Details of the Target

General Information of Target

Target ID LDTP10348
Target Name E3 ubiquitin-protein ligase RNF185 (RNF185)
Gene Name RNF185
Gene ID 91445
Synonyms
E3 ubiquitin-protein ligase RNF185; EC 2.3.2.27; RING finger protein 185
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAGSDAEWVTIANNLLFKCHIHLRIHELQDCDANVFIALYQSILGEKVPDLIVIPRSQED
DAHNVQAVIDSLALDYLQVSLSHITGENIVKGDKESIKNLLEIFDGLLEYLTERISETSH
EKSETEQYFKESDRGERLEEPESTKESKSSWKRVSFGRCSLSSEMLGPSWDGDEAESTGE
IIRLGDTAHTFSLRSNGAQCPNEMLSKKALASPSSKSHEDMLYPPSVLSKSRTSFVEDTE
TLSVSGIPNARKLGEPIRAAIPLHPPYHPSEPRAPCPIGKEYLHSSHCSPAVNSTGEHTE
FSGDLDDGLFLISKLPKGSKWEVYPAQVQGPRTRKPPKGKRNENRATASSCNSPFPQRPR
KRLTEQELHDVSEKLSQRLSELDWMLKSALGDRIKEKTDHKEENTGNEEVEDGTEETLSQ
HSDGIVEYGPKKSRPGLSMRRKPPYRSHSLSPSPVNKHKQFHLERKRQRKPRETDVRQFQ
AQAFTEAFERELRRHKVQENIGPLRIHEKEEETEKIYRGEAVRKGTPECSQPWKIYSRKT
TTQSLRGGLPKPNKAVPMKVSEHSLLPLMLEQFPFLYVSGPTLSKMWKQQIAQVEQLKKE
ACRENRSKKKLQDEIEEALRRHDLLTTLVKKEYEHNKRLQDFKDCIRRQRLTQSKIKENR
QQIVRARKYYDDYRVQLCAKMMRMRTREEMIFKKLFEEGLNIQKQRLRDLRNYAKEKRDE
QRRRHQDELDSMENYYKDQFSLLAEAISQEHQELKAREKSQAQTLHKVKRELRSKMEKEI
QQLQDMITQNDDDVFFRELEAERFRSRLQLASFQYSKSPSL
Target Bioclass
Enzyme
Subcellular location
Mitochondrion outer membrane
Function
E3 ubiquitin-protein ligase that regulates selective mitochondrial autophagy by mediating 'Lys-63'-linked polyubiquitination of BNIP1. Acts in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway, which targets misfolded proteins that accumulate in the endoplasmic reticulum (ER) for ubiquitination and subsequent proteasome-mediated degradation. Protects cells from ER stress-induced apoptosis. Responsible for the cotranslational ubiquitination and degradation of CFTR in the ERAD pathway. Also acts as a regulator of the innate antiviral response by catalyzing 'Lys-27'-linked polyubiquitination of CGAS at 'Lys-173' and 'Lys-384', thereby promoting CGAS cyclic GMP-AMP synthase activity. Preferentially associates with the E2 enzymes UBE2J1 and UBE2J2.
Uniprot ID
Q96GF1
Ensemble ID
ENST00000266252.8
HGNC ID
HGNC:26783

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
KYSE510 SNV: p.A27P .
