Details of the Target

General Information of Target

Target ID LDTP13261
Target Name AF4/FMR2 family member 4 (AFF4)
Gene Name AFF4
Gene ID 27125
Synonyms
AF5Q31; MCEF; AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Protein AF-5q31; Major CDK9 elongation factor-associated protein
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MESSPFNRRQWTSLSLRVTAKELSLVNKNKSSAIVEIFSKYQKAAEETNMEKKRSNTENL
SQHFRKGTLTVLKKKWENPGLGAESHTDSLRNSSTEIRHRADHPPAEVTSHAASGAKADQ
EEQIHPRSRLRSPPEALVQGRYPHIKDGEDLKDHSTESKKMENCLGESRHEVEKSEISEN
TDASGKIEKYNVPLNRLKMMFEKGEPTQTKILRAQSRSASGRKISENSYSLDDLEIGPGQ
LSSSTFDSEKNESRRNLELPRLSETSIKDRMAKYQAAVSKQSSSTNYTNELKASGGEIKI
HKMEQKENVPPGPEVCITHQEGEKISANENSLAVRSTPAEDDSRDSQVKSEVQQPVHPKP
LSPDSRASSLSESSPPKAMKKFQAPARETCVECQKTVYPMERLLANQQVFHISCFRCSYC
NNKLSLGTYASLHGRIYCKPHFNQLFKSKGNYDEGFGHRPHKDLWASKNENEEILERPAQ
LANARETPHSPGVEDAPIAKVGVLAASMEAKASSQQEKEDKPAETKKLRIAWPPPTELGS
SGSALEEGIKMSKPKWPPEDEISKPEVPEDVDLDLKKLRRSSSLKERSRPFTVAASFQST
SVKSPKTVSPPIRKGWSMSEQSEESVGGRVAERKQVENAKASKKNGNVGKTTWQNKESKG
ETGKRSKEGHSLEMENENLVENGADSDEDDNSFLKQQSPQEPKSLNWSSFVDNTFAEEFT
TQNQKSQDVELWEGEVVKELSVEEQIKRNRYYDEDEDEE
Target Bioclass
Transcription factor
Family
AF4 family
Subcellular location
Nucleus
Function
Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
Uniprot ID
Q9UHB7
Ensemble ID
ENST00000265343.10
HGNC ID
HGNC:17869

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CAL148 SNV: p.Q208K .
CCK81 Deletion: p.K569RfsTer14 .
HCT116 Deletion: p.K924SfsTer2 .
HUH28 SNV: p.S814F DBIA    Probe Info 
IGR1 SNV: p.K614R DBIA    Probe Info 
JHH4 SNV: p.K40R DBIA    Probe Info 
JURKAT SNV: p.A138V; p.P891L .
MEWO Insertion: p.R507LfsTer8 .
MOLT4 SNV: p.A1093T .
NCIH1793 SNV: p.K958R .
NCIH82 SNV: p.Q566P .
REH SNV: p.Y156Ter .
RKO Insertion: p.T826NfsTer11 .
