Details of the Target

General Information of Target

Target ID LDTP11562
Target Name E3 ubiquitin-protein ligase TRIM8 (TRIM8)
Gene Name TRIM8
Gene ID 81603
Synonyms
GERP; RNF27; E3 ubiquitin-protein ligase TRIM8; EC 2.3.2.27; Glioblastoma-expressed RING finger protein; RING finger protein 27; RING-type E3 ubiquitin transferase TRIM8; Tripartite motif-containing protein 8
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MCSTSGCDLEEIPLDDDDLNTIEFKILAYYTRHHVFKSTPALFSPKLLRTRSLSQRGLGN
CSANESWTEVSWPCRNSQSSEKAINLGKKKSSWKAFFGVVEKEDSQSTPAKVSAQGQRTL
EYQDSHSQQWSRCLSNVEQCLEHEAVDPKVISIANRVAEIVYSWPPPQATQAGGFKSKEI
FVTEGLSFQLQGHVPVASSSKKDEEEQILAKIVELLKYSGDQLERKLKKDKALMGHFQDG
LSYSVFKTITDQVLMGVDPRGESEVKAQGFKAALVIDVTAKLTAIDNHPMNRVLGFGTKY
LKENFSPWIQQHGGWEKILGISHEEVD
Target Bioclass
Enzyme
Family
TRIM/RBCC family
Subcellular location
Cytoplasm
Function
E3 ubiquitin-protein ligase that participates in multiple biological processes including cell survival, differentiation, apoptosis, and in particular, the innate immune response. Participates in the activation of interferon-gamma signaling by promoting proteasomal degradation of the repressor SOCS1. Plays a positive role in the TNFalpha and IL-1beta signaling pathways. Mechanistically, induces the 'Lys-63'-linked polyubiquitination of MAP3K7/TAK1 component leading to the activation of NF-kappa-B. Modulates also STAT3 activity through negative regulation of PIAS3, either by degradation of PIAS3 through the ubiquitin-proteasome pathway or exclusion of PIAS3 from the nucleus. Negatively regulates TLR3/4-mediated innate immune response by catalyzing 'Lys-6'- and 'Lys-33'-linked polyubiquitination of TICAM1 and thereby disrupting the TICAM1-TBK1 interaction.
Uniprot ID
Q9BZR9
Ensemble ID
ENST00000643721.2
HGNC ID
HGNC:15579

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C110(0.85); C114(0.85); C115(0.85)  LDD0078  [1]
NAIA_5
 Probe Info  		N.A.  LDD2223  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C110(0.84); C114(0.84); C115(0.84)  LDD0531  [1]
 LDCM0216  AC100 HCT 116 C110(0.74); C114(0.66); C115(0.66)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C110(0.83); C114(0.72); C115(0.72)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C110(0.57); C114(0.55); C115(0.55)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C110(0.84); C114(0.85); C115(0.85)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C110(0.70); C114(0.65); C115(0.65)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C110(0.85); C114(0.77); C115(0.77)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C110(1.27); C114(1.08); C115(1.08)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C110(0.88); C114(0.89); C115(0.71)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C110(1.14); C114(0.55); C115(0.94)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C110(1.41); C114(0.62); C115(0.98)  LDD0542  [1]
 LDCM0227  AC110 HCT 116 C110(0.69); C114(0.63); C115(1.05)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C110(0.61); C114(0.68); C115(0.74)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C110(0.63); C114(0.77); C115(0.86)  LDD0546  [1]
 LDCM0230  AC113 HCT 116 C114(0.89); C115(0.89)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C114(1.13); C115(1.13)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C114(1.30); C115(1.30)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C114(0.97); C115(0.97)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C114(1.30); C115(1.30)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C114(1.05); C115(1.05)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C114(1.07); C115(1.07)  LDD0553  [1]
 LDCM0238  AC120 HCT 116 C114(0.80); C115(0.80)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C114(0.93); C115(0.93)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C114(1.00); C115(1.00)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C114(1.04); C115(1.04)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C114(1.31); C115(1.31)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C114(1.15); C115(1.15)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C114(1.25); C115(1.25)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C114(1.22); C115(1.22)  LDD0562  [1]
 LDCM0263  AC143 HCT 116 C110(0.82); C114(0.92); C115(0.92)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C110(1.11); C114(1.16); C115(1.16)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C110(0.89); C114(0.95); C115(0.95)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C110(1.02); C114(1.16); C115(1.16)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C110(1.08); C114(1.37); C115(1.37)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C110(1.19); C114(1.67); C115(1.67)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C110(1.20); C114(1.37); C115(1.37)  LDD0586  [1]
