Details of the Target

General Information of Target

Target ID LDTP07042
Target Name Protein Smaug homolog 2 (SAMD4B)
Gene Name SAMD4B
Gene ID 55095
Synonyms
SMAUG2; Protein Smaug homolog 2; Smaug 2; hSmaug2; Sterile alpha motif domain-containing protein 4B; SAM domain-containing protein 4B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MMFRDQVGILAGWFKGWNECEQTVALLSLLKRVTRTQARFLQLCLEHSLADCNDIHLLES
EANSAAIVSQWQQESKEKVVSLLLSHLPLLQPGNTEAKSEYMRLLQKVLAYSIESNAFIE
ESRQLLSYALIHPATTLEDRNALALWLSHLEERLASGFRSRPEPSYHSRQGSDEWGGPAE
LGPGEAGPGWQDKPPRENGHVPFHPSSSVPPAINSIGSNANTGLPCQIHPSPLKRSMSLI
PTSPQVPGEWPSPEELGARAAFTTPDHAPLSPQSSVASSGSEQTEEQGSSRNTFQEDGSG
MKDVPSWLKSLRLHKYAALFSQMSYEEMMTLTEQHLESQNVTKGARHKIALSIQKLRERQ
SVLKSLEKDVLEGGNLRNALQELQQIIITPIKAYSVLQATVAAATTTPTAKDGAPGEPPL
PGAEPPLAHPGTDKGTEAKDPPAVENYPPPPAPAPTDGSEPAPAPVADGDIPSQFTRVMG
KVCTQLLVSRPDEENITSYLQLIEKCLTHEAFTETQKKRLLSWKQQVLKLLRTFPRKAAL
EMQNYRQQKGWAFGSNSLPIAGSVGMGVARRTQRQFPMPPRALPPGRMGLLSPSGIGGVS
PRHALTSPSLGGQGRQNLWFANPGGSNSMPSQSRSSVQRTHSLPVHSSPQAILMFPPDCP
VPGPDLEINPTLESLCLSMTEHALGDGTDKTSTI
Target Bioclass
Other
Family
SMAUG family
Subcellular location
Cytoplasm
Function Has transcriptional repressor activity. Overexpression inhibits the transcriptional activities of AP-1, p53/TP53 and CDKN1A.
Uniprot ID
Q5PRF9
Ensemble ID
ENST00000314471.10
HGNC ID
HGNC:25492

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
STPyne
 Probe Info  		K524(8.28)  LDD0277  [2]
DBIA
 Probe Info  		C20(3.77)  LDD3365  [3]
AHL-Pu-1
 Probe Info  		C20(2.38)  LDD0171  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [6]
Lodoacetamide azide
 Probe Info  		C483(0.00); C20(0.00)  LDD0037  [5]
NAIA_4
 Probe Info  		N.A.  LDD2226  [7]
IPM
 Probe Info  		C226(0.00); C20(0.00)  LDD2156  [8]
AOyne
 Probe Info  		14.00  LDD0443  [9]
NAIA_5
 Probe Info  		C506(0.00); C20(0.00); C483(0.00)  LDD2223  [7]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C266
 Probe Info  		11.47  LDD1937  [10]
C362
 Probe Info  		28.84  LDD2023  [10]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C20(2.38)  LDD0171  [4]
 LDCM0214  AC1 PaTu 8988t C506(0.24)  LDD1093  [11]
 LDCM0215  AC10 PaTu 8988t C506(0.72)  LDD1094  [11]
 LDCM0226  AC11 PaTu 8988t C506(0.44)  LDD1105  [11]
 LDCM0237  AC12 PaTu 8988t C506(0.47)  LDD1116  [11]
 LDCM0259  AC14 PaTu 8988t C506(0.49)  LDD1138  [11]
 LDCM0270  AC15 PaTu 8988t C506(0.64)  LDD1149  [11]
 LDCM0279  AC2 PaTu 8988t C506(0.38)  LDD1158  [11]
