Details of the Target

General Information of Target

Target ID LDTP15626
Target Name Glutathione-specific gamma-glutamylcyclotransferase 2 (CHAC2)
Gene Name CHAC2
Gene ID 494143
Synonyms
Glutathione-specific gamma-glutamylcyclotransferase 2; Gamma-GCG 2; EC 4.3.2.7; Cation transport regulator-like protein 2
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRQTLPLLLLTVLRPSWADPPQEKVPLFRVTQQGPWGSSGSNATDSPCEGLPAADATALT
LANRNLERLPGCLPRTLRSLDASHNLLRALSTSELGHLEQLQVLTLRHNRIAALRWGPGG
PAGLHTLDLSYNQLAALPPCTGPALSSLRALALAGNPLRALQPRAFACFPALQLLNLSCT
ALGRGAQGGIAEAAFAGEDGAPLVTLEVLDLSGTFLERVESGWIRDLPKLTSLYLRKMPR
LTTLEGDIFKMTPNLQQLDCQDSPALASVATHIFQDTPHLQVLLFQNCNLSSFPPWTLDS
SQVLSINLFGNPLTCSCDLSWLLTDAKRTVLSRAADTMCAPAAGSSGPFSASLSLSQLPG
VCQSDQSTTLGASHPPCFNRSTYAQGTTVAPSAAPATRPAGDQQSVSKAPNVGSRTIAAW
PHSDAREGTAPSTTNSVAGHSNSSVFPRAASTTRTQHRGEHAPELVLEPDISAASTPLAS
KLLGPFPTSWDRSISSPQPGQRTHATPQAPNPSLSEGEIPVLLLDDYSEEEEGRKEEVGT
PHQDVPCDYHPCKHLQTPCAELQRRWRCRCPGLSGEDTIPDPPRLQGVTETTDTSALVHW
CAPNSVVHGYQIRYSAEGWAGNQSVVGVIYATARQHPLYGLSPGTTYRVCVLAANRAGLS
QPRSSGWRSPCAAFTTKPSFALLLSGLCAASGLLLASTVVLSACLCRRGQTLGLQRCDTH
LVAYKNPAFDDYPLGLQTVS
Target Bioclass
Enzyme
Family
Gamma-glutamylcyclotransferase family, ChaC subfamily
Subcellular location
Cytoplasm, cytosol
Function Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides.
Uniprot ID
Q8WUX2
Ensemble ID
ENST00000295304.5
HGNC ID
HGNC:32363

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
NAIA_5
 Probe Info  		C59(1.50)  LDD2227  [1]
AHL-Pu-1
 Probe Info  		C59(3.36)  LDD0169  [2]
DBIA
 Probe Info  		C59(1.71)  LDD0080  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [4]
AOyne
 Probe Info  		14.70  LDD0443  [5]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C278
 Probe Info  		58.08  LDD1948  [6]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA HEK-293T C59(3.36)  LDD0169  [2]
 LDCM0214  AC1 HEK-293T C59(1.06); C113(0.92)  LDD1507  [7]
 LDCM0215  AC10 HEK-293T C59(1.07)  LDD1508  [7]
 LDCM0226  AC11 HEK-293T C59(1.04)  LDD1509  [7]
 LDCM0237  AC12 HEK-293T C59(1.07)  LDD1510  [7]
 LDCM0259  AC14 HEK-293T C59(1.00)  LDD1512  [7]
 LDCM0270  AC15 HEK-293T C59(1.11)  LDD1513  [7]
 LDCM0276  AC17 HEK-293T C59(0.86); C113(0.88)  LDD1515  [7]
 LDCM0277  AC18 HEK-293T C59(1.06)  LDD1516  [7]
 LDCM0278  AC19 HEK-293T C59(0.92)  LDD1517  [7]
 LDCM0279  AC2 HEK-293T C59(1.00)  LDD1518  [7]
 LDCM0280  AC20 HEK-293T C59(1.09)  LDD1519  [7]
 LDCM0281  AC21 HEK-293T C59(0.92); C113(0.92)  LDD1520  [7]