 LDCM0271  AC150 HCT 116 C110(0.99); C114(1.01); C115(1.01)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C110(0.81); C114(0.90); C115(0.90)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C110(1.01); C114(1.09); C115(1.09)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C110(1.38); C114(1.80); C115(1.80)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C110(1.26); C114(1.13); C115(1.13)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C110(1.03); C114(1.25); C115(1.25)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C110(0.72); C114(0.97); C115(0.97)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C110(1.03); C114(0.99); C115(0.99)  LDD2161  [1]
 LDCM0279  AC2 HCT 116 C110(1.04); C114(1.04); C115(1.04)  LDD0596  [1]
 LDCM0290  AC3 HCT 116 C110(0.90); C114(0.90); C115(0.90)  LDD0607  [1]
 LDCM0301  AC4 HCT 116 C110(0.97); C114(0.97); C115(0.97)  LDD0618  [1]
 LDCM0312  AC5 HCT 116 C110(0.99); C114(0.99); C115(0.99)  LDD0629  [1]
 LDCM0332  AC68 HCT 116 C110(1.29); C114(1.54); C115(1.79)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C110(1.03); C114(1.24); C115(1.44)  LDD0650  [1]
 LDCM0335  AC70 HCT 116 C110(1.76); C114(1.78); C115(1.80)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C110(1.14); C114(1.20); C115(1.26)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C115(1.20); C114(1.25); C110(1.30)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C110(1.74); C114(1.76); C115(1.79)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C110(2.03); C114(2.20); C115(2.38)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C110(1.68); C114(2.28); C115(2.88)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C110(1.64); C114(1.65); C115(1.65)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C110(1.90); C114(1.94); C115(1.98)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C115(1.03); C114(1.09); C110(1.15)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C115(1.25); C114(1.41); C110(1.57)  LDD0661  [1]
 LDCM0346  AC80 HCT 116 C115(1.22); C114(1.35); C110(1.48)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C110(1.09); C114(1.45); C115(1.82)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C115(2.21); C114(2.35); C110(2.48)  LDD0665  [1]
 LDCM0349  AC83 HCT 116 C110(1.64); C115(1.64); C114(1.64)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C110(1.44); C115(1.44); C114(1.48)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C114(1.16); C110(1.17); C115(1.17)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C114(0.98); C110(1.04); C115(1.04)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C110(0.97); C115(0.97); C114(1.05)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C110(1.12); C115(1.12); C114(1.17)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C110(1.22); C115(1.22); C114(1.39)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C114(1.21); C110(1.24); C115(1.24)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C114(1.57); C110(1.58); C115(1.58)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C110(1.29); C115(1.29); C114(1.38)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C114(1.04); C110(1.04); C115(1.04)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C110(1.04); C115(1.04); C114(1.05)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C114(1.14); C110(1.18); C115(1.18)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C114(1.32); C110(1.34); C115(1.34)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C110(1.12); C115(1.12); C114(1.18)  LDD0681  [1]
 LDCM0365  AC98 HCT 116 C110(0.99); C114(1.03); C115(1.03)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C110(0.57); C114(0.60); C115(0.60)  LDD0683  [1]
 LDCM0020  ARS-1620 HCC44 C110(0.85); C114(0.85); C115(0.85)  LDD0078  [1]
 LDCM0367  CL1 HCT 116 C110(1.13); C114(1.13); C115(1.13)  LDD0684  [1]
 LDCM0368  CL10 HCT 116 C110(0.99); C114(0.99); C115(0.99)  LDD0685  [1]
 LDCM0369  CL100 HCT 116 C110(0.98); C114(0.98); C115(0.98)  LDD0686  [1]
 LDCM0379  CL11 HCT 116 C110(0.71); C114(0.71); C115(0.71)  LDD0696  [1]
 LDCM0390  CL12 HCT 116 C110(1.42); C114(1.42); C115(1.42)  LDD0707  [1]
 LDCM0400  CL13 HCT 116 C110(0.85); C114(0.85); C115(0.85)  LDD0717  [1]
 LDCM0401  CL14 HCT 116 C110(1.20); C114(1.20); C115(1.20)  LDD0718  [1]
 LDCM0402  CL15 HCT 116 C110(1.33); C114(1.33); C115(1.33)  LDD0719  [1]
 LDCM0407  CL2 HCT 116 C110(1.08); C114(1.08); C115(1.08)  LDD0724  [1]
 LDCM0418  CL3 HCT 116 C110(1.02); C114(1.02); C115(1.02)  LDD0735  [1]
 LDCM0420  CL31 HCT 116 C110(0.86); C114(0.86); C115(0.86)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C110(1.25); C114(1.25); C115(1.25)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C110(1.03); C114(1.03); C115(1.03)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C110(1.31); C114(1.31); C115(1.31)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C110(1.16); C114(1.16); C115(1.16)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C110(1.25); C114(1.25); C115(1.25)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C110(1.23); C114(1.23); C115(1.23)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C110(1.54); C114(1.54); C115(1.54)  LDD0745  [1]
