Details of the Target

General Information of Target

Target ID LDTP09305
Target Name Tetratricopeptide repeat protein 8 (TTC8)
Gene Name TTC8
Gene ID 123016
Synonyms
BBS8; Tetratricopeptide repeat protein 8; TPR repeat protein 8; Bardet-Biedl syndrome 8 protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSSEMEPLLLAWSYFRRRKFQLCADLCTQMLEKSPYDQEPDPELPVHQAAWILKARALTE
MVYIDEIDVDQEGIAEMMLDENAIAQVPRPGTSLKLPGTNQTGGPSQAVRPITQAGRPIT
GFLRPSTQSGRPGTMEQAIRTPRTAYTARPITSSSGRFVRLGTASMLTSPDGPFINLSRL
NLTKYSQKPKLAKALFEYIFHHENDVKTIHLEDVVLHLGIYPFLLRNKNHIEKNALDLAA
LSTEHSQYKDWWWKVQIGKCYYRLGMYREAEKQFKSALKQQEMVDTFLYLAKVYVSLDQP
VTALNLFKQGLDKFPGEVTLLCGIARIYEEMNNMSSAAEYYKEVLKQDNTHVEAIACIGS
NHFYSDQPEIALRFYRRLLQMGIYNGQLFNNLGLCCFYAQQYDMTLTSFERALSLAENEE
EAADVWYNLGHVAVGIGDTNLAHQCFRLALVNNNNHAEAYNNLAVLEMRKGHVEQARALL
QTASSLAPHMYEPHFNFATISDKIGDLQRSYVAAQKSEAAFPDHVDTQHLIKQLRQHFAM
L
Target Bioclass
Transporter and channel
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization.
Uniprot ID
Q8TAM2
Ensemble ID
ENST00000354441.10
HGNC ID
HGNC:20087

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C322(0.83)  LDD0533  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C322(0.99)  LDD1507  [2]
 LDCM0216  AC100 HCT 116 C322(0.83)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C322(1.00)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C322(0.90)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C322(1.05)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C322(0.82)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C322(0.93)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C322(0.95)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C322(0.80)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C322(0.89)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C322(0.90)  LDD0542  [1]
 LDCM0227  AC110 HCT 116 C322(0.91)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C322(0.93)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C322(0.85)  LDD0546  [1]
 LDCM0276  AC17 HEK-293T C322(1.17)  LDD1515  [2]
 LDCM0281  AC21 HEK-293T C322(0.99)  LDD1520  [2]
 LDCM0284  AC24 HEK-293T C322(1.00)  LDD1523  [2]
 LDCM0285  AC25 HEK-293T C322(0.89)  LDD1524  [2]
 LDCM0289  AC29 HEK-293T C322(1.02)  LDD1528  [2]
 LDCM0293  AC32 HEK-293T C322(1.05)  LDD1532  [2]
 LDCM0294  AC33 HEK-293T C322(1.04)  LDD1533  [2]
 LDCM0298  AC37 HEK-293T C322(1.08)  LDD1537  [2]
 LDCM0302  AC40 HEK-293T C322(0.97)  LDD1541  [2]
 LDCM0303  AC41 HEK-293T C322(1.06)  LDD1542  [2]
 LDCM0307  AC45 HEK-293T C322(1.00)  LDD1546  [2]
 LDCM0310  AC48 HEK-293T C322(1.01)  LDD1549  [2]
 LDCM0311  AC49 HEK-293T C322(1.07)  LDD1550  [2]
 LDCM0312  AC5 HEK-293T C322(1.15)  LDD1551  [2]
 LDCM0316  AC53 HEK-293T C322(1.08)  LDD1555  [2]
 LDCM0319  AC56 HEK-293T C322(0.97)  LDD1558  [2]
 LDCM0320  AC57 HCT 116 C322(1.33)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C322(1.32)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C322(1.15)  LDD0639  [1]