 LDCM0282  AC22 HEK-293T C59(0.90)  LDD1521  [7]
 LDCM0283  AC23 HEK-293T C59(1.19)  LDD1522  [7]
 LDCM0284  AC24 HEK-293T C113(0.87)  LDD1523  [7]
 LDCM0285  AC25 HEK-293T C59(1.12); C113(0.86)  LDD1524  [7]
 LDCM0286  AC26 HEK-293T C59(1.09)  LDD1525  [7]
 LDCM0287  AC27 HEK-293T C59(0.97)  LDD1526  [7]
 LDCM0288  AC28 HEK-293T C59(0.97)  LDD1527  [7]
 LDCM0289  AC29 HEK-293T C59(0.96); C113(1.01)  LDD1528  [7]
 LDCM0290  AC3 HEK-293T C59(1.10)  LDD1529  [7]
 LDCM0291  AC30 HEK-293T C59(0.96)  LDD1530  [7]
 LDCM0292  AC31 HEK-293T C59(0.99)  LDD1531  [7]
 LDCM0293  AC32 HEK-293T C113(0.89)  LDD1532  [7]
 LDCM0294  AC33 HEK-293T C59(0.93); C113(0.71)  LDD1533  [7]
 LDCM0295  AC34 HEK-293T C59(0.86)  LDD1534  [7]
 LDCM0296  AC35 HEK-293T C59(0.94)  LDD1535  [7]
 LDCM0297  AC36 HEK-293T C59(0.99)  LDD1536  [7]
 LDCM0298  AC37 HEK-293T C59(0.95); C113(1.00)  LDD1537  [7]
 LDCM0299  AC38 HEK-293T C59(1.31)  LDD1538  [7]
 LDCM0300  AC39 HEK-293T C59(1.17)  LDD1539  [7]
 LDCM0301  AC4 HEK-293T C59(1.11)  LDD1540  [7]
 LDCM0302  AC40 HEK-293T C113(0.89)  LDD1541  [7]
 LDCM0303  AC41 HEK-293T C59(1.08); C113(0.89)  LDD1542  [7]
 LDCM0304  AC42 HEK-293T C59(1.01)  LDD1543  [7]
 LDCM0305  AC43 HEK-293T C59(1.09)  LDD1544  [7]
 LDCM0306  AC44 HEK-293T C59(0.90)  LDD1545  [7]
 LDCM0307  AC45 HEK-293T C59(1.05); C113(0.96)  LDD1546  [7]
 LDCM0308  AC46 HEK-293T C59(0.85)  LDD1547  [7]
 LDCM0309  AC47 HEK-293T C59(0.96)  LDD1548  [7]
 LDCM0310  AC48 HEK-293T C113(0.94)  LDD1549  [7]
 LDCM0311  AC49 HEK-293T C59(0.96); C113(0.87)  LDD1550  [7]
 LDCM0312  AC5 HEK-293T C59(1.06); C113(0.93)  LDD1551  [7]
 LDCM0313  AC50 HEK-293T C59(1.09)  LDD1552  [7]
 LDCM0314  AC51 HEK-293T C59(1.08)  LDD1553  [7]
 LDCM0315  AC52 HEK-293T C59(0.92)  LDD1554  [7]
 LDCM0316  AC53 HEK-293T C59(1.00); C113(0.94)  LDD1555  [7]
 LDCM0317  AC54 HEK-293T C59(0.94)  LDD1556  [7]
 LDCM0318  AC55 HEK-293T C59(1.00)  LDD1557  [7]
 LDCM0319  AC56 HEK-293T C113(0.83)  LDD1558  [7]
 LDCM0320  AC57 HEK-293T C59(0.90); C113(1.28)  LDD1559  [7]
 LDCM0321  AC58 HEK-293T C59(1.14)  LDD1560  [7]
 LDCM0322  AC59 HEK-293T C59(1.13)  LDD1561  [7]
 LDCM0323  AC6 HEK-293T C59(0.98)  LDD1562  [7]
 LDCM0324  AC60 HEK-293T C59(1.06)  LDD1563  [7]
 LDCM0325  AC61 HEK-293T C59(1.00); C113(0.92)  LDD1564  [7]
 LDCM0326  AC62 HEK-293T C59(0.79)  LDD1565  [7]
 LDCM0327  AC63 HEK-293T C59(1.01)  LDD1566  [7]
 LDCM0328  AC64 HEK-293T C113(0.80)  LDD1567  [7]
