Details of the Target

General Information of Target

Target ID LDTP03902
Target Name DNA mismatch repair protein Mlh1 (MLH1)
Gene Name MLH1
Gene ID 4292
Synonyms
COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSFVAGVIRRLDETVVNRIAAGEVIQRPANAIKEMIENCLDAKSTSIQVIVKEGGLKLIQ
IQDNGTGIRKEDLDIVCERFTTSKLQSFEDLASISTYGFRGEALASISHVAHVTITTKTA
DGKCAYRASYSDGKLKAPPKPCAGNQGTQITVEDLFYNIATRRKALKNPSEEYGKILEVV
GRYSVHNAGISFSVKKQGETVADVRTLPNASTVDNIRSIFGNAVSRELIEIGCEDKTLAF
KMNGYISNANYSVKKCIFLLFINHRLVESTSLRKAIETVYAAYLPKNTHPFLYLSLEISP
QNVDVNVHPTKHEVHFLHEESILERVQQHIESKLLGSNSSRMYFTQTLLPGLAGPSGEMV
KSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDIS
SGRARQQDEEMLELPAPAEVAAKNQSLEGDTTKGTSEMSEKRGPTSSNPRKRHREDSDVE
MVEDDSRKEMTAACTPRRRIINLTSVLSLQEEINEQGHEVLREMLHNHSFVGCVNPQWAL
AQHQTKLYLLNTTKLSEELFYQILIYDFANFGVLRLSEPAPLFDLAMLALDSPESGWTEE
DGPKEGLAEYIVEFLKKKAEMLADYFSLEIDEEGNLIGLPLLIDNYVPPLEGLPIFILRL
ATEVNWDEEKECFESLSKECAMFYSIRKQYISEESTLSGQQSEVPGSIPNSWKWTVEHIV
YKALRSHILPPKHFTEDGNILQLANLPDLYKVFERC
Target Type
Literature-reported
Target Bioclass
Other
Family
DNA mismatch repair MutL/HexB family
Subcellular location
Nucleus
Function
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
TTD ID
T35799
Uniprot ID
P40692
DrugMap ID
TTISG27
Ensemble ID
ENST00000231790.8
HGNC ID
HGNC:7127

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.T117M .
DU145 SNV: p.A586V DBIA    Probe Info 
HCT116 SNV: p.S252Ter .
HCT15 SNV: p.A120S DBIA    Probe Info 
HSC4 SNV: p.E632K DBIA    Probe Info 
ICC137 Substitution: p.K618A DBIA    Probe Info 
IGR1 SNV: p.R265C DBIA    Probe Info 
IGROV1 Deletion: p.S505VfsTer3 DBIA    Probe Info 
LS180 SNV: p.T117M .
MDAMB157 SNV: p.R205T DBIA    Probe Info 
MIAPACA2 SNV: p.T270I .
PC9 Deletion: p.L104WfsTer4 .
RKO SNV: p.L323M DBIA    Probe Info 
SAOS2 SNV: p.V201A .
SNU1 SNV: p.R226Ter DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		13.16  LDD0402  [1]
BTD
 Probe Info  		C494(0.99)  LDD2109  [2]
HHS-475
 Probe Info  		Y379(0.77)  LDD0264  [3]
DBIA
 Probe Info  		C256(1.00)  LDD0078  [4]
4-Iodoacetamidophenylacetylene
 Probe Info  		C142(0.00); C233(0.00); C494(0.00); C77(0.00)  LDD0038  [5]
IA-alkyne
 Probe Info  		C142(0.00); C680(0.00); C494(0.00); C233(0.00)  LDD0036  [5]
Lodoacetamide azide
 Probe Info  		C142(0.00); C233(0.00); C39(0.00); C494(0.00)  LDD0037  [5]
IPM
 Probe Info  		N.A.  LDD0025  [6]
TFBX
 Probe Info  		C233(0.00); C256(0.00)  LDD0027  [6]
Acrolein
 Probe Info  		C77(0.00); C494(0.00)  LDD0217  [7]
Crotonaldehyde
 Probe Info  		C77(0.00); C494(0.00)  LDD0219  [7]
Methacrolein
 Probe Info  		N.A.  LDD0218  [7]
W1
 Probe Info  		N.A.  LDD0236  [8]
NAIA_5
 Probe Info  		C39(0.00); C256(0.00); C77(0.00)  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 PaTu 8988t C142(1.31); C256(1.17)  LDD1093  [4]
 LDCM0215  AC10 PaTu 8988t C77(1.26); C256(1.51)  LDD1094  [4]
 LDCM0216  AC100 PaTu 8988t C77(0.77); C256(1.12)  LDD1095  [4]
