117 HCT 116 C270(0.97); C280(0.97)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C270(0.72); C280(0.72)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C270(0.88); C280(0.88)  LDD0706  [2]
 LDCM0391  CL120 HCT 116 C270(1.05); C280(1.05)  LDD0708  [2]
 LDCM0396  CL125 HCT 116 C270(0.93); C280(0.93)  LDD0713  [2]
 LDCM0397  CL126 HCT 116 C270(0.94); C280(0.94)  LDD0714  [2]
 LDCM0398  CL127 HCT 116 C270(0.77); C280(0.77)  LDD0715  [2]
 LDCM0399  CL128 HCT 116 C270(1.21); C280(1.21)  LDD0716  [2]
 LDCM0436  CL46 HCT 116 C270(1.34); C280(1.34)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C270(0.95); C280(0.95)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C270(0.73); C280(0.73)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C270(0.72); C280(0.72)  LDD0756  [2]
 LDCM0441  CL50 HCT 116 C270(0.87); C280(0.87)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C270(0.98); C280(0.98)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C270(1.72); C280(1.72)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C270(1.06); C280(1.06)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C270(1.09); C280(1.09)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C270(1.08); C280(1.08)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C270(0.91); C280(0.91)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C270(0.85); C280(0.85)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C270(1.07); C280(1.07)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C270(0.74); C280(0.74)  LDD0767  [2]
 LDCM0452  CL60 HCT 116 C270(0.92); C280(0.92)  LDD0769  [2]
 LDCM0202  EV-93 T cell C69(4.28)  LDD0514  [3]
 LDCM0625  F8 Ramos C69(0.88)  LDD2187  [4]
 LDCM0573  Fragment11 Ramos C69(15.15)  LDD2190  [4]
 LDCM0586  Fragment28 Ramos C69(0.56)  LDD2198  [4]
 LDCM0566  Fragment4 Ramos C69(2.33)  LDD2184  [4]
 LDCM0022  KB02 T cell C270(5.33); C280(0.00)  LDD1703  [3]
 LDCM0024  KB05 T cell C69(13.93)  LDD1709  [3]
 LDCM0131  RA190 MM1.R C69(2.63)  LDD0304  [1]
 LDCM0021  THZ1 HCT 116 C270(1.02); C280(1.02)  LDD2173  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mesoderm-specific transcript homolog protein (MEST) AB hydrolase superfamily Q5EB52
Anterior gradient protein 3 (AGR3) AGR family Q8TD06
FAST kinase domain-containing protein 4 (TBRG4) FAST kinase family Q969Z0
Epididymis-specific alpha-mannosidase (MAN2B2) Glycosyl hydrolase 38 family Q9Y2E5
Glutamate decarboxylase 2 (GAD2) Group II decarboxylase family Q05329
Tyrosine-protein phosphatase non-receptor type 9 (PTPN9) Protein-tyrosine phosphatase family P43378
Pyridoxine-5'-phosphate oxidase (PNPO) Pyridoxamine 5'-phosphate oxidase family Q9NVS9
Lysoplasmalogenase TMEM86B (TMEM86B) TMEM86 family Q8N661
BCL2/adenovirus E1B 19 kDa protein-interacting protein 2 (BNIP2) . Q12982
Transporter and channel
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bax inhibitor 1 (TMBIM6) BI1 family P55061
LHFPL tetraspan subfamily member 5 protein (LHFPL5) LHFP family Q8TAF8
Aquaporin-1 (AQP1) MIP/aquaporin (TC 1.A.8) family P29972
Ninjurin-1 (NINJ1) Ninjurin family Q92982
Solute carrier family 41 member 2 (SLC41A2) SLC41A transporter family Q96JW4
Sorting nexin-1 (SNX1) Sorting nexin family Q13596
Syntaxin-6 (STX6) Syntaxin family O43752
Transmembrane protein 74 (TMEM74) TMEM74 family Q96NL1
Sigma intracellular receptor 2 (TMEM97) TMEM97/sigma-2 receptor family Q5BJF2
Protein unc-93 homolog B1 (UNC93B1) Unc-93 family Q9H1C4
Uroplakin-2 (UPK2) Uroplakin-2 family O00526
Ceroid-lipofuscinosis neuronal protein 6 (CLN6) . Q9NWW5
GPCR
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Lysophosphatidic acid receptor 3 (LPAR3) G-protein coupled receptor 1 family Q9UBY5
Other
Click To Hide/Show 15 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Golgi SNAP receptor complex member 2 (GOSR2) GOSR2 family O14653
Cytosolic iron-sulfur assembly component 2A (CIAO2A) MIP18 family Q9H5X1
Synaptosomal-associated protein 47 (SNAP47) SVAP1 family Q5SQN1
Vesicle-trafficking protein SEC22c (SEC22C) Synaptobrevin family Q9BRL7
Syntaxin-7 (STX7) Syntaxin family O15400
Transmembrane protein 243 (TMEM243) TMEM243 family Q9BU79
Receptor-transporting protein 2 (RTP2) TMEM7 family Q5QGT7
Zinc finger protein-like 1 (ZFPL1) ZFPL1 family O95159
ADP-ribosylation factor GTPase-activating protein 3 (ARFGAP3) . Q9NP61
Calcium-binding protein 8 (CALN1) . Q9BXU9
Emerin (EMD) . P50402
FERM domain-containing protein 8 (FRMD8) . Q9BZ67
Nutritionally-regulated adipose and cardiac enriched protein homolog (NRAC) . Q8N912
TRAF3-interacting JNK-activating modulator (TRAF3IP3) . Q9Y228
Transmembrane protein 143 (TMEM143) . Q96AN5

References

1 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
4 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578