 LDCM0334  AC7 HEK-293T C59(1.02)  LDD1568  [7]
 LDCM0345  AC8 HEK-293T C113(0.97)  LDD1569  [7]
 LDCM0248  AKOS034007472 HEK-293T C59(0.89); C113(1.04)  LDD1511  [7]
 LDCM0356  AKOS034007680 HEK-293T C59(1.03); C113(1.21)  LDD1570  [7]
 LDCM0275  AKOS034007705 HEK-293T C113(0.95)  LDD1514  [7]
 LDCM0630  CCW28-3 231MFP C113(0.84)  LDD2214  [8]
 LDCM0632  CL-Sc Hep-G2 C59(1.50)  LDD2227  [1]
 LDCM0367  CL1 HEK-293T C59(1.05)  LDD1571  [7]
 LDCM0368  CL10 HEK-293T C59(1.07)  LDD1572  [7]
 LDCM0369  CL100 HEK-293T C59(1.15)  LDD1573  [7]
 LDCM0370  CL101 HEK-293T C59(1.21)  LDD1574  [7]
 LDCM0371  CL102 HEK-293T C59(1.02)  LDD1575  [7]
 LDCM0372  CL103 HEK-293T C59(1.00)  LDD1576  [7]
 LDCM0373  CL104 HEK-293T C59(1.04)  LDD1577  [7]
 LDCM0374  CL105 HEK-293T C59(1.23)  LDD1578  [7]
 LDCM0375  CL106 HEK-293T C59(0.94)  LDD1579  [7]
 LDCM0376  CL107 HEK-293T C59(1.05)  LDD1580  [7]
 LDCM0377  CL108 HEK-293T C59(1.18)  LDD1581  [7]
 LDCM0378  CL109 HEK-293T C59(1.02)  LDD1582  [7]
 LDCM0379  CL11 HEK-293T C59(1.20)  LDD1583  [7]
 LDCM0380  CL110 HEK-293T C59(0.94)  LDD1584  [7]
 LDCM0381  CL111 HEK-293T C59(0.98)  LDD1585  [7]
 LDCM0382  CL112 HEK-293T C59(1.11)  LDD1586  [7]
 LDCM0383  CL113 HEK-293T C59(1.00)  LDD1587  [7]
 LDCM0384  CL114 HEK-293T C59(0.90)  LDD1588  [7]
 LDCM0385  CL115 HEK-293T C59(1.09)  LDD1589  [7]
 LDCM0386  CL116 HEK-293T C59(0.98)  LDD1590  [7]
 LDCM0387  CL117 HEK-293T C59(1.45)  LDD1591  [7]
 LDCM0388  CL118 HEK-293T C59(1.02)  LDD1592  [7]
 LDCM0389  CL119 HEK-293T C59(0.97)  LDD1593  [7]
 LDCM0390  CL12 HEK-293T C113(1.47)  LDD1594  [7]
 LDCM0391  CL120 HEK-293T C59(0.95)  LDD1595  [7]
 LDCM0392  CL121 HEK-293T C59(1.28)  LDD1596  [7]
 LDCM0393  CL122 HEK-293T C59(0.99)  LDD1597  [7]
 LDCM0394  CL123 HEK-293T C59(0.86)  LDD1598  [7]
 LDCM0395  CL124 HEK-293T C59(0.94)  LDD1599  [7]
 LDCM0396  CL125 HEK-293T C59(1.09)  LDD1600  [7]
 LDCM0397  CL126 HEK-293T C59(0.93)  LDD1601  [7]
 LDCM0398  CL127 HEK-293T C59(1.00)  LDD1602  [7]
 LDCM0399  CL128 HEK-293T C59(1.03)  LDD1603  [7]
 LDCM0400  CL13 HEK-293T C59(1.21)  LDD1604  [7]
 LDCM0401  CL14 HEK-293T C59(1.12)  LDD1605  [7]
 LDCM0402  CL15 HEK-293T C59(1.12)  LDD1606  [7]
 LDCM0403  CL16 HEK-293T C59(1.10)  LDD1607  [7]
 LDCM0404  CL17 HEK-293T C59(1.09); C113(0.72)  LDD1608  [7]
 LDCM0405  CL18 HEK-293T C59(1.05)  LDD1609  [7]
 LDCM0406  CL19 HEK-293T C59(0.92)  LDD1610  [7]
 LDCM0407  CL2 HEK-293T C59(1.12)  LDD1611  [7]
