Details of the Target

General Information of Target

Target ID LDTP15282
Target Name tRNA 2'-phosphotransferase 1 (TRPT1)
Gene Name TRPT1
Gene ID 83707
Synonyms
tRNA 2'-phosphotransferase 1; EC 2.7.1.160
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MVTCAHLGRRARLPAAQPSACPGTCFSQEERMAAGYLPRWSQELVTFEDVSMDFSQEEWE
LLEPAQKNLYREVMLENYRNVVSLEALKNQCTDVGIKEGPLSPAQTSQVTSLSSWTGYLL
FQPVASSHLEQREALWIEEKGTPQASCSDWMTVLRNQDSTYKKVALQEEPASGINMIKLI
REDGGWKQLEDSHEDPQGLLSQKASLHVVAVPQEKATAWHGFGENGNLSPALVLSQGSSK
GNHLCGSELDITSLASDSVLNHHQLGYADRRPCESNECGNAIRQNSHFIQHGGKMFVYLE
NGQSLNHGMALTIHNKINTAEKPFECHQCGKVFNRRHSLSEHQRIHTGEKPYECQECGRA
FTHSSTLTRHLRTHTGEKPYGCGECGKAFNRISSLTQHQRIHTGEKPYKCEDCGKSFCQS
SYLILHKRTHTGEKPYECSECGKAFSDRSSLNQHERTHTGENPYECKQCGRAFSQRSSLV
RHERTHTGEKPYRCQECGKAFSQSSSLVTHQKTHSSQKTYKIIDCGKAFYQNRHLIGY
Target Bioclass
Enzyme
Family
KptA/TPT1 family
Function Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.
Uniprot ID
Q86TN4
Ensemble ID
ENST00000317459.11
HGNC ID
HGNC:20316

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C98(1.23)  LDD3313  [1]
AHL-Pu-1
 Probe Info  		C237(3.91)  LDD0171  [2]
IPM
 Probe Info  		C237(0.92)  LDD1702  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		C237(0.00); C173(0.00)  LDD0038  [4]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [4]
Lodoacetamide azide
 Probe Info  		C173(0.00); C237(0.00)  LDD0037  [4]
NAIA_4
 Probe Info  		N.A.  LDD2226  [5]
NAIA_5
 Probe Info  		N.A.  LDD2225  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0026  4SU-RNA+native RNA DM93 C237(3.91)  LDD0171  [2]
 LDCM0226  AC11 HEK-293T C237(1.07)  LDD1509  [6]
 LDCM0237  AC12 HEK-293T C173(1.07)  LDD1510  [6]
 LDCM0259  AC14 HEK-293T C237(1.07); C147(0.93)  LDD1512  [6]
 LDCM0270  AC15 HEK-293T C173(1.00); C237(1.01); C147(0.95)  LDD1513  [6]
 LDCM0278  AC19 HEK-293T C237(1.26)  LDD1517  [6]
 LDCM0280  AC20 HEK-293T C173(0.90)  LDD1519  [6]
 LDCM0281  AC21 HEK-293T C173(0.99); C147(0.94)  LDD1520  [6]
 LDCM0282  AC22 HEK-293T C237(1.06); C147(0.92)  LDD1521  [6]
 LDCM0283  AC23 HEK-293T C173(0.97); C237(1.08); C147(0.99)  LDD1522  [6]
 LDCM0284  AC24 HEK-293T C173(0.92); C147(0.91)  LDD1523  [6]
 LDCM0287  AC27 HEK-293T C237(1.12)  LDD1526  [6]
 LDCM0288  AC28 HEK-293T C173(0.93)  LDD1527  [6]
 LDCM0289  AC29 HEK-293T C173(0.93); C147(0.92)  LDD1528  [6]
 LDCM0290  AC3 HEK-293T C237(1.15)  LDD1529  [6]
 LDCM0291  AC30 HEK-293T C237(0.91); C147(1.03)  LDD1530  [6]
 LDCM0292  AC31 HEK-293T C173(0.92); C237(0.84); C147(1.11)  LDD1531  [6]
 LDCM0293  AC32 HEK-293T C173(1.10); C147(0.90)  LDD1532  [6]
 LDCM0296  AC35 HEK-293T C237(1.11)  LDD1535  [6]
 LDCM0297  AC36 HEK-293T C173(0.89)  LDD1536  [6]
 LDCM0298  AC37 HEK-293T C173(1.04); C147(0.96)  LDD1537  [6]
 LDCM0299  AC38 HEK-293T C237(1.17); C147(0.93)  LDD1538  [6]
 LDCM0300  AC39 HEK-293T C173(1.27); C237(1.01); C147(1.14)  LDD1539  [6]
 LDCM0301  AC4 HEK-293T C173(0.99)  LDD1540  [6]
