Details of the Target

General Information of Target

Target ID LDTP18479
Target Name Asparagine synthetase domain-containing protein 1 (ASNSD1)
Gene Name ASNSD1
Gene ID 54529
Synonyms
NS3TP1; Asparagine synthetase domain-containing protein 1; HCV NS3-transactivated protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MERSGPSEVTGSDASGPDPQLAVTMGFTGFGKKARTFDLEAMFEQTRRTAVERSRKTLEA
REKEEEMNREKELRRQNEDIEPTSSRSNVVRDCSKSSSRDTSSSESEQSSDSSDDELIGP
PLPPKMVGKPVNFMEEDILGPLPPPLNEEEEEAEEEEEEEEEEENPVHKIPDSHEITLKH
GTKTVSALGLDPSGARLVTGGYDYDVKFWDFAGMDASFKAFRSLQPCECHQIKSLQYSNT
GDMILVVSGSSQAKVIDRDGFEVMECIKGDQYIVDMANTKGHTAMLHTGSWHPKIKGEFM
TCSNDATVRTWEVENPKKQKSVFKPRTMQGKKVIPTTCTYSRDGNLIAAACQNGSIQIWD
RNLTVHPKFHYKQAHDSGTDTSCVTFSYDGNVLASRGGDDSLKLWDIRQFNKPLFSASGL
PTMFPMTDCCFSPDDKLIVTGTSIQRGCGSGKLVFFERRTFQRVYEIDITDASVVRCLWH
PKLNQIMVGTGNGLAKVYYDPNKSQRGAKLCVVKTQRKAKQAETLTQDYIITPHALPMFR
EPRQRSTRKQLEKDRLDPLKSHKPEPPVAGPGRGGRVGTHGGTLSSYIVKNIALDKTDDS
NPREAILRHAKAAEDSPYWVSPAYSKTQPKTMFAQVESDDEEAKNEPEWKKRKI
Target Bioclass
Other
Uniprot ID
Q9NWL6
Ensemble ID
ENST00000260952.9
HGNC ID
HGNC:24910

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A375 SNV: p.P551S .
CCK81 SNV: p.D42G .
COLO792 SNV: p.V322A .
HCT116 SNV: p.Q169H .
HT115 SNV: p.I616L .
LOXIMVI SNV: p.G470R .
MCC26 SNV: p.L299F .
NCIH2286 SNV: p.L237V; p.A476S .
TOV21G SNV: p.E13D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Johansson_61
 Probe Info  		_(7.48)  LDD1485  [1]
DBIA
 Probe Info  		C378(2.13)  LDD0080  [2]
IPM
 Probe Info  		N.A.  LDD0005  [3]
AOyne
 Probe Info  		14.20  LDD0443  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 PaTu 8988t C155(0.33)  LDD1093  [2]
 LDCM0215  AC10 PaTu 8988t C155(1.76)  LDD1094  [2]
 LDCM0226  AC11 PaTu 8988t C155(1.34)  LDD1105  [2]
 LDCM0230  AC113 PaTu 8988t C155(0.86)  LDD1109  [2]
 LDCM0231  AC114 PaTu 8988t C155(1.02)  LDD1110  [2]
 LDCM0232  AC115 PaTu 8988t C155(0.86)  LDD1111  [2]
 LDCM0233  AC116 PaTu 8988t C155(1.21)  LDD1112  [2]
 LDCM0234  AC117 PaTu 8988t C155(0.84)  LDD1113  [2]
 LDCM0235  AC118 PaTu 8988t C155(1.02)  LDD1114  [2]
 LDCM0236  AC119 PaTu 8988t C155(1.17)  LDD1115  [2]
 LDCM0237  AC12 PaTu 8988t C155(1.19)  LDD1116  [2]
 LDCM0238  AC120 PaTu 8988t C155(1.43)  LDD1117  [2]
 LDCM0239  AC121 PaTu 8988t C155(1.10)  LDD1118  [2]
 LDCM0240  AC122 PaTu 8988t C155(1.70)  LDD1119  [2]
 LDCM0241  AC123 PaTu 8988t C155(0.85)  LDD1120  [2]
 LDCM0242  AC124 PaTu 8988t C155(1.40)  LDD1121  [2]
 LDCM0243  AC125 PaTu 8988t C155(0.86)  LDD1122  [2]
 LDCM0244  AC126 PaTu 8988t C155(1.31)  LDD1123  [2]
 LDCM0245  AC127 PaTu 8988t C155(1.13)  LDD1124  [2]
 LDCM0246  AC128 PaTu 8988t C155(0.81)  LDD1125  [2]
 LDCM0247  AC129 PaTu 8988t C155(1.01)  LDD1126  [2]
