Details of the Target

General Information of Target

Target ID LDTP05395
Target Name Aminoacylase-1 (ACY1)
Gene Name ACY1
Gene ID 95
Synonyms
Aminoacylase-1; ACY-1; EC 3.5.1.14; N-acyl-L-amino-acid amidohydrolase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTSKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVV
TVLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQ
YLEAVRRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANP
TDAFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSN
PHLKEGSVTSVNLTKLEGGVAYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEG
VTLEFAQKWMHPQVTPTDDSNPWWAAFSRVCKDMNLTLEPEIMPAATDNRYIRAVGVPAL
GFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSDS
Target Bioclass
Enzyme
Family
Peptidase M20A family
Subcellular location
Cytoplasm
Function Catalyzes the hydrolysis of N-acetylated amino acids to acetate and free amino acids.
Uniprot ID
Q03154
Ensemble ID
ENST00000404366.7
HGNC ID
HGNC:177

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.E39K .
HCT116 SNV: p.A354V .
HEC1 SNV: p.T214I DBIA    Probe Info 
HEC1B SNV: p.T214I .
Ishikawa (Heraklio) 02 ER SNV: p.N224S; p.R236M DBIA    Probe Info 
JURKAT SNV: p.R197G; p.A399T .
LNCaP clone FGC SNV: p.Q50H DBIA    Probe Info 
MOLT4 SNV: p.W309Ter .
NCIH1944 Insertion: p.V144LfsTer25 .
PF382 SNV: p.E103V .
SJSA1 SNV: p.M155I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
FBPP2
 Probe Info  		2.56  LDD0318  [1]
YN-1
 Probe Info  		100.00  LDD0444  [2]
BTD
 Probe Info  		C49(1.08)  LDD1700  [3]
HHS-475
 Probe Info  		Y188(0.68); Y105(0.94); Y107(1.18)  LDD0264  [4]
HHS-465
 Probe Info  		Y105(10.00)  LDD2237  [5]
DBIA
 Probe Info  		C116(0.89)  LDD0531  [6]
5E-2FA
 Probe Info  		N.A.  LDD2235  [7]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [8]
NAIA_5
 Probe Info  		N.A.  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C49(0.24); C116(0.70)  LDD2142  [3]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C49(0.99)  LDD2117  [3]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C49(1.09)  LDD2103  [3]
 LDCM0214  AC1 HCT 116 C116(0.89)  LDD0531  [6]
 LDCM0216  AC100 HCT 116 C116(0.73)  LDD0533  [6]
 LDCM0217  AC101 HCT 116 C116(0.79)  LDD0534  [6]
 LDCM0218  AC102 HCT 116 C116(0.80)  LDD0535  [6]
 LDCM0219  AC103 HCT 116 C116(0.89)  LDD0536  [6]
 LDCM0220  AC104 HCT 116 C116(0.83)  LDD0537  [6]
 LDCM0221  AC105 HCT 116 C116(0.86)  LDD0538  [6]
 LDCM0222  AC106 HCT 116 C116(1.02)  LDD0539  [6]
 LDCM0223  AC107 HCT 116 C116(0.96)  LDD0540  [6]
 LDCM0224  AC108 HCT 116 C116(0.56)  LDD0541  [6]
 LDCM0225  AC109 HCT 116 C116(0.72)  LDD0542  [6]
 LDCM0227  AC110 HCT 116 C116(0.78)  LDD0544  [6]
 LDCM0228  AC111 HCT 116 C116(0.65)  LDD0545  [6]
 LDCM0229  AC112 HCT 116 C116(0.75)  LDD0546  [6]
 LDCM0276  AC17 HCT 116 C116(0.93)  LDD0593  [6]
 LDCM0277  AC18 HCT 116 C116(0.82)  LDD0594  [6]
 LDCM0278  AC19 HCT 116 C116(0.96)  LDD0595  [6]
 LDCM0279  AC2 HCT 116 C116(0.97)  LDD0596  [6]
 LDCM0280  AC20 HCT 116 C116(0.96)  LDD0597  [6]
 LDCM0281  AC21 HCT 116 C116(0.76)  LDD0598  [6]
 LDCM0282  AC22 HCT 116 C116(0.87)  LDD0599  [6]
 LDCM0283  AC23 HCT 116 C116(1.29)  LDD0600  [6]
 LDCM0284  AC24 HCT 116 C116(0.70)  LDD0601  [6]
 LDCM0285  AC25 HCT 116 C116(1.09)  LDD0602  [6]
 LDCM0286  AC26 HCT 116 C116(1.35)  LDD0603  [6]
 LDCM0287  AC27 HCT 116 C116(1.01)  LDD0604  [6]
 LDCM0288  AC28 HCT 116 C116(1.34)  LDD0605  [6]
 LDCM0289  AC29 HCT 116 C116(1.33)  LDD0606  [6]
 LDCM0290  AC3 HCT 116 C116(1.09)  LDD0607  [6]
