Details of the Target

General Information of Target

Target ID LDTP11873
Target Name Thioredoxin-interacting protein (TXNIP)
Gene Name TXNIP
Gene ID 10628
Synonyms
VDUP1; Thioredoxin-interacting protein; Thioredoxin-binding protein 2; Vitamin D3 up-regulated protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDGSGERSLPEPGSQSSAASDDIEIVVNVGGVRQVLYGDLLSQYPETRLAELINCLAGGY
DTIFSLCDDYDPGKREFYFDRDPDAFKCVIEVYYFGEVHMKKGICPICFKNEMDFWKVDL
KFLDDCCKSHLSEKREELEEIARRVQLILDDLGVDAAEGRWRRCQKCVWKFLEKPESSCP
ARVVAVLSFLLILVSSVVMCMGTIPELQVLDAEGNRVEHPTLENVETACIGWFTLEYLLR
LFSSPNKLHFALSFMNIVDVLAILPFYVSLTLTHLGARMMELTNVQQAVQALRIMRIARI
FKLARHSSGLQTLTYALKRSFKELGLLLMYLAVGIFVFSALGYTMEQSHPETLFKSIPQS
FWWAIITMTTVGYGDIYPKTTLGKLNAAISFLCGVIAIALPIHPIINNFVRYYNKQRVLE
TAAKHELELMELNSSSGGEGKTGGSRSDLDNLPPEPAGKEAPSCSSRLKLSHSDTFIPLL
TEEKHHRTRLQSCK
Target Type
Literature-reported
Target Bioclass
Transporter and channel
Family
Arrestin family
Subcellular location
Cytoplasm
Function
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).
TTD ID
T77127
Uniprot ID
Q9H3M7
DrugMap ID
TTTLDZK
Ensemble ID
ENST00000425134.2
HGNC ID
HGNC:16952

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 SNV: p.Q232R DBIA    Probe Info 
HCT15 SNV: p.I363N DBIA    Probe Info 
Ishikawa (Heraklio) 02 ER Deletion: p.E165KfsTer66 DBIA    Probe Info 
NCIH2172 SNV: p.E18Q DBIA    Probe Info 
SKNAS SNV: p.A217S DBIA    Probe Info 
TKKK Insertion: p.R177AfsTer9 DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K115(7.14); K167(10.00); K233(10.00); K287(2.86)  LDD0277  [1]
Probe 1
 Probe Info  		Y69(13.41); Y221(16.43)  LDD3495  [2]
IPM
 Probe Info  		C49(4.97)  LDD1701  [3]
DBIA
 Probe Info  		C247(1.38); C120(0.87)  LDD0078  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [5]
TFBX
 Probe Info  		N.A.  LDD0027  [6]
Acrolein
 Probe Info  		C170(0.00); C36(0.00)  LDD0217  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0230  AC113 PaTu 8988t C120(0.89)  LDD1109  [4]
 LDCM0231  AC114 PaTu 8988t C120(0.86)  LDD1110  [4]
 LDCM0232  AC115 PaTu 8988t C120(1.10)  LDD1111  [4]
 LDCM0233  AC116 PaTu 8988t C120(0.90)  LDD1112  [4]
 LDCM0234  AC117 PaTu 8988t C120(0.86)  LDD1113  [4]
 LDCM0235  AC118 PaTu 8988t C120(0.88)  LDD1114  [4]
 LDCM0236  AC119 PaTu 8988t C120(1.07)  LDD1115  [4]
 LDCM0238  AC120 PaTu 8988t C120(0.79)  LDD1117  [4]
 LDCM0239  AC121 PaTu 8988t C120(0.78)  LDD1118  [4]
 LDCM0240  AC122 PaTu 8988t C120(0.77)  LDD1119  [4]
 LDCM0241  AC123 PaTu 8988t C120(0.77)  LDD1120  [4]
 LDCM0242  AC124 PaTu 8988t C120(0.84)  LDD1121  [4]
 LDCM0243  AC125 PaTu 8988t C120(0.95)  LDD1122  [4]
 LDCM0244  AC126 PaTu 8988t C120(0.78)  LDD1123  [4]
 LDCM0245  AC127 PaTu 8988t C120(0.98)  LDD1124  [4]
 LDCM0296  AC35 PaTu 8988t C120(0.99)  LDD1175  [4]
 LDCM0297  AC36 PaTu 8988t C120(1.03)  LDD1176  [4]