 LDCM0249  AC130 PaTu 8988t C155(0.74)  LDD1128  [2]
 LDCM0250  AC131 PaTu 8988t C155(0.60)  LDD1129  [2]
 LDCM0251  AC132 PaTu 8988t C155(0.77)  LDD1130  [2]
 LDCM0252  AC133 PaTu 8988t C155(0.81)  LDD1131  [2]
 LDCM0253  AC134 PaTu 8988t C155(0.83)  LDD1132  [2]
 LDCM0254  AC135 PaTu 8988t C155(1.00)  LDD1133  [2]
 LDCM0255  AC136 PaTu 8988t C155(0.82)  LDD1134  [2]
 LDCM0256  AC137 PaTu 8988t C155(0.64)  LDD1135  [2]
 LDCM0257  AC138 PaTu 8988t C155(0.57)  LDD1136  [2]
 LDCM0258  AC139 PaTu 8988t C155(0.71)  LDD1137  [2]
 LDCM0259  AC14 PaTu 8988t C155(1.60)  LDD1138  [2]
 LDCM0260  AC140 PaTu 8988t C155(0.75)  LDD1139  [2]
 LDCM0261  AC141 PaTu 8988t C155(1.05)  LDD1140  [2]
 LDCM0262  AC142 PaTu 8988t C155(0.87)  LDD1141  [2]
 LDCM0270  AC15 PaTu 8988t C155(1.41)  LDD1149  [2]
 LDCM0279  AC2 PaTu 8988t C155(0.27)  LDD1158  [2]
 LDCM0280  AC20 HEK-293T C155(0.96)  LDD1519  [5]
 LDCM0281  AC21 HEK-293T C378(1.43)  LDD1520  [5]
 LDCM0283  AC23 HEK-293T C378(1.03)  LDD1522  [5]
 LDCM0285  AC25 PaTu 8988t C155(1.36)  LDD1164  [2]
 LDCM0286  AC26 PaTu 8988t C155(1.63)  LDD1165  [2]
 LDCM0287  AC27 PaTu 8988t C155(1.27)  LDD1166  [2]
 LDCM0288  AC28 PaTu 8988t C155(1.33)  LDD1167  [2]
 LDCM0289  AC29 PaTu 8988t C155(0.72)  LDD1168  [2]
 LDCM0290  AC3 PaTu 8988t C155(0.28)  LDD1169  [2]
 LDCM0291  AC30 PaTu 8988t C155(1.15)  LDD1170  [2]
 LDCM0292  AC31 PaTu 8988t C155(0.58)  LDD1171  [2]
 LDCM0293  AC32 PaTu 8988t C155(0.70)  LDD1172  [2]
 LDCM0294  AC33 PaTu 8988t C155(1.79)  LDD1173  [2]
 LDCM0295  AC34 PaTu 8988t C155(1.06)  LDD1174  [2]
 LDCM0297  AC36 HEK-293T C155(0.87)  LDD1536  [5]
 LDCM0298  AC37 HEK-293T C378(1.49)  LDD1537  [5]
 LDCM0300  AC39 HEK-293T C378(0.96)  LDD1539  [5]
 LDCM0301  AC4 PaTu 8988t C155(0.31)  LDD1180  [2]
 LDCM0306  AC44 HEK-293T C155(0.82)  LDD1545  [5]
 LDCM0307  AC45 HEK-293T C378(1.61)  LDD1546  [5]
 LDCM0309  AC47 HEK-293T C378(1.01)  LDD1548  [5]
 LDCM0312  AC5 PaTu 8988t C155(0.34)  LDD1191  [2]
 LDCM0315  AC52 HEK-293T C155(0.75)  LDD1554  [5]
 LDCM0316  AC53 HEK-293T C378(1.39)  LDD1555  [5]
 LDCM0318  AC55 HEK-293T C378(1.06)  LDD1557  [5]
 LDCM0320  AC57 PaTu 8988t C155(0.68)  LDD1199  [2]
 LDCM0321  AC58 PaTu 8988t C155(1.02)  LDD1200  [2]
 LDCM0322  AC59 PaTu 8988t C155(0.70)  LDD1201  [2]
 LDCM0323  AC6 PaTu 8988t C155(1.17)  LDD1202  [2]
 LDCM0324  AC60 PaTu 8988t C155(0.95)  LDD1203  [2]
 LDCM0325  AC61 PaTu 8988t C155(0.80)  LDD1204  [2]
 LDCM0326  AC62 PaTu 8988t C155(1.12)  LDD1205  [2]
 LDCM0327  AC63 PaTu 8988t C155(0.84)  LDD1206  [2]
 LDCM0328  AC64 PaTu 8988t C155(0.97)  LDD1207  [2]
 LDCM0329  AC65 PaTu 8988t C155(0.70)  LDD1208  [2]
 LDCM0330  AC66 PaTu 8988t C155(0.73)  LDD1209  [2]