 LDCM0302  AC40 HEK-293T C173(1.13); C147(0.97)  LDD1541  [6]
 LDCM0305  AC43 HEK-293T C237(1.24)  LDD1544  [6]
 LDCM0306  AC44 HEK-293T C173(1.19)  LDD1545  [6]
 LDCM0307  AC45 HEK-293T C173(0.99); C147(0.92)  LDD1546  [6]
 LDCM0308  AC46 HEK-293T C237(1.02); C147(0.92)  LDD1547  [6]
 LDCM0309  AC47 HEK-293T C173(0.90); C237(0.83); C147(1.29)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C173(0.96); C147(0.89)  LDD1549  [6]
 LDCM0312  AC5 HEK-293T C173(1.38); C147(0.98)  LDD1551  [6]
 LDCM0314  AC51 HEK-293T C237(1.15)  LDD1553  [6]
 LDCM0315  AC52 HEK-293T C173(0.93)  LDD1554  [6]
 LDCM0316  AC53 HEK-293T C173(1.35); C147(0.96)  LDD1555  [6]
 LDCM0317  AC54 HEK-293T C237(1.05); C147(0.87)  LDD1556  [6]
 LDCM0318  AC55 HEK-293T C173(1.22); C237(0.98); C147(0.99)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C173(0.98); C147(0.96)  LDD1558  [6]
 LDCM0322  AC59 HEK-293T C237(1.02)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C237(1.03); C147(1.02)  LDD1562  [6]
 LDCM0324  AC60 HEK-293T C173(0.95)  LDD1563  [6]
 LDCM0325  AC61 HEK-293T C173(1.12); C147(0.98)  LDD1564  [6]
 LDCM0326  AC62 HEK-293T C237(1.04); C147(0.99)  LDD1565  [6]
 LDCM0327  AC63 HEK-293T C173(1.21); C237(0.88); C147(0.96)  LDD1566  [6]
 LDCM0328  AC64 HEK-293T C173(0.97); C147(0.91)  LDD1567  [6]
 LDCM0334  AC7 HEK-293T C173(1.04); C237(1.09); C147(0.83)  LDD1568  [6]
 LDCM0345  AC8 HEK-293T C173(0.85); C147(1.03)  LDD1569  [6]
 LDCM0248  AKOS034007472 HEK-293T C173(1.14); C147(1.00)  LDD1511  [6]
 LDCM0275  AKOS034007705 HEK-293T C173(0.99); C147(0.94)  LDD1514  [6]
 LDCM0367  CL1 HEK-293T C173(0.76)  LDD1571  [6]
 LDCM0368  CL10 HEK-293T C237(0.79); C147(0.86)  LDD1572  [6]
 LDCM0369  CL100 HEK-293T C173(0.72)  LDD1573  [6]
 LDCM0370  CL101 HEK-293T C173(0.66)  LDD1574  [6]
 LDCM0372  CL103 HEK-293T C173(0.99)  LDD1576  [6]
 LDCM0373  CL104 HEK-293T C173(0.68)  LDD1577  [6]
 LDCM0374  CL105 HEK-293T C173(1.24)  LDD1578  [6]
 LDCM0376  CL107 HEK-293T C173(0.94)  LDD1580  [6]
 LDCM0377  CL108 HEK-293T C173(0.86)  LDD1581  [6]
 LDCM0378  CL109 HEK-293T C173(1.04)  LDD1582  [6]
 LDCM0379  CL11 HEK-293T C173(0.99); C237(0.73); C147(0.88)  LDD1583  [6]
 LDCM0381  CL111 HEK-293T C173(1.04)  LDD1585  [6]
 LDCM0382  CL112 HEK-293T C173(0.12)  LDD1586  [6]
 LDCM0383  CL113 HEK-293T C173(0.95)  LDD1587  [6]
 LDCM0385  CL115 HEK-293T C173(1.06)  LDD1589  [6]
 LDCM0386  CL116 HEK-293T C173(0.60)  LDD1590  [6]
 LDCM0387  CL117 HEK-293T C173(1.14)  LDD1591  [6]
 LDCM0389  CL119 HEK-293T C173(0.86)  LDD1593  [6]
 LDCM0390  CL12 HEK-293T C173(0.97); C147(0.98)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C173(0.82)  LDD1595  [6]
 LDCM0392  CL121 HEK-293T C173(0.94)  LDD1596  [6]
 LDCM0394  CL123 HEK-293T C173(0.77)  LDD1598  [6]
 LDCM0395  CL124 HEK-293T C173(0.74)  LDD1599  [6]
 LDCM0396  CL125 HEK-293T C173(0.79)  LDD1600  [6]