SHP77 SNV: p.S795F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 13 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		7.37  LDD0066  [1]
Probe 1
 Probe Info  		Y515(18.77)  LDD3495  [2]
BTD
 Probe Info  		C1002(0.73)  LDD2117  [3]
AHL-Pu-1
 Probe Info  		C315(2.17)  LDD0169  [4]
DBIA
 Probe Info  		C315(1.08)  LDD0078  [5]
m-APA
 Probe Info  		N.A.  LDD2231  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [7]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [8]
NAIA_4
 Probe Info  		N.A.  LDD2226  [9]
IPM
 Probe Info  		C315(0.00); C889(0.00)  LDD0147  [10]
VSF
 Probe Info  		N.A.  LDD0007  [11]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [12]
AOyne
 Probe Info  		12.20  LDD0443  [13]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C1002(0.73)  LDD2117  [3]
 LDCM0026  4SU-RNA+native RNA HEK-293T C315(2.17)  LDD0169  [4]
 LDCM0214  AC1 HEK-293T C889(1.04); C1005(0.96); C315(0.85); C338(1.12)  LDD1507  [14]
 LDCM0215  AC10 HEK-293T C889(0.99); C1005(1.05); C315(1.02); C338(1.04)  LDD1508  [14]
 LDCM0226  AC11 HEK-293T C889(0.98); C1005(1.05); C315(1.04); C338(1.09)  LDD1509  [14]
 LDCM0237  AC12 HEK-293T C889(1.06); C1005(1.06); C315(1.00); C338(1.03)  LDD1510  [14]
 LDCM0259  AC14 HEK-293T C889(0.88); C1005(1.11); C315(1.00); C338(1.04)  LDD1512  [14]
 LDCM0270  AC15 HEK-293T C889(1.15); C1005(1.14); C315(1.03); C338(0.99)  LDD1513  [14]
 LDCM0276  AC17 HEK-293T C889(0.98); C1005(1.04); C315(0.90); C338(1.05)  LDD1515  [14]
 LDCM0277  AC18 HEK-293T C889(1.04); C1005(0.95); C315(0.97); C338(0.97)  LDD1516  [14]
 LDCM0278  AC19 HEK-293T C889(1.23); C1005(0.98); C315(1.24); C338(1.54)  LDD1517  [14]
 LDCM0279  AC2 HEK-293T C889(1.02); C1005(0.92); C315(0.95); C338(0.95)  LDD1518  [14]
 LDCM0280  AC20 HEK-293T C889(1.16); C1005(1.00); C315(0.92); C338(0.99)  LDD1519  [14]
 LDCM0281  AC21 HEK-293T C889(1.08); C1005(1.00); C315(0.98); C338(1.06)  LDD1520  [14]
 LDCM0282  AC22 HEK-293T C889(0.90); C1005(1.06); C315(0.96); C338(1.07)  LDD1521  [14]
 LDCM0283  AC23 HEK-293T C889(1.19); C1005(1.08); C315(1.02); C338(0.99)  LDD1522  [14]
 LDCM0284  AC24 HEK-293T C889(0.99); C1005(1.13); C315(0.96); C338(1.01)  LDD1523  [14]
 LDCM0285  AC25 HEK-293T C889(1.01); C1005(1.20); C315(0.94); C338(1.13)  LDD1524  [14]
 LDCM0286  AC26 HEK-293T C889(0.99); C1005(1.02); C315(1.06); C338(1.01)  LDD1525  [14]
 LDCM0287  AC27 HEK-293T C889(0.80); C1005(1.01); C315(1.06); C338(1.05)  LDD1526  [14]
 LDCM0288  AC28 HEK-293T C889(1.02); C1005(1.10); C315(0.98); C338(1.03)  LDD1527  [14]
 LDCM0289  AC29 HEK-293T C889(1.07); C1005(1.06); C315(0.90); C338(1.03)  LDD1528  [14]
 LDCM0290  AC3 HEK-293T C889(0.94); C1005(0.94); C315(1.11); C338(1.04)  LDD1529  [14]
