Details of the Target

General Information of Target

Target ID LDTP09124
Target Name Terminal nucleotidyltransferase 4B (TENT4B)
Gene Name TENT4B
Gene ID 64282
Synonyms
GLD4; PAPD5; TRF4-2; TUT3; Terminal nucleotidyltransferase 4B; Non-canonical poly(A) RNA polymerase PAPD5; EC 2.7.7.19; PAP-associated domain-containing protein 5; Terminal guanylyltransferase; EC 2.7.7.-; Terminal uridylyltransferase 3; TUTase 3; Topoisomerase-related function protein 4-2; TRF4-2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MYRSGERLLGSHALPAEQRDFLPLETTNNNNNHHQPGAWARRAGSSASSPPSASSSPHPS
AAVPAADPADSASGSSNKRKRDNKASGGRAAGGGRADGGGVVYSGTPWKRRNYNQGVVGL
HEEISDFYEYMSPRPEEEKMRMEVVNRIESVIKELWPSADVQIFGSFKTGLYLPTSDIDL
VVFGKWENLPLWTLEEALRKHKVADEDSVKVLDKATVPIIKLTDSFTEVKVDISFNVQNG
VRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIGSYSLFLMAVSFLQLHPRE
DACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAKDEVQKNMLDGYRPSMLYI
EDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYYPNNETESILGRIIRVTDE
VATYRDWISKQWGLKNRPEPSCNGPVSSSSATQSSSSDVDSDATPCKTPKQLLCRPSTGN
RVGSQDVSLESSQAVGKMQSTQTTNTSNSTNKSQHGSARLFRSSSKGFQGTTQTSHGSLM
TNKQHQGKSNNQYYHGKKRKHKRDAPLSDLCR
Target Type
Clinical trial
Target Bioclass
Enzyme
Family
DNA polymerase type-B-like family
Subcellular location
Nucleus
Function
Terminal nucleotidyltransferase that catalyzes preferentially the transfer of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1. Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion. Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation. Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation.
TTD ID
T63241
Uniprot ID
Q8NDF8
DrugMap ID
TTB5R6M
Ensemble ID
ENST00000436909.8
HGNC ID
HGNC:30758
ChEMBL ID
CHEMBL4680036

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
AHL-Pu-1
 Probe Info  		C571(3.26)  LDD0169  [1]
DBIA
 Probe Info  		C442(1.44); C466(1.44)  LDD0078  [2]
TFBX
 Probe Info  		N.A.  LDD0027  [3]
IPM
 Probe Info  		C474(0.00); C571(0.00)  LDD0147  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C571(3.26)  LDD0169  [1]
 LDCM0215  AC10 HCT 116 C442(0.93); C466(0.93)  LDD0532  [2]
 LDCM0216  AC100 HEK-293T C442(1.04); C466(1.04)  LDD0814  [2]
 LDCM0217  AC101 HEK-293T C442(1.09); C466(1.09)  LDD0815  [2]
 LDCM0218  AC102 HEK-293T C442(1.06); C466(1.06)  LDD0816  [2]
 LDCM0219  AC103 HEK-293T C442(0.95); C466(0.95)  LDD0817  [2]
 LDCM0220  AC104 HEK-293T C442(1.07); C466(1.07)  LDD0818  [2]
 LDCM0221  AC105 HEK-293T C442(0.93); C466(0.93)  LDD0819  [2]
 LDCM0222  AC106 HEK-293T C442(1.04); C466(1.04)  LDD0820  [2]
 LDCM0223  AC107 HEK-293T C442(1.18); C466(1.18)  LDD0821  [2]
 LDCM0224  AC108 HEK-293T C442(1.35); C466(1.35)  LDD0822  [2]
 LDCM0225  AC109 HEK-293T C442(1.22); C466(1.22)  LDD0823  [2]