NCIH1155 SNV: p.P160L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K80(1.30)  LDD0277  [1]
DBIA
 Probe Info  		C76(1.63); C79(1.63)  LDD0080  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C76(1.13); C79(1.13)  LDD0532  [2]
 LDCM0226  AC11 HCT 116 C76(1.27); C79(1.27)  LDD0543  [2]
 LDCM0230  AC113 HCT 116 C76(0.99); C79(0.99)  LDD0547  [2]
 LDCM0231  AC114 HCT 116 C76(1.06); C79(0.82)  LDD0548  [2]
 LDCM0232  AC115 HCT 116 C76(1.06); C79(1.01)  LDD0549  [2]
 LDCM0233  AC116 HCT 116 C76(1.02); C79(0.82)  LDD0550  [2]
 LDCM0234  AC117 HCT 116 C76(0.96); C79(0.97)  LDD0551  [2]
 LDCM0235  AC118 HCT 116 C76(0.90); C79(0.87)  LDD0552  [2]
 LDCM0236  AC119 HCT 116 C76(0.88); C79(0.89)  LDD0553  [2]
 LDCM0237  AC12 HCT 116 C76(1.36); C79(1.36)  LDD0554  [2]
 LDCM0238  AC120 HCT 116 C76(0.95); C79(0.96)  LDD0555  [2]
 LDCM0239  AC121 HCT 116 C76(0.79); C79(1.17)  LDD0556  [2]
 LDCM0240  AC122 HCT 116 C76(0.81); C79(0.94)  LDD0557  [2]
 LDCM0241  AC123 HCT 116 C76(0.78); C79(0.81)  LDD0558  [2]
 LDCM0242  AC124 HCT 116 C76(0.83); C79(0.93)  LDD0559  [2]
 LDCM0243  AC125 HCT 116 C76(0.78); C79(0.98)  LDD0560  [2]
 LDCM0244  AC126 HCT 116 C76(0.78); C79(0.92)  LDD0561  [2]
 LDCM0245  AC127 HCT 116 C76(0.76); C79(0.76)  LDD0562  [2]
 LDCM0259  AC14 HCT 116 C76(1.22); C79(1.22)  LDD0576  [2]
 LDCM0270  AC15 HCT 116 C76(1.30); C79(1.30)  LDD0587  [2]
 LDCM0281  AC21 HEK-293T C79(1.06)  LDD1520  [3]
 LDCM0284  AC24 HEK-293T C79(0.98)  LDD1523  [3]
 LDCM0285  AC25 HEK-293T C76(0.76); C79(0.76)  LDD0883  [2]
 LDCM0286  AC26 HEK-293T C76(0.79); C79(0.79)  LDD0884  [2]
 LDCM0287  AC27 HEK-293T C76(0.84); C79(0.84)  LDD0885  [2]
 LDCM0288  AC28 HEK-293T C76(0.67); C79(0.67)  LDD0886  [2]
 LDCM0289  AC29 HEK-293T C76(0.89); C79(0.89)  LDD0887  [2]
 LDCM0291  AC30 HEK-293T C76(0.86); C79(0.86)  LDD0889  [2]
 LDCM0292  AC31 HEK-293T C76(0.90); C79(0.90)  LDD0890  [2]
 LDCM0293  AC32 HEK-293T C76(0.88); C79(0.88)  LDD0891  [2]
 LDCM0294  AC33 HEK-293T C76(0.82); C79(0.82)  LDD0892  [2]
 LDCM0295  AC34 HEK-293T C76(0.81); C79(0.81)  LDD0893  [2]
 LDCM0298  AC37 HEK-293T C79(1.07)  LDD1537  [3]
 LDCM0302  AC40 HEK-293T C79(1.02)  LDD1541  [3]
 LDCM0307  AC45 HEK-293T C79(1.15)  LDD1546  [3]
 LDCM0308  AC46 HEK-293T C76(0.90); C79(0.90)  LDD0906  [2]
 LDCM0309  AC47 HEK-293T C76(1.00); C79(1.00)  LDD0907  [2]
 LDCM0310  AC48 HEK-293T C76(1.08); C79(1.08)  LDD0908  [2]
 LDCM0311  AC49 HEK-293T C76(1.00); C79(1.00)  LDD0909  [2]
 LDCM0312  AC5 HEK-293T C79(1.01)  LDD1551  [3]
 LDCM0313  AC50 HEK-293T C76(0.79); C79(0.79)  LDD0911  [2]
 LDCM0314  AC51 HEK-293T C76(0.96); C79(0.96)  LDD0912  [2]
 LDCM0315  AC52 HEK-293T C76(0.95); C79(0.95)  LDD0913  [2]
 LDCM0316  AC53 HEK-293T C76(0.77); C79(0.77)  LDD0914  [2]
 LDCM0317  AC54 HEK-293T C76(1.20); C79(1.20)  LDD0915  [2]