 LDCM0291  AC30 HEK-293T C889(0.95); C1005(1.02); C315(0.89); C338(0.98)  LDD1530  [14]
 LDCM0292  AC31 HEK-293T C889(1.07); C1005(1.14); C315(0.93); C338(0.94)  LDD1531  [14]
 LDCM0293  AC32 HEK-293T C889(1.00); C1005(1.16); C315(0.93); C338(1.04)  LDD1532  [14]
 LDCM0294  AC33 HEK-293T C889(0.84); C1005(0.98); C315(0.84); C338(1.13)  LDD1533  [14]
 LDCM0295  AC34 HEK-293T C889(1.08); C1005(1.08); C315(0.95); C338(1.03)  LDD1534  [14]
 LDCM0296  AC35 HEK-293T C889(0.93); C1005(1.09); C315(1.13); C338(1.09)  LDD1535  [14]
 LDCM0297  AC36 HEK-293T C889(1.13); C1005(1.07); C315(1.02); C338(0.97)  LDD1536  [14]
 LDCM0298  AC37 HEK-293T C889(0.96); C1005(1.03); C315(0.94); C338(1.03)  LDD1537  [14]
 LDCM0299  AC38 HEK-293T C889(0.93); C1005(1.13); C315(0.87); C338(1.05)  LDD1538  [14]
 LDCM0300  AC39 HEK-293T C889(1.06); C1005(1.22); C315(1.02); C338(1.01)  LDD1539  [14]
 LDCM0301  AC4 HEK-293T C889(0.99); C1005(0.96); C315(1.00); C338(1.09)  LDD1540  [14]
 LDCM0302  AC40 HEK-293T C889(1.04); C1005(1.11); C315(1.11); C338(1.04)  LDD1541  [14]
 LDCM0303  AC41 HEK-293T C889(0.83); C1005(1.09); C315(1.00); C338(1.04)  LDD1542  [14]
 LDCM0304  AC42 HEK-293T C889(0.97); C1005(1.01); C315(1.08); C338(1.04)  LDD1543  [14]
 LDCM0305  AC43 HEK-293T C889(0.99); C1005(1.17); C315(1.10); C338(1.15)  LDD1544  [14]
 LDCM0306  AC44 HEK-293T C889(1.09); C1005(1.20); C315(0.98); C338(0.93)  LDD1545  [14]
 LDCM0307  AC45 HEK-293T C889(1.06); C1005(1.08); C315(0.99); C338(1.09)  LDD1546  [14]
 LDCM0308  AC46 HEK-293T C889(0.97); C1005(1.06); C315(0.97); C338(1.15)  LDD1547  [14]
 LDCM0309  AC47 HEK-293T C889(1.18); C1005(1.25); C315(1.02); C338(0.96)  LDD1548  [14]
 LDCM0310  AC48 HEK-293T C889(1.02); C1005(1.22); C315(1.23); C338(1.01)  LDD1549  [14]
 LDCM0311  AC49 HEK-293T C889(0.94); C1005(1.04); C315(0.90); C338(1.06)  LDD1550  [14]
 LDCM0312  AC5 HEK-293T C889(1.04); C1005(1.05); C315(1.05); C338(1.00)  LDD1551  [14]
 LDCM0313  AC50 HEK-293T C889(0.98); C1005(1.11); C315(1.03); C338(1.03)  LDD1552  [14]
 LDCM0314  AC51 HEK-293T C889(0.90); C1005(1.08); C315(1.08); C338(1.04)  LDD1553  [14]
 LDCM0315  AC52 HEK-293T C889(1.12); C1005(1.10); C315(0.98); C338(1.08)  LDD1554  [14]
 LDCM0316  AC53 HEK-293T C889(0.96); C1005(1.09); C315(0.97); C338(1.02)  LDD1555  [14]
 LDCM0317  AC54 HEK-293T C889(0.94); C1005(1.15); C315(0.92); C338(1.03)  LDD1556  [14]
 LDCM0318  AC55 HEK-293T C889(1.08); C1005(1.19); C315(1.01); C338(1.02)  LDD1557  [14]
 LDCM0319  AC56 HEK-293T C889(1.06); C1005(1.17); C315(0.99); C338(1.03)  LDD1558  [14]
 LDCM0320  AC57 HEK-293T C889(0.85); C1005(0.99); C315(0.91); C338(1.18)  LDD1559  [14]
 LDCM0321  AC58 HEK-293T C889(1.03); C1005(0.96); C315(1.04); C338(1.10)  LDD1560  [14]
