Details of the Target

General Information of Target

Target ID LDTP09721
Target Name DnaJ homolog subfamily C member 9 (DNAJC9)
Gene Name DNAJC9
Gene ID 23234
Synonyms
DnaJ homolog subfamily C member 9; HDJC9; DnaJ protein SB73
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGLLDLCEEVFGTADLYRVLGVRREASDGEVRRGYHKVSLQVHPDRVGEGDKEDATRRFQ
ILGKVYSVLSDREQRAVYDEQGTVDEDSPVLTQDRDWEAYWRLLFKKISLEDIQAFEKTY
KGSEEELADIKQAYLDFKGDMDQIMESVLCVQYTEEPRIRNIIQQAIDAGEVPSYNAFVK
ESKQKMNARKRRAQEEAKEAEMSRKELGLDEGVDSLKAAIQSRQKDRQKEMDNFLAQMEA
KYCKSSKGGGKKSALKKEKK
Target Bioclass
Other
Subcellular location
Nucleus
Function
Acts as a dual histone chaperone and heat shock co-chaperone. As a histone chaperone, forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers; and may thereby assist MCM2 in histone H3-H4 heterodimer recognition and facilitate the assembly of histones into nucleosomes. May also act as a histone co-chaperone together with TONSL. May recruit histone chaperones ASF1A, NASP and SPT2 to histone H3-H4 heterodimers. Also plays a role as co-chaperone of the HSP70 family of molecular chaperone proteins, such as HSPA1A, HSPA1B and HSPA8. As a co-chaperone, may play a role in the recruitment of HSP70-type molecular chaperone machinery to histone H3-H4 substrates, thereby maintaining the histone structural integrity. Exhibits activity to assemble histones onto DNA in vitro.
Uniprot ID
Q8WXX5
Ensemble ID
ENST00000372950.6
HGNC ID
HGNC:19123

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
CY-1
 Probe Info  		100.00  LDD0243  [1]
STPyne
 Probe Info  		K107(9.02); K217(10.00); K244(10.00)  LDD0277  [2]
AZ-9
 Probe Info  		E239(10.00)  LDD2209  [3]
OPA-S-S-alkyne
 Probe Info  		K198(2.94)  LDD3494  [4]
Probe 1
 Probe Info  		Y78(14.12)  LDD3495  [5]
m-APA
 Probe Info  		15.00  LDD0403  [6]
DBIA
 Probe Info  		C7(0.97)  LDD3332  [7]
JZ128-DTB
 Probe Info  		N.A.  LDD0462  [8]
N1
 Probe Info  		N.A.  LDD0245  [1]
4-Iodoacetamidophenylacetylene
 Probe Info  		C7(0.00); C150(0.00)  LDD0038  [9]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [10]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [11]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [11]
NHS
 Probe Info  		N.A.  LDD0010  [12]
AOyne
 Probe Info  		12.20  LDD0443  [13]
NAIA_5
 Probe Info  		N.A.  LDD2223  [14]
PAL-AfBPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C278
 Probe Info  		72.00  LDD1948  [15]
FFF probe11
 Probe Info  		5.79  LDD0472  [16]
FFF probe13
 Probe Info  		12.32  LDD0475  [16]
FFF probe2
 Probe Info  		5.84  LDD0463  [16]
FFF probe3
 Probe Info  		8.38  LDD0465  [16]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C150(0.85)  LDD1507  [17]
 LDCM0215  AC10 HEK-293T C150(0.98)  LDD1508  [17]
 LDCM0226  AC11 HEK-293T C150(1.03)  LDD1509  [17]
 LDCM0259  AC14 HEK-293T C150(0.95)  LDD1512  [17]
 LDCM0270  AC15 HEK-293T C150(0.84)  LDD1513  [17]
 LDCM0276  AC17 HEK-293T C150(0.92)  LDD1515  [17]
 LDCM0277  AC18 HEK-293T C150(0.98)  LDD1516  [17]
