Details of the Target

General Information of Target

Target ID LDTP11356
Target Name Adenosine 5'-monophosphoramidase HINT2 (HINT2)
Gene Name HINT2
Gene ID 84681
Synonyms
Adenosine 5'-monophosphoramidase HINT2; EC 3.9.1.-; HINT-3; HIT-17kDa; Histidine triad nucleotide-binding protein 2, mitochondrial; HINT-2; PKCI-1-related HIT protein
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MALRRPPRLRLCARLPDFFLLLLFRGCLIGAVNLKSSNRTPVVQEFESVELSCIITDSQT
SDPRIEWKKIQDEQTTYVFFDNKIQGDLAGRAEILGKTSLKIWNVTRRDSALYRCEVVAR
NDRKEIDEIVIELTVQVKPVTPVCRVPKAVPVGKMATLHCQESEGHPRPHYSWYRNDVPL
PTDSRANPRFRNSSFHLNSETGTLVFTAVHKDDSGQYYCIASNDAGSARCEEQEMEVYDL
NIGGIIGGVLVVLAVLALITLGICCAYRRGYFINNKQDGESYKNPGKPDGVNYIRTDEEG
DFRHKSSFVI
Target Bioclass
Enzyme
Family
HINT family
Subcellular location
Mitochondrion
Function
Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2. Hydrolyzes adenosine 5'-O-p-nitrophenylphosphoramidate (AMP-pNA). Hydrolyzes fluorogenic purine nucleoside tryptamine phosphoramidates in vitro. May be involved in steroid biosynthesis. May play a role in apoptosis.
Uniprot ID
Q9BX68
Ensemble ID
ENST00000259667.6
HGNC ID
HGNC:18344

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C-Sul
 Probe Info  		2.34  LDD0066  [1]
STPyne
 Probe Info  		K119(7.14); K123(10.00)  LDD0277  [2]
DBIA
 Probe Info  		C75(1.60)  LDD3312  [3]
Acrolein
 Probe Info  		H113(0.00); H88(0.00)  LDD0227  [4]
m-APA
 Probe Info  		N.A.  LDD2231  [5]
1d-yne
 Probe Info  		N.A.  LDD0358  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0175  [7]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [8]
W1
 Probe Info  		D107(0.00); E104(0.00); E105(0.00); E106(0.00)  LDD0236  [9]
NAIA_5
 Probe Info  		N.A.  LDD2223  [10]
HHS-465
 Probe Info  		N.A.  LDD2240  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C75(0.93)  LDD1507  [12]
 LDCM0215  AC10 HEK-293T C75(1.03)  LDD1508  [12]
 LDCM0226  AC11 HEK-293T C75(1.03)  LDD1509  [12]
 LDCM0237  AC12 HEK-293T C75(1.22)  LDD1510  [12]
 LDCM0259  AC14 HEK-293T C75(0.86)  LDD1512  [12]
 LDCM0263  AC143 PaTu 8988t C75(1.48)  LDD1142  [13]
 LDCM0264  AC144 PaTu 8988t C75(1.69)  LDD1143  [13]
 LDCM0265  AC145 PaTu 8988t C75(1.00)  LDD1144  [13]
 LDCM0266  AC146 PaTu 8988t C75(0.93)  LDD1145  [13]
 LDCM0267  AC147 PaTu 8988t C75(3.51)  LDD1146  [13]
 LDCM0268  AC148 PaTu 8988t C75(1.55)  LDD1147  [13]