 LDCM0322  AC59 HEK-293T C889(0.95); C1005(0.99); C315(1.08); C338(1.11)  LDD1561  [14]
 LDCM0323  AC6 HEK-293T C889(0.88); C1005(1.02); C315(0.99); C338(1.07)  LDD1562  [14]
 LDCM0324  AC60 HEK-293T C889(0.97); C1005(1.00); C315(0.95); C338(1.05)  LDD1563  [14]
 LDCM0325  AC61 HEK-293T C889(1.07); C1005(0.98); C315(1.00); C338(1.06)  LDD1564  [14]
 LDCM0326  AC62 HEK-293T C889(0.98); C1005(1.09); C315(1.08); C338(1.01)  LDD1565  [14]
 LDCM0327  AC63 HEK-293T C889(1.14); C1005(1.04); C315(1.03); C338(1.03)  LDD1566  [14]
 LDCM0328  AC64 HEK-293T C889(1.02); C1005(1.08); C315(1.13); C338(0.99)  LDD1567  [14]
 LDCM0334  AC7 HEK-293T C889(1.11); C1005(1.10); C315(1.00); C338(1.00)  LDD1568  [14]
 LDCM0345  AC8 HEK-293T C889(1.04); C1005(1.05); C315(1.17); C338(1.03)  LDD1569  [14]
 LDCM0248  AKOS034007472 HEK-293T C889(1.04); C1005(1.06); C315(1.00); C338(0.93)  LDD1511  [14]
 LDCM0356  AKOS034007680 HEK-293T C889(1.05); C1005(1.05); C315(0.89); C338(0.99)  LDD1570  [14]
 LDCM0275  AKOS034007705 HEK-293T C889(1.05); C1005(1.21); C315(1.01); C338(1.05)  LDD1514  [14]
 LDCM0020  ARS-1620 HCC44 C315(1.08)  LDD0078  [5]
 LDCM0367  CL1 HEK-293T C889(0.97); C1005(0.94); C315(0.92); C338(0.88)  LDD1571  [14]
 LDCM0368  CL10 HEK-293T C889(1.02); C1005(1.29); C315(0.88); C338(1.07)  LDD1572  [14]
 LDCM0369  CL100 HEK-293T C889(0.92); C1005(1.00); C315(1.09); C338(1.06)  LDD1573  [14]
 LDCM0370  CL101 HEK-293T C889(1.01); C1005(1.08); C315(1.07); C338(0.98)  LDD1574  [14]
 LDCM0371  CL102 HEK-293T C889(0.89); C1005(1.16); C315(1.00); C338(1.08)  LDD1575  [14]
 LDCM0372  CL103 HEK-293T C889(1.03); C1005(1.06); C315(1.01); C338(1.05)  LDD1576  [14]
 LDCM0373  CL104 HEK-293T C889(0.79); C1005(1.03); C315(1.07); C338(0.93)  LDD1577  [14]
 LDCM0374  CL105 HEK-293T C889(0.99); C1005(1.07); C315(0.87); C338(0.98)  LDD1578  [14]
 LDCM0375  CL106 HEK-293T C889(0.93); C1005(1.10); C315(1.10); C338(0.86)  LDD1579  [14]
 LDCM0376  CL107 HEK-293T C889(1.17); C1005(1.18); C315(1.02); C338(0.95)  LDD1580  [14]
 LDCM0377  CL108 HEK-293T C889(0.83); C1005(1.01); C315(1.15); C338(1.00)  LDD1581  [14]
 LDCM0378  CL109 HEK-293T C889(0.94); C1005(1.01); C315(1.06); C338(0.96)  LDD1582  [14]
 LDCM0379  CL11 HEK-293T C889(0.93); C1005(1.34); C315(1.01); C338(0.96)  LDD1583  [14]
 LDCM0380  CL110 HEK-293T C889(0.84); C1005(1.02); C315(0.92); C338(1.01)  LDD1584  [14]
 LDCM0381  CL111 HEK-293T C889(1.09); C1005(1.11); C315(1.01); C338(1.05)  LDD1585  [14]
 LDCM0382  CL112 HEK-293T C889(1.01); C1005(1.12); C315(1.03); C338(1.10)  LDD1586  [14]
 LDCM0383  CL113 HEK-293T C889(1.00); C1005(1.00); C315(0.92); C338(0.98)  LDD1587  [14]
 LDCM0384  CL114 HEK-293T C889(0.87); C1005(0.98); C315(1.03); C338(1.11)  LDD1588  [14]