 LDCM0278  AC19 HEK-293T C150(0.76)  LDD1517  [17]
 LDCM0279  AC2 HEK-293T C150(0.85)  LDD1518  [17]
 LDCM0282  AC22 HEK-293T C150(1.15)  LDD1521  [17]
 LDCM0283  AC23 HEK-293T C150(0.73)  LDD1522  [17]
 LDCM0284  AC24 HEK-293T C150(1.20)  LDD1523  [17]
 LDCM0285  AC25 HEK-293T C150(0.79)  LDD1524  [17]
 LDCM0286  AC26 HEK-293T C150(0.80)  LDD1525  [17]
 LDCM0287  AC27 HEK-293T C150(0.93)  LDD1526  [17]
 LDCM0290  AC3 HEK-293T C150(1.26)  LDD1529  [17]
 LDCM0291  AC30 HEK-293T C150(1.53)  LDD1530  [17]
 LDCM0292  AC31 HEK-293T C150(0.74)  LDD1531  [17]
 LDCM0293  AC32 HEK-293T C150(0.87)  LDD1532  [17]
 LDCM0294  AC33 HEK-293T C150(0.91)  LDD1533  [17]
 LDCM0295  AC34 HEK-293T C150(0.80)  LDD1534  [17]
 LDCM0296  AC35 HEK-293T C150(1.01)  LDD1535  [17]
 LDCM0299  AC38 HEK-293T C150(1.05)  LDD1538  [17]
 LDCM0300  AC39 HEK-293T C150(0.94)  LDD1539  [17]
 LDCM0302  AC40 HEK-293T C150(0.98)  LDD1541  [17]
 LDCM0303  AC41 HEK-293T C150(1.09)  LDD1542  [17]
 LDCM0304  AC42 HEK-293T C150(0.95)  LDD1543  [17]
 LDCM0305  AC43 HEK-293T C150(0.95)  LDD1544  [17]
 LDCM0308  AC46 HEK-293T C150(1.10)  LDD1547  [17]
 LDCM0309  AC47 HEK-293T C150(0.67)  LDD1548  [17]
 LDCM0310  AC48 HEK-293T C150(1.17)  LDD1549  [17]
 LDCM0311  AC49 HEK-293T C150(0.90)  LDD1550  [17]
 LDCM0313  AC50 HEK-293T C150(0.82)  LDD1552  [17]
 LDCM0314  AC51 HEK-293T C150(0.94)  LDD1553  [17]
 LDCM0317  AC54 HEK-293T C150(0.99)  LDD1556  [17]
 LDCM0318  AC55 HEK-293T C150(0.68)  LDD1557  [17]
 LDCM0319  AC56 HEK-293T C150(0.94)  LDD1558  [17]
 LDCM0320  AC57 HEK-293T C150(0.83)  LDD1559  [17]
 LDCM0321  AC58 HEK-293T C150(0.83)  LDD1560  [17]
 LDCM0322  AC59 HEK-293T C150(1.02)  LDD1561  [17]
 LDCM0323  AC6 HEK-293T C150(1.18)  LDD1562  [17]
 LDCM0326  AC62 HEK-293T C150(1.24)  LDD1565  [17]
 LDCM0327  AC63 HEK-293T C150(0.83)  LDD1566  [17]
 LDCM0328  AC64 HEK-293T C150(0.89)  LDD1567  [17]
 LDCM0334  AC7 HEK-293T C150(0.90)  LDD1568  [17]
 LDCM0345  AC8 HEK-293T C150(1.05)  LDD1569  [17]
 LDCM0356  AKOS034007680 HEK-293T C150(0.46)  LDD1570  [17]
 LDCM0275  AKOS034007705 HEK-293T C150(0.92)  LDD1514  [17]
 LDCM0156  Aniline NCI-H1299 15.00  LDD0403  [6]
 LDCM0367  CL1 HEK-293T C150(1.13)  LDD1571  [17]
 LDCM0368  CL10 HEK-293T C150(0.75)  LDD1572  [17]
 LDCM0370  CL101 HEK-293T C150(1.05)  LDD1574  [17]
 LDCM0371  CL102 HEK-293T C150(1.12)  LDD1575  [17]
 LDCM0372  CL103 HEK-293T C150(0.98)  LDD1576  [17]
 LDCM0374  CL105 HEK-293T C150(1.02)  LDD1578  [17]
 LDCM0375  CL106 HEK-293T C150(1.19)  LDD1579  [17]
 LDCM0376  CL107 HEK-293T C150(1.09)  LDD1580  [17]
 LDCM0378  CL109 HEK-293T C150(1.00)  LDD1582  [17]
 LDCM0379  CL11 HEK-293T C150(0.64)  LDD1583  [17]
 LDCM0380  CL110 HEK-293T C150(1.12)  LDD1584  [17]
 LDCM0381  CL111 HEK-293T C150(1.03)  LDD1585  [17]
 LDCM0383  CL113 HEK-293T C150(1.15)  LDD1587  [17]
 LDCM0384  CL114 HEK-293T C150(0.92)  LDD1588  [17]
 LDCM0385  CL115 HEK-293T C150(0.91)  LDD1589  [17]