 LDCM0291  AC30 HCT 116 C116(1.24)  LDD0608  [6]
 LDCM0292  AC31 HCT 116 C116(1.39)  LDD0609  [6]
 LDCM0293  AC32 HCT 116 C116(1.60)  LDD0610  [6]
 LDCM0294  AC33 HCT 116 C116(1.29)  LDD0611  [6]
 LDCM0295  AC34 HCT 116 C116(1.76)  LDD0612  [6]
 LDCM0301  AC4 HCT 116 C116(1.04)  LDD0618  [6]
 LDCM0312  AC5 HCT 116 C116(1.04)  LDD0629  [6]
 LDCM0320  AC57 HCT 116 C116(1.30)  LDD0637  [6]
 LDCM0321  AC58 HCT 116 C116(1.53)  LDD0638  [6]
 LDCM0322  AC59 HCT 116 C116(1.61)  LDD0639  [6]
 LDCM0324  AC60 HCT 116 C116(1.47)  LDD0641  [6]
 LDCM0325  AC61 HCT 116 C116(1.40)  LDD0642  [6]
 LDCM0326  AC62 HCT 116 C116(1.52)  LDD0643  [6]
 LDCM0327  AC63 HCT 116 C116(1.37)  LDD0644  [6]
 LDCM0328  AC64 HCT 116 C116(1.70)  LDD0645  [6]
 LDCM0329  AC65 HCT 116 C116(1.76)  LDD0646  [6]
 LDCM0330  AC66 HCT 116 C116(1.72)  LDD0647  [6]
 LDCM0331  AC67 HCT 116 C116(1.60)  LDD0648  [6]
 LDCM0365  AC98 HCT 116 C116(0.73)  LDD0682  [6]
 LDCM0366  AC99 HCT 116 C116(0.62)  LDD0683  [6]
 LDCM0369  CL100 HCT 116 C116(1.03)  LDD0686  [6]
 LDCM0374  CL105 HCT 116 C116(1.21)  LDD0691  [6]
 LDCM0375  CL106 HCT 116 C116(1.20)  LDD0692  [6]
 LDCM0376  CL107 HCT 116 C116(1.09)  LDD0693  [6]
 LDCM0377  CL108 HCT 116 C116(1.26)  LDD0694  [6]
 LDCM0378  CL109 HCT 116 C116(1.04)  LDD0695  [6]
 LDCM0380  CL110 HCT 116 C116(1.41)  LDD0697  [6]
 LDCM0381  CL111 HCT 116 C116(0.92)  LDD0698  [6]
 LDCM0382  CL112 HCT 116 C116(1.22)  LDD0699  [6]
 LDCM0383  CL113 HCT 116 C116(1.12)  LDD0700  [6]
 LDCM0384  CL114 HCT 116 C116(1.29)  LDD0701  [6]
 LDCM0385  CL115 HCT 116 C116(1.56)  LDD0702  [6]
 LDCM0386  CL116 HCT 116 C116(1.08)  LDD0703  [6]
 LDCM0396  CL125 HCT 116 C116(1.11)  LDD0713  [6]
 LDCM0397  CL126 HCT 116 C116(1.21)  LDD0714  [6]
 LDCM0398  CL127 HCT 116 C116(1.40)  LDD0715  [6]
 LDCM0399  CL128 HCT 116 C116(1.28)  LDD0716  [6]
 LDCM0403  CL16 HCT 116 C116(1.47)  LDD0720  [6]
 LDCM0404  CL17 HCT 116 C116(1.04)  LDD0721  [6]
 LDCM0405  CL18 HCT 116 C116(1.45)  LDD0722  [6]
 LDCM0406  CL19 HCT 116 C116(1.48)  LDD0723  [6]
 LDCM0408  CL20 HCT 116 C116(1.59)  LDD0725  [6]
 LDCM0409  CL21 HCT 116 C116(1.23)  LDD0726  [6]
 LDCM0410  CL22 HCT 116 C116(0.96)  LDD0727  [6]
 LDCM0411  CL23 HCT 116 C116(1.75)  LDD0728  [6]
 LDCM0412  CL24 HCT 116 C116(1.55)  LDD0729  [6]
 LDCM0413  CL25 HCT 116 C116(1.45)  LDD0730  [6]
 LDCM0414  CL26 HCT 116 C116(1.28)  LDD0731  [6]
 LDCM0415  CL27 HCT 116 C116(1.57)  LDD0732  [6]
 LDCM0416  CL28 HCT 116 C116(1.89)  LDD0733  [6]
 LDCM0417  CL29 HCT 116 C116(2.17)  LDD0734  [6]
 LDCM0419  CL30 HCT 116 C116(2.13)  LDD0736  [6]
 LDCM0486  CL91 HCT 116 C116(0.84)  LDD0803  [6]
 LDCM0487  CL92 HCT 116 C116(0.94)  LDD0804  [6]
 LDCM0488  CL93 HCT 116 C116(0.96)  LDD0805  [6]
 LDCM0489  CL94 HCT 116 C116(0.91)  LDD0806  [6]
 LDCM0490  CL95 HCT 116 C116(1.12)  LDD0807  [6]
 LDCM0491  CL96 HCT 116 C116(1.02)  LDD0808  [6]
 LDCM0492  CL97 HCT 116 C116(1.06)  LDD0809  [6]
 LDCM0493  CL98 HCT 116 C116(0.96)  LDD0810  [6]
 LDCM0494  CL99 HCT 116 C116(0.90)  LDD0811  [6]
 LDCM0116  HHS-0101 DM93 Y188(0.68); Y105(0.94); Y107(1.18)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y105(0.94); Y188(1.07); Y107(1.16)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y105(0.92); Y188(1.42); Y107(1.44)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y188(0.89); Y105(0.93); Y107(1.42)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y188(0.68); Y105(0.80); Y107(1.01)  LDD0268  [4]
 LDCM0022  KB02 A101D C49(1.46)  LDD2250  [10]