 LDCM0226  AC11 HCT 116 C442(1.82); C466(1.82)  LDD0543  [2]
 LDCM0227  AC110 HEK-293T C442(1.11); C466(1.11)  LDD0825  [2]
 LDCM0228  AC111 HEK-293T C442(1.12); C466(1.12)  LDD0826  [2]
 LDCM0229  AC112 HEK-293T C442(1.05); C466(1.05)  LDD0827  [2]
 LDCM0237  AC12 HCT 116 C442(1.39); C466(1.39)  LDD0554  [2]
 LDCM0259  AC14 HCT 116 C442(1.33); C466(1.33)  LDD0576  [2]
 LDCM0263  AC143 HCT 116 C442(1.30); C466(1.30)  LDD0580  [2]
 LDCM0264  AC144 HCT 116 C442(1.32); C466(1.32)  LDD0581  [2]
 LDCM0265  AC145 HCT 116 C442(1.16); C466(1.16)  LDD0582  [2]
 LDCM0266  AC146 HCT 116 C442(1.19); C466(1.19)  LDD0583  [2]
 LDCM0267  AC147 HCT 116 C442(1.13); C466(1.13)  LDD0584  [2]
 LDCM0268  AC148 HCT 116 C442(2.36); C466(2.36)  LDD0585  [2]
 LDCM0269  AC149 HCT 116 C442(1.60); C466(1.60)  LDD0586  [2]
 LDCM0270  AC15 HCT 116 C442(1.32); C466(1.32)  LDD0587  [2]
 LDCM0271  AC150 HCT 116 C442(0.91); C466(0.91)  LDD0588  [2]
 LDCM0272  AC151 HCT 116 C442(1.06); C466(1.06)  LDD0589  [2]
 LDCM0273  AC152 HCT 116 C442(1.42); C466(1.42)  LDD0590  [2]
 LDCM0274  AC153 HCT 116 C442(2.16); C466(2.16)  LDD0591  [2]
 LDCM0621  AC154 HCT 116 C442(1.25); C466(1.25)  LDD2158  [2]
 LDCM0622  AC155 HCT 116 C442(1.96); C466(1.96)  LDD2159  [2]
 LDCM0623  AC156 HCT 116 C442(1.20); C466(1.20)  LDD2160  [2]
 LDCM0624  AC157 HCT 116 C442(0.85); C466(0.85)  LDD2161  [2]
 LDCM0276  AC17 HCT 116 C442(1.31); C466(1.31)  LDD0593  [2]
 LDCM0277  AC18 HCT 116 C442(1.40); C466(1.40)  LDD0594  [2]
 LDCM0278  AC19 HCT 116 C442(1.33); C466(1.33)  LDD0595  [2]
 LDCM0280  AC20 HCT 116 C442(1.33); C466(1.33)  LDD0597  [2]
 LDCM0281  AC21 HCT 116 C442(1.23); C466(1.23)  LDD0598  [2]
 LDCM0282  AC22 HCT 116 C442(1.00); C466(1.00)  LDD0599  [2]
 LDCM0283  AC23 HCT 116 C442(1.31); C466(1.31)  LDD0600  [2]
 LDCM0284  AC24 HCT 116 C442(1.28); C466(1.28)  LDD0601  [2]
 LDCM0285  AC25 HCT 116 C442(0.81); C466(0.81)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C442(0.98); C466(0.98)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C442(0.86); C466(0.86)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C442(0.80); C466(0.80)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C442(0.70); C466(0.70)  LDD0606  [2]
 LDCM0291  AC30 HCT 116 C442(0.97); C466(0.97)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C442(0.98); C466(0.98)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C442(1.13); C466(1.13)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C442(0.88); C466(0.88)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C442(1.12); C466(1.12)  LDD0612  [2]
 LDCM0296  AC35 HCT 116 C442(1.04); C466(1.04)  LDD0613  [2]
 LDCM0297  AC36 HCT 116 C442(1.05); C466(1.05)  LDD0614  [2]