 LDCM0318  AC55 HEK-293T C76(0.94); C79(0.94)  LDD0916  [2]
 LDCM0319  AC56 HEK-293T C76(0.91); C79(0.91)  LDD0917  [2]
 LDCM0323  AC6 HCT 116 C76(1.40); C79(1.40)  LDD0640  [2]
 LDCM0325  AC61 HEK-293T C79(1.00)  LDD1564  [3]
 LDCM0328  AC64 HEK-293T C79(1.05)  LDD1567  [3]
 LDCM0332  AC68 HEK-293T C79(1.02)  LDD0930  [2]
 LDCM0333  AC69 HEK-293T C79(1.26)  LDD0931  [2]
 LDCM0334  AC7 HCT 116 C76(1.26); C79(1.26)  LDD0651  [2]
 LDCM0335  AC70 HEK-293T C79(0.99)  LDD0933  [2]
 LDCM0336  AC71 HEK-293T C79(1.27)  LDD0934  [2]
 LDCM0337  AC72 HEK-293T C79(1.72)  LDD0935  [2]
 LDCM0338  AC73 HEK-293T C79(1.38)  LDD0936  [2]
 LDCM0339  AC74 HEK-293T C79(1.13)  LDD0937  [2]
 LDCM0340  AC75 HEK-293T C79(1.27)  LDD0938  [2]
 LDCM0341  AC76 HEK-293T C79(1.23)  LDD0939  [2]
 LDCM0342  AC77 HEK-293T C79(1.08)  LDD0940  [2]
 LDCM0343  AC78 HEK-293T C79(1.07)  LDD0941  [2]
 LDCM0344  AC79 HEK-293T C79(1.11)  LDD0942  [2]
 LDCM0345  AC8 HCT 116 C76(1.40); C79(1.40)  LDD0662  [2]
 LDCM0346  AC80 HEK-293T C79(1.13)  LDD0944  [2]
 LDCM0347  AC81 HEK-293T C79(1.22)  LDD0945  [2]
 LDCM0348  AC82 HEK-293T C79(1.89)  LDD0946  [2]
 LDCM0248  AKOS034007472 HCT 116 C76(1.21); C79(1.21)  LDD0565  [2]
 LDCM0356  AKOS034007680 HCT 116 C76(1.51); C79(1.51)  LDD0673  [2]
 LDCM0275  AKOS034007705 HCT 116 C76(1.97); C79(1.97)  LDD0592  [2]
 LDCM0020  ARS-1620 HCC44 C76(1.02); C79(1.02)  LDD2171  [2]
 LDCM0367  CL1 HEK-293T C76(0.91); C79(0.91)  LDD0965  [2]
 LDCM0368  CL10 HEK-293T C76(0.79); C79(0.79)  LDD0966  [2]
 LDCM0369  CL100 HEK-293T C79(0.97)  LDD1573  [3]
 LDCM0370  CL101 HCT 116 C76(1.57); C79(1.57)  LDD0687  [2]
 LDCM0371  CL102 HCT 116 C76(1.43); C79(1.43)  LDD0688  [2]
 LDCM0372  CL103 HCT 116 C76(1.12); C79(1.12)  LDD0689  [2]
 LDCM0373  CL104 HCT 116 C76(1.16); C79(1.16)  LDD0690  [2]
 LDCM0377  CL108 HEK-293T C79(1.05)  LDD1581  [3]
 LDCM0379  CL11 HEK-293T C76(0.87); C79(0.87)  LDD0977  [2]
 LDCM0382  CL112 HEK-293T C76(0.81); C79(0.81)  LDD0980  [2]
 LDCM0383  CL113 HEK-293T C76(0.80); C79(0.80)  LDD0981  [2]
 LDCM0384  CL114 HEK-293T C76(0.70); C79(0.70)  LDD0982  [2]
 LDCM0385  CL115 HEK-293T C76(0.84); C79(0.84)  LDD0983  [2]
 LDCM0386  CL116 HEK-293T C76(0.70); C79(0.70)  LDD0984  [2]
 LDCM0390  CL12 HEK-293T C76(0.97); C79(0.97)  LDD0988  [2]
 LDCM0391  CL120 HEK-293T C79(1.17)  LDD1595  [3]
 LDCM0392  CL121 HEK-293T C76(1.00); C79(1.00)  LDD0990  [2]
 LDCM0393  CL122 HEK-293T C76(0.80); C79(0.80)  LDD0991  [2]
 LDCM0394  CL123 HEK-293T C76(0.77); C79(0.77)  LDD0992  [2]
 LDCM0395  CL124 HEK-293T C76(0.84); C79(0.84)  LDD0993  [2]
 LDCM0399  CL128 HEK-293T C79(1.01)  LDD1603  [3]
 LDCM0400  CL13 HEK-293T C76(0.86); C79(0.86)  LDD0998  [2]
 LDCM0401  CL14 HEK-293T C76(0.79); C79(0.79)  LDD0999  [2]
 LDCM0402  CL15 HEK-293T C76(0.82); C79(0.82)  LDD1000  [2]
