Details of the Target

General Information of Target

Target ID LDTP12350
Target Name H/ACA ribonucleoprotein complex subunit 3 (NOP10)
Gene Name NOP10
Gene ID 55505
Synonyms
NOLA3; H/ACA ribonucleoprotein complex subunit 3; Nucleolar protein 10; Nucleolar protein family A member 3; snoRNP protein NOP10
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAVTLSLLLGGRVCAAVTRCGFATRGVAGPGPIGREPDPDSDWEPEERELQEVESTLKRQ
KQAIRFQKIRRQMEAPGAPPRTLTWEAMEQIRYLHEEFPESWSVPRLAEGFDVSTDVIRR
VLKSKFLPTLEQKLKQDQKVLKKAGLAHSLQHLRGSGNTSKLLPAGHSVSGSLLMPGHEA
SSKDPNHSTALKVIESDTHRTNTPRRRKGRNKEIQDLEESFVPVAAPLGHPRELQKYSSD
SESPRGTGSGALPSGQKLEELKAEEPDNFSSKVVQRGREFFDSNGNFLYRI
Target Bioclass
Other
Family
NOP10 family
Subcellular location
Nucleus, nucleolus
Function
Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
Uniprot ID
Q9NPE3
Ensemble ID
ENST00000328848.6
HGNC ID
HGNC:14378