 LDCM0298  AC37 HCT 116 C442(1.05); C466(1.05)  LDD0615  [2]
 LDCM0299  AC38 HCT 116 C442(1.05); C466(1.05)  LDD0616  [2]
 LDCM0300  AC39 HCT 116 C442(1.24); C466(1.24)  LDD0617  [2]
 LDCM0302  AC40 HCT 116 C442(1.52); C466(1.52)  LDD0619  [2]
 LDCM0303  AC41 HCT 116 C442(1.14); C466(1.14)  LDD0620  [2]
 LDCM0304  AC42 HCT 116 C442(1.18); C466(1.18)  LDD0621  [2]
 LDCM0305  AC43 HCT 116 C442(1.04); C466(1.04)  LDD0622  [2]
 LDCM0306  AC44 HCT 116 C442(1.27); C466(1.27)  LDD0623  [2]
 LDCM0307  AC45 HCT 116 C442(1.40); C466(1.40)  LDD0624  [2]
 LDCM0308  AC46 HCT 116 C442(1.01); C466(1.01)  LDD0625  [2]
 LDCM0309  AC47 HCT 116 C442(0.87); C466(0.87)  LDD0626  [2]
 LDCM0310  AC48 HCT 116 C442(0.96); C466(0.96)  LDD0627  [2]
 LDCM0311  AC49 HCT 116 C442(1.36); C466(1.36)  LDD0628  [2]
 LDCM0313  AC50 HCT 116 C442(1.44); C466(1.44)  LDD0630  [2]
 LDCM0314  AC51 HCT 116 C442(1.30); C466(1.30)  LDD0631  [2]
 LDCM0315  AC52 HCT 116 C442(1.01); C466(1.01)  LDD0632  [2]
 LDCM0316  AC53 HCT 116 C442(1.32); C466(1.32)  LDD0633  [2]
 LDCM0317  AC54 HCT 116 C442(0.89); C466(0.89)  LDD0634  [2]
 LDCM0318  AC55 HCT 116 C442(1.20); C466(1.20)  LDD0635  [2]
 LDCM0319  AC56 HCT 116 C442(2.11); C466(2.11)  LDD0636  [2]
 LDCM0323  AC6 HCT 116 C442(1.12); C466(1.12)  LDD0640  [2]
 LDCM0332  AC68 HCT 116 C442(0.99); C466(0.99)  LDD0649  [2]
 LDCM0333  AC69 HCT 116 C442(1.13); C466(1.13)  LDD0650  [2]
 LDCM0334  AC7 HCT 116 C442(1.28); C466(1.28)  LDD0651  [2]
 LDCM0335  AC70 HCT 116 C442(0.95); C466(0.95)  LDD0652  [2]
 LDCM0336  AC71 HCT 116 C442(0.93); C466(0.93)  LDD0653  [2]
 LDCM0337  AC72 HCT 116 C442(0.94); C466(0.94)  LDD0654  [2]
 LDCM0338  AC73 HCT 116 C442(1.39); C466(1.39)  LDD0655  [2]
 LDCM0339  AC74 HCT 116 C442(1.41); C466(1.41)  LDD0656  [2]
 LDCM0340  AC75 HCT 116 C442(1.70); C466(1.70)  LDD0657  [2]
 LDCM0341  AC76 HCT 116 C442(1.03); C466(1.03)  LDD0658  [2]
 LDCM0342  AC77 HCT 116 C442(1.35); C466(1.35)  LDD0659  [2]
 LDCM0343  AC78 HCT 116 C442(0.92); C466(0.92)  LDD0660  [2]
 LDCM0344  AC79 HCT 116 C442(0.84); C466(0.84)  LDD0661  [2]
 LDCM0345  AC8 HCT 116 C442(1.15); C466(1.15)  LDD0662  [2]
 LDCM0346  AC80 HCT 116 C442(1.25); C466(1.25)  LDD0663  [2]
 LDCM0347  AC81 HCT 116 C442(0.94); C466(0.94)  LDD0664  [2]
 LDCM0348  AC82 HCT 116 C442(1.58); C466(1.58)  LDD0665  [2]
 LDCM0365  AC98 HEK-293T C442(1.15); C466(1.15)  LDD0963  [2]
 LDCM0366  AC99 HEK-293T C442(1.07); C466(1.07)  LDD0964  [2]
 LDCM0248  AKOS034007472 HCT 116 C442(0.99); C466(0.99)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C442(1.02); C466(1.02)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C442(2.79); C466(2.79)  LDD0592  [2]
