Details of the Target

General Information of Target

Target ID LDTP03877
Target Name Macrophage-capping protein (CAPG)
Gene Name CAPG
Gene ID 822
Synonyms
AFCP; MCP; Macrophage-capping protein; Actin regulatory protein CAP-G
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MYTAIPQSGSPFPGSVQDPGLHVWRVEKLKPVPVAQENQGVFFSGDSYLVLHNGPEEVSH
LHLWIGQQSSRDEQGACAVLAVHLNTLLGERPVQHREVQGNESDLFMSYFPRGLKYQEGG
VESAFHKTSTGAPAAIKKLYQVKGKKNIRATERALNWDSFNTGDCFILDLGQNIFAWCGG
KSNILERNKARDLALAIRDSERQGKAQVEIVTDGEEPAEMIQVLGPKPALKEGNPEEDLT
ADKANAQAAALYKVSDATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRK
ANEKERQAALQVAEGFISRMQYAPNTQVEILPQGHESPIFKQFFKDWK
Target Bioclass
Other
Family
Villin/gelsolin family
Subcellular location
Nucleus
Function
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Uniprot ID
P40121
Ensemble ID
ENST00000263867.9
HGNC ID
HGNC:1474

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCT116 SNV: p.Q68Ter .
LNCaP clone FGC SNV: p.G288W DBIA    Probe Info 
MCC26 Substitution: p.Q207Ter .
OVKATE SNV: p.T3A .
SNU423 SNV: p.N161S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 16 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
TH211
 Probe Info  		Y116(17.41); Y109(15.69)  LDD0260  [1]
ONAyne
 Probe Info  		K115(0.94); K127(1.24)  LDD0274  [2]
STPyne
 Probe Info  		K115(7.79); K127(6.53); K137(5.56); K143(2.70)  LDD0277  [2]
DBIA
 Probe Info  		C77(1.01)  LDD3310  [3]
HHS-482
 Probe Info  		Y109(0.82); Y116(0.96)  LDD0285  [4]
HHS-475
 Probe Info  		Y116(0.81)  LDD0264  [5]
Acrolein
 Probe Info  		H126(0.00); H335(0.00)  LDD0221  [6]
5E-2FA
 Probe Info  		H83(0.00); H335(0.00); H126(0.00)  LDD2235  [7]
m-APA
 Probe Info  		H83(0.00); H335(0.00); H126(0.00)  LDD2231  [7]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [8]
IA-alkyne
 Probe Info  		C165(0.00); C178(0.00); C77(0.00); C282(0.00)  LDD0036  [8]
IPIAA_H
 Probe Info  		N.A.  LDD0030  [9]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [9]
Lodoacetamide azide
 Probe Info  		C77(0.00); C282(0.00); C290(0.00)  LDD0037  [8]
ATP probe
 Probe Info  		K115(0.00); K127(0.00)  LDD0035  [10]
NAIA_5
 Probe Info  		N.A.  LDD2223  [11]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
VE-P
 Probe Info  		N.A.  LDD0396  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0630  CCW28-3 231MFP C290(1.34)  LDD2214  [13]
 LDCM0108  Chloroacetamide HeLa H126(0.00); C77(0.00)  LDD0222  [6]
 LDCM0372  CL103 HEK-293T C290(0.99)  LDD1576  [14]
 LDCM0376  CL107 HEK-293T C290(0.96)  LDD1580  [14]
 LDCM0381  CL111 HEK-293T C290(1.02)  LDD1585  [14]
 LDCM0385  CL115 HEK-293T C290(1.11)  LDD1589  [14]
 LDCM0389  CL119 HEK-293T C290(1.13)  LDD1593  [14]
 LDCM0394  CL123 HEK-293T C290(0.84)  LDD1598  [14]
 LDCM0398  CL127 HEK-293T C290(1.19)  LDD1602  [14]
 LDCM0402  CL15 HEK-293T C290(0.94)  LDD1606  [14]
 LDCM0415  CL27 HEK-293T C290(1.08)  LDD1619  [14]
 LDCM0418  CL3 HEK-293T C290(1.14)  LDD1622  [14]
 LDCM0428  CL39 HEK-293T C290(0.97)  LDD1632  [14]
 LDCM0455  CL63 HEK-293T C290(1.20)  LDD1658  [14]
 LDCM0481  CL87 HEK-293T C290(0.96)  LDD1684  [14]
 LDCM0494  CL99 HEK-293T C290(1.05)  LDD1697  [14]
 LDCM0495  E2913 HEK-293T C290(1.08)  LDD1698  [14]
 LDCM0468  Fragment33 HEK-293T C290(1.03)  LDD1671  [14]
 LDCM0116  HHS-0101 DM93 Y116(0.81)  LDD0264  [5]
 LDCM0117  HHS-0201 DM93 Y116(0.39)  LDD0265  [5]
 LDCM0118  HHS-0301 DM93 Y116(0.77)  LDD0266  [5]
 LDCM0119  HHS-0401 DM93 Y116(0.44)  LDD0267  [5]
 LDCM0120  HHS-0701 DM93 Y116(0.51)  LDD0268  [5]
 LDCM0107  IAA HeLa H126(0.00); H335(0.00)  LDD0221  [6]
 LDCM0123  JWB131 DM93 Y109(0.82); Y116(0.96)  LDD0285  [4]
 LDCM0124  JWB142 DM93 Y109(1.14); Y116(0.48)  LDD0286  [4]
 LDCM0125  JWB146 DM93 Y109(10.39); Y116(0.74)  LDD0287  [4]
 LDCM0126  JWB150 DM93 Y109(20.00); Y116(3.58)  LDD0288  [4]
 LDCM0127  JWB152 DM93 Y109(20.00); Y116(2.18)  LDD0289  [4]
 LDCM0128  JWB198 DM93 Y109(0.25); Y116(0.78)  LDD0290  [4]
 LDCM0129  JWB202 DM93 Y109(7.19); Y116(2.82)  LDD0291  [4]
 LDCM0130  JWB211 DM93 Y109(0.39); Y116(1.13)  LDD0292  [4]
 LDCM0022  KB02 A-172 C77(1.29)  LDD2251  [3]
 LDCM0023  KB03 42-MG-BA C77(1.60)  LDD2661  [3]
 LDCM0024  KB05 COLO792 C77(1.01)  LDD3310  [3]
 LDCM0109  NEM HeLa H126(0.00); H335(0.00)  LDD0223  [6]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase SIAH1 (SIAH1) SINA (Seven in absentia) family Q8IUQ4
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Sprouty-related, EVH1 domain-containing protein 1 (SPRED1) . Q7Z699

References

1 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
5 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
10 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
11 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
12 Pharmacological Targeting of Vacuolar H(+)-ATPase via Subunit V1G Combats Multidrug-Resistant Cancer. Cell Chem Biol. 2020 Nov 19;27(11):1359-1370.e8. doi: 10.1016/j.chembiol.2020.06.011. Epub 2020 Jul 9.
13 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.
14 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402