Details of the Target

General Information of Target

Target ID LDTP14411
Target Name Nascent polypeptide-associated complex subunit alpha, muscle-specific form (NACA)
Gene Name NACA
Synonyms
Nascent polypeptide-associated complex subunit alpha, muscle-specific form; Alpha-NAC, muscle-specific form; skNAC
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MASRGALRRCLSPGLPRLLHLSRGLA
Target Bioclass
Transcription factor
Subcellular location
Cytoplasm
Function
Cardiac- and muscle-specific transcription factor. May act to regulate the expression of genes involved in the development of myotubes. Plays a critical role in ventricular cardiomyocyte expansion and regulates postnatal skeletal muscle growth and regeneration. Involved in the organized assembly of thick and thin filaments of myofibril sarcomeres.
Uniprot ID
E9PAV3
Ensemble ID
ENST00000454682.6
HGNC ID
HGNC:7629

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A375 SNV: p.P649S .
COLO792 SNV: p.P1343S; p.P1503L .
DU145 SNV: p.H215L .
G361 SNV: p.P131L .
HCT116 SNV: p.V1858I .
HCT15 SNV: p.T902I .
HDMYZ SNV: p.L158H .
HEP3B217 Deletion: p.P1255_G1277del .
HT115 SNV: p.P471L; p.P561S .
HUH7 SNV: p.P1082H .
Ishikawa (Heraklio) 02 ER Deletion: p.P299QfsTer25; p.P1336QfsTer3 .
JURKAT Deletion: p.P299QfsTer25;p.P959RfsTer56
SNV: p.G1851R;p.G1987E		 .
KASUMI1 SNV: p.P1320Q .
KYSE180 SNV: p.P649S .
MCC13 SNV: p.P1435L .
MEWO SNV: p.P1808L .
MFE319 SNV: p.T552A; p.P1622L .
MM1S Substitution: p.P1255L .
MOLT4 Deletion: p.G1045EfsTer73;p.P1551QfsTer3
SNV: p.P81H;p.T211I;p.S520G;p.P720A;p.L721M;p.P996H;p.P1065L;p.A1552G		 .
NCIH1944 SNV: p.G816C .
OAW28 SNV: p.P1320A .
REH SNV: p.A1383V .
RL952 SNV: p.S1650C .
SW403 SNV: p.P1801H .
WM2664 SNV: p.P1053S .
WM793 SNV: p.A1632V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K1945(7.75); K1963(10.00); K1971(10.00); K2005(10.00)  LDD0277  [1]
AZ-9
 Probe Info  		E2004(1.00); E2045(0.86); E1988(0.95)  LDD2208  [2]
ONAyne
 Probe Info  		N.A.  LDD0273  [1]
P11
 Probe Info  		5.69  LDD0201  [3]
DBIA
 Probe Info  		C1717(2.42)  LDD3401  [4]
HHS-482
 Probe Info  		Y1975(0.09)  LDD0287  [5]
HHS-475
 Probe Info  		Y1975(0.84); Y1983(0.94)  LDD0264  [6]
HHS-465
 Probe Info  		Y1983(10.00)  LDD2237  [7]
1c-yne
 Probe Info  		K1963(0.00); K1971(0.00); K1976(0.00); K1990(0.00)  LDD0228  [8]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Photonaproxen
 Probe Info  		I1991(0.00); L1994(0.00)  LDD0156  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0088  C45 HEK-293T 5.69  LDD0201  [3]
 LDCM0116  HHS-0101 DM93 Y1975(0.84); Y1983(0.94)  LDD0264  [6]
 LDCM0117  HHS-0201 DM93 Y1975(0.71); Y1983(1.06)  LDD0265  [6]
 LDCM0118  HHS-0301 DM93 Y1975(0.90); Y1983(1.32)  LDD0266  [6]
 LDCM0119  HHS-0401 DM93 Y1975(0.84); Y1983(1.29)  LDD0267  [6]
 LDCM0120  HHS-0701 DM93 Y1975(0.74); Y1983(0.98)  LDD0268  [6]
 LDCM0125  JWB146 DM93 Y1975(0.09)  LDD0287  [5]
 LDCM0126  JWB150 DM93 Y1975(0.14)  LDD0288  [5]
 LDCM0127  JWB152 DM93 Y1975(0.26)  LDD0289  [5]
 LDCM0128  JWB198 DM93 Y1975(0.07)  LDD0290  [5]
 LDCM0129  JWB202 DM93 Y1975(0.09)  LDD0291  [5]
 LDCM0130  JWB211 DM93 Y1975(0.18)  LDD0292  [5]
 LDCM0022  KB02 RD C1717(1.77)  LDD2567  [4]
 LDCM0023  KB03 RD C1717(1.66)  LDD2984  [4]
 LDCM0024  KB05 RD C1717(2.42)  LDD3401  [4]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
3 Discovery of Potent and Selective Inhibitors against Protein-Derived Electrophilic Cofactors. J Am Chem Soc. 2022 Mar 30;144(12):5377-5388. doi: 10.1021/jacs.1c12748. Epub 2022 Mar 2.
4 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
5 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
6 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
7 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
8 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
9 Small Molecule Interactome Mapping by Photoaffinity Labeling Reveals Binding Site Hotspots for the NSAIDs. J Am Chem Soc. 2018 Mar 28;140(12):4259-4268. doi: 10.1021/jacs.7b11639. Epub 2018 Mar 15.
Mass spectrometry data entry: PXD007094