 LDCM0020  ARS-1620 HCC44 C442(1.44); C466(1.44)  LDD0078  [2]
 LDCM0370  CL101 HCT 116 C442(1.44); C466(1.44)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C442(1.08); C466(1.08)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C442(1.76); C466(1.76)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C442(1.39); C466(1.39)  LDD0690  [2]
 LDCM0374  CL105 HCT 116 C442(1.53); C466(1.53)  LDD0691  [2]
 LDCM0375  CL106 HCT 116 C442(1.63); C466(1.63)  LDD0692  [2]
 LDCM0376  CL107 HCT 116 C442(1.49); C466(1.49)  LDD0693  [2]
 LDCM0377  CL108 HCT 116 C442(1.39); C466(1.39)  LDD0694  [2]
 LDCM0378  CL109 HCT 116 C442(1.60); C466(1.60)  LDD0695  [2]
 LDCM0380  CL110 HCT 116 C442(2.03); C466(2.03)  LDD0697  [2]
 LDCM0381  CL111 HCT 116 C442(1.45); C466(1.45)  LDD0698  [2]
 LDCM0382  CL112 HCT 116 C442(0.91); C466(0.91)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C442(1.10); C466(1.10)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C442(0.99); C466(0.99)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C442(1.34); C466(1.34)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C442(0.77); C466(0.77)  LDD0703  [2]
 LDCM0387  CL117 HCT 116 C442(1.96); C466(1.96)  LDD0704  [2]
 LDCM0388  CL118 HCT 116 C442(1.02); C466(1.02)  LDD0705  [2]
 LDCM0389  CL119 HCT 116 C442(1.12); C466(1.12)  LDD0706  [2]
 LDCM0391  CL120 HCT 116 C442(1.49); C466(1.49)  LDD0708  [2]
 LDCM0392  CL121 HCT 116 C442(2.23); C466(2.23)  LDD0709  [2]
 LDCM0393  CL122 HCT 116 C442(0.69); C466(0.69)  LDD0710  [2]
 LDCM0394  CL123 HCT 116 C442(1.66); C466(1.66)  LDD0711  [2]
 LDCM0395  CL124 HCT 116 C442(2.04); C466(2.04)  LDD0712  [2]
 LDCM0403  CL16 PaTu 8988t C442(1.22); C466(1.22)  LDD1282  [2]
 LDCM0404  CL17 PaTu 8988t C442(1.06); C466(1.06)  LDD1283  [2]
 LDCM0405  CL18 PaTu 8988t C442(0.96); C466(0.96)  LDD1284  [2]
 LDCM0406  CL19 PaTu 8988t C442(0.96); C466(0.96)  LDD1285  [2]
 LDCM0408  CL20 PaTu 8988t C442(1.26); C466(1.26)  LDD1287  [2]
 LDCM0409  CL21 PaTu 8988t C442(1.37); C466(1.37)  LDD1288  [2]
 LDCM0410  CL22 PaTu 8988t C442(1.05); C466(1.05)  LDD1289  [2]
 LDCM0411  CL23 PaTu 8988t C442(1.51); C466(1.51)  LDD1290  [2]
 LDCM0412  CL24 PaTu 8988t C442(0.94); C466(0.94)  LDD1291  [2]
 LDCM0413  CL25 PaTu 8988t C442(0.80); C466(0.80)  LDD1292  [2]
 LDCM0414  CL26 PaTu 8988t C442(0.94); C466(0.94)  LDD1293  [2]
 LDCM0415  CL27 PaTu 8988t C442(0.98); C466(0.98)  LDD1294  [2]
 LDCM0416  CL28 PaTu 8988t C442(1.41); C466(1.41)  LDD1295  [2]
 LDCM0417  CL29 PaTu 8988t C442(1.62); C466(1.62)  LDD1296  [2]
 LDCM0419  CL30 PaTu 8988t C442(0.74); C466(0.74)  LDD1298  [2]
 LDCM0420  CL31 PaTu 8988t C442(1.26); C466(1.26)  LDD1299  [2]
 LDCM0421  CL32 PaTu 8988t C442(1.23); C466(1.23)  LDD1300  [2]