 LDCM0429  CL4 HCT 116 C110(1.06); C114(1.06); C115(1.06)  LDD0746  [1]
 LDCM0430  CL40 HCT 116 C110(0.94); C114(0.94); C115(0.94)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C110(1.29); C114(1.29); C115(1.29)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C110(1.51); C114(1.51); C115(1.51)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C110(1.37); C114(1.37); C115(1.37)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C110(1.06); C114(1.06); C115(1.06)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C110(1.12); C114(1.12); C115(1.12)  LDD0752  [1]
 LDCM0436  CL46 HCT 116 C110(0.96); C114(0.96); C115(0.96)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C110(0.86); C114(0.86); C115(0.86)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C110(0.93); C114(0.93); C115(0.93)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C110(0.97); C114(0.97); C115(0.97)  LDD0756  [1]
 LDCM0440  CL5 HCT 116 C110(1.02); C114(1.02); C115(1.02)  LDD0757  [1]
 LDCM0441  CL50 HCT 116 C110(1.11); C114(1.11); C115(1.11)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C110(1.05); C114(1.05); C115(1.05)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C110(1.18); C114(1.18); C115(1.18)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C110(1.16); C114(1.16); C115(1.16)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C110(0.93); C114(0.93); C115(0.93)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C110(1.01); C114(1.01); C115(1.01)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C110(1.01); C114(1.01); C115(1.01)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C110(1.12); C114(1.12); C115(1.12)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C110(1.14); C114(1.14); C115(1.14)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C110(1.35); C114(1.35); C115(1.35)  LDD0767  [1]
 LDCM0451  CL6 HCT 116 C110(1.12); C114(1.12); C115(1.12)  LDD0768  [1]
 LDCM0452  CL60 HCT 116 C110(1.13); C114(1.13); C115(1.13)  LDD0769  [1]
 LDCM0462  CL7 HCT 116 C110(0.95); C114(0.95); C115(0.95)  LDD0779  [1]
 LDCM0473  CL8 HCT 116 C110(1.17); C114(1.17); C115(1.17)  LDD0790  [1]
 LDCM0484  CL9 HCT 116 C110(0.95); C114(0.95); C115(0.95)  LDD0801  [1]
 LDCM0486  CL91 HCT 116 C110(0.95); C114(0.95); C115(0.95)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C110(1.02); C114(1.02); C115(1.02)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C110(0.80); C114(0.80); C115(0.80)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C110(1.20); C114(1.20); C115(1.20)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C110(1.14); C114(1.14); C115(1.14)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C110(1.01); C114(1.01); C115(1.01)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C110(0.93); C114(0.93); C115(0.93)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C110(1.04); C114(1.04); C115(1.04)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C110(0.80); C114(0.80); C115(0.80)  LDD0811  [1]
 LDCM0427  Fragment51 HCT 116 C110(1.56); C114(1.56); C115(1.56)  LDD0744  [1]
 LDCM0022  KB02 42-MG-BA C38(1.16)  LDD2244  [3]
 LDCM0023  KB03 42-MG-BA C38(1.82)  LDD2661  [3]
 LDCM0024  KB05 IGR37 C38(1.26)  LDD3314  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 12 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Diphosphoinositol polyphosphate phosphohydrolase 3-alpha (NUDT10) Nudix hydrolase family Q8NFP7
Ubiquitin thioesterase OTUB2 (OTUB2) Peptidase C65 family Q96DC9
Tensin-2 (TNS2) PTEN phosphatase protein family Q63HR2
E3 ubiquitin-protein ligase RNF168 (RNF168) RNF168 family Q8IYW5
E3 ubiquitin-protein ligase TRIM8 (TRIM8) TRIM/RBCC family Q9BZR9
Ubiquitin-conjugating enzyme E2 D1 (UBE2D1) Ubiquitin-conjugating enzyme family P51668
Ubiquitin-conjugating enzyme E2 D4 (UBE2D4) Ubiquitin-conjugating enzyme family Q9Y2X8
Ubiquitin/ISG15-conjugating enzyme E2 L6 (UBE2L6) Ubiquitin-conjugating enzyme family O14933
DCN1-like protein 1 (DCUN1D1) . Q96GG9
E3 ubiquitin-protein ligase LNX (LNX1) . Q8TBB1
LON peptidase N-terminal domain and RING finger protein 1 (LONRF1) . Q17RB8
Ubiquitin thioesterase OTU1 (YOD1) . Q5VVQ6
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cation channel sperm-associated protein 1 (CATSPER1) Cation channel sperm-associated (TC 1.A.1.19) family Q8NEC5
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homeobox protein Hox-A1 (HOXA1) Antp homeobox family P49639
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Maturin (MTURN) MTURN family Q8N3F0
UV excision repair protein RAD23 homolog A (RAD23A) RAD23 family P54725
TAR DNA-binding protein 43 (TARDBP) . Q13148
TNFAIP3-interacting protein 3 (TNIP3) . Q96KP6
UBX domain-containing protein 7 (UBXN7) . O94888

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840