 LDCM0269  AC149 PaTu 8988t C75(1.25)  LDD1148  [13]
 LDCM0271  AC150 PaTu 8988t C75(1.44)  LDD1150  [13]
 LDCM0272  AC151 PaTu 8988t C75(0.99)  LDD1151  [13]
 LDCM0273  AC152 PaTu 8988t C75(1.37)  LDD1152  [13]
 LDCM0274  AC153 PaTu 8988t C75(1.48)  LDD1153  [13]
 LDCM0621  AC154 PaTu 8988t C75(1.53)  LDD2166  [13]
 LDCM0622  AC155 PaTu 8988t C75(1.12)  LDD2167  [13]
 LDCM0623  AC156 PaTu 8988t C75(1.13)  LDD2168  [13]
 LDCM0624  AC157 PaTu 8988t C75(1.43)  LDD2169  [13]
 LDCM0276  AC17 HEK-293T C75(1.08)  LDD1515  [12]
 LDCM0277  AC18 HEK-293T C75(1.01)  LDD1516  [12]
 LDCM0278  AC19 HEK-293T C75(0.94)  LDD1517  [12]
 LDCM0279  AC2 HEK-293T C75(1.02)  LDD1518  [12]
 LDCM0280  AC20 HEK-293T C75(1.04)  LDD1519  [12]
 LDCM0281  AC21 HEK-293T C75(1.03)  LDD1520  [12]
 LDCM0282  AC22 HEK-293T C75(0.98)  LDD1521  [12]
 LDCM0284  AC24 HEK-293T C75(0.96)  LDD1523  [12]
 LDCM0285  AC25 HEK-293T C75(1.01)  LDD1524  [12]
 LDCM0286  AC26 HEK-293T C75(1.05)  LDD1525  [12]
 LDCM0287  AC27 HEK-293T C75(1.01)  LDD1526  [12]
 LDCM0288  AC28 HEK-293T C75(1.09)  LDD1527  [12]
 LDCM0289  AC29 HEK-293T C75(0.99)  LDD1528  [12]
 LDCM0290  AC3 HEK-293T C75(0.94)  LDD1529  [12]
 LDCM0291  AC30 HEK-293T C75(1.04)  LDD1530  [12]
 LDCM0293  AC32 HEK-293T C75(1.05)  LDD1532  [12]
 LDCM0294  AC33 HEK-293T C75(1.01)  LDD1533  [12]
 LDCM0295  AC34 HEK-293T C75(1.19)  LDD1534  [12]
 LDCM0296  AC35 HEK-293T C75(1.12)  LDD1535  [12]
 LDCM0297  AC36 HEK-293T C75(1.06)  LDD1536  [12]
 LDCM0298  AC37 HEK-293T C75(1.07)  LDD1537  [12]
 LDCM0299  AC38 HEK-293T C75(0.91)  LDD1538  [12]
 LDCM0301  AC4 HEK-293T C75(1.01)  LDD1540  [12]
 LDCM0302  AC40 HEK-293T C75(1.08)  LDD1541  [12]
 LDCM0303  AC41 HEK-293T C75(1.11)  LDD1542  [12]
 LDCM0304  AC42 HEK-293T C75(0.98)  LDD1543  [12]
 LDCM0305  AC43 HEK-293T C75(1.29)  LDD1544  [12]
 LDCM0306  AC44 HEK-293T C75(1.08)  LDD1545  [12]
 LDCM0307  AC45 HEK-293T C75(1.08)  LDD1546  [12]
 LDCM0308  AC46 HEK-293T C75(1.05)  LDD1547  [12]
 LDCM0310  AC48 HEK-293T C75(1.11)  LDD1549  [12]
 LDCM0311  AC49 HEK-293T C75(1.11)  LDD1550  [12]
 LDCM0312  AC5 HEK-293T C75(0.92)  LDD1551  [12]
 LDCM0313  AC50 HEK-293T C75(1.03)  LDD1552  [12]
 LDCM0314  AC51 HEK-293T C75(1.06)  LDD1553  [12]
 LDCM0315  AC52 HEK-293T C75(1.15)  LDD1554  [12]
 LDCM0316  AC53 HEK-293T C75(1.01)  LDD1555  [12]
 LDCM0317  AC54 HEK-293T C75(0.93)  LDD1556  [12]