 LDCM0324  AC60 HCT 116 C322(1.34)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C322(1.40)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C322(1.56)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C322(1.30)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C322(1.53)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C322(1.63)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C322(1.80)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C322(1.62)  LDD0648  [1]
 LDCM0345  AC8 HEK-293T C322(1.06)  LDD1569  [2]
 LDCM0365  AC98 HCT 116 C322(0.92)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C322(1.10)  LDD0683  [1]
 LDCM0248  AKOS034007472 HEK-293T C322(1.08)  LDD1511  [2]
 LDCM0356  AKOS034007680 HEK-293T C322(1.07)  LDD1570  [2]
 LDCM0275  AKOS034007705 HEK-293T C322(1.08)  LDD1514  [2]
 LDCM0367  CL1 HEK-293T C322(1.66)  LDD1571  [2]
 LDCM0369  CL100 HEK-293T C322(1.17)  LDD1573  [2]
 LDCM0370  CL101 HEK-293T C322(1.24)  LDD1574  [2]
 LDCM0371  CL102 HEK-293T C322(0.95)  LDD1575  [2]
 LDCM0372  CL103 HEK-293T C322(1.02)  LDD1576  [2]
 LDCM0373  CL104 HEK-293T C322(0.88)  LDD1577  [2]
 LDCM0374  CL105 HEK-293T C322(1.02)  LDD1578  [2]
 LDCM0375  CL106 HEK-293T C322(0.80)  LDD1579  [2]
 LDCM0376  CL107 HEK-293T C322(0.99)  LDD1580  [2]
 LDCM0377  CL108 HEK-293T C322(1.08)  LDD1581  [2]
 LDCM0378  CL109 HEK-293T C322(1.46)  LDD1582  [2]
 LDCM0380  CL110 HEK-293T C322(0.87)  LDD1584  [2]
 LDCM0381  CL111 HEK-293T C322(1.00)  LDD1585  [2]
 LDCM0382  CL112 HEK-293T C322(0.83)  LDD1586  [2]
 LDCM0383  CL113 HEK-293T C322(1.18)  LDD1587  [2]
 LDCM0384  CL114 HEK-293T C322(1.18)  LDD1588  [2]
 LDCM0385  CL115 HEK-293T C322(1.06)  LDD1589  [2]
 LDCM0386  CL116 HEK-293T C322(1.21)  LDD1590  [2]
 LDCM0387  CL117 HEK-293T C322(1.16)  LDD1591  [2]
 LDCM0388  CL118 HEK-293T C322(1.04)  LDD1592  [2]
 LDCM0389  CL119 HEK-293T C322(1.03)  LDD1593  [2]
 LDCM0390  CL12 HEK-293T C322(1.24)  LDD1594  [2]
 LDCM0391  CL120 HEK-293T C322(1.11)  LDD1595  [2]
 LDCM0392  CL121 HEK-293T C322(1.17)  LDD1596  [2]
 LDCM0393  CL122 HEK-293T C322(1.37)  LDD1597  [2]
 LDCM0394  CL123 HEK-293T C322(1.26)  LDD1598  [2]
 LDCM0395  CL124 HEK-293T C322(1.19)  LDD1599  [2]
 LDCM0396  CL125 HCT 116 C322(0.94)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C322(1.49)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C322(1.35)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C322(1.62)  LDD0716  [1]
 LDCM0400  CL13 HEK-293T C322(1.49)  LDD1604  [2]
 LDCM0401  CL14 HEK-293T C322(0.88)  LDD1605  [2]
 LDCM0402  CL15 HEK-293T C322(0.92)  LDD1606  [2]
 LDCM0403  CL16 HCT 116 C322(1.10)  LDD0720  [1]
 LDCM0404  CL17 HEK-293T C322(1.17)  LDD1608  [2]
 LDCM0407  CL2 HEK-293T C322(0.89)  LDD1611  [2]
 LDCM0409  CL21 HEK-293T C322(0.94)  LDD1613  [2]
 LDCM0412  CL24 HEK-293T C322(1.11)  LDD1616  [2]
 LDCM0413  CL25 HEK-293T C322(1.18)  LDD1617  [2]
 LDCM0414  CL26 HEK-293T C322(0.88)  LDD1618  [2]
 LDCM0415  CL27 HEK-293T C322(0.94)  LDD1619  [2]
 LDCM0416  CL28 HEK-293T C322(1.07)  LDD1620  [2]
 LDCM0417  CL29 HEK-293T C322(1.14)  LDD1621  [2]
 LDCM0418  CL3 HEK-293T C322(1.02)  LDD1622  [2]
 LDCM0421  CL32 HCT 116 C322(1.04)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C322(2.05)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C322(1.25)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C322(0.92)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C322(1.31)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C322(1.13)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C322(1.05)  LDD0745  [1]