 LDCM0217  AC101 PaTu 8988t C77(1.11); C256(1.25)  LDD1096  [4]
 LDCM0218  AC102 PaTu 8988t C77(0.91); C256(1.21)  LDD1097  [4]
 LDCM0219  AC103 PaTu 8988t C77(0.81); C256(0.99)  LDD1098  [4]
 LDCM0220  AC104 PaTu 8988t C77(0.83); C256(1.05)  LDD1099  [4]
 LDCM0221  AC105 PaTu 8988t C77(1.12); C256(1.07)  LDD1100  [4]
 LDCM0222  AC106 PaTu 8988t C77(0.94); C256(1.01)  LDD1101  [4]
 LDCM0223  AC107 PaTu 8988t C77(1.07); C256(1.06)  LDD1102  [4]
 LDCM0224  AC108 PaTu 8988t C77(0.99); C256(1.03)  LDD1103  [4]
 LDCM0225  AC109 PaTu 8988t C77(1.24); C256(1.37)  LDD1104  [4]
 LDCM0226  AC11 PaTu 8988t C77(1.18); C256(1.59)  LDD1105  [4]
 LDCM0227  AC110 PaTu 8988t C77(0.75); C256(1.11)  LDD1106  [4]
 LDCM0228  AC111 PaTu 8988t C77(1.10); C256(1.28)  LDD1107  [4]
 LDCM0229  AC112 PaTu 8988t C77(0.93); C256(1.06)  LDD1108  [4]
 LDCM0230  AC113 PaTu 8988t C256(0.95)  LDD1109  [4]
 LDCM0231  AC114 PaTu 8988t C256(1.07)  LDD1110  [4]
 LDCM0232  AC115 PaTu 8988t C256(1.11)  LDD1111  [4]
 LDCM0233  AC116 PaTu 8988t C256(1.22)  LDD1112  [4]
 LDCM0234  AC117 PaTu 8988t C256(0.99)  LDD1113  [4]
 LDCM0235  AC118 PaTu 8988t C256(0.98)  LDD1114  [4]
 LDCM0236  AC119 PaTu 8988t C256(1.07)  LDD1115  [4]
 LDCM0237  AC12 PaTu 8988t C77(1.24); C256(1.24)  LDD1116  [4]
 LDCM0238  AC120 PaTu 8988t C256(1.14)  LDD1117  [4]
 LDCM0239  AC121 PaTu 8988t C256(1.36)  LDD1118  [4]
 LDCM0240  AC122 PaTu 8988t C256(1.07)  LDD1119  [4]
 LDCM0241  AC123 PaTu 8988t C256(0.93)  LDD1120  [4]
 LDCM0242  AC124 PaTu 8988t C256(1.34)  LDD1121  [4]
 LDCM0243  AC125 PaTu 8988t C256(0.94)  LDD1122  [4]
 LDCM0244  AC126 PaTu 8988t C256(1.06)  LDD1123  [4]
 LDCM0245  AC127 PaTu 8988t C256(1.14)  LDD1124  [4]
 LDCM0246  AC128 PaTu 8988t C256(1.18)  LDD1125  [4]
 LDCM0247  AC129 PaTu 8988t C256(0.99)  LDD1126  [4]
 LDCM0249  AC130 PaTu 8988t C256(1.03)  LDD1128  [4]
 LDCM0250  AC131 PaTu 8988t C256(1.12)  LDD1129  [4]
 LDCM0251  AC132 PaTu 8988t C256(1.34)  LDD1130  [4]
 LDCM0252  AC133 PaTu 8988t C256(1.55)  LDD1131  [4]
 LDCM0253  AC134 PaTu 8988t C256(1.25)  LDD1132  [4]
 LDCM0254  AC135 PaTu 8988t C256(1.60)  LDD1133  [4]
 LDCM0255  AC136 PaTu 8988t C256(1.30)  LDD1134  [4]
 LDCM0256  AC137 PaTu 8988t C256(1.39)  LDD1135  [4]
 LDCM0257  AC138 PaTu 8988t C256(1.00)  LDD1136  [4]
 LDCM0258  AC139 PaTu 8988t C256(1.27)  LDD1137  [4]
 LDCM0259  AC14 PaTu 8988t C77(0.89); C256(1.48)  LDD1138  [4]
 LDCM0260  AC140 PaTu 8988t C256(1.12)  LDD1139  [4]
 LDCM0261  AC141 PaTu 8988t C256(1.05)  LDD1140  [4]
 LDCM0262  AC142 PaTu 8988t C256(1.28)  LDD1141  [4]
 LDCM0263  AC143 PaTu 8988t C142(1.04); C256(1.03)  LDD1142  [4]
 LDCM0264  AC144 PaTu 8988t C142(1.22); C256(1.01)  LDD1143  [4]
 LDCM0265  AC145 PaTu 8988t C142(0.83); C256(0.88)  LDD1144  [4]
 LDCM0266  AC146 PaTu 8988t C142(0.87); C256(1.06)  LDD1145  [4]
 LDCM0267  AC147 PaTu 8988t C142(0.99); C256(0.96)  LDD1146  [4]