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C28(2.85)  LDD3311  [1]
BTD
 Probe Info  		C28(1.20)  LDD2090  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0036  [3]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [3]
IPM
 Probe Info  		N.A.  LDD0025  [4]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [4]
TFBX
 Probe Info  		N.A.  LDD0027  [4]
VSF
 Probe Info  		N.A.  LDD0007  [5]
Acrolein
 Probe Info  		N.A.  LDD0217  [6]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [6]
Methacrolein
 Probe Info  		N.A.  LDD0218  [6]
W1
 Probe Info  		N.A.  LDD0236  [7]
NAIA_5
 Probe Info  		N.A.  LDD2223  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C28(0.59)  LDD2142  [2]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C28(0.90)  LDD2112  [2]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C28(0.66)  LDD2117  [2]
 LDCM0510  3-(4-(Hydroxydiphenylmethyl)piperidin-1-yl)-3-oxopropanenitrile MDA-MB-231 C28(0.90)  LDD2103  [2]
 LDCM0281  AC21 HEK-293T C28(1.12)  LDD1520  [9]
 LDCM0284  AC24 HEK-293T C28(1.01)  LDD1523  [9]
 LDCM0289  AC29 HEK-293T C28(1.12)  LDD1528  [9]
 LDCM0293  AC32 HEK-293T C28(1.12)  LDD1532  [9]
 LDCM0298  AC37 HEK-293T C28(1.17)  LDD1537  [9]
 LDCM0302  AC40 HEK-293T C28(1.10)  LDD1541  [9]
 LDCM0307  AC45 HEK-293T C28(1.15)  LDD1546  [9]
 LDCM0310  AC48 HEK-293T C28(1.09)  LDD1549  [9]
 LDCM0312  AC5 HEK-293T C28(1.12)  LDD1551  [9]
 LDCM0316  AC53 HEK-293T C28(1.08)  LDD1555  [9]
 LDCM0319  AC56 HEK-293T C28(1.06)  LDD1558  [9]
 LDCM0325  AC61 HEK-293T C28(1.04)  LDD1564  [9]
 LDCM0328  AC64 HEK-293T C28(0.97)  LDD1567  [9]
 LDCM0345  AC8 HEK-293T C28(1.04)  LDD1569  [9]
 LDCM0520  AKOS000195272 MDA-MB-231 C28(0.48)  LDD2113  [2]
 LDCM0248  AKOS034007472 HEK-293T C28(1.10)  LDD1511  [9]
 LDCM0275  AKOS034007705 HEK-293T C28(0.97)  LDD1514  [9]
 LDCM0498  BS-3668 MDA-MB-231 C28(0.45)  LDD2091  [2]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [6]
 LDCM0371  CL102 HEK-293T C28(0.90)  LDD1575  [9]
 LDCM0375  CL106 HEK-293T C28(0.85)  LDD1579  [9]
 LDCM0380  CL110 HEK-293T C28(0.86)  LDD1584  [9]
 LDCM0384  CL114 HEK-293T C28(0.90)  LDD1588  [9]
 LDCM0388  CL118 HEK-293T C28(0.91)  LDD1592  [9]
 LDCM0390  CL12 HEK-293T C28(1.07)  LDD1594  [9]
 LDCM0393  CL122 HEK-293T C28(0.87)  LDD1597  [9]
 LDCM0397  CL126 HEK-293T C28(0.73)  LDD1601  [9]
 LDCM0401  CL14 HEK-293T C28(0.98)  LDD1605  [9]
 LDCM0407  CL2 HEK-293T C28(0.98)  LDD1611  [9]
 LDCM0409  CL21 HEK-293T C28(1.53)  LDD1613  [9]
 LDCM0412  CL24 HEK-293T C28(0.82)  LDD1616  [9]
 LDCM0414  CL26 HEK-293T C28(0.92)  LDD1618  [9]
 LDCM0422  CL33 HEK-293T C28(1.40)  LDD1626  [9]
 LDCM0425  CL36 HEK-293T C28(0.80)  LDD1629  [9]
 LDCM0435  CL45 HEK-293T C28(1.35)  LDD1639  [9]
 LDCM0438  CL48 HEK-293T C28(0.88)  LDD1642  [9]
 LDCM0441  CL50 HEK-293T C28(0.86)  LDD1645  [9]
 LDCM0448  CL57 HEK-293T C28(1.37)  LDD1651  [9]
 LDCM0452  CL60 HEK-293T C28(0.90)  LDD1655  [9]
 LDCM0454  CL62 HEK-293T C28(0.91)  LDD1657  [9]
 LDCM0461  CL69 HEK-293T C28(1.34)  LDD1664  [9]
 LDCM0465  CL72 HEK-293T C28(0.96)  LDD1668  [9]
 LDCM0467  CL74 HEK-293T C28(0.85)  LDD1670  [9]
 LDCM0475  CL81 HEK-293T C28(1.15)  LDD1678  [9]
 LDCM0478  CL84 HEK-293T C28(0.96)  LDD1681  [9]
 LDCM0480  CL86 HEK-293T C28(0.80)  LDD1683  [9]
 LDCM0484  CL9 HEK-293T C28(1.37)  LDD1687  [9]
 LDCM0488  CL93 HEK-293T C28(1.14)  LDD1691  [9]
 LDCM0491  CL96 HEK-293T C28(0.89)  LDD1694  [9]
 LDCM0493  CL98 HEK-293T C28(1.00)  LDD1696  [9]
 LDCM0625  F8 Ramos C28(1.22)  LDD2187  [10]
 LDCM0572  Fragment10 Ramos C28(0.55)  LDD2189  [10]
 LDCM0573  Fragment11 Ramos C28(2.14)  LDD2190  [10]
 LDCM0574  Fragment12 Ramos C28(0.37)  LDD2191  [10]
 LDCM0575  Fragment13 Ramos C28(0.71)  LDD2192  [10]
 LDCM0576  Fragment14 Ramos C28(1.08)  LDD2193  [10]
 LDCM0579  Fragment20 Ramos C28(0.46)  LDD2194  [10]
 LDCM0580  Fragment21 Ramos C28(0.69)  LDD2195  [10]
 LDCM0582  Fragment23 Ramos C28(1.00)  LDD2196  [10]
 LDCM0578  Fragment27 Ramos C28(0.86)  LDD2197  [10]
 LDCM0586  Fragment28 Ramos C28(0.74)  LDD2198  [10]
 LDCM0588  Fragment30 Ramos C28(0.74)  LDD2199  [10]
 LDCM0589  Fragment31 Ramos C28(0.89)  LDD2200  [10]
 LDCM0590  Fragment32 Ramos C28(0.61)  LDD2201  [10]
 LDCM0468  Fragment33 Ramos C28(0.87)  LDD2202  [10]
 LDCM0596  Fragment38 Ramos C28(0.97)  LDD2203  [10]
 LDCM0566  Fragment4 Ramos C28(0.68)  LDD2184  [10]
 LDCM0427  Fragment51 HEK-293T C28(0.85)  LDD1631  [9]
 LDCM0610  Fragment52 Ramos C28(0.30)  LDD2204  [10]
 LDCM0614  Fragment56 Ramos C28(0.57)  LDD2205  [10]
 LDCM0569  Fragment7 Ramos C28(0.60)  LDD2186  [10]
 LDCM0571  Fragment9 Ramos C28(0.31)  LDD2188  [10]
 LDCM0107  IAA HeLa N.A.  LDD0221  [6]
 LDCM0022  KB02 Ramos C28(0.55)  LDD2182  [10]
 LDCM0023  KB03 MDA-MB-231 C28(0.90)  LDD1701  [2]
 LDCM0024  KB05 G361 C28(2.85)  LDD3311  [1]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C28(0.96)  LDD2102  [2]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C28(1.20)  LDD2090  [2]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C28(0.98)  LDD2092  [2]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C28(1.34)  LDD2094  [2]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C28(1.12)  LDD2096  [2]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C28(1.04)  LDD2098  [2]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C28(0.83)  LDD2100  [2]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C28(0.63)  LDD2101  [2]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C28(0.59)  LDD2104  [2]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C28(0.68)  LDD2106  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C28(0.91)  LDD2107  [2]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C28(0.78)  LDD2108  [2]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C28(0.60)  LDD2110  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C28(0.80)  LDD2111  [2]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C28(0.57)  LDD2114  [2]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C28(0.48)  LDD2115  [2]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C28(0.74)  LDD2116  [2]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C28(1.38)  LDD2118  [2]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C28(0.93)  LDD2120  [2]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C28(1.27)  LDD2122  [2]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C28(1.52)  LDD2124  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C28(1.50)  LDD2125  [2]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C28(1.03)  LDD2126  [2]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C28(1.05)  LDD2128  [2]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C28(0.60)  LDD2129  [2]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C28(0.49)  LDD2133  [2]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C28(0.42)  LDD2134  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C28(0.68)  LDD2136  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C28(1.18)  LDD2137  [2]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C28(0.72)  LDD2141  [2]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C28(1.08)  LDD2143  [2]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C28(0.91)  LDD2145  [2]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C28(0.39)  LDD2148  [2]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C28(1.35)  LDD2151  [2]
 LDCM0559  Nucleophilic fragment 9b MDA-MB-231 C28(2.53)  LDD2153  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
H/ACA ribonucleoprotein complex subunit 2 (NHP2) Eukaryotic ribosomal protein eL8 family Q9NX24
Calcium-binding protein 2 (CABP2) . Q9NPB3

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
5 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
6 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
7 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
10 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578