 LDCM0429  CL4 HEK-293T C322(1.09)  LDD1633  [2]
 LDCM0430  CL40 HCT 116 C322(1.08)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C322(1.11)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C322(1.07)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C322(1.12)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C322(1.06)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C322(1.08)  LDD0752  [1]
 LDCM0436  CL46 HCT 116 C322(1.21)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C322(1.53)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C322(0.88)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C322(1.23)  LDD0756  [1]
 LDCM0440  CL5 HEK-293T C322(1.01)  LDD1644  [2]
 LDCM0441  CL50 HCT 116 C322(0.96)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C322(0.87)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C322(0.98)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C322(0.88)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C322(1.18)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C322(0.94)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C322(1.73)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C322(1.53)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C322(0.84)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C322(1.01)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C322(1.64)  LDD0769  [1]
 LDCM0453  CL61 HEK-293T C322(1.08)  LDD1656  [2]
 LDCM0454  CL62 HEK-293T C322(0.89)  LDD1657  [2]
 LDCM0455  CL63 HEK-293T C322(1.13)  LDD1658  [2]
 LDCM0456  CL64 HEK-293T C322(1.20)  LDD1659  [2]
 LDCM0457  CL65 HEK-293T C322(0.99)  LDD1660  [2]
 LDCM0461  CL69 HEK-293T C322(1.02)  LDD1664  [2]
 LDCM0465  CL72 HEK-293T C322(1.12)  LDD1668  [2]
 LDCM0466  CL73 HEK-293T C322(1.24)  LDD1669  [2]
 LDCM0467  CL74 HEK-293T C322(1.35)  LDD1670  [2]
 LDCM0469  CL76 HEK-293T C322(1.08)  LDD1672  [2]
 LDCM0470  CL77 HEK-293T C322(1.05)  LDD1673  [2]
 LDCM0475  CL81 HEK-293T C322(0.96)  LDD1678  [2]
 LDCM0478  CL84 HEK-293T C322(1.08)  LDD1681  [2]
 LDCM0479  CL85 HEK-293T C322(1.32)  LDD1682  [2]
 LDCM0480  CL86 HEK-293T C322(1.18)  LDD1683  [2]
 LDCM0481  CL87 HEK-293T C322(1.08)  LDD1684  [2]
 LDCM0482  CL88 HEK-293T C322(1.22)  LDD1685  [2]
 LDCM0483  CL89 HEK-293T C322(1.14)  LDD1686  [2]
 LDCM0484  CL9 HEK-293T C322(0.98)  LDD1687  [2]
 LDCM0488  CL93 HEK-293T C322(0.99)  LDD1691  [2]
 LDCM0491  CL96 HEK-293T C322(1.38)  LDD1694  [2]
 LDCM0492  CL97 HEK-293T C322(1.32)  LDD1695  [2]
 LDCM0493  CL98 HEK-293T C322(1.14)  LDD1696  [2]
 LDCM0494  CL99 HEK-293T C322(1.32)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C322(1.19)  LDD1698  [2]
 LDCM0468  Fragment33 HEK-293T C322(1.01)  LDD1671  [2]
 LDCM0427  Fragment51 HCT 116 C322(1.10)  LDD0744  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein PTHB1 (BBS9) . Q3SYG4
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
IQ calmodulin-binding motif-containing protein 1 (IQCB1) . Q15051

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402