 LDCM0268  AC148 PaTu 8988t C142(1.01); C256(0.99)  LDD1147  [4]
 LDCM0269  AC149 PaTu 8988t C142(0.98); C256(0.96)  LDD1148  [4]
 LDCM0270  AC15 PaTu 8988t C77(1.01); C256(1.49)  LDD1149  [4]
 LDCM0271  AC150 PaTu 8988t C142(1.86); C256(1.14)  LDD1150  [4]
 LDCM0272  AC151 PaTu 8988t C142(0.95); C256(1.42)  LDD1151  [4]
 LDCM0273  AC152 PaTu 8988t C142(1.19); C256(1.17)  LDD1152  [4]
 LDCM0274  AC153 PaTu 8988t C142(0.99); C256(1.16)  LDD1153  [4]
 LDCM0621  AC154 PaTu 8988t C142(1.07); C256(1.23)  LDD2166  [4]
 LDCM0622  AC155 PaTu 8988t C142(1.38); C256(1.23)  LDD2167  [4]
 LDCM0623  AC156 PaTu 8988t C142(1.24); C256(1.19)  LDD2168  [4]
 LDCM0624  AC157 PaTu 8988t C142(1.28); C256(0.95)  LDD2169  [4]
 LDCM0276  AC17 PaTu 8988t C256(0.77)  LDD1155  [4]
 LDCM0277  AC18 PaTu 8988t C256(1.11)  LDD1156  [4]
 LDCM0278  AC19 PaTu 8988t C256(0.97)  LDD1157  [4]
 LDCM0279  AC2 PaTu 8988t C142(1.32); C256(1.09)  LDD1158  [4]
 LDCM0280  AC20 PaTu 8988t C256(1.09)  LDD1159  [4]
 LDCM0281  AC21 PaTu 8988t C256(1.04)  LDD1160  [4]
 LDCM0282  AC22 PaTu 8988t C256(1.23)  LDD1161  [4]
 LDCM0283  AC23 PaTu 8988t C256(1.07)  LDD1162  [4]
 LDCM0284  AC24 PaTu 8988t C256(1.14)  LDD1163  [4]
 LDCM0285  AC25 PaTu 8988t C77(0.94)  LDD1164  [4]
 LDCM0286  AC26 PaTu 8988t C77(1.08)  LDD1165  [4]
 LDCM0287  AC27 PaTu 8988t C77(0.92)  LDD1166  [4]
 LDCM0288  AC28 PaTu 8988t C77(1.07)  LDD1167  [4]
 LDCM0289  AC29 PaTu 8988t C77(1.00)  LDD1168  [4]
 LDCM0290  AC3 PaTu 8988t C142(0.93); C256(1.05)  LDD1169  [4]
 LDCM0291  AC30 PaTu 8988t C77(0.89)  LDD1170  [4]
 LDCM0292  AC31 PaTu 8988t C77(0.90)  LDD1171  [4]
 LDCM0293  AC32 PaTu 8988t C77(1.01)  LDD1172  [4]
 LDCM0294  AC33 PaTu 8988t C77(0.94)  LDD1173  [4]
 LDCM0295  AC34 PaTu 8988t C77(1.16)  LDD1174  [4]
 LDCM0296  AC35 PaTu 8988t C142(1.11)  LDD1175  [4]
 LDCM0297  AC36 PaTu 8988t C142(1.42)  LDD1176  [4]
 LDCM0298  AC37 PaTu 8988t C142(1.22)  LDD1177  [4]
 LDCM0299  AC38 PaTu 8988t C142(1.13)  LDD1178  [4]
 LDCM0300  AC39 PaTu 8988t C142(1.34)  LDD1179  [4]
 LDCM0301  AC4 PaTu 8988t C142(0.87); C256(1.08)  LDD1180  [4]
 LDCM0302  AC40 PaTu 8988t C142(1.56)  LDD1181  [4]
 LDCM0303  AC41 PaTu 8988t C142(0.92)  LDD1182  [4]
 LDCM0304  AC42 PaTu 8988t C142(1.10)  LDD1183  [4]
 LDCM0305  AC43 PaTu 8988t C142(1.29)  LDD1184  [4]
 LDCM0306  AC44 PaTu 8988t C142(1.19)  LDD1185  [4]
 LDCM0307  AC45 PaTu 8988t C142(1.44)  LDD1186  [4]
 LDCM0312  AC5 PaTu 8988t C142(0.60); C256(1.20)  LDD1191  [4]
 LDCM0323  AC6 PaTu 8988t C77(1.16); C256(0.99)  LDD1202  [4]
 LDCM0332  AC68 PaTu 8988t C256(1.14)  LDD1211  [4]
 LDCM0333  AC69 PaTu 8988t C256(1.06)  LDD1212  [4]
 LDCM0334  AC7 PaTu 8988t C77(0.91); C256(1.27)  LDD1213  [4]
 LDCM0335  AC70 PaTu 8988t C256(1.15)  LDD1214  [4]
 LDCM0336  AC71 PaTu 8988t C256(1.17)  LDD1215  [4]
 LDCM0337  AC72 PaTu 8988t C256(0.90)  LDD1216  [4]