 LDCM0387  CL117 HEK-293T C150(1.14)  LDD1591  [17]
 LDCM0388  CL118 HEK-293T C150(1.20)  LDD1592  [17]
 LDCM0389  CL119 HEK-293T C150(1.12)  LDD1593  [17]
 LDCM0390  CL12 HEK-293T C150(1.01)  LDD1594  [17]
 LDCM0392  CL121 HEK-293T C150(1.07)  LDD1596  [17]
 LDCM0393  CL122 HEK-293T C150(0.80)  LDD1597  [17]
 LDCM0394  CL123 HEK-293T C150(0.86)  LDD1598  [17]
 LDCM0396  CL125 HEK-293T C150(1.04)  LDD1600  [17]
 LDCM0397  CL126 HEK-293T C150(0.41)  LDD1601  [17]
 LDCM0398  CL127 HEK-293T C150(0.90)  LDD1602  [17]
 LDCM0400  CL13 HEK-293T C150(1.10)  LDD1604  [17]
 LDCM0401  CL14 HEK-293T C150(1.30)  LDD1605  [17]
 LDCM0402  CL15 HEK-293T C150(0.72)  LDD1606  [17]
 LDCM0404  CL17 HEK-293T C150(0.68)  LDD1608  [17]
 LDCM0405  CL18 HEK-293T C150(0.88)  LDD1609  [17]
 LDCM0406  CL19 HEK-293T C150(1.07)  LDD1610  [17]
 LDCM0407  CL2 HEK-293T C150(1.33)  LDD1611  [17]
 LDCM0410  CL22 HEK-293T C150(0.81)  LDD1614  [17]
 LDCM0411  CL23 HEK-293T C150(0.84)  LDD1615  [17]
 LDCM0412  CL24 HEK-293T C150(1.04)  LDD1616  [17]
 LDCM0413  CL25 HEK-293T C150(0.97)  LDD1617  [17]
 LDCM0414  CL26 HEK-293T C150(1.25)  LDD1618  [17]
 LDCM0415  CL27 HEK-293T C150(0.84)  LDD1619  [17]
 LDCM0417  CL29 HEK-293T C150(0.87)  LDD1621  [17]
 LDCM0418  CL3 HEK-293T C150(0.99)  LDD1622  [17]
 LDCM0419  CL30 HEK-293T C150(1.00)  LDD1623  [17]
 LDCM0420  CL31 HEK-293T C150(1.15)  LDD1624  [17]
 LDCM0423  CL34 HEK-293T C150(0.89)  LDD1627  [17]
 LDCM0424  CL35 HEK-293T C150(0.79)  LDD1628  [17]
 LDCM0425  CL36 HEK-293T C150(0.74)  LDD1629  [17]
 LDCM0426  CL37 HEK-293T C150(1.27)  LDD1630  [17]
 LDCM0428  CL39 HEK-293T C150(0.85)  LDD1632  [17]
 LDCM0431  CL41 HEK-293T C150(1.04)  LDD1635  [17]
 LDCM0432  CL42 HEK-293T C150(0.92)  LDD1636  [17]
 LDCM0433  CL43 HEK-293T C150(1.19)  LDD1637  [17]
 LDCM0436  CL46 HEK-293T C150(1.00)  LDD1640  [17]
 LDCM0437  CL47 HEK-293T C150(0.97)  LDD1641  [17]
 LDCM0438  CL48 HEK-293T C150(0.98)  LDD1642  [17]
 LDCM0439  CL49 HEK-293T C150(1.24)  LDD1643  [17]
 LDCM0440  CL5 HEK-293T C150(1.06)  LDD1644  [17]
 LDCM0441  CL50 HEK-293T C150(1.09)  LDD1645  [17]
 LDCM0444  CL53 HEK-293T C150(0.79)  LDD1647  [17]
 LDCM0445  CL54 HEK-293T C150(0.97)  LDD1648  [17]
 LDCM0446  CL55 HEK-293T C150(1.07)  LDD1649  [17]
 LDCM0449  CL58 HEK-293T C150(0.87)  LDD1652  [17]
 LDCM0450  CL59 HEK-293T C150(0.82)  LDD1653  [17]
 LDCM0451  CL6 HEK-293T C150(0.89)  LDD1654  [17]
 LDCM0452  CL60 HEK-293T C150(0.93)  LDD1655  [17]
 LDCM0453  CL61 HEK-293T C150(0.99)  LDD1656  [17]
 LDCM0454  CL62 HEK-293T C150(1.32)  LDD1657  [17]
 LDCM0455  CL63 HEK-293T C150(1.03)  LDD1658  [17]
 LDCM0457  CL65 HEK-293T C150(1.13)  LDD1660  [17]
 LDCM0458  CL66 HEK-293T C150(1.00)  LDD1661  [17]
 LDCM0459  CL67 HEK-293T C150(1.14)  LDD1662  [17]
 LDCM0462  CL7 HEK-293T C150(1.14)  LDD1665  [17]
 LDCM0463  CL70 HEK-293T C150(1.20)  LDD1666  [17]
 LDCM0464  CL71 HEK-293T C150(0.68)  LDD1667  [17]
 LDCM0465  CL72 HEK-293T C150(0.85)  LDD1668  [17]