 LDCM0385  CL115 HEK-293T C889(1.07); C1005(0.96); C315(1.03); C338(1.01)  LDD1589  [14]
 LDCM0386  CL116 HEK-293T C889(0.89); C1005(1.12); C315(1.11); C338(0.98)  LDD1590  [14]
 LDCM0387  CL117 HEK-293T C889(1.00); C1005(1.08); C315(1.23); C338(0.94)  LDD1591  [14]
 LDCM0388  CL118 HEK-293T C889(0.97); C1005(1.08); C315(1.06); C338(1.16)  LDD1592  [14]
 LDCM0389  CL119 HEK-293T C889(1.05); C1005(1.13); C315(0.94); C338(1.07)  LDD1593  [14]
 LDCM0390  CL12 HEK-293T C889(0.78); C1005(1.33); C315(0.94); C338(1.02)  LDD1594  [14]
 LDCM0391  CL120 HEK-293T C889(0.98); C1005(1.05); C315(1.05); C338(1.01)  LDD1595  [14]
 LDCM0392  CL121 HEK-293T C889(1.03); C1005(1.11); C315(1.10); C338(1.01)  LDD1596  [14]
 LDCM0393  CL122 HEK-293T C889(0.86); C1005(1.17); C315(1.04); C338(1.03)  LDD1597  [14]
 LDCM0394  CL123 HEK-293T C889(0.93); C1005(1.13); C315(0.95); C338(0.96)  LDD1598  [14]
 LDCM0395  CL124 HEK-293T C889(0.90); C1005(1.18); C315(1.15); C338(1.00)  LDD1599  [14]
 LDCM0396  CL125 HEK-293T C889(1.01); C1005(1.19); C315(0.91); C338(0.97)  LDD1600  [14]
 LDCM0397  CL126 HEK-293T C889(0.91); C1005(1.02); C315(1.06); C338(1.13)  LDD1601  [14]
 LDCM0398  CL127 HEK-293T C889(1.10); C1005(1.05); C315(0.97); C338(0.88)  LDD1602  [14]
 LDCM0399  CL128 HEK-293T C889(0.89); C1005(1.01); C315(1.09); C338(1.03)  LDD1603  [14]
 LDCM0400  CL13 HEK-293T C889(0.99); C1005(1.05); C315(1.08); C338(1.07)  LDD1604  [14]
 LDCM0401  CL14 HEK-293T C889(0.88); C1005(1.13); C315(1.04); C338(1.03)  LDD1605  [14]
 LDCM0402  CL15 HEK-293T C889(1.02); C1005(1.12); C315(1.07); C338(0.96)  LDD1606  [14]
 LDCM0403  CL16 HEK-293T C889(0.83); C1005(1.09); C315(0.95); C338(1.01)  LDD1607  [14]
 LDCM0404  CL17 HEK-293T C889(0.87); C1005(1.13); C315(0.80); C338(1.00)  LDD1608  [14]
 LDCM0405  CL18 HEK-293T C889(1.11); C1005(1.21); C315(0.99); C338(1.00)  LDD1609  [14]
 LDCM0406  CL19 HEK-293T C889(0.91); C1005(1.10); C315(1.25); C338(1.06)  LDD1610  [14]
 LDCM0407  CL2 HEK-293T C889(0.89); C1005(1.05); C315(1.10); C338(1.08)  LDD1611  [14]
 LDCM0408  CL20 HEK-293T C889(1.14); C1005(1.25); C315(0.98); C338(1.03)  LDD1612  [14]
 LDCM0409  CL21 HEK-293T C889(0.89); C1005(1.26); C315(0.95); C338(1.13)  LDD1613  [14]
 LDCM0410  CL22 HEK-293T C889(1.50); C1005(1.38); C315(0.98); C338(1.04)  LDD1614  [14]
 LDCM0411  CL23 HEK-293T C889(0.91); C1005(1.35); C315(1.09); C338(0.96)  LDD1615  [14]
 LDCM0412  CL24 HEK-293T C889(0.74); C1005(1.30); C315(1.06); C338(0.92)  LDD1616  [14]
 LDCM0413  CL25 HEK-293T C889(0.84); C1005(1.14); C315(0.93); C338(0.86)  LDD1617  [14]
 LDCM0414  CL26 HEK-293T C889(0.94); C1005(1.06); C315(1.01); C338(1.07)  LDD1618  [14]
 LDCM0415  CL27 HEK-293T C889(0.92); C1005(1.13); C315(0.94); C338(1.00)  LDD1619  [14]