 LDCM0338  AC73 PaTu 8988t C256(0.96)  LDD1217  [4]
 LDCM0339  AC74 PaTu 8988t C256(1.16)  LDD1218  [4]
 LDCM0340  AC75 PaTu 8988t C256(1.06)  LDD1219  [4]
 LDCM0341  AC76 PaTu 8988t C256(1.10)  LDD1220  [4]
 LDCM0342  AC77 PaTu 8988t C256(1.41)  LDD1221  [4]
 LDCM0343  AC78 PaTu 8988t C256(0.93)  LDD1222  [4]
 LDCM0344  AC79 PaTu 8988t C256(1.27)  LDD1223  [4]
 LDCM0345  AC8 PaTu 8988t C77(0.89); C256(1.38)  LDD1224  [4]
 LDCM0346  AC80 PaTu 8988t C256(1.10)  LDD1225  [4]
 LDCM0347  AC81 PaTu 8988t C256(1.13)  LDD1226  [4]
 LDCM0348  AC82 PaTu 8988t C256(1.23)  LDD1227  [4]
 LDCM0349  AC83 PaTu 8988t C256(1.23)  LDD1228  [4]
 LDCM0350  AC84 PaTu 8988t C256(1.15)  LDD1229  [4]
 LDCM0351  AC85 PaTu 8988t C256(0.99)  LDD1230  [4]
 LDCM0352  AC86 PaTu 8988t C256(0.92)  LDD1231  [4]
 LDCM0353  AC87 PaTu 8988t C256(1.01)  LDD1232  [4]
 LDCM0354  AC88 PaTu 8988t C256(1.32)  LDD1233  [4]
 LDCM0355  AC89 PaTu 8988t C256(0.88)  LDD1234  [4]
 LDCM0357  AC90 PaTu 8988t C256(1.14)  LDD1236  [4]
 LDCM0358  AC91 PaTu 8988t C256(1.23)  LDD1237  [4]
 LDCM0359  AC92 PaTu 8988t C256(1.26)  LDD1238  [4]
 LDCM0360  AC93 PaTu 8988t C256(1.00)  LDD1239  [4]
 LDCM0361  AC94 PaTu 8988t C256(0.91)  LDD1240  [4]
 LDCM0362  AC95 PaTu 8988t C256(1.12)  LDD1241  [4]
 LDCM0363  AC96 PaTu 8988t C256(1.22)  LDD1242  [4]
 LDCM0364  AC97 PaTu 8988t C256(1.10)  LDD1243  [4]
 LDCM0365  AC98 PaTu 8988t C77(1.00); C256(1.15)  LDD1244  [4]
 LDCM0366  AC99 PaTu 8988t C77(1.18); C256(1.07)  LDD1245  [4]
 LDCM0248  AKOS034007472 PaTu 8988t C77(1.09); C256(1.96)  LDD1127  [4]
 LDCM0356  AKOS034007680 PaTu 8988t C77(1.51); C256(1.64)  LDD1235  [4]
 LDCM0275  AKOS034007705 PaTu 8988t C77(1.25); C256(1.55)  LDD1154  [4]
 LDCM0156  Aniline NCI-H1299 12.37  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C256(1.00)  LDD0078  [4]
 LDCM0102  BDHI 8 Jurkat C680(4.24)  LDD0204  [10]
 LDCM0108  Chloroacetamide HeLa C77(0.00); C494(0.00)  LDD0222  [7]
 LDCM0632  CL-Sc Hep-G2 C39(1.72); C256(1.70); C233(1.21); C77(0.96)  LDD2227  [9]
 LDCM0367  CL1 PaTu 8988t C256(0.91)  LDD1246  [4]
 LDCM0368  CL10 PaTu 8988t C256(0.94)  LDD1247  [4]
 LDCM0369  CL100 PaTu 8988t C142(0.74); C256(0.96)  LDD1248  [4]
 LDCM0370  CL101 PaTu 8988t C77(0.94); C256(0.96)  LDD1249  [4]
 LDCM0371  CL102 PaTu 8988t C77(1.07); C256(1.24)  LDD1250  [4]
 LDCM0372  CL103 PaTu 8988t C77(1.14); C256(2.15)  LDD1251  [4]
 LDCM0373  CL104 PaTu 8988t C77(1.04); C256(1.33)  LDD1252  [4]
 LDCM0374  CL105 PaTu 8988t C256(0.79)  LDD1253  [4]
 LDCM0375  CL106 PaTu 8988t C256(0.80)  LDD1254  [4]
 LDCM0376  CL107 PaTu 8988t C256(0.77)  LDD1255  [4]
 LDCM0377  CL108 PaTu 8988t C256(0.74)  LDD1256  [4]
 LDCM0378  CL109 PaTu 8988t C256(0.87)  LDD1257  [4]
 LDCM0379  CL11 PaTu 8988t C256(0.76)  LDD1258  [4]
 LDCM0380  CL110 PaTu 8988t C256(1.06)  LDD1259  [4]
 LDCM0381  CL111 PaTu 8988t C256(1.45)  LDD1260  [4]