 LDCM0319  AC56 HEK-293T C75(1.03)  LDD1558  [12]
 LDCM0320  AC57 HEK-293T C75(1.04)  LDD1559  [12]
 LDCM0321  AC58 HEK-293T C75(1.06)  LDD1560  [12]
 LDCM0322  AC59 HEK-293T C75(1.01)  LDD1561  [12]
 LDCM0323  AC6 HEK-293T C75(0.89)  LDD1562  [12]
 LDCM0324  AC60 HEK-293T C75(1.04)  LDD1563  [12]
 LDCM0325  AC61 HEK-293T C75(1.16)  LDD1564  [12]
 LDCM0326  AC62 HEK-293T C75(0.95)  LDD1565  [12]
 LDCM0328  AC64 HEK-293T C75(1.09)  LDD1567  [12]
 LDCM0345  AC8 HEK-293T C75(0.91)  LDD1569  [12]
 LDCM0248  AKOS034007472 HEK-293T C75(1.07)  LDD1511  [12]
 LDCM0356  AKOS034007680 HEK-293T C75(1.11)  LDD1570  [12]
 LDCM0275  AKOS034007705 HEK-293T C75(1.01)  LDD1514  [12]
 LDCM0368  CL10 HEK-293T C75(0.96)  LDD1572  [12]
 LDCM0369  CL100 HEK-293T C75(1.00)  LDD1573  [12]
 LDCM0371  CL102 HEK-293T C75(1.08)  LDD1575  [12]
 LDCM0372  CL103 HEK-293T C75(0.96)  LDD1576  [12]
 LDCM0373  CL104 HEK-293T C75(0.89)  LDD1577  [12]
 LDCM0375  CL106 HEK-293T C75(1.01)  LDD1579  [12]
 LDCM0376  CL107 HEK-293T C75(0.99)  LDD1580  [12]
 LDCM0377  CL108 HEK-293T C75(0.79)  LDD1581  [12]
 LDCM0380  CL110 HEK-293T C75(1.14)  LDD1584  [12]
 LDCM0381  CL111 HEK-293T C75(1.00)  LDD1585  [12]
 LDCM0382  CL112 HEK-293T C75(0.98)  LDD1586  [12]
 LDCM0384  CL114 HEK-293T C75(1.11)  LDD1588  [12]
 LDCM0385  CL115 HEK-293T C75(0.98)  LDD1589  [12]
 LDCM0386  CL116 HEK-293T C75(1.23)  LDD1590  [12]
 LDCM0388  CL118 HEK-293T C75(1.04)  LDD1592  [12]
 LDCM0389  CL119 HEK-293T C75(0.99)  LDD1593  [12]
 LDCM0390  CL12 HEK-293T C75(1.11)  LDD1594  [12]
 LDCM0391  CL120 HEK-293T C75(1.12)  LDD1595  [12]
 LDCM0393  CL122 HEK-293T C75(1.14)  LDD1597  [12]
 LDCM0394  CL123 HEK-293T C75(0.97)  LDD1598  [12]
 LDCM0395  CL124 HEK-293T C75(0.99)  LDD1599  [12]
 LDCM0397  CL126 HEK-293T C75(1.01)  LDD1601  [12]
 LDCM0398  CL127 HEK-293T C75(0.98)  LDD1602  [12]
 LDCM0399  CL128 HEK-293T C75(0.81)  LDD1603  [12]
 LDCM0401  CL14 HEK-293T C75(1.09)  LDD1605  [12]
 LDCM0402  CL15 HEK-293T C75(0.95)  LDD1606  [12]
 LDCM0403  CL16 PaTu 8988t C75(0.87)  LDD1282  [13]
 LDCM0404  CL17 PaTu 8988t C75(0.70)  LDD1283  [13]
 LDCM0405  CL18 PaTu 8988t C75(0.64)  LDD1284  [13]
 LDCM0406  CL19 PaTu 8988t C75(0.87)  LDD1285  [13]
 LDCM0407  CL2 HEK-293T C75(1.14)  LDD1611  [12]
 LDCM0408  CL20 PaTu 8988t C75(0.68)  LDD1287  [13]
 LDCM0409  CL21 PaTu 8988t C75(0.43)  LDD1288  [13]