 LDCM0398  CL127 HEK-293T C173(1.32)  LDD1602  [6]
 LDCM0399  CL128 HEK-293T C173(0.87)  LDD1603  [6]
 LDCM0400  CL13 HEK-293T C173(0.68)  LDD1604  [6]
 LDCM0402  CL15 HEK-293T C173(1.20)  LDD1606  [6]
 LDCM0403  CL16 HEK-293T C173(0.74)  LDD1607  [6]
 LDCM0406  CL19 HEK-293T C237(1.00)  LDD1610  [6]
 LDCM0408  CL20 HEK-293T C173(0.81)  LDD1612  [6]
 LDCM0409  CL21 HEK-293T C173(0.90); C147(0.95)  LDD1613  [6]
 LDCM0410  CL22 HEK-293T C237(0.97); C147(0.97)  LDD1614  [6]
 LDCM0411  CL23 HEK-293T C173(1.12); C237(0.78); C147(1.11)  LDD1615  [6]
 LDCM0412  CL24 HEK-293T C173(1.03); C147(1.05)  LDD1616  [6]
 LDCM0413  CL25 HEK-293T C173(0.96)  LDD1617  [6]
 LDCM0415  CL27 HEK-293T C173(1.27)  LDD1619  [6]
 LDCM0416  CL28 HEK-293T C173(0.80)  LDD1620  [6]
 LDCM0418  CL3 HEK-293T C173(1.07)  LDD1622  [6]
 LDCM0420  CL31 HEK-293T C237(1.04)  LDD1624  [6]
 LDCM0421  CL32 HEK-293T C173(0.90)  LDD1625  [6]
 LDCM0422  CL33 HEK-293T C173(1.16); C147(0.96)  LDD1626  [6]
 LDCM0423  CL34 HEK-293T C237(0.92); C147(1.01)  LDD1627  [6]
 LDCM0424  CL35 HEK-293T C173(0.89); C237(0.76); C147(1.24)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C173(0.93); C147(1.19)  LDD1629  [6]
 LDCM0426  CL37 HEK-293T C173(0.74)  LDD1630  [6]
 LDCM0428  CL39 HEK-293T C173(0.97)  LDD1632  [6]
 LDCM0429  CL4 HEK-293T C173(0.87)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C173(0.77)  LDD1634  [6]
 LDCM0433  CL43 HEK-293T C237(1.18)  LDD1637  [6]
 LDCM0434  CL44 HEK-293T C173(1.16)  LDD1638  [6]
 LDCM0435  CL45 HEK-293T C173(0.86); C147(0.88)  LDD1639  [6]
 LDCM0436  CL46 HEK-293T C237(0.84); C147(1.00)  LDD1640  [6]
 LDCM0437  CL47 HEK-293T C173(0.76); C237(0.76); C147(1.23)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C173(0.89); C147(1.06)  LDD1642  [6]
 LDCM0439  CL49 HEK-293T C173(0.93)  LDD1643  [6]
 LDCM0443  CL52 HEK-293T C173(1.16)  LDD1646  [6]
 LDCM0446  CL55 HEK-293T C237(1.14)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C173(0.89)  LDD1650  [6]
 LDCM0448  CL57 HEK-293T C173(1.03); C147(1.01)  LDD1651  [6]
 LDCM0449  CL58 HEK-293T C237(0.93); C147(1.00)  LDD1652  [6]
 LDCM0450  CL59 HEK-293T C173(1.10); C237(0.75); C147(1.13)  LDD1653  [6]
 LDCM0452  CL60 HEK-293T C173(0.90); C147(1.15)  LDD1655  [6]
 LDCM0453  CL61 HEK-293T C173(0.91)  LDD1656  [6]
 LDCM0455  CL63 HEK-293T C173(1.18)  LDD1658  [6]
 LDCM0456  CL64 HEK-293T C173(0.96)  LDD1659  [6]
 LDCM0459  CL67 HEK-293T C237(1.00)  LDD1662  [6]
 LDCM0460  CL68 HEK-293T C173(0.83)  LDD1663  [6]
 LDCM0461  CL69 HEK-293T C173(1.25); C147(0.94)  LDD1664  [6]
 LDCM0462  CL7 HEK-293T C237(0.92)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C237(0.93); C147(1.03)  LDD1666  [6]
 LDCM0464  CL71 HEK-293T C173(0.99); C237(0.72); C147(0.96)  LDD1667  [6]
 LDCM0465  CL72 HEK-293T C173(1.01); C147(1.04)  LDD1668  [6]
 LDCM0466  CL73 HEK-293T C173(0.89)  LDD1669  [6]
 LDCM0469  CL76 HEK-293T C173(0.86)  LDD1672  [6]