 LDCM0382  CL112 PaTu 8988t C77(0.92)  LDD1261  [4]
 LDCM0383  CL113 PaTu 8988t C77(0.98)  LDD1262  [4]
 LDCM0384  CL114 PaTu 8988t C77(0.84)  LDD1263  [4]
 LDCM0385  CL115 PaTu 8988t C77(0.92)  LDD1264  [4]
 LDCM0386  CL116 PaTu 8988t C77(1.40)  LDD1265  [4]
 LDCM0387  CL117 PaTu 8988t C142(1.58)  LDD1266  [4]
 LDCM0388  CL118 PaTu 8988t C142(0.93)  LDD1267  [4]
 LDCM0389  CL119 PaTu 8988t C142(0.87)  LDD1268  [4]
 LDCM0390  CL12 PaTu 8988t C256(0.83)  LDD1269  [4]
 LDCM0391  CL120 PaTu 8988t C142(1.32)  LDD1270  [4]
 LDCM0400  CL13 PaTu 8988t C256(1.49)  LDD1279  [4]
 LDCM0401  CL14 PaTu 8988t C256(1.15)  LDD1280  [4]
 LDCM0402  CL15 PaTu 8988t C256(1.11)  LDD1281  [4]
 LDCM0403  CL16 PaTu 8988t C77(0.89)  LDD1282  [4]
 LDCM0404  CL17 PaTu 8988t C77(0.81)  LDD1283  [4]
 LDCM0405  CL18 PaTu 8988t C77(0.86)  LDD1284  [4]
 LDCM0406  CL19 PaTu 8988t C77(0.85)  LDD1285  [4]
 LDCM0407  CL2 PaTu 8988t C256(1.17)  LDD1286  [4]
 LDCM0408  CL20 PaTu 8988t C77(0.94)  LDD1287  [4]
 LDCM0409  CL21 PaTu 8988t C77(0.79)  LDD1288  [4]
 LDCM0410  CL22 PaTu 8988t C77(0.80)  LDD1289  [4]
 LDCM0411  CL23 PaTu 8988t C77(0.76)  LDD1290  [4]
 LDCM0412  CL24 PaTu 8988t C77(1.02)  LDD1291  [4]
 LDCM0413  CL25 PaTu 8988t C77(0.83)  LDD1292  [4]
 LDCM0414  CL26 PaTu 8988t C77(0.92)  LDD1293  [4]
 LDCM0415  CL27 PaTu 8988t C77(0.86)  LDD1294  [4]
 LDCM0416  CL28 PaTu 8988t C77(0.75)  LDD1295  [4]
 LDCM0417  CL29 PaTu 8988t C77(0.95)  LDD1296  [4]
 LDCM0418  CL3 PaTu 8988t C256(1.13)  LDD1297  [4]
 LDCM0419  CL30 PaTu 8988t C77(0.87)  LDD1298  [4]
 LDCM0420  CL31 PaTu 8988t C142(1.26); C256(0.94)  LDD1299  [4]
 LDCM0421  CL32 PaTu 8988t C142(0.95); C256(1.03)  LDD1300  [4]
 LDCM0422  CL33 PaTu 8988t C142(0.82); C256(0.96)  LDD1301  [4]
 LDCM0423  CL34 PaTu 8988t C142(1.12); C256(0.89)  LDD1302  [4]
 LDCM0424  CL35 PaTu 8988t C256(1.02); C142(1.18)  LDD1303  [4]
 LDCM0425  CL36 PaTu 8988t C142(0.83); C256(0.67)  LDD1304  [4]
 LDCM0426  CL37 PaTu 8988t C142(0.86); C256(1.05)  LDD1305  [4]
 LDCM0428  CL39 PaTu 8988t C142(1.11); C256(1.13)  LDD1307  [4]
 LDCM0429  CL4 PaTu 8988t C256(1.10)  LDD1308  [4]
 LDCM0430  CL40 PaTu 8988t C142(1.06); C256(0.96)  LDD1309  [4]
 LDCM0431  CL41 PaTu 8988t C142(0.81); C256(0.92)  LDD1310  [4]
 LDCM0432  CL42 PaTu 8988t C142(1.14); C256(1.40)  LDD1311  [4]
 LDCM0433  CL43 PaTu 8988t C142(1.05); C256(1.38)  LDD1312  [4]
 LDCM0434  CL44 PaTu 8988t C142(1.31); C256(1.21)  LDD1313  [4]
 LDCM0435  CL45 PaTu 8988t C142(1.07); C256(1.03)  LDD1314  [4]
 LDCM0436  CL46 PaTu 8988t C256(0.87)  LDD1315  [4]
 LDCM0437  CL47 PaTu 8988t C256(0.97)  LDD1316  [4]
 LDCM0438  CL48 PaTu 8988t C256(1.11)  LDD1317  [4]
 LDCM0439  CL49 PaTu 8988t C256(0.95)  LDD1318  [4]
 LDCM0440  CL5 PaTu 8988t C256(1.48)  LDD1319  [4]
 LDCM0441  CL50 PaTu 8988t C256(0.99)  LDD1320  [4]
 LDCM0442  CL51 PaTu 8988t C256(1.21)  LDD1321  [4]
 LDCM0443  CL52 PaTu 8988t C256(1.03)  LDD1322  [4]