 LDCM0290  AC3 PaTu 8988t C506(0.26)  LDD1169  [11]
 LDCM0301  AC4 PaTu 8988t C506(0.31)  LDD1180  [11]
 LDCM0312  AC5 PaTu 8988t C506(0.26)  LDD1191  [11]
 LDCM0323  AC6 PaTu 8988t C506(0.72)  LDD1202  [11]
 LDCM0334  AC7 PaTu 8988t C506(0.85)  LDD1213  [11]
 LDCM0345  AC8 PaTu 8988t C506(1.02)  LDD1224  [11]
 LDCM0248  AKOS034007472 PaTu 8988t C506(0.47)  LDD1127  [11]
 LDCM0356  AKOS034007680 PaTu 8988t C506(0.90)  LDD1235  [11]
 LDCM0275  AKOS034007705 PaTu 8988t C506(0.81)  LDD1154  [11]
 LDCM0156  Aniline NCI-H1299 11.57  LDD0403  [1]
 LDCM0632  CL-Sc Hep-G2 C20(0.18)  LDD2227  [7]
 LDCM0367  CL1 PaTu 8988t C506(1.06)  LDD1246  [11]
 LDCM0368  CL10 PaTu 8988t C506(0.60)  LDD1247  [11]
 LDCM0369  CL100 PaTu 8988t C506(0.33)  LDD1248  [11]
 LDCM0370  CL101 PaTu 8988t C506(0.79)  LDD1249  [11]
 LDCM0371  CL102 PaTu 8988t C506(0.81)  LDD1250  [11]
 LDCM0372  CL103 PaTu 8988t C506(0.44)  LDD1251  [11]
 LDCM0373  CL104 PaTu 8988t C506(0.54)  LDD1252  [11]
 LDCM0375  CL106 HEK-293T C52(0.97)  LDD1579  [12]
 LDCM0377  CL108 HEK-293T C52(0.89)  LDD1581  [12]
 LDCM0379  CL11 PaTu 8988t C506(0.96)  LDD1258  [11]
 LDCM0380  CL110 HEK-293T C52(1.32)  LDD1584  [12]
 LDCM0382  CL112 HEK-293T C52(0.97)  LDD1586  [12]
 LDCM0384  CL114 HEK-293T C52(1.14)  LDD1588  [12]
 LDCM0386  CL116 HEK-293T C52(1.00)  LDD1590  [12]
 LDCM0388  CL118 HEK-293T C52(1.30)  LDD1592  [12]
 LDCM0390  CL12 PaTu 8988t C506(0.82)  LDD1269  [11]
 LDCM0391  CL120 HEK-293T C52(1.00)  LDD1595  [12]
 LDCM0393  CL122 HEK-293T C52(1.11)  LDD1597  [12]
 LDCM0395  CL124 HEK-293T C52(1.07)  LDD1599  [12]
 LDCM0397  CL126 HEK-293T C52(1.42)  LDD1601  [12]
 LDCM0399  CL128 HEK-293T C52(0.99)  LDD1603  [12]
 LDCM0400  CL13 PaTu 8988t C506(0.51)  LDD1279  [11]
 LDCM0401  CL14 PaTu 8988t C506(0.51)  LDD1280  [11]
 LDCM0402  CL15 PaTu 8988t C506(0.79)  LDD1281  [11]
 LDCM0403  CL16 HEK-293T C52(1.06)  LDD1607  [12]
 LDCM0407  CL2 PaTu 8988t C506(0.68)  LDD1286  [11]
 LDCM0414  CL26 HEK-293T C52(1.24)  LDD1618  [12]
 LDCM0416  CL28 HEK-293T C52(1.09)  LDD1620  [12]
 LDCM0418  CL3 PaTu 8988t C506(1.11)  LDD1297  [11]
 LDCM0429  CL4 PaTu 8988t C506(1.36)  LDD1308  [11]
 LDCM0430  CL40 HEK-293T C52(0.92)  LDD1634  [12]
 LDCM0436  CL46 PaTu 8988t C506(1.00)  LDD1315  [11]
 LDCM0437  CL47 PaTu 8988t C506(0.97)  LDD1316  [11]
 LDCM0438  CL48 PaTu 8988t C506(0.94)  LDD1317  [11]
 LDCM0439  CL49 PaTu 8988t C506(0.97)  LDD1318  [11]
 LDCM0440  CL5 PaTu 8988t C506(0.62)  LDD1319  [11]