 LDCM0416  CL28 HEK-293T C889(1.05); C1005(1.10); C315(1.14); C338(1.03)  LDD1620  [14]
 LDCM0417  CL29 HEK-293T C889(0.81); C1005(1.24); C315(0.91); C338(1.01)  LDD1621  [14]
 LDCM0418  CL3 HEK-293T C889(1.11); C1005(1.03); C315(0.96); C338(0.96)  LDD1622  [14]
 LDCM0419  CL30 HEK-293T C889(1.02); C1005(1.16); C315(0.90); C338(0.96)  LDD1623  [14]
 LDCM0420  CL31 HEK-293T C889(0.96); C1005(1.30); C315(1.09); C338(1.16)  LDD1624  [14]
 LDCM0421  CL32 HEK-293T C889(1.13); C1005(1.28); C315(0.94); C338(0.97)  LDD1625  [14]
 LDCM0422  CL33 HEK-293T C889(0.85); C1005(1.45); C315(0.82); C338(0.93)  LDD1626  [14]
 LDCM0423  CL34 HEK-293T C889(1.14); C1005(1.40); C315(0.86); C338(0.98)  LDD1627  [14]
 LDCM0424  CL35 HEK-293T C889(0.95); C1005(1.36); C315(0.99); C338(0.98)  LDD1628  [14]
 LDCM0425  CL36 HEK-293T C889(0.74); C1005(1.23); C315(1.14); C338(0.94)  LDD1629  [14]
 LDCM0426  CL37 HEK-293T C889(0.90); C1005(1.12); C315(1.14); C338(0.97)  LDD1630  [14]
 LDCM0428  CL39 HEK-293T C889(1.14); C1005(1.13); C315(1.07); C338(1.03)  LDD1632  [14]
 LDCM0429  CL4 HEK-293T C889(0.96); C1005(1.04); C315(1.04); C338(1.00)  LDD1633  [14]
 LDCM0430  CL40 HEK-293T C889(1.10); C1005(1.02); C315(1.12); C338(0.96)  LDD1634  [14]
 LDCM0431  CL41 HEK-293T C889(0.71); C1005(1.31); C315(0.91); C338(0.91)  LDD1635  [14]
 LDCM0432  CL42 HEK-293T C889(1.05); C1005(1.18); C315(0.92); C338(1.10)  LDD1636  [14]
 LDCM0433  CL43 HEK-293T C889(1.08); C1005(1.15); C315(1.03); C338(1.14)  LDD1637  [14]
 LDCM0434  CL44 HEK-293T C889(1.17); C1005(1.19); C315(1.04); C338(1.04)  LDD1638  [14]
 LDCM0435  CL45 HEK-293T C889(0.93); C1005(1.22); C315(0.96); C338(1.00)  LDD1639  [14]
 LDCM0436  CL46 HEK-293T C889(1.21); C1005(1.25); C315(1.00); C338(1.07)  LDD1640  [14]
 LDCM0437  CL47 HEK-293T C889(0.93); C1005(1.35); C315(1.05); C338(0.98)  LDD1641  [14]
 LDCM0438  CL48 HEK-293T C889(0.69); C1005(1.35); C315(0.87); C338(1.06)  LDD1642  [14]
 LDCM0439  CL49 HEK-293T C889(0.94); C1005(1.13); C315(0.84); C338(0.89)  LDD1643  [14]
 LDCM0440  CL5 HEK-293T C889(1.04); C1005(1.06); C315(0.95); C338(1.09)  LDD1644  [14]
 LDCM0441  CL50 HEK-293T C889(0.85); C1005(1.13); C315(1.04); C338(0.99)  LDD1645  [14]
 LDCM0443  CL52 HEK-293T C889(0.96); C1005(1.17); C315(1.01); C338(1.06)  LDD1646  [14]
 LDCM0444  CL53 HEK-293T C889(0.90); C1005(1.21); C315(0.92); C338(1.07)  LDD1647  [14]
 LDCM0445  CL54 HEK-293T C889(0.89); C1005(1.40); C315(0.93); C338(0.95)  LDD1648  [14]
 LDCM0446  CL55 HEK-293T C889(1.02); C1005(1.14); C315(1.02); C338(1.07)  LDD1649  [14]
 LDCM0447  CL56 HEK-293T C889(1.05); C1005(1.20); C315(0.93); C338(1.09)  LDD1650  [14]
 LDCM0448  CL57 HEK-293T C889(1.00); C1005(1.36); C315(0.98); C338(1.14)  LDD1651  [14]