 LDCM0298  AC37 PaTu 8988t C120(1.21)  LDD1177  [4]
 LDCM0299  AC38 PaTu 8988t C120(1.03)  LDD1178  [4]
 LDCM0300  AC39 PaTu 8988t C120(1.05)  LDD1179  [4]
 LDCM0302  AC40 PaTu 8988t C120(1.18)  LDD1181  [4]
 LDCM0303  AC41 PaTu 8988t C120(1.06)  LDD1182  [4]
 LDCM0304  AC42 PaTu 8988t C120(1.18)  LDD1183  [4]
 LDCM0305  AC43 PaTu 8988t C120(0.94)  LDD1184  [4]
 LDCM0306  AC44 PaTu 8988t C120(1.26)  LDD1185  [4]
 LDCM0307  AC45 PaTu 8988t C120(1.29)  LDD1186  [4]
 LDCM0020  ARS-1620 HCC44 C247(1.38); C120(0.87)  LDD0078  [4]
 LDCM0103  BDHI 10 Jurkat C49(11.35)  LDD0205  [9]
 LDCM0102  BDHI 8 Jurkat C49(11.35)  LDD0204  [9]
 LDCM0199  BPK-21 T cell C170(14.44)  LDD0517  [10]
 LDCM0108  Chloroacetamide HeLa C170(0.00); C247(0.00)  LDD0222  [7]
 LDCM0632  CL-Sc Hep-G2 C120(20.00); C247(1.70); C170(1.54); C36(0.83)  LDD2227  [8]
 LDCM0367  CL1 PaTu 8988t C120(1.09)  LDD1246  [4]
 LDCM0368  CL10 PaTu 8988t C120(1.20)  LDD1247  [4]
 LDCM0379  CL11 PaTu 8988t C120(0.99)  LDD1258  [4]
 LDCM0387  CL117 PaTu 8988t C120(1.07)  LDD1266  [4]
 LDCM0388  CL118 PaTu 8988t C120(1.32)  LDD1267  [4]
 LDCM0389  CL119 PaTu 8988t C120(1.04)  LDD1268  [4]
 LDCM0390  CL12 PaTu 8988t C120(1.09)  LDD1269  [4]
 LDCM0391  CL120 PaTu 8988t C120(1.00)  LDD1270  [4]
 LDCM0400  CL13 PaTu 8988t C120(1.12)  LDD1279  [4]
 LDCM0401  CL14 PaTu 8988t C120(1.03)  LDD1280  [4]
 LDCM0402  CL15 PaTu 8988t C120(1.05)  LDD1281  [4]
 LDCM0407  CL2 PaTu 8988t C120(0.99)  LDD1286  [4]
 LDCM0418  CL3 PaTu 8988t C120(1.07)  LDD1297  [4]
 LDCM0420  CL31 PaTu 8988t C120(1.09)  LDD1299  [4]
 LDCM0421  CL32 PaTu 8988t C120(1.04)  LDD1300  [4]
 LDCM0422  CL33 PaTu 8988t C120(1.63)  LDD1301  [4]
 LDCM0423  CL34 PaTu 8988t C120(1.10)  LDD1302  [4]
 LDCM0424  CL35 PaTu 8988t C120(1.25)  LDD1303  [4]
 LDCM0425  CL36 PaTu 8988t C120(1.31)  LDD1304  [4]
 LDCM0426  CL37 PaTu 8988t C120(1.43)  LDD1305  [4]
 LDCM0428  CL39 PaTu 8988t C120(1.32)  LDD1307  [4]
 LDCM0429  CL4 PaTu 8988t C120(0.88)  LDD1308  [4]
 LDCM0430  CL40 PaTu 8988t C120(1.20)  LDD1309  [4]
 LDCM0431  CL41 PaTu 8988t C120(1.25)  LDD1310  [4]
 LDCM0432  CL42 PaTu 8988t C120(1.54)  LDD1311  [4]
 LDCM0433  CL43 PaTu 8988t C120(1.93)  LDD1312  [4]
 LDCM0434  CL44 PaTu 8988t C120(1.35)  LDD1313  [4]
 LDCM0435  CL45 PaTu 8988t C120(1.05)  LDD1314  [4]
 LDCM0436  CL46 PaTu 8988t C120(0.98)  LDD1315  [4]
 LDCM0437  CL47 PaTu 8988t C120(1.10)  LDD1316  [4]
 LDCM0438  CL48 PaTu 8988t C120(0.99)  LDD1317  [4]
 LDCM0439  CL49 PaTu 8988t C120(1.04)  LDD1318  [4]
 LDCM0440  CL5 PaTu 8988t C120(0.92)  LDD1319  [4]
 LDCM0441  CL50 PaTu 8988t C120(1.04)  LDD1320  [4]
 LDCM0442  CL51 PaTu 8988t C120(1.12)  LDD1321  [4]
 LDCM0443  CL52 PaTu 8988t C120(1.01)  LDD1322  [4]
 LDCM0444  CL53 PaTu 8988t C120(1.07)  LDD1323  [4]
 LDCM0445  CL54 PaTu 8988t C120(0.95)  LDD1324  [4]