 LDCM0331  AC67 PaTu 8988t C155(0.85)  LDD1210  [2]
 LDCM0332  AC68 PaTu 8988t C155(0.90)  LDD1211  [2]
 LDCM0333  AC69 PaTu 8988t C155(1.22)  LDD1212  [2]
 LDCM0334  AC7 PaTu 8988t C155(1.41)  LDD1213  [2]
 LDCM0335  AC70 PaTu 8988t C155(0.90)  LDD1214  [2]
 LDCM0336  AC71 PaTu 8988t C155(1.11)  LDD1215  [2]
 LDCM0337  AC72 PaTu 8988t C155(0.90)  LDD1216  [2]
 LDCM0338  AC73 PaTu 8988t C155(0.97)  LDD1217  [2]
 LDCM0339  AC74 PaTu 8988t C155(0.96)  LDD1218  [2]
 LDCM0340  AC75 PaTu 8988t C155(1.21)  LDD1219  [2]
 LDCM0341  AC76 PaTu 8988t C155(0.84)  LDD1220  [2]
 LDCM0342  AC77 PaTu 8988t C155(1.07)  LDD1221  [2]
 LDCM0343  AC78 PaTu 8988t C155(1.21)  LDD1222  [2]
 LDCM0344  AC79 PaTu 8988t C155(1.01)  LDD1223  [2]
 LDCM0345  AC8 PaTu 8988t C155(1.18)  LDD1224  [2]
 LDCM0346  AC80 PaTu 8988t C155(1.10)  LDD1225  [2]
 LDCM0347  AC81 PaTu 8988t C155(0.94)  LDD1226  [2]
 LDCM0348  AC82 PaTu 8988t C155(0.91)  LDD1227  [2]
 LDCM0248  AKOS034007472 PaTu 8988t C155(1.56)  LDD1127  [2]
 LDCM0356  AKOS034007680 PaTu 8988t C155(1.17)  LDD1235  [2]
 LDCM0275  AKOS034007705 PaTu 8988t C155(1.49)  LDD1154  [2]
 LDCM0369  CL100 PaTu 8988t C155(0.23)  LDD1248  [2]
 LDCM0370  CL101 PaTu 8988t C155(1.24)  LDD1249  [2]
 LDCM0371  CL102 PaTu 8988t C155(1.11)  LDD1250  [2]
 LDCM0372  CL103 PaTu 8988t C155(1.99)  LDD1251  [2]
 LDCM0373  CL104 PaTu 8988t C155(1.13)  LDD1252  [2]
 LDCM0375  CL106 HEK-293T C378(0.70)  LDD1579  [5]
 LDCM0379  CL11 HEK-293T C378(1.23)  LDD1583  [5]
 LDCM0380  CL110 HEK-293T C378(0.79)  LDD1584  [5]
 LDCM0382  CL112 PaTu 8988t C155(0.76)  LDD1261  [2]
 LDCM0383  CL113 PaTu 8988t C155(1.11)  LDD1262  [2]
 LDCM0384  CL114 PaTu 8988t C155(0.95)  LDD1263  [2]
 LDCM0385  CL115 PaTu 8988t C155(1.00)  LDD1264  [2]
 LDCM0386  CL116 PaTu 8988t C155(1.03)  LDD1265  [2]
 LDCM0388  CL118 HEK-293T C378(1.04)  LDD1592  [5]
 LDCM0393  CL122 HEK-293T C378(0.85)  LDD1597  [5]
 LDCM0396  CL125 PaTu 8988t C155(0.77)  LDD1275  [2]
 LDCM0397  CL126 PaTu 8988t C155(0.96)  LDD1276  [2]
 LDCM0398  CL127 PaTu 8988t C155(0.72)  LDD1277  [2]
 LDCM0399  CL128 PaTu 8988t C155(0.93)  LDD1278  [2]
 LDCM0401  CL14 HEK-293T C378(1.08)  LDD1605  [5]
 LDCM0403  CL16 PaTu 8988t C155(0.98)  LDD1282  [2]
 LDCM0404  CL17 PaTu 8988t C155(0.92)  LDD1283  [2]
 LDCM0405  CL18 PaTu 8988t C155(1.20)  LDD1284  [2]
 LDCM0406  CL19 PaTu 8988t C155(0.96)  LDD1285  [2]
 LDCM0407  CL2 HEK-293T C378(1.13)  LDD1611  [5]
 LDCM0408  CL20 PaTu 8988t C155(0.99)  LDD1287  [2]
 LDCM0409  CL21 PaTu 8988t C155(1.01)  LDD1288  [2]
 LDCM0410  CL22 PaTu 8988t C155(1.09)  LDD1289  [2]
 LDCM0411  CL23 PaTu 8988t C155(1.07)  LDD1290  [2]
 LDCM0412  CL24 PaTu 8988t C155(1.10)  LDD1291  [2]