 LDCM0449  CL58 HEK-293T C889(1.02); C1005(1.36); C315(0.84); C338(1.05)  LDD1652  [14]
 LDCM0450  CL59 HEK-293T C889(0.92); C1005(1.54); C315(1.08); C338(0.99)  LDD1653  [14]
 LDCM0451  CL6 HEK-293T C889(1.01); C1005(1.26); C315(0.89); C338(0.96)  LDD1654  [14]
 LDCM0452  CL60 HEK-293T C889(0.75); C1005(1.30); C315(1.28); C338(1.02)  LDD1655  [14]
 LDCM0453  CL61 HEK-293T C889(0.97); C1005(1.10); C315(1.04); C338(0.92)  LDD1656  [14]
 LDCM0454  CL62 HEK-293T C889(0.90); C1005(1.17); C315(1.03); C338(1.14)  LDD1657  [14]
 LDCM0455  CL63 HEK-293T C889(1.10); C1005(1.09); C315(1.08); C338(1.01)  LDD1658  [14]
 LDCM0456  CL64 HEK-293T C889(0.80); C1005(1.11); C315(1.11); C338(0.99)  LDD1659  [14]
 LDCM0457  CL65 HEK-293T C889(1.08); C1005(1.04); C315(0.90); C338(1.09)  LDD1660  [14]
 LDCM0458  CL66 HEK-293T C889(1.03); C1005(1.18); C315(1.06); C338(0.97)  LDD1661  [14]
 LDCM0459  CL67 HEK-293T C889(0.94); C1005(1.10); C315(1.00); C338(1.14)  LDD1662  [14]
 LDCM0460  CL68 HEK-293T C889(1.16); C1005(1.22); C315(0.95); C338(0.98)  LDD1663  [14]
 LDCM0461  CL69 HEK-293T C889(1.00); C1005(1.44); C315(0.98); C338(0.99)  LDD1664  [14]
 LDCM0462  CL7 HEK-293T C889(1.05); C1005(1.03); C315(1.16); C338(1.12)  LDD1665  [14]
 LDCM0463  CL70 HEK-293T C889(1.14); C1005(1.16); C315(0.99); C338(1.07)  LDD1666  [14]
 LDCM0464  CL71 HEK-293T C889(0.96); C1005(1.40); C315(1.03); C338(0.99)  LDD1667  [14]
 LDCM0465  CL72 HEK-293T C889(0.83); C1005(1.44); C315(1.22); C338(0.98)  LDD1668  [14]
 LDCM0466  CL73 HEK-293T C889(0.90); C1005(1.04); C315(0.79); C338(0.91)  LDD1669  [14]
 LDCM0467  CL74 HEK-293T C889(0.96); C1005(1.07); C315(1.10); C338(0.99)  LDD1670  [14]
 LDCM0469  CL76 HEK-293T C889(0.91); C1005(1.07); C315(1.11); C338(1.01)  LDD1672  [14]
 LDCM0470  CL77 HEK-293T C889(0.75); C1005(1.16); C315(0.81); C338(1.03)  LDD1673  [14]
 LDCM0471  CL78 HEK-293T C889(1.04); C1005(1.13); C315(1.01); C338(1.04)  LDD1674  [14]
 LDCM0472  CL79 HEK-293T C889(1.01); C1005(1.22); C315(1.05); C338(1.08)  LDD1675  [14]
 LDCM0473  CL8 HEK-293T C889(0.76); C1005(1.24); C315(0.92); C338(1.00)  LDD1676  [14]
 LDCM0474  CL80 HEK-293T C889(1.37); C1005(1.15); C315(0.94); C338(1.04)  LDD1677  [14]
 LDCM0475  CL81 HEK-293T C889(0.99); C1005(1.27); C315(0.95); C338(0.95)  LDD1678  [14]
 LDCM0476  CL82 HEK-293T C889(1.10); C1005(1.14); C315(0.93); C338(1.10)  LDD1679  [14]
 LDCM0477  CL83 HEK-293T C889(0.94); C1005(1.27); C315(1.03); C338(1.01)  LDD1680  [14]
 LDCM0478  CL84 HEK-293T C889(0.75); C1005(1.39); C315(1.04); C338(0.99)  LDD1681  [14]
 LDCM0479  CL85 HEK-293T C889(0.93); C1005(1.03); C315(0.82); C338(0.90)  LDD1682  [14]
 LDCM0480  CL86 HEK-293T C889(0.96); C1005(1.02); C315(0.91); C338(1.04)  LDD1683  [14]
