Details of the Target

General Information of Target

Target ID LDTP11117
Target Name Serine/arginine-rich splicing factor 8 (SRSF8)
Gene Name SRSF8
Gene ID 10929
Synonyms
SFRS2B; SRP46; Serine/arginine-rich splicing factor 8; Pre-mRNA-splicing factor SRP46; Splicing factor SRp46; Splicing factor, arginine/serine-rich 2B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVWPWVAMASRWGPLIGLAPCCLWLLGAVLLMDASARPANHSSTRERVANREENEILPPD
HLNGVKLEMDGHLNRGFHQEVFLGKDLGGFDEDAEPRRSRRKLMVIFSKVDVNTDRKISA
KEMQRWIMEKTAEHFQEAMEESKTHFRAVDPDGDGHVSWDEYKVKFLASKGHSEKEVADA
IRLNEELKVDEETQEVLENLKDRWYQADSPPADLLLTEEEFLSFLHPEHSRGMLRFMVKE
IVRDLDQDGDKQLSVPEFISLPVGTVENQQGQDIDDNWVKDRKKEFEELIDSNHDGIVTA
EELESYMDPMNEYNALNEAKQMIAVADENQNHHLEPEEVLKYSEFFTGSKLVDYARSVHE
EF
Target Bioclass
Other
Family
Splicing factor SR family
Subcellular location
Nucleus
Function Involved in pre-mRNA alternative splicing.
Uniprot ID
Q9BRL6
Ensemble ID
ENST00000587424.3
HGNC ID
HGNC:16988

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
AGS SNV: p.F62L .
AN3CA Deletion: p.P7LfsTer7 .
HEC1 Substitution: p.P128Y DBIA    Probe Info 
HEC1B Substitution: p.P128Y .
RPMI8226 SNV: p.S210L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C141(1.29)  LDD3332  [1]
HHS-482
 Probe Info  		Y44(0.96)  LDD0285  [2]
HHS-475
 Probe Info  		Y23(0.38); Y44(1.05)  LDD0264  [3]
HHS-465
 Probe Info  		Y23(7.71); Y44(6.08)  LDD2237  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [5]
NAIA_5
 Probe Info  		N.A.  LDD2224  [6]
IPM
 Probe Info  		N.A.  LDD0005  [7]
OSF
 Probe Info  		N.A.  LDD0029  [8]
SF
 Probe Info  		Y185(0.00); Y44(0.00); Y23(0.00)  LDD0028  [8]
VSF
 Probe Info  		N.A.  LDD0007  [7]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0116  HHS-0101 DM93 Y23(0.38); Y44(1.05)  LDD0264  [3]
 LDCM0117  HHS-0201 DM93 Y23(0.51); Y44(0.91)  LDD0265  [3]
 LDCM0118  HHS-0301 DM93 Y23(0.50); Y44(0.95)  LDD0266  [3]
 LDCM0119  HHS-0401 DM93 Y23(0.41); Y44(1.08)  LDD0267  [3]
 LDCM0120  HHS-0701 DM93 Y23(0.33); Y44(0.91)  LDD0268  [3]
 LDCM0123  JWB131 DM93 Y44(0.96)  LDD0285  [2]
 LDCM0124  JWB142 DM93 Y44(0.65)  LDD0286  [2]
 LDCM0125  JWB146 DM93 Y44(1.21)  LDD0287  [2]
 LDCM0126  JWB150 DM93 Y44(2.63)  LDD0288  [2]
 LDCM0127  JWB152 DM93 Y44(1.60)  LDD0289  [2]
 LDCM0128  JWB198 DM93 Y44(0.94)  LDD0290  [2]
 LDCM0129  JWB202 DM93 Y44(0.36)  LDD0291  [2]
 LDCM0130  JWB211 DM93 Y44(0.91)  LDD0292  [2]
 LDCM0022  KB02 CCK-81 C141(2.32)  LDD2297  [1]
 LDCM0023  KB03 CCK-81 C141(1.82)  LDD2714  [1]
 LDCM0024  KB05 MKN45 C141(1.29)  LDD3332  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SRSF protein kinase 1 (SRPK1) CMGC Ser/Thr protein kinase family Q96SB4
SRSF protein kinase 2 (SRPK2) CMGC Ser/Thr protein kinase family P78362
Dual specificity protein kinase CLK3 (CLK3) CMGC Ser/Thr protein kinase family P49761
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Keratin-associated protein 12-2 (KRTAP12-2) KRTAP type 12 family P59991
Serine/arginine-rich splicing factor 3 (SRSF3) Splicing factor SR family P84103
Transformer-2 protein homolog beta (TRA2B) Splicing factor SR family P62995

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
5 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
9 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004