 LDCM0444  CL53 PaTu 8988t C256(1.11)  LDD1323  [4]
 LDCM0445  CL54 PaTu 8988t C256(0.82)  LDD1324  [4]
 LDCM0446  CL55 PaTu 8988t C256(0.82)  LDD1325  [4]
 LDCM0447  CL56 PaTu 8988t C256(0.42)  LDD1326  [4]
 LDCM0448  CL57 PaTu 8988t C256(1.53)  LDD1327  [4]
 LDCM0449  CL58 PaTu 8988t C256(1.21)  LDD1328  [4]
 LDCM0450  CL59 PaTu 8988t C256(1.74)  LDD1329  [4]
 LDCM0451  CL6 PaTu 8988t C256(0.99)  LDD1330  [4]
 LDCM0452  CL60 PaTu 8988t C256(1.44)  LDD1331  [4]
 LDCM0453  CL61 PaTu 8988t C77(0.92); C256(1.02)  LDD1332  [4]
 LDCM0454  CL62 PaTu 8988t C77(1.11); C256(0.75)  LDD1333  [4]
 LDCM0455  CL63 PaTu 8988t C77(0.86); C256(0.87)  LDD1334  [4]
 LDCM0456  CL64 PaTu 8988t C77(1.11); C256(0.96)  LDD1335  [4]
 LDCM0457  CL65 PaTu 8988t C77(0.80); C256(1.01)  LDD1336  [4]
 LDCM0458  CL66 PaTu 8988t C77(1.04); C256(1.02)  LDD1337  [4]
 LDCM0459  CL67 PaTu 8988t C77(0.81); C256(1.36)  LDD1338  [4]
 LDCM0460  CL68 PaTu 8988t C77(0.95); C256(1.67)  LDD1339  [4]
 LDCM0461  CL69 PaTu 8988t C77(1.15); C256(1.36)  LDD1340  [4]
 LDCM0462  CL7 PaTu 8988t C256(1.29)  LDD1341  [4]
 LDCM0463  CL70 PaTu 8988t C77(1.42); C256(1.33)  LDD1342  [4]
 LDCM0464  CL71 PaTu 8988t C77(0.99); C256(1.01)  LDD1343  [4]
 LDCM0465  CL72 PaTu 8988t C77(1.12); C256(0.81)  LDD1344  [4]
 LDCM0466  CL73 PaTu 8988t C77(1.28); C256(1.21)  LDD1345  [4]
 LDCM0467  CL74 PaTu 8988t C77(0.89); C256(1.03)  LDD1346  [4]
 LDCM0469  CL76 PaTu 8988t C142(1.23); C256(1.14)  LDD1348  [4]
 LDCM0470  CL77 PaTu 8988t C142(1.58); C256(0.98)  LDD1349  [4]
 LDCM0471  CL78 PaTu 8988t C142(1.54); C256(1.19)  LDD1350  [4]
 LDCM0472  CL79 PaTu 8988t C142(1.21); C256(1.04)  LDD1351  [4]
 LDCM0473  CL8 PaTu 8988t C256(1.04)  LDD1352  [4]
 LDCM0474  CL80 PaTu 8988t C142(1.41); C256(1.18)  LDD1353  [4]
 LDCM0475  CL81 PaTu 8988t C142(1.00); C256(1.20)  LDD1354  [4]
 LDCM0476  CL82 PaTu 8988t C142(0.92); C256(1.03)  LDD1355  [4]
 LDCM0477  CL83 PaTu 8988t C142(1.03); C256(1.21)  LDD1356  [4]
 LDCM0478  CL84 PaTu 8988t C142(1.48); C256(1.12)  LDD1357  [4]
 LDCM0479  CL85 PaTu 8988t C142(1.42); C256(1.00)  LDD1358  [4]
 LDCM0480  CL86 PaTu 8988t C142(1.32); C256(1.04)  LDD1359  [4]
 LDCM0481  CL87 PaTu 8988t C142(1.39); C256(1.41)  LDD1360  [4]
 LDCM0482  CL88 PaTu 8988t C142(1.36); C256(1.35)  LDD1361  [4]
 LDCM0483  CL89 PaTu 8988t C142(1.30); C256(1.30)  LDD1362  [4]
 LDCM0484  CL9 PaTu 8988t C256(1.01)  LDD1363  [4]
 LDCM0485  CL90 PaTu 8988t C142(1.06); C256(1.09)  LDD1364  [4]
 LDCM0486  CL91 PaTu 8988t C142(1.36); C256(0.93)  LDD1365  [4]
 LDCM0487  CL92 PaTu 8988t C142(1.06); C256(0.94)  LDD1366  [4]
 LDCM0488  CL93 PaTu 8988t C142(1.43); C256(0.93)  LDD1367  [4]
 LDCM0489  CL94 PaTu 8988t C142(1.29); C256(1.06)  LDD1368  [4]
 LDCM0490  CL95 PaTu 8988t C142(0.70); C256(1.07)  LDD1369  [4]
 LDCM0491  CL96 PaTu 8988t C142(0.67); C256(0.87)  LDD1370  [4]