 LDCM0410  CL22 PaTu 8988t C75(0.72)  LDD1289  [13]
 LDCM0411  CL23 PaTu 8988t C75(0.47)  LDD1290  [13]
 LDCM0412  CL24 PaTu 8988t C75(0.78)  LDD1291  [13]
 LDCM0413  CL25 PaTu 8988t C75(0.64)  LDD1292  [13]
 LDCM0414  CL26 PaTu 8988t C75(0.71)  LDD1293  [13]
 LDCM0415  CL27 PaTu 8988t C75(1.18)  LDD1294  [13]
 LDCM0416  CL28 PaTu 8988t C75(0.61)  LDD1295  [13]
 LDCM0417  CL29 PaTu 8988t C75(0.45)  LDD1296  [13]
 LDCM0418  CL3 HEK-293T C75(1.04)  LDD1622  [12]
 LDCM0419  CL30 PaTu 8988t C75(0.45)  LDD1298  [13]
 LDCM0420  CL31 PaTu 8988t C75(0.76)  LDD1299  [13]
 LDCM0421  CL32 PaTu 8988t C75(0.76)  LDD1300  [13]
 LDCM0422  CL33 PaTu 8988t C75(0.64)  LDD1301  [13]
 LDCM0423  CL34 PaTu 8988t C75(0.60)  LDD1302  [13]
 LDCM0424  CL35 PaTu 8988t C75(0.67)  LDD1303  [13]
 LDCM0425  CL36 PaTu 8988t C75(0.41)  LDD1304  [13]
 LDCM0426  CL37 PaTu 8988t C75(0.54)  LDD1305  [13]
 LDCM0428  CL39 PaTu 8988t C75(0.67)  LDD1307  [13]
 LDCM0429  CL4 HEK-293T C75(0.95)  LDD1633  [12]
 LDCM0430  CL40 PaTu 8988t C75(0.98)  LDD1309  [13]
 LDCM0431  CL41 PaTu 8988t C75(0.59)  LDD1310  [13]
 LDCM0432  CL42 PaTu 8988t C75(0.87)  LDD1311  [13]
 LDCM0433  CL43 PaTu 8988t C75(0.69)  LDD1312  [13]
 LDCM0434  CL44 PaTu 8988t C75(0.71)  LDD1313  [13]
 LDCM0435  CL45 PaTu 8988t C75(0.62)  LDD1314  [13]
 LDCM0436  CL46 HEK-293T C75(1.12)  LDD1640  [12]
 LDCM0438  CL48 HEK-293T C75(0.94)  LDD1642  [12]
 LDCM0440  CL5 HEK-293T C75(1.11)  LDD1644  [12]
 LDCM0441  CL50 HEK-293T C75(1.19)  LDD1645  [12]
 LDCM0443  CL52 HEK-293T C75(1.01)  LDD1646  [12]
 LDCM0444  CL53 HEK-293T C75(1.07)  LDD1647  [12]
 LDCM0445  CL54 HEK-293T C75(0.95)  LDD1648  [12]
 LDCM0446  CL55 HEK-293T C75(1.04)  LDD1649  [12]
 LDCM0447  CL56 HEK-293T C75(1.08)  LDD1650  [12]
 LDCM0448  CL57 HEK-293T C75(0.85)  LDD1651  [12]
 LDCM0449  CL58 HEK-293T C75(0.76)  LDD1652  [12]
 LDCM0451  CL6 HEK-293T C75(1.16)  LDD1654  [12]
 LDCM0452  CL60 HEK-293T C75(1.01)  LDD1655  [12]
 LDCM0454  CL62 HEK-293T C75(1.04)  LDD1657  [12]
 LDCM0455  CL63 HEK-293T C75(1.00)  LDD1658  [12]
 LDCM0456  CL64 HEK-293T C75(0.87)  LDD1659  [12]
 LDCM0457  CL65 HEK-293T C75(1.08)  LDD1660  [12]
 LDCM0458  CL66 HEK-293T C75(0.25)  LDD1661  [12]
 LDCM0459  CL67 HEK-293T C75(1.08)  LDD1662  [12]
 LDCM0460  CL68 HEK-293T C75(1.05)  LDD1663  [12]
 LDCM0461  CL69 HEK-293T C75(0.89)  LDD1664  [12]