 LDCM0441  CL50 PaTu 8988t C506(0.94)  LDD1320  [11]
 LDCM0442  CL51 PaTu 8988t C506(0.99)  LDD1321  [11]
 LDCM0443  CL52 PaTu 8988t C506(0.88)  LDD1322  [11]
 LDCM0444  CL53 PaTu 8988t C506(0.81)  LDD1323  [11]
 LDCM0445  CL54 PaTu 8988t C506(1.01)  LDD1324  [11]
 LDCM0446  CL55 PaTu 8988t C506(1.10)  LDD1325  [11]
 LDCM0447  CL56 PaTu 8988t C506(0.25)  LDD1326  [11]
 LDCM0448  CL57 PaTu 8988t C506(0.49)  LDD1327  [11]
 LDCM0449  CL58 PaTu 8988t C506(0.84)  LDD1328  [11]
 LDCM0450  CL59 PaTu 8988t C506(0.55)  LDD1329  [11]
 LDCM0451  CL6 PaTu 8988t C506(0.58)  LDD1330  [11]
 LDCM0452  CL60 PaTu 8988t C506(0.55)  LDD1331  [11]
 LDCM0454  CL62 HEK-293T C52(1.04)  LDD1657  [12]
 LDCM0456  CL64 HEK-293T C52(1.29)  LDD1659  [12]
 LDCM0462  CL7 PaTu 8988t C506(0.57)  LDD1341  [11]
 LDCM0467  CL74 HEK-293T C52(1.17)  LDD1670  [12]
 LDCM0469  CL76 HEK-293T C52(1.15)  LDD1672  [12]
 LDCM0473  CL8 PaTu 8988t C506(0.93)  LDD1352  [11]
 LDCM0480  CL86 HEK-293T C52(1.52)  LDD1683  [12]
 LDCM0482  CL88 HEK-293T C52(0.99)  LDD1685  [12]
 LDCM0484  CL9 PaTu 8988t C506(0.49)  LDD1363  [11]
 LDCM0486  CL91 PaTu 8988t C506(0.36)  LDD1365  [11]
 LDCM0487  CL92 PaTu 8988t C506(0.33)  LDD1366  [11]
 LDCM0488  CL93 PaTu 8988t C506(0.37)  LDD1367  [11]
 LDCM0489  CL94 PaTu 8988t C506(0.34)  LDD1368  [11]
 LDCM0490  CL95 PaTu 8988t C506(0.38)  LDD1369  [11]
 LDCM0491  CL96 PaTu 8988t C506(0.40)  LDD1370  [11]
 LDCM0492  CL97 PaTu 8988t C506(0.42)  LDD1371  [11]
 LDCM0493  CL98 PaTu 8988t C506(0.32)  LDD1372  [11]
 LDCM0494  CL99 PaTu 8988t C506(0.35)  LDD1373  [11]
 LDCM0427  Fragment51 HEK-293T C52(1.28)  LDD1631  [12]
 LDCM0022  KB02 A2780 C20(2.66)  LDD2254  [3]
 LDCM0023  KB03 A2780 C20(4.35)  LDD2671  [3]
 LDCM0024  KB05 OVCAR-3 C20(3.77)  LDD3365  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Inactive ubiquitin carboxyl-terminal hydrolase 53 (USP53) Peptidase C19 family Q70EK8
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein zeta/delta (YWHAZ) 14-3-3 family P63104
Volume-regulated anion channel subunit LRRC8E (LRRC8E) LRRC8 family Q6NSJ5
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bcl-2-like protein 10 (BCL2L10) Bcl-2 family Q9HD36
Arginine/serine-rich coiled-coil protein 2 (RSRC2) RSRC2 family Q7L4I2
Coiled-coil domain-containing protein 179 (CCDC179) . H3BU77

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
11 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402