Details of the Target

General Information of Target

Target ID LDTP08845
Target Name Xylosyl- and glucuronyltransferase LARGE2 (LARGE2)
Gene Name LARGE2
Gene ID 120071
Synonyms
GYLTL1B; Xylosyl- and glucuronyltransferase LARGE2; EC 2.4.-.-; Glycosyltransferase-like 1B; LARGE xylosyl- and glucuronyltransferase 2) [Includes: Alpha-1,3-xylosyltransferase LARGE2; EC 2.4.2.-; Beta-1,3-glucuronyltransferase LARGE2; EC 2.4.1.-)]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLPRGRPRALGAAALLLLLLLLGFLLFGGDLGCERREPGGRAGAPGCFPGPLMPRVPPDG
RLRRAAALDGDPGAGPGDHNRSDCGPQPPPPPKCELLHVAIVCAGHNSSRDVITLVKSML
FYRKNPLHLHLVTDAVARNILETLFHTWMVPAVRVSFYHADQLKPQVSWIPNKHYSGLYG
LMKLVLPSALPAELARVIVLDTDVTFASDISELWALFAHFSDTQAIGLVENQSDWYLGNL
WKNHRPWPALGRGFNTGVILLRLDRLRQAGWEQMWRLTARRELLSLPATSLADQDIFNAV
IKEHPGLVQRLPCVWNVQLSDHTLAERCYSEASDLKVIHWNSPKKLRVKNKHVEFFRNFY
LTFLEYDGNLLRRELFVCPSQPPPGAEQLQQALAQLDEEDPCFEFRQQQLTVHRVHVTFL
PHEPPPPRPHDVTLVAQLSMDRLQMLEALCRHWPGPMSLALYLTDAEAQQFLHFVEASPV
LAARQDVAYHVVYREGPLYPVNQLRNVALAQALTPYVFLSDIDFLPAYSLYDYLRASIEQ
LGLGSRRKAALVVPAFETLRYRFSFPHSKVELLALLDAGTLYTFRYHEWPRGHAPTDYAR
WREAQAPYRVQWAANYEPYVVVPRDCPRYDPRFVGFGWNKVAHIVELDAQEYELLVLPEA
FTIHLPHAPSLDISRFRSSPTYRDCLQALKDEFHQDLSRHHGAAALKYLPALQQPQSPAR
G
Target Bioclass
Enzyme
Family
Glycosyltransferase 49 family; Glycosyltransferase 8 family
Subcellular location
Golgi apparatus membrane
Function
Bifunctional glycosyltransferase with both alpha-1,3-xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the maturation of alpha-dystroglycan (DAG1) by glycosylation leading to DAG1 binding to laminin G-like domain-containing extracellular proteins with high affinity and in a phosphorylated-O-mannosyl trisaccharide dependent manner. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Supports the maturation of DAG1 more effectively than LARGE1. In addition, can modify both heparan sulfate (HS)- and chondroitin/dermatan sulfate (CS/DS)-proteoglycans (PGs), namely GPC4, with a glycosaminoglycan (GAG)-like polysaccharide composed of xylose and glucuronic acid to confer laminin binding.
Uniprot ID
Q8N3Y3
Ensemble ID
ENST00000325468.9
HGNC ID
HGNC:16522

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C328(1.01)  LDD0532  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HCT 116 C328(1.01)  LDD0532  [1]
 LDCM0226  AC11 HCT 116 C328(1.03)  LDD0543  [1]
 LDCM0230  AC113 HCT 116 C328(0.96)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C328(0.99)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C328(0.98)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C328(1.28)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C328(0.96)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C328(1.16)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C328(1.21)  LDD0553  [1]
 LDCM0237  AC12 HCT 116 C328(1.08)  LDD0554  [1]
 LDCM0238  AC120 HCT 116 C328(0.87)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C328(0.95)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C328(1.17)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C328(0.83)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C328(0.87)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C328(1.01)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C328(1.18)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C328(0.94)  LDD0562  [1]
 LDCM0259  AC14 HCT 116 C328(0.83)  LDD0576  [1]
 LDCM0263  AC143 HCT 116 C328(0.88)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C328(1.20)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C328(1.00)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C328(1.28)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C328(1.31)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C328(1.44)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C328(1.52)  LDD0586  [1]
 LDCM0270  AC15 HCT 116 C328(0.97)  LDD0587  [1]
 LDCM0271  AC150 HCT 116 C328(0.92)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C328(0.96)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C328(1.20)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C328(1.52)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C328(1.18)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C328(1.08)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C328(1.01)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C328(0.95)  LDD2161  [1]
 LDCM0320  AC57 HCT 116 C328(1.15)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C328(1.24)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C328(1.29)  LDD0639  [1]
 LDCM0323  AC6 HCT 116 C328(0.98)  LDD0640  [1]
 LDCM0324  AC60 HCT 116 C328(1.35)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C328(1.16)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C328(1.25)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C328(1.05)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C328(1.53)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C328(1.64)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C328(1.67)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C328(1.57)  LDD0648  [1]
 LDCM0332  AC68 HCT 116 C328(1.10)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C328(1.01)  LDD0650  [1]
 LDCM0334  AC7 HCT 116 C328(0.94)  LDD0651  [1]
 LDCM0335  AC70 HCT 116 C328(1.06)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C328(1.22)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C328(0.98)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C328(1.25)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C328(1.23)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C328(1.25)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C328(1.18)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C328(1.28)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C328(1.04)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C328(1.06)  LDD0661  [1]
 LDCM0345  AC8 HCT 116 C328(1.06)  LDD0662  [1]
 LDCM0346  AC80 HCT 116 C328(1.13)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C328(0.98)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C328(1.16)  LDD0665  [1]
 LDCM0248  AKOS034007472 HCT 116 C328(0.90)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C328(0.96)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C328(1.16)  LDD0592  [1]
 LDCM0370  CL101 HCT 116 C328(1.06)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C328(1.07)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C328(0.99)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C328(1.03)  LDD0690  [1]
 LDCM0396  CL125 HCT 116 C328(1.15)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C328(1.14)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C328(1.06)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C328(1.28)  LDD0716  [1]
 LDCM0403  CL16 HCT 116 C328(1.07)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C328(1.20)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C328(0.98)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C328(0.97)  LDD0723  [1]
 LDCM0408  CL20 HCT 116 C328(1.17)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C328(1.02)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C328(1.02)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C328(0.95)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C328(0.96)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C328(1.10)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C328(0.90)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C328(1.00)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C328(1.07)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C328(1.07)  LDD0734  [1]
 LDCM0419  CL30 HCT 116 C328(0.95)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C328(0.96)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C328(1.07)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C328(1.27)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C328(1.28)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C328(1.33)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C328(1.21)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C328(1.25)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C328(1.05)  LDD0745  [1]
 LDCM0430  CL40 HCT 116 C328(1.03)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C328(1.15)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C328(1.25)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C328(1.24)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C328(1.38)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C328(1.34)  LDD0752  [1]
 LDCM0427  Fragment51 HCT 116 C328(1.16)  LDD0744  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.