 LDCM0023  KB03 A2058 C81(2.07); C49(2.01)  LDD2670  [10]
 LDCM0024  KB05 MOLM-13 C49(2.28)  LDD3333  [10]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C49(1.03)  LDD2092  [3]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C49(1.29)  LDD2094  [3]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C49(0.23); C116(0.32)  LDD2096  [3]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C49(0.79)  LDD2097  [3]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C49(1.02)  LDD2098  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C49(0.76)  LDD2099  [3]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C49(0.23); C116(1.26)  LDD2100  [3]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C49(1.58)  LDD2105  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C49(1.07)  LDD2107  [3]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C49(0.29)  LDD2108  [3]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C49(0.44)  LDD2109  [3]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C116(0.78)  LDD2110  [3]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C49(0.33); C116(0.44)  LDD2114  [3]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C116(0.46)  LDD2116  [3]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C49(1.93); C116(0.51)  LDD2118  [3]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C49(1.10)  LDD2120  [3]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C49(2.90); C116(0.44)  LDD2122  [3]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C49(0.46); C116(0.35)  LDD2124  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C49(0.82)  LDD2125  [3]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C49(4.43); C116(0.34)  LDD2126  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C49(0.74)  LDD2127  [3]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C49(0.80)  LDD2128  [3]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C49(0.53)  LDD2134  [3]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C49(1.08)  LDD1700  [3]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C49(1.31)  LDD2143  [3]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C49(0.19)  LDD2145  [3]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C49(0.78)  LDD2146  [3]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C49(0.48)  LDD2148  [3]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C49(3.83); C116(0.35)  LDD2149  [3]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C116(0.43)  LDD2151  [3]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C49(1.87)  LDD2153  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Protein N-terminal glutamine amidohydrolase (NTAQ1) NTAQ1 family Q96HA8
Aminoacylase-1 (ACY1) Peptidase M20A family Q03154
Terminal nucleotidyltransferase 5B (TENT5B) TENT family Q96A09
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
T-cell leukemia homeobox protein 3 (TLX3) . O43711
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pre-mRNA-splicing factor SPF27 (BCAS2) SPF27 family O75934
Proline-rich protein 35 (PRR35) . P0CG20
Telomeric repeat-binding factor 1 (TERF1) . P54274

The Drug(s) Related To This Target

Approved
Click To Hide/Show 3 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Acetylcysteine Small molecular drug DB06151
Aspartic Acid Small molecular drug DB00128
Copper . DB09130

References

1 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
2 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840