 LDCM0466  CL73 HEK-293T C150(0.90)  LDD1669  [17]
 LDCM0467  CL74 HEK-293T C150(0.89)  LDD1670  [17]
 LDCM0470  CL77 HEK-293T C150(0.86)  LDD1673  [17]
 LDCM0471  CL78 HEK-293T C150(0.89)  LDD1674  [17]
 LDCM0472  CL79 HEK-293T C150(1.15)  LDD1675  [17]
 LDCM0476  CL82 HEK-293T C150(1.77)  LDD1679  [17]
 LDCM0477  CL83 HEK-293T C150(0.89)  LDD1680  [17]
 LDCM0478  CL84 HEK-293T C150(0.77)  LDD1681  [17]
 LDCM0479  CL85 HEK-293T C150(1.16)  LDD1682  [17]
 LDCM0480  CL86 HEK-293T C150(1.55)  LDD1683  [17]
 LDCM0481  CL87 HEK-293T C150(1.03)  LDD1684  [17]
 LDCM0483  CL89 HEK-293T C150(0.84)  LDD1686  [17]
 LDCM0485  CL90 HEK-293T C150(0.82)  LDD1688  [17]
 LDCM0486  CL91 HEK-293T C150(1.07)  LDD1689  [17]
 LDCM0489  CL94 HEK-293T C150(1.02)  LDD1692  [17]
 LDCM0490  CL95 HEK-293T C150(0.45)  LDD1693  [17]
 LDCM0491  CL96 HEK-293T C150(0.85)  LDD1694  [17]
 LDCM0492  CL97 HEK-293T C150(0.99)  LDD1695  [17]
 LDCM0493  CL98 HEK-293T C150(1.06)  LDD1696  [17]
 LDCM0494  CL99 HEK-293T C150(0.81)  LDD1697  [17]
 LDCM0495  E2913 HEK-293T C150(0.91)  LDD1698  [17]
 LDCM0468  Fragment33 HEK-293T C150(0.88)  LDD1671  [17]
 LDCM0427  Fragment51 HEK-293T C150(1.13)  LDD1631  [17]
 LDCM0179  JZ128 PC-3 N.A.  LDD0462  [8]
 LDCM0022  KB02 HEK-293T C150(1.09)  LDD1492  [17]
 LDCM0023  KB03 HEK-293T C150(1.10)  LDD1497  [17]
 LDCM0024  KB05 MKN45 C7(0.97)  LDD3332  [7]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone H3.1 (H3C1; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3C10; H3C11; H3C12) Histone H3 family P68431
Histone H3.3C (H3-5) Histone H3 family Q6NXT2
MORN repeat-containing protein 4 (MORN4) . Q8NDC4

References

1 Cyclopropenone, Cyclopropeniminium Ion, and Cyclopropenethione as Novel Electrophilic Warheads for Potential Target Discovery of Triple-Negative Breast Cancer. J Med Chem. 2023 Feb 23;66(4):2851-2864. doi: 10.1021/acs.jmedchem.2c01889. Epub 2023 Feb 10.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
4 A chemical proteomics approach for global mapping of functional lysines on cell surface of living cell. Nat Commun. 2024 Apr 8;15(1):2997. doi: 10.1038/s41467-024-47033-w.
Mass spectrometry data entry: PXD042888
5 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
6 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
8 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
9 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
10 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
11 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
12 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
13 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
14 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
15 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
16 Ligand and Target Discovery by Fragment-Based Screening in Human Cells. Cell. 2017 Jan 26;168(3):527-541.e29. doi: 10.1016/j.cell.2016.12.029. Epub 2017 Jan 19.
17 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402