 LDCM0403  CL16 HEK-293T C79(1.18)  LDD1607  [3]
 LDCM0407  CL2 HEK-293T C76(0.97); C79(0.97)  LDD1005  [2]
 LDCM0409  CL21 HEK-293T C79(1.22)  LDD1613  [3]
 LDCM0412  CL24 HEK-293T C79(1.15)  LDD1616  [3]
 LDCM0416  CL28 HEK-293T C79(1.06)  LDD1620  [3]
 LDCM0418  CL3 HEK-293T C76(1.14); C79(1.14)  LDD1016  [2]
 LDCM0422  CL33 HEK-293T C79(1.21)  LDD1626  [3]
 LDCM0425  CL36 HEK-293T C79(1.21)  LDD1629  [3]
 LDCM0429  CL4 HEK-293T C76(1.00); C79(1.00)  LDD1027  [2]
 LDCM0430  CL40 HEK-293T C79(1.09)  LDD1634  [3]
 LDCM0435  CL45 HEK-293T C79(1.16)  LDD1639  [3]
 LDCM0436  CL46 HCT 116 C76(1.10); C79(1.10)  LDD0753  [2]
 LDCM0437  CL47 HCT 116 C76(1.05); C79(1.05)  LDD0754  [2]
 LDCM0438  CL48 HCT 116 C76(0.98); C79(0.98)  LDD0755  [2]
 LDCM0439  CL49 HCT 116 C76(0.91); C79(0.91)  LDD0756  [2]
 LDCM0440  CL5 HEK-293T C76(0.99); C79(0.99)  LDD1038  [2]
 LDCM0441  CL50 HCT 116 C76(1.00); C79(1.00)  LDD0758  [2]
 LDCM0442  CL51 HCT 116 C76(0.91); C79(0.91)  LDD0759  [2]
 LDCM0443  CL52 HCT 116 C76(0.99); C79(0.99)  LDD0760  [2]
 LDCM0444  CL53 HCT 116 C76(0.89); C79(0.89)  LDD0761  [2]
 LDCM0445  CL54 HCT 116 C76(0.99); C79(0.99)  LDD0762  [2]
 LDCM0446  CL55 HCT 116 C76(1.09); C79(1.09)  LDD0763  [2]
 LDCM0447  CL56 HCT 116 C76(1.03); C79(1.03)  LDD0764  [2]
 LDCM0448  CL57 HCT 116 C76(0.97); C79(0.97)  LDD0765  [2]
 LDCM0449  CL58 HCT 116 C76(1.11); C79(1.11)  LDD0766  [2]
 LDCM0450  CL59 HCT 116 C76(0.96); C79(0.96)  LDD0767  [2]
 LDCM0451  CL6 HEK-293T C76(0.87); C79(0.87)  LDD1049  [2]
 LDCM0452  CL60 HCT 116 C76(1.19); C79(1.19)  LDD0769  [2]
 LDCM0453  CL61 HCT 116 C76(1.08); C79(1.08)  LDD0770  [2]
 LDCM0454  CL62 HCT 116 C76(1.11); C79(1.11)  LDD0771  [2]
 LDCM0455  CL63 HCT 116 C76(0.95); C79(0.95)  LDD0772  [2]
 LDCM0456  CL64 HCT 116 C76(1.17); C79(1.17)  LDD0773  [2]
 LDCM0457  CL65 HCT 116 C76(0.87); C79(0.87)  LDD0774  [2]
 LDCM0458  CL66 HCT 116 C76(0.96); C79(0.96)  LDD0775  [2]
 LDCM0459  CL67 HCT 116 C76(0.82); C79(0.82)  LDD0776  [2]
 LDCM0460  CL68 HCT 116 C76(0.90); C79(0.90)  LDD0777  [2]
 LDCM0461  CL69 HCT 116 C76(1.02); C79(1.02)  LDD0778  [2]
 LDCM0462  CL7 HEK-293T C76(0.89); C79(0.89)  LDD1060  [2]
 LDCM0463  CL70 HCT 116 C76(0.90); C79(0.90)  LDD0780  [2]
 LDCM0464  CL71 HCT 116 C76(0.90); C79(0.90)  LDD0781  [2]
 LDCM0465  CL72 HCT 116 C76(0.90); C79(0.90)  LDD0782  [2]
 LDCM0466  CL73 HCT 116 C76(0.95); C79(0.95)  LDD0783  [2]
 LDCM0467  CL74 HCT 116 C76(1.01); C79(1.01)  LDD0784  [2]
 LDCM0469  CL76 HCT 116 C76(0.79); C79(0.79)  LDD0786  [2]
 LDCM0470  CL77 HCT 116 C76(0.74); C79(0.74)  LDD0787  [2]
 LDCM0471  CL78 HCT 116 C76(0.77); C79(0.77)  LDD0788  [2]
 LDCM0472  CL79 HCT 116 C76(0.81); C79(0.81)  LDD0789  [2]
 LDCM0473  CL8 HEK-293T C76(1.11); C79(0.80)  LDD1071  [2]