 LDCM0408  CL20 HEK-293T C59(1.16)  LDD1612  [7]
 LDCM0409  CL21 HEK-293T C59(0.84); C113(0.89)  LDD1613  [7]
 LDCM0410  CL22 HEK-293T C59(0.93)  LDD1614  [7]
 LDCM0411  CL23 HEK-293T C59(1.22)  LDD1615  [7]
 LDCM0412  CL24 HEK-293T C113(1.45)  LDD1616  [7]
 LDCM0413  CL25 HEK-293T C59(1.01)  LDD1617  [7]
 LDCM0414  CL26 HEK-293T C59(0.97)  LDD1618  [7]
 LDCM0415  CL27 HEK-293T C59(1.15)  LDD1619  [7]
 LDCM0416  CL28 HEK-293T C59(0.98)  LDD1620  [7]
 LDCM0417  CL29 HEK-293T C59(0.94); C113(1.13)  LDD1621  [7]
 LDCM0418  CL3 HEK-293T C59(1.23)  LDD1622  [7]
 LDCM0419  CL30 HEK-293T C59(0.92)  LDD1623  [7]
 LDCM0420  CL31 HEK-293T C59(1.08)  LDD1624  [7]
 LDCM0421  CL32 HEK-293T C59(0.91)  LDD1625  [7]
 LDCM0422  CL33 HEK-293T C59(0.77); C113(0.97)  LDD1626  [7]
 LDCM0423  CL34 HEK-293T C59(1.05)  LDD1627  [7]
 LDCM0424  CL35 HEK-293T C59(1.20)  LDD1628  [7]
 LDCM0425  CL36 HEK-293T C113(0.94)  LDD1629  [7]
 LDCM0426  CL37 HEK-293T C59(1.10)  LDD1630  [7]
 LDCM0428  CL39 HEK-293T C59(1.54)  LDD1632  [7]
 LDCM0429  CL4 HEK-293T C59(1.18)  LDD1633  [7]
 LDCM0430  CL40 HEK-293T C59(1.01)  LDD1634  [7]
 LDCM0431  CL41 HEK-293T C59(0.80); C113(0.74)  LDD1635  [7]
 LDCM0432  CL42 HEK-293T C59(0.97)  LDD1636  [7]
 LDCM0433  CL43 HEK-293T C59(1.02)  LDD1637  [7]
 LDCM0434  CL44 HEK-293T C59(0.97)  LDD1638  [7]
 LDCM0435  CL45 HEK-293T C59(0.97); C113(1.28)  LDD1639  [7]
 LDCM0436  CL46 HEK-293T C59(0.80)  LDD1640  [7]
 LDCM0437  CL47 HEK-293T C59(1.01)  LDD1641  [7]
 LDCM0438  CL48 HEK-293T C113(1.05)  LDD1642  [7]
 LDCM0439  CL49 HEK-293T C59(1.11)  LDD1643  [7]
 LDCM0440  CL5 HEK-293T C59(1.16); C113(0.96)  LDD1644  [7]
 LDCM0441  CL50 HEK-293T C59(1.00)  LDD1645  [7]
 LDCM0443  CL52 HEK-293T C59(1.04)  LDD1646  [7]
 LDCM0444  CL53 HEK-293T C59(0.87); C113(0.73)  LDD1647  [7]
 LDCM0445  CL54 HEK-293T C59(0.75)  LDD1648  [7]
 LDCM0446  CL55 HEK-293T C59(1.06)  LDD1649  [7]
 LDCM0447  CL56 HEK-293T C59(1.10)  LDD1650  [7]
 LDCM0448  CL57 HEK-293T C59(0.82); C113(1.09)  LDD1651  [7]
 LDCM0449  CL58 HEK-293T C59(1.55)  LDD1652  [7]
 LDCM0450  CL59 HEK-293T C59(0.99)  LDD1653  [7]
 LDCM0451  CL6 HEK-293T C59(0.99)  LDD1654  [7]
 LDCM0452  CL60 HEK-293T C113(1.14)  LDD1655  [7]
 LDCM0453  CL61 HEK-293T C59(1.32)  LDD1656  [7]
 LDCM0454  CL62 HEK-293T C59(1.17)  LDD1657  [7]
 LDCM0455  CL63 HEK-293T C59(0.97)  LDD1658  [7]
 LDCM0456  CL64 HEK-293T C59(1.29)  LDD1659  [7]
 LDCM0457  CL65 HEK-293T C59(1.08); C113(1.54)  LDD1660  [7]
