Details of the Target

General Information of Target

Target ID LDTP12630
Target Name Muscleblind-like protein 3 (MBNL3)
Gene Name MBNL3
Gene ID 55796
Synonyms
CHCR; MBLX39; MBXL; Muscleblind-like protein 3; Cys3His CCG1-required protein; Muscleblind-like X-linked protein; Protein HCHCR
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSENLDKSNVNEAGKSKSNDSEEGLEDAVEGADEALQKAIKSDSSSPQRVQRPHSSPPRF
VTVEELLETARGVTNMALAHEIVVNGDFQIKPVELPENSLKKRVKEIVHKAFWDCLSVQL
SEDPPAYDHAIKLVGEIKETLLSFLLPGHTRLRNQITEVLDLDLIKQEAENGALDISKLA
EFIIGMMGTLCAPARDEEVKKLKDIKEIVPLFREIFSVLDLMKVDMANFAISSIRPHLMQ
QSVEYERKKFQEILERQPNSLDFVTQWLEEASEDLMTQKYKHALPVGGMAAGSGDMPRLS
PVAVQNYAYLKLLKWDHLQRPFPETVLMDQSRFHELQLQLEQLTILGAVLLVTFSMAAPG
ISSQADFAEKLKMIVKILLTDMHLPSFHLKDVLTTIGEKVCLEVSSCLSLCGSSPFTTDK
ETVLKGQIQAVASPDDPIRRIMESRILTFLETYLASGHQKPLPTVPGGLSPVQRELEEVA
IKFARLVNYNKMVFCPYYDAILSKILVRS
Target Bioclass
Other
Family
Muscleblind family
Subcellular location
Nucleus
Function
Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. May play a role in myotonic dystrophy pathophysiology (DM). Could inhibit terminal muscle differentiation, acting at approximately the time of myogenin induction.
Uniprot ID
Q9NUK0
Ensemble ID
ENST00000370839.7
HGNC ID
HGNC:20564

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HT115 SNV: p.K77N .
IPC298 SNV: p.C44G .
OVKATE SNV: p.C216S .
OVTOKO SNV: p.Q248E .
PF382 SNV: p.A326V DBIA    Probe Info 
SKOV3 SNV: p.R270T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IPM
 Probe Info  		C180(0.00); C44(0.00)  LDD0241  [1]
DBIA
 Probe Info  		C99(0.80)  LDD3310  [2]
HHS-482
 Probe Info  		Y69(1.17)  LDD0285  [3]
HHS-475
 Probe Info  		Y69(1.01)  LDD0264  [4]
HHS-465
 Probe Info  		Y69(4.93)  LDD2237  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [6]
IA-alkyne
 Probe Info  		C44(0.00); C20(0.00); C195(0.00)  LDD0162  [7]
W1
 Probe Info  		C180(0.00); C20(0.00); C54(0.00); C44(0.00)  LDD0236  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0369  CL100 HEK-293T C44(0.77)  LDD1573  [8]
 LDCM0373  CL104 HEK-293T C44(1.00)  LDD1577  [8]
 LDCM0377  CL108 HEK-293T C44(1.03)  LDD1581  [8]
 LDCM0382  CL112 HEK-293T C44(0.92)  LDD1586  [8]
 LDCM0386  CL116 HEK-293T C44(1.02)  LDD1590  [8]
 LDCM0391  CL120 HEK-293T C44(1.04)  LDD1595  [8]
 LDCM0395  CL124 HEK-293T C44(0.91)  LDD1599  [8]
 LDCM0399  CL128 HEK-293T C44(1.03)  LDD1603  [8]
 LDCM0403  CL16 HEK-293T C44(1.14)  LDD1607  [8]
 LDCM0416  CL28 HEK-293T C44(1.01)  LDD1620  [8]
 LDCM0429  CL4 HEK-293T C44(0.96)  LDD1633  [8]
 LDCM0430  CL40 HEK-293T C44(0.96)  LDD1634  [8]
 LDCM0443  CL52 HEK-293T C44(0.97)  LDD1646  [8]
 LDCM0456  CL64 HEK-293T C44(0.97)  LDD1659  [8]
 LDCM0469  CL76 HEK-293T C44(0.94)  LDD1672  [8]
 LDCM0482  CL88 HEK-293T C44(0.85)  LDD1685  [8]
 LDCM0116  HHS-0101 DM93 Y69(1.01)  LDD0264  [4]
 LDCM0117  HHS-0201 DM93 Y69(0.78)  LDD0265  [4]
 LDCM0118  HHS-0301 DM93 Y69(0.72)  LDD0266  [4]
 LDCM0119  HHS-0401 DM93 Y69(0.53)  LDD0267  [4]
 LDCM0120  HHS-0701 DM93 Y69(1.64)  LDD0268  [4]
 LDCM0123  JWB131 DM93 Y69(1.17)  LDD0285  [3]
 LDCM0124  JWB142 DM93 Y69(0.61)  LDD0286  [3]
 LDCM0125  JWB146 DM93 Y69(1.43)  LDD0287  [3]
 LDCM0126  JWB150 DM93 Y69(2.93)  LDD0288  [3]
 LDCM0127  JWB152 DM93 Y69(1.72)  LDD0289  [3]
 LDCM0128  JWB198 DM93 Y69(1.05)  LDD0290  [3]
 LDCM0130  JWB211 DM93 Y69(1.74)  LDD0292  [3]
 LDCM0022  KB02 22RV1 C99(1.21)  LDD2243  [2]
 LDCM0023  KB03 22RV1 C99(1.64)  LDD2660  [2]
 LDCM0024  KB05 COLO792 C99(0.80)  LDD3310  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proline-rich protein 20D (PRR20D) PRR20 family P86480
Disabled homolog 1 (DAB1) . O75553

References

1 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Chemoproteomic profiling of kinases in live cells using electrophilic sulfonyl triazole probes. Chem Sci. 2021 Jan 21;12(9):3295-3307. doi: 10.1039/d0sc06623k.
4 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
5 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402