 LDCM0481  CL87 HEK-293T C889(0.99); C1005(0.97); C315(1.06); C338(1.02)  LDD1684  [14]
 LDCM0482  CL88 HEK-293T C889(0.94); C1005(1.12); C315(0.97); C338(1.09)  LDD1685  [14]
 LDCM0483  CL89 HEK-293T C889(0.83); C1005(1.16); C315(0.92); C338(1.02)  LDD1686  [14]
 LDCM0484  CL9 HEK-293T C889(0.99); C1005(1.28); C315(1.04); C338(0.93)  LDD1687  [14]
 LDCM0485  CL90 HEK-293T C889(0.88); C1005(1.05); C315(0.85); C338(0.82)  LDD1688  [14]
 LDCM0486  CL91 HEK-293T C889(0.95); C1005(1.19); C315(1.06); C338(1.07)  LDD1689  [14]
 LDCM0487  CL92 HEK-293T C889(1.11); C1005(1.14); C315(0.97); C338(1.04)  LDD1690  [14]
 LDCM0488  CL93 HEK-293T C889(0.94); C1005(1.18); C315(0.90); C338(0.99)  LDD1691  [14]
 LDCM0489  CL94 HEK-293T C889(1.07); C1005(1.19); C315(1.01); C338(1.09)  LDD1692  [14]
 LDCM0490  CL95 HEK-293T C889(0.97); C1005(1.22); C315(0.95); C338(1.05)  LDD1693  [14]
 LDCM0491  CL96 HEK-293T C889(0.87); C1005(1.19); C315(1.37); C338(0.96)  LDD1694  [14]
 LDCM0492  CL97 HEK-293T C889(0.95); C1005(0.93); C315(0.84); C338(0.90)  LDD1695  [14]
 LDCM0493  CL98 HEK-293T C889(0.85); C1005(0.87); C315(0.99); C338(1.11)  LDD1696  [14]
 LDCM0494  CL99 HEK-293T C889(0.98); C1005(0.95); C315(0.99); C338(1.05)  LDD1697  [14]
 LDCM0495  E2913 HEK-293T C889(1.28); C1005(1.15); C315(1.02); C338(1.06)  LDD1698  [14]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C1002(3.57)  LDD1702  [3]
 LDCM0468  Fragment33 HEK-293T C889(0.99); C1005(1.09); C315(0.93); C338(0.94)  LDD1671  [14]
 LDCM0427  Fragment51 HEK-293T C889(1.02); C1005(1.11); C315(1.10); C338(0.93)  LDD1631  [14]
 LDCM0022  KB02 HEK-293T C398(1.16); C889(1.03)  LDD1492  [14]
 LDCM0023  KB03 HEK-293T C398(1.19); C889(1.03)  LDD1497  [14]
 LDCM0024  KB05 G361 C1005(2.23)  LDD3311  [15]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase SIAH1 (SIAH1) SINA (Seven in absentia) family Q8IUQ4
TNF receptor-associated factor 2 (TRAF2) TNF receptor-associated factor family Q12933
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
AP-2 complex subunit beta (AP2B1) Adaptor complexes large subunit family P63010
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cyclin-T1 (CCNT1) Cyclin family O60563
Golgin subfamily A member 2 (GOLGA2) GOLGA2 family Q08379
Microtubule-associated tumor suppressor candidate 2 (MTUS2) MTUS1 family Q5JR59
Protein AF-9 (MLLT3) . P42568
Protein ENL (MLLT1) . Q03111

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
11 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
12 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
13 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
14 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
15 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840