 LDCM0492  CL97 PaTu 8988t C142(0.64); C256(0.96)  LDD1371  [4]
 LDCM0493  CL98 PaTu 8988t C142(1.07); C256(1.30)  LDD1372  [4]
 LDCM0494  CL99 PaTu 8988t C142(0.63); C256(1.05)  LDD1373  [4]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C142(3.35); C494(0.98)  LDD1702  [2]
 LDCM0625  F8 Ramos C39(1.90); C77(1.22); C233(0.47); C142(0.65)  LDD2187  [11]
 LDCM0573  Fragment11 Ramos C39(13.58); C256(9.15); C77(0.20); C233(1.90)  LDD2190  [11]
 LDCM0575  Fragment13 Ramos C142(3.78)  LDD2192  [11]
 LDCM0576  Fragment14 Ramos C39(1.17); C256(1.64); C77(1.07); C233(1.14)  LDD2193  [11]
 LDCM0582  Fragment23 Ramos C142(3.19)  LDD2196  [11]
 LDCM0586  Fragment28 Ramos C39(0.71); C77(0.44); C233(0.83); C142(0.52)  LDD2198  [11]
 LDCM0588  Fragment30 Ramos C142(3.31)  LDD2199  [11]
 LDCM0468  Fragment33 PaTu 8988t C77(1.14); C256(1.16)  LDD1347  [4]
 LDCM0566  Fragment4 Ramos C39(0.93); C256(2.11); C77(0.84)  LDD2184  [11]
 LDCM0427  Fragment51 PaTu 8988t C142(1.11); C256(1.18)  LDD1306  [4]
 LDCM0610  Fragment52 Ramos C142(1.88)  LDD2204  [11]
 LDCM0614  Fragment56 Ramos C142(1.31)  LDD2205  [11]
 LDCM0569  Fragment7 Ramos C39(1.29); C256(0.61); C77(1.32); C233(0.85)  LDD2186  [11]
 LDCM0116  HHS-0101 DM93 Y379(0.77)  LDD0264  [3]
 LDCM0117  HHS-0201 DM93 Y379(0.35)  LDD0265  [3]
 LDCM0118  HHS-0301 DM93 Y379(0.76)  LDD0266  [3]
 LDCM0119  HHS-0401 DM93 Y379(0.75)  LDD0267  [3]
 LDCM0120  HHS-0701 DM93 Y379(0.50)  LDD0268  [3]
 LDCM0022  KB02 HCT 116 C494(1.30)  LDD0080  [4]
 LDCM0023  KB03 HCT 116 C494(1.29)  LDD0081  [4]
 LDCM0024  KB05 HCT 116 C494(1.49)  LDD0082  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C494(0.99)  LDD2109  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C494(1.08)  LDD2127  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C77(0.46)  LDD2137  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 13 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Actin, cytoplasmic 2 (ACTG1) Actin family P63261
Fanconi anemia group J protein (BRIP1) DEAD box helicase family Q9BX63
Mismatch repair endonuclease PMS2 (PMS2) DNA mismatch repair MutL/HexB family P54278
Heat shock cognate 71 kDa protein (HSPA8) Heat shock protein 70 family P11142
DNA helicase MCM9 (MCM9) MCM family Q9NXL9
Nucleoside diphosphate kinase, mitochondrial (NME4) NDK family O00746
Cathepsin B (CTSB) Peptidase C1 family P07858
Proteasome subunit alpha type-1 (PSMA1) Peptidase T1A family P25786
Cyclin-dependent kinase-like 5 (CDKL5) CMGC Ser/Thr protein kinase family O76039
Tyrosine-protein phosphatase non-receptor type 3 (PTPN3) Protein-tyrosine phosphatase family P26045
7-methylguanosine phosphate-specific 5'-nucleotidase (NT5C3B) Pyrimidine 5'-nucleotidase family Q969T7
BTB/POZ domain-containing protein 2 (BTBD2) . Q9BX70
RING finger protein 37 (UBOX5) . O94941
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Annexin A2 (ANXA2) Annexin family P07355