 LDCM0462  CL7 HEK-293T C75(1.14)  LDD1665  [12]
 LDCM0463  CL70 HEK-293T C75(1.08)  LDD1666  [12]
 LDCM0465  CL72 HEK-293T C75(0.94)  LDD1668  [12]
 LDCM0467  CL74 HEK-293T C75(0.99)  LDD1670  [12]
 LDCM0469  CL76 PaTu 8988t C75(0.64)  LDD1348  [13]
 LDCM0470  CL77 PaTu 8988t C75(1.04)  LDD1349  [13]
 LDCM0471  CL78 PaTu 8988t C75(0.95)  LDD1350  [13]
 LDCM0472  CL79 PaTu 8988t C75(0.75)  LDD1351  [13]
 LDCM0473  CL8 HEK-293T C75(1.00)  LDD1676  [12]
 LDCM0474  CL80 PaTu 8988t C75(0.71)  LDD1353  [13]
 LDCM0475  CL81 PaTu 8988t C75(0.84)  LDD1354  [13]
 LDCM0476  CL82 PaTu 8988t C75(0.78)  LDD1355  [13]
 LDCM0477  CL83 PaTu 8988t C75(0.94)  LDD1356  [13]
 LDCM0478  CL84 PaTu 8988t C75(0.77)  LDD1357  [13]
 LDCM0479  CL85 PaTu 8988t C75(1.24)  LDD1358  [13]
 LDCM0480  CL86 PaTu 8988t C75(0.59)  LDD1359  [13]
 LDCM0481  CL87 PaTu 8988t C75(0.82)  LDD1360  [13]
 LDCM0482  CL88 PaTu 8988t C75(0.79)  LDD1361  [13]
 LDCM0483  CL89 PaTu 8988t C75(0.77)  LDD1362  [13]
 LDCM0484  CL9 HEK-293T C75(0.51)  LDD1687  [12]
 LDCM0485  CL90 PaTu 8988t C75(0.91)  LDD1364  [13]
 LDCM0486  CL91 HEK-293T C75(0.98)  LDD1689  [12]
 LDCM0487  CL92 HEK-293T C75(1.12)  LDD1690  [12]
 LDCM0488  CL93 HEK-293T C75(0.93)  LDD1691  [12]
 LDCM0489  CL94 HEK-293T C75(1.01)  LDD1692  [12]
 LDCM0491  CL96 HEK-293T C75(1.02)  LDD1694  [12]
 LDCM0493  CL98 HEK-293T C75(1.05)  LDD1696  [12]
 LDCM0494  CL99 HEK-293T C75(0.86)  LDD1697  [12]
 LDCM0495  E2913 HEK-293T C75(0.89)  LDD1698  [12]
 LDCM0468  Fragment33 HEK-293T C75(1.06)  LDD1671  [12]
 LDCM0427  Fragment51 PaTu 8988t C75(0.38)  LDD1306  [13]
 LDCM0022  KB02 22RV1 C75(1.44)  LDD2243  [3]
 LDCM0023  KB03 22RV1 C75(1.89)  LDD2660  [3]
 LDCM0024  KB05 HMCB C75(1.60)  LDD3312  [3]
 LDCM0109  NEM HeLa H113(0.00); H88(0.00)  LDD0227  [4]

References

1 Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. Anal Chem. 2022 Mar 15;94(10):4366-4372. doi: 10.1021/acs.analchem.1c05129. Epub 2022 Mar 4.
2 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
5 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
6 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
9 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
10 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
11 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
13 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.