 LDCM0446  CL55 PaTu 8988t C120(1.01)  LDD1325  [4]
 LDCM0447  CL56 PaTu 8988t C120(0.19)  LDD1326  [4]
 LDCM0448  CL57 PaTu 8988t C120(1.71)  LDD1327  [4]
 LDCM0449  CL58 PaTu 8988t C120(1.45)  LDD1328  [4]
 LDCM0450  CL59 PaTu 8988t C120(1.61)  LDD1329  [4]
 LDCM0451  CL6 PaTu 8988t C120(1.09)  LDD1330  [4]
 LDCM0452  CL60 PaTu 8988t C120(1.30)  LDD1331  [4]
 LDCM0462  CL7 PaTu 8988t C120(1.06)  LDD1341  [4]
 LDCM0469  CL76 PaTu 8988t C120(0.79)  LDD1348  [4]
 LDCM0470  CL77 PaTu 8988t C120(0.99)  LDD1349  [4]
 LDCM0471  CL78 PaTu 8988t C120(1.03)  LDD1350  [4]
 LDCM0472  CL79 PaTu 8988t C120(0.92)  LDD1351  [4]
 LDCM0473  CL8 PaTu 8988t C120(1.18)  LDD1352  [4]
 LDCM0474  CL80 PaTu 8988t C120(1.17)  LDD1353  [4]
 LDCM0475  CL81 PaTu 8988t C120(1.16)  LDD1354  [4]
 LDCM0476  CL82 PaTu 8988t C120(1.08)  LDD1355  [4]
 LDCM0477  CL83 PaTu 8988t C120(0.88)  LDD1356  [4]
 LDCM0478  CL84 PaTu 8988t C120(1.37)  LDD1357  [4]
 LDCM0479  CL85 PaTu 8988t C120(0.98)  LDD1358  [4]
 LDCM0480  CL86 PaTu 8988t C120(1.14)  LDD1359  [4]
 LDCM0481  CL87 PaTu 8988t C120(1.33)  LDD1360  [4]
 LDCM0482  CL88 PaTu 8988t C120(1.23)  LDD1361  [4]
 LDCM0483  CL89 PaTu 8988t C120(1.10)  LDD1362  [4]
 LDCM0484  CL9 PaTu 8988t C120(0.91)  LDD1363  [4]
 LDCM0485  CL90 PaTu 8988t C120(1.47)  LDD1364  [4]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C49(3.20)  LDD1702  [3]
 LDCM0580  Fragment21 MDA-MB-231 C170(2.14)  LDD1404  [11]
 LDCM0584  Fragment25 MDA-MB-231 C170(1.18)  LDD1411  [11]
 LDCM0578  Fragment27 MDA-MB-231 C170(1.31)  LDD1413  [11]
 LDCM0587  Fragment29 MDA-MB-231 C170(1.20)  LDD1417  [11]
 LDCM0589  Fragment31 MDA-MB-231 C170(2.46)  LDD1421  [11]
 LDCM0590  Fragment32 MDA-MB-231 C170(2.17)  LDD1423  [11]
 LDCM0596  Fragment38 MDA-MB-231 C170(1.73)  LDD1433  [11]
 LDCM0566  Fragment4 MDA-MB-231 C170(20.00)  LDD1378  [11]
 LDCM0601  Fragment43 MDA-MB-231 C170(2.93)  LDD1441  [11]
 LDCM0604  Fragment46 MDA-MB-231 C170(1.13)  LDD1445  [11]
 LDCM0427  Fragment51 PaTu 8988t C120(1.65)  LDD1306  [4]
 LDCM0610  Fragment52 MDA-MB-231 C170(2.43)  LDD1452  [11]
 LDCM0614  Fragment56 MDA-MB-231 C170(1.14)  LDD1458  [11]
 LDCM0571  Fragment9 MDA-MB-231 C170(3.12)  LDD1387  [11]
 LDCM0022  KB02 HCT 116 C120(1.52)  LDD0080  [4]
 LDCM0023  KB03 HCT 116 C120(1.21)  LDD0081  [4]
 LDCM0024  KB05 HCT 116 C120(2.35)  LDD0082  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Thioredoxin (TXN) Thioredoxin family P10599
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Guanine nucleotide exchange factor VAV2 (VAV2) . P52735
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein ecdysoneless homolog (ECD) ECD family O95905

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
10 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
11 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.