Importin subunit alpha-1 (KPNA2) Importin alpha family P52292
Importin subunit alpha-6 (KPNA5) Importin alpha family O15131
Transcription factor
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Transcription factor AP-2-delta (TFAP2D) AP-2 family Q7Z6R9
Zinc finger protein 212 (ZNF212) Krueppel C2H2-type zinc-finger protein family Q9UDV6
Zinc finger protein 774 (ZNF774) Krueppel C2H2-type zinc-finger protein family Q6NX45
Zinc finger protein 90 homolog (ZFP90) Krueppel C2H2-type zinc-finger protein family Q8TF47
Myogenin (MYOG) . P15173
Zinc finger protein 474 (ZNF474) . Q6S9Z5
Other
Click To Hide/Show 35 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Apoptosis-stimulating of p53 protein 1 (PPP1R13B) ASPP family Q96KQ4
Protein chibby homolog 2 (CBY2) Chibby family Q8NA61
DNA mismatch repair protein Mlh3 (MLH3) DNA mismatch repair MutL/HexB family Q9UHC1
PMS1 protein homolog 1 (PMS1) DNA mismatch repair MutL/HexB family P54277
DNA mismatch repair protein Msh3 (MSH3) DNA mismatch repair MutS family P20585
Protein FAM228A (FAM228A) FAM228 family Q86W67
Protein FAM50B (FAM50B) FAM50 family Q9Y247
Protein FAM90A1 (FAM90A1) FAM90 family Q86YD7
Fascin (FSCN1) Fascin family Q16658
Radial spoke head protein 3 homolog (RSPH3) Flagellar radial spoke RSP3 family Q86UC2
Desmin (DES) Intermediate filament family P17661
Pre-mRNA-splicing factor ISY1 homolog (ISY1) ISY1 family Q9ULR0
Methyl-CpG-binding domain protein 3-like 1 (MBD3L1) MBD3L family Q8WWY6
Negative elongation factor A (NELFA) NELF-A family Q9H3P2
Proline-rich protein 5-like (PRR5L) PROTOR family Q6MZQ0
RNA-binding protein 48 (RBM48) RBM48 family Q5RL73
Spectrin alpha chain, non-erythrocytic 1 (SPTAN1) Spectrin family Q13813
Splicing factor U2AF 35 kDa subunit (U2AF1) Splicing factor SR family Q01081
Pre-mRNA-splicing factor SYF2 (SYF2) SYF2 family O95926
Protein TASOR 2 (TASOR2) TASOR family Q5VWN6
Thymosin beta-4 (TMSB4X) Thymosin beta family P62328
Cell division cycle-associated 7-like protein (CDCA7L) . Q96GN5
Coiled-coil domain-containing protein 33 (CCDC33) . Q8N5R6
Enkurin domain-containing protein 1 (ENKD1) . Q9H0I2
FERM domain-containing protein 6 (FRMD6) . Q96NE9
Keratinocyte proline-rich protein (KPRP) . Q5T749
Melanoma-associated antigen 8 (MAGEA8) . P43361
MORN repeat-containing protein 3 (MORN3) . Q6PF18
Period circadian protein homolog 2 (PER2) . O15055
RPA-related protein RADX (RADX) . Q6NSI4
Spermatogenesis-associated protein 8 (SPATA8) . Q6RVD6
Testis-specific gene 13 protein (TSGA13) . Q96PP4
Uncharacterized protein C9orf152 (C9orf152) . Q5JTZ5
Zinc finger CCCH domain-containing protein 11A (ZC3H11A) . O75152
Zinc finger matrin-type protein 5 (ZMAT5) . Q9UDW3

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
8 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
11 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578