 LDCM0474  CL80 HCT 116 C76(0.89); C79(0.89)  LDD0791  [2]
 LDCM0475  CL81 HCT 116 C76(0.70); C79(0.70)  LDD0792  [2]
 LDCM0476  CL82 HCT 116 C76(0.74); C79(0.74)  LDD0793  [2]
 LDCM0477  CL83 HCT 116 C76(0.80); C79(0.80)  LDD0794  [2]
 LDCM0478  CL84 HCT 116 C76(0.81); C79(0.81)  LDD0795  [2]
 LDCM0479  CL85 HCT 116 C76(0.83); C79(0.83)  LDD0796  [2]
 LDCM0480  CL86 HCT 116 C76(0.86); C79(0.86)  LDD0797  [2]
 LDCM0481  CL87 HCT 116 C76(0.72); C79(0.72)  LDD0798  [2]
 LDCM0482  CL88 HCT 116 C76(0.74); C79(0.74)  LDD0799  [2]
 LDCM0483  CL89 HCT 116 C76(0.86); C79(0.86)  LDD0800  [2]
 LDCM0484  CL9 HEK-293T C76(1.29); C79(0.79)  LDD1082  [2]
 LDCM0485  CL90 HCT 116 C76(0.69); C79(0.69)  LDD0802  [2]
 LDCM0488  CL93 HEK-293T C79(1.09)  LDD1691  [3]
 LDCM0491  CL96 HEK-293T C79(1.04)  LDD1694  [3]
 LDCM0468  Fragment33 HCT 116 C76(0.92); C79(0.92)  LDD0785  [2]
 LDCM0022  KB02 HCT 116 C76(1.63); C79(1.63)  LDD0080  [2]
 LDCM0023  KB03 HCT 116 C76(3.47); C79(3.47)  LDD0081  [2]
 LDCM0024  KB05 HCT 116 C76(1.45); C79(1.45)  LDD0082  [2]
 LDCM0021  THZ1 HCT 116 C76(1.02); C79(1.02)  LDD2173  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase RNF5 (RNF5) RNF5 family Q99942
Lysoplasmalogenase TMEM86A (TMEM86A) TMEM86 family Q8N2M4
Ubiquitin-conjugating enzyme E2 D1 (UBE2D1) Ubiquitin-conjugating enzyme family P51668
Ubiquitin-conjugating enzyme E2 D2 (UBE2D2) Ubiquitin-conjugating enzyme family P62837
Ubiquitin-conjugating enzyme E2 D4 (UBE2D4) Ubiquitin-conjugating enzyme family Q9Y2X8
Ubiquitin-conjugating enzyme E2 E2 (UBE2E2) Ubiquitin-conjugating enzyme family Q96LR5
Ubiquitin-conjugating enzyme E2 E3 (UBE2E3) Ubiquitin-conjugating enzyme family Q969T4
Ubiquitin-conjugating enzyme E2 K (UBE2K) Ubiquitin-conjugating enzyme family P61086
Lysosomal membrane ascorbate-dependent ferrireductase CYB561A3 (CYB561A3) . Q8NBI2
Transporter and channel
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Bax inhibitor 1 (TMBIM6) BI1 family P55061
Membrane-spanning 4-domains subfamily A member 13 (MS4A13) MS4A family Q5J8X5
Magnesium transporter NIPA4 (NIPAL4) NIPA family Q0D2K0
PRA1 family protein 3 (ARL6IP5) PRA1 family O75915
BOS complex subunit TMEM147 (TMEM147) TMEM147 family Q9BVK8
Protein YIPF2 (YIPF2) YIP1 family Q9BWQ6
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
CKLF-like MARVEL transmembrane domain-containing protein 5 (CMTM5) Chemokine-like factor family Q96DZ9
Other
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
MAL-like protein (MALL) MAL family Q13021
Myeloid-associated differentiation marker-like protein 2 (MYADML2) MAL family A6NDP7
Transmembrane protein 19 (TMEM19) TMEM19 family Q96HH6
Protein YIPF4 (YIPF4) YIP1 family Q9BSR8
Protein YIPF6 (YIPF6) YIP1 family Q96EC8

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402