Details of the Target

General Information of Target

Target ID LDTP06144
Target Name Equilibrative nucleoside transporter 2 (SLC29A2)
Gene Name SLC29A2
Gene ID 3177
Synonyms
DER12; ENT2; HNP36; Equilibrative nucleoside transporter 2; hENT2; 36 kDa nucleolar protein HNP36; Delayed-early response protein 12; Equilibrative nitrobenzylmercaptopurine riboside-insensitive nucleoside transporter; Equilibrative NBMPR-insensitive nucleoside transporter; Hydrophobic nucleolar protein, 36 kDa; Nucleoside transporter, ei-type; Solute carrier family 29 member 2
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MARGDAPRDSYHLVGISFFILGLGTLLPWNFFITAIPYFQARLAGAGNSTARILSTNHTG
PEDAFNFNNWVTLLSQLPLLLFTLLNSFLYQCVPETVRILGSLLAILLLFALTAALVKVD
MSPGPFFSITMASVCFINSFSAVLQGSLFGQLGTMPSTYSTLFLSGQGLAGIFAALAMLL
SMASGVDAETSALGYFITPCVGILMSIVCYLSLPHLKFARYYLANKSSQAQAQELETKAE
LLQSDENGIPSSPQKVALTLDLDLEKEPESEPDEPQKPGKPSVFTVFQKIWLTALCLVLV
FTVTLSVFPAITAMVTSSTSPGKWSQFFNPICCFLLFNIMDWLGRSLTSYFLWPDEDSRL
LPLLVCLRFLFVPLFMLCHVPQRSRLPILFPQDAYFITFMLLFAVSNGYLVSLTMCLAPR
QVLPHEREVAGALMTFFLALGLSCGASLSFLFKALL
Target Bioclass
Transporter and channel
Family
SLC29A/ENT transporter (TC 2.A.57) family
Subcellular location
Basolateral cell membrane
Function
Bidirectional uniporter involved in the facilitative transport of nucleosides and nucleobases, and contributes to maintaining their cellular homeostasis. Functions as a Na(+)-independent, passive transporter. Involved in the transport of nucleosides such as inosine, adenosine, uridine, thymidine, cytidine and guanosine. Also able to transport purine nucleobases (hypoxanthine, adenine, guanine) and pyrimidine nucleobases (thymine, uracil). Involved in nucleoside transport at basolateral membrane of kidney cells, allowing liver absorption of nucleoside metabolites. Mediates apical nucleoside uptake into Sertoli cells, thereby regulating the transport of nucleosides in testis across the blood-testis-barrier. Mediates both the influx and efflux of hypoxanthine in skeletal muscle microvascular endothelial cells to control the amount of intracellular hypoxanthine available for xanthine oxidase-mediated ROS production.; [Isoform 3]: Non functional nucleoside transporter protein for adenosine or thymidine transport. Does not express on cell membrane.
Uniprot ID
Q14542
Ensemble ID
ENST00000311161.11
HGNC ID
HGNC:11004
ChEMBL ID
CHEMBL3509606

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
LS123 SNV: p.L116V .
PF382 SNV: p.S244F .
TOV21G SNV: p.P391Q .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
Dasatinib-CA-4PAP
 Probe Info  		9.25  LDD0270  [1]
IA-alkyne
 Probe Info  		C366(0.67); C378(1.08)  LDD2182  [2]
DBIA
 Probe Info  		C378(1.30); C366(0.82)  LDD1507  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C378(1.30); C366(0.82)  LDD1507  [3]
 LDCM0215  AC10 HEK-293T C378(1.01); C366(1.15)  LDD1508  [3]
 LDCM0237  AC12 HEK-293T C378(1.19)  LDD1510  [3]
 LDCM0259  AC14 HEK-293T C378(1.26); C366(1.12)  LDD1512  [3]
 LDCM0270  AC15 HEK-293T C378(1.25)  LDD1513  [3]
 LDCM0276  AC17 HEK-293T C378(0.98); C366(0.77)  LDD1515  [3]
 LDCM0277  AC18 HEK-293T C378(0.82); C366(0.82)  LDD1516  [3]
 LDCM0279  AC2 HEK-293T C378(1.20); C366(1.10)  LDD1518  [3]
 LDCM0280  AC20 HEK-293T C378(1.07)  LDD1519  [3]
 LDCM0281  AC21 HEK-293T C366(0