 LDCM0458  CL66 HEK-293T C59(1.01)  LDD1661  [7]
 LDCM0459  CL67 HEK-293T C59(1.07)  LDD1662  [7]
 LDCM0460  CL68 HEK-293T C59(0.99)  LDD1663  [7]
 LDCM0461  CL69 HEK-293T C59(0.87); C113(1.07)  LDD1664  [7]
 LDCM0462  CL7 HEK-293T C59(0.99)  LDD1665  [7]
 LDCM0463  CL70 HEK-293T C59(1.01)  LDD1666  [7]
 LDCM0464  CL71 HEK-293T C59(1.02)  LDD1667  [7]
 LDCM0465  CL72 HEK-293T C113(1.18)  LDD1668  [7]
 LDCM0466  CL73 HEK-293T C59(0.92)  LDD1669  [7]
 LDCM0467  CL74 HEK-293T C59(1.03)  LDD1670  [7]
 LDCM0469  CL76 HEK-293T C59(1.13)  LDD1672  [7]
 LDCM0470  CL77 HEK-293T C59(1.08); C113(0.97)  LDD1673  [7]
 LDCM0471  CL78 HEK-293T C59(1.00)  LDD1674  [7]
 LDCM0472  CL79 HEK-293T C59(1.08)  LDD1675  [7]
 LDCM0473  CL8 HEK-293T C59(0.91)  LDD1676  [7]
 LDCM0474  CL80 HEK-293T C59(1.08)  LDD1677  [7]
 LDCM0475  CL81 HEK-293T C59(0.91); C113(1.09)  LDD1678  [7]
 LDCM0476  CL82 HEK-293T C59(1.34)  LDD1679  [7]
 LDCM0477  CL83 HEK-293T C59(1.15)  LDD1680  [7]
 LDCM0478  CL84 HEK-293T C113(0.83)  LDD1681  [7]
 LDCM0479  CL85 HEK-293T C59(0.99)  LDD1682  [7]
 LDCM0480  CL86 HEK-293T C59(1.29)  LDD1683  [7]
 LDCM0481  CL87 HEK-293T C59(1.17)  LDD1684  [7]
 LDCM0482  CL88 HEK-293T C59(1.15)  LDD1685  [7]
 LDCM0483  CL89 HEK-293T C59(0.95); C113(0.81)  LDD1686  [7]
 LDCM0484  CL9 HEK-293T C59(1.01); C113(0.92)  LDD1687  [7]
 LDCM0485  CL90 HEK-293T C59(0.87)  LDD1688  [7]
 LDCM0486  CL91 HEK-293T C59(1.10)  LDD1689  [7]
 LDCM0487  CL92 HEK-293T C59(1.00)  LDD1690  [7]
 LDCM0488  CL93 HEK-293T C59(1.02); C113(1.00)  LDD1691  [7]
 LDCM0489  CL94 HEK-293T C59(1.17)  LDD1692  [7]
 LDCM0490  CL95 HEK-293T C59(1.05)  LDD1693  [7]
 LDCM0491  CL96 HEK-293T C113(0.87)  LDD1694  [7]
 LDCM0492  CL97 HEK-293T C59(1.03)  LDD1695  [7]
 LDCM0493  CL98 HEK-293T C59(1.23)  LDD1696  [7]
 LDCM0494  CL99 HEK-293T C59(2.06)  LDD1697  [7]
 LDCM0495  E2913 HEK-293T C59(1.59)  LDD1698  [7]
 LDCM0468  Fragment33 HEK-293T C59(1.19)  LDD1671  [7]
 LDCM0427  Fragment51 HEK-293T C59(1.01)  LDD1631  [7]
 LDCM0022  KB02 HCT 116 C59(1.71)  LDD0080  [3]
 LDCM0023  KB03 HCT 116 C59(1.24)  LDD0081  [3]
 LDCM0024  KB05 HCT 116 C59(1.35)  LDD0082  [3]

References

1 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
7 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
8 Covalent Ligand Screening Uncovers a RNF4 E3 Ligase Recruiter for Targeted Protein Degradation Applications. ACS Chem Biol. 2019 Nov 15;14(11):2430-2440. doi: 10.1021/acschembio.8b01083. Epub 2019 May 13.