 LDCM0472  CL79 HEK-293T C237(0.99)  LDD1675  [6]
 LDCM0473  CL8 HEK-293T C173(0.74)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C173(1.02)  LDD1677  [6]
 LDCM0475  CL81 HEK-293T C173(0.92); C147(0.95)  LDD1678  [6]
 LDCM0476  CL82 HEK-293T C237(0.94); C147(0.98)  LDD1679  [6]
 LDCM0477  CL83 HEK-293T C173(0.87); C237(0.79); C147(1.11)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C173(1.08); C147(1.06)  LDD1681  [6]
 LDCM0479  CL85 HEK-293T C173(0.64)  LDD1682  [6]
 LDCM0481  CL87 HEK-293T C173(1.02)  LDD1684  [6]
 LDCM0482  CL88 HEK-293T C173(0.91)  LDD1685  [6]
 LDCM0484  CL9 HEK-293T C173(1.00); C147(0.89)  LDD1687  [6]
 LDCM0486  CL91 HEK-293T C237(0.99)  LDD1689  [6]
 LDCM0487  CL92 HEK-293T C173(0.84)  LDD1690  [6]
 LDCM0488  CL93 HEK-293T C173(1.04); C147(0.93)  LDD1691  [6]
 LDCM0489  CL94 HEK-293T C237(0.97); C147(1.02)  LDD1692  [6]
 LDCM0490  CL95 HEK-293T C173(0.79); C237(0.72); C147(1.28)  LDD1693  [6]
 LDCM0491  CL96 HEK-293T C173(1.10); C147(0.96)  LDD1694  [6]
 LDCM0492  CL97 HEK-293T C173(0.72)  LDD1695  [6]
 LDCM0494  CL99 HEK-293T C173(0.81)  LDD1697  [6]
 LDCM0495  E2913 HEK-293T C173(1.33)  LDD1698  [6]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C237(0.92)  LDD1702  [3]
 LDCM0625  F8 Ramos C237(1.13)  LDD2187  [7]
 LDCM0572  Fragment10 Ramos C237(1.79)  LDD2189  [7]
 LDCM0573  Fragment11 Ramos C237(2.48)  LDD2190  [7]
 LDCM0574  Fragment12 Ramos C237(0.97)  LDD2191  [7]
 LDCM0575  Fragment13 Ramos C237(1.10)  LDD2192  [7]
 LDCM0576  Fragment14 Ramos C237(0.94)  LDD2193  [7]
 LDCM0579  Fragment20 Ramos C237(0.82)  LDD2194  [7]
 LDCM0580  Fragment21 Ramos C237(1.50)  LDD2195  [7]
 LDCM0582  Fragment23 Ramos C237(1.63)  LDD2196  [7]
 LDCM0578  Fragment27 Ramos C237(1.24)  LDD2197  [7]
 LDCM0586  Fragment28 Ramos C237(0.87)  LDD2198  [7]
 LDCM0588  Fragment30 Ramos C237(1.32)  LDD2199  [7]
 LDCM0589  Fragment31 Ramos C237(1.09)  LDD2200  [7]
 LDCM0590  Fragment32 Ramos C237(1.35)  LDD2201  [7]
 LDCM0468  Fragment33 HEK-293T C173(1.13)  LDD1671  [6]
 LDCM0596  Fragment38 Ramos C237(1.23)  LDD2203  [7]
 LDCM0566  Fragment4 Ramos C237(0.88)  LDD2184  [7]
 LDCM0610  Fragment52 Ramos C237(1.39)  LDD2204  [7]
 LDCM0614  Fragment56 Ramos C237(1.36)  LDD2205  [7]
 LDCM0569  Fragment7 Ramos C237(1.21)  LDD2186  [7]
 LDCM0571  Fragment9 Ramos C237(1.18)  LDD2188  [7]
 LDCM0022  KB02 HEK-293T C237(0.98); C173(1.05)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C237(0.93); C173(0.84)  LDD1497  [6]
 LDCM0024  KB05 Hs 936.T C98(1.23)  LDD3313  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
TNF receptor-associated factor 2 (TRAF2) TNF receptor-associated factor family Q12933
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Golgi reassembly-stacking protein 2 (GORASP2) GORASP family Q9H8Y8

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
7 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578