 LDCM0422  CL33 PaTu 8988t C442(2.21); C466(2.21)  LDD1301  [2]
 LDCM0423  CL34 PaTu 8988t C442(1.54); C466(1.54)  LDD1302  [2]
 LDCM0424  CL35 PaTu 8988t C442(1.63); C466(1.63)  LDD1303  [2]
 LDCM0425  CL36 PaTu 8988t C442(1.62); C466(1.62)  LDD1304  [2]
 LDCM0426  CL37 PaTu 8988t C442(1.66); C466(1.66)  LDD1305  [2]
 LDCM0428  CL39 PaTu 8988t C442(1.29); C466(1.29)  LDD1307  [2]
 LDCM0430  CL40 PaTu 8988t C442(1.46); C466(1.46)  LDD1309  [2]
 LDCM0431  CL41 PaTu 8988t C442(1.90); C466(1.90)  LDD1310  [2]
 LDCM0432  CL42 PaTu 8988t C442(2.13); C466(2.13)  LDD1311  [2]
 LDCM0433  CL43 PaTu 8988t C442(2.43); C466(2.43)  LDD1312  [2]
 LDCM0434  CL44 PaTu 8988t C442(1.55); C466(1.55)  LDD1313  [2]
 LDCM0435  CL45 PaTu 8988t C442(1.43); C466(1.43)  LDD1314  [2]
 LDCM0436  CL46 HEK-293T C466(0.95)  LDD1034  [2]
 LDCM0437  CL47 HEK-293T C466(1.06)  LDD1035  [2]
 LDCM0438  CL48 HEK-293T C466(0.81)  LDD1036  [2]
 LDCM0439  CL49 HEK-293T C466(1.10)  LDD1037  [2]
 LDCM0441  CL50 HEK-293T C466(1.25)  LDD1039  [2]
 LDCM0442  CL51 HEK-293T C466(1.04)  LDD1040  [2]
 LDCM0443  CL52 HEK-293T C466(1.16)  LDD1041  [2]
 LDCM0444  CL53 HEK-293T C466(0.81)  LDD1042  [2]
 LDCM0445  CL54 HEK-293T C466(1.07)  LDD1043  [2]
 LDCM0446  CL55 HEK-293T C466(0.59)  LDD1044  [2]
 LDCM0447  CL56 HEK-293T C466(1.06)  LDD1045  [2]
 LDCM0448  CL57 HEK-293T C466(0.71)  LDD1046  [2]
 LDCM0449  CL58 HEK-293T C466(0.97)  LDD1047  [2]
 LDCM0450  CL59 HEK-293T C466(0.97)  LDD1048  [2]
 LDCM0452  CL60 HEK-293T C466(1.76)  LDD1050  [2]
 LDCM0453  CL61 PaTu 8988t C442(1.21); C466(1.21)  LDD1332  [2]
 LDCM0454  CL62 PaTu 8988t C442(0.79); C466(0.79)  LDD1333  [2]
 LDCM0455  CL63 PaTu 8988t C442(0.89); C466(0.89)  LDD1334  [2]
 LDCM0456  CL64 PaTu 8988t C442(0.99); C466(0.99)  LDD1335  [2]
 LDCM0457  CL65 PaTu 8988t C442(1.02); C466(1.02)  LDD1336  [2]
 LDCM0458  CL66 PaTu 8988t C442(0.99); C466(0.99)  LDD1337  [2]
 LDCM0459  CL67 PaTu 8988t C442(1.18); C466(1.18)  LDD1338  [2]
 LDCM0460  CL68 PaTu 8988t C442(1.38); C466(1.38)  LDD1339  [2]
 LDCM0461  CL69 PaTu 8988t C442(1.30); C466(1.30)  LDD1340  [2]
 LDCM0463  CL70 PaTu 8988t C442(1.20); C466(1.20)  LDD1342  [2]
 LDCM0464  CL71 PaTu 8988t C442(0.86); C466(0.86)  LDD1343  [2]
 LDCM0465  CL72 PaTu 8988t C442(1.18); C466(1.18)  LDD1344  [2]
 LDCM0466  CL73 PaTu 8988t C442(1.38); C466(1.38)  LDD1345  [2]
 LDCM0467  CL74 PaTu 8988t C442(0.89); C466(0.89)  LDD1346  [2]
 LDCM0468  Fragment33 PaTu 8988t C442(1.03); C466(1.03)  LDD1347  [2]
 LDCM0427  Fragment51 PaTu 8988t C442(1.88); C466(1.88)  LDD1306  [2]
 LDCM0021  THZ1 HCT 116 C442(1.59); C466(1.59)  LDD2173  [2]

References

1 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255