 LDCM0413  CL25 PaTu 8988t C155(1.02)  LDD1292  [2]
 LDCM0414  CL26 PaTu 8988t C155(0.98)  LDD1293  [2]
 LDCM0415  CL27 PaTu 8988t C155(1.05)  LDD1294  [2]
 LDCM0416  CL28 PaTu 8988t C155(0.81)  LDD1295  [2]
 LDCM0417  CL29 PaTu 8988t C155(1.06)  LDD1296  [2]
 LDCM0419  CL30 PaTu 8988t C155(1.34)  LDD1298  [2]
 LDCM0421  CL32 HEK-293T C155(0.92)  LDD1625  [5]
 LDCM0422  CL33 HEK-293T C378(1.37)  LDD1626  [5]
 LDCM0424  CL35 HEK-293T C378(1.11)  LDD1628  [5]
 LDCM0434  CL44 HEK-293T C155(0.76)  LDD1638  [5]
 LDCM0435  CL45 HEK-293T C378(1.01)  LDD1639  [5]
 LDCM0436  CL46 PaTu 8988t C155(1.05)  LDD1315  [2]
 LDCM0437  CL47 PaTu 8988t C155(0.96)  LDD1316  [2]
 LDCM0438  CL48 PaTu 8988t C155(1.18)  LDD1317  [2]
 LDCM0439  CL49 PaTu 8988t C155(1.39)  LDD1318  [2]
 LDCM0441  CL50 PaTu 8988t C155(1.27)  LDD1320  [2]
 LDCM0442  CL51 PaTu 8988t C155(1.55)  LDD1321  [2]
 LDCM0443  CL52 PaTu 8988t C155(1.23)  LDD1322  [2]
 LDCM0444  CL53 PaTu 8988t C155(1.46)  LDD1323  [2]
 LDCM0445  CL54 PaTu 8988t C155(1.23)  LDD1324  [2]
 LDCM0446  CL55 PaTu 8988t C155(1.18)  LDD1325  [2]
 LDCM0447  CL56 PaTu 8988t C155(0.40)  LDD1326  [2]
 LDCM0448  CL57 PaTu 8988t C155(1.78)  LDD1327  [2]
 LDCM0449  CL58 PaTu 8988t C155(1.49)  LDD1328  [2]
 LDCM0450  CL59 PaTu 8988t C155(2.05)  LDD1329  [2]
 LDCM0452  CL60 PaTu 8988t C155(1.56)  LDD1331  [2]
 LDCM0454  CL62 HEK-293T C378(1.17)  LDD1657  [5]
 LDCM0460  CL68 HEK-293T C155(0.87)  LDD1663  [5]
 LDCM0461  CL69 HEK-293T C378(1.13)  LDD1664  [5]
 LDCM0464  CL71 HEK-293T C378(1.37)  LDD1667  [5]
 LDCM0467  CL74 HEK-293T C378(1.14)  LDD1670  [5]
 LDCM0473  CL8 HEK-293T C155(1.06)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C155(1.09)  LDD1677  [5]
 LDCM0475  CL81 HEK-293T C378(1.11)  LDD1678  [5]
 LDCM0477  CL83 HEK-293T C378(0.99)  LDD1680  [5]
 LDCM0480  CL86 HEK-293T C378(0.79)  LDD1683  [5]
 LDCM0484  CL9 HEK-293T C378(1.14)  LDD1687  [5]
 LDCM0486  CL91 PaTu 8988t C155(0.25)  LDD1365  [2]
 LDCM0487  CL92 PaTu 8988t C155(0.23)  LDD1366  [2]
 LDCM0488  CL93 PaTu 8988t C155(0.25)  LDD1367  [2]
 LDCM0489  CL94 PaTu 8988t C155(0.27)  LDD1368  [2]
 LDCM0490  CL95 PaTu 8988t C155(0.27)  LDD1369  [2]
 LDCM0491  CL96 PaTu 8988t C155(0.33)  LDD1370  [2]
 LDCM0492  CL97 PaTu 8988t C155(0.30)  LDD1371  [2]
 LDCM0493  CL98 PaTu 8988t C155(0.28)  LDD1372  [2]
 LDCM0494  CL99 PaTu 8988t C155(0.26)  LDD1373  [2]
 LDCM0427  Fragment51 HEK-293T C378(1.32)  LDD1631  [5]
 LDCM0569  Fragment7 Jurkat _(7.48)  LDD1485  [1]
 LDCM0022  KB02 HCT 116 C378(2.13)  LDD0080  [2]
 LDCM0023  KB03 HCT 116 C378(1.45)  LDD0081  [2]
 LDCM0024  KB05 HCT 116 C378(2.40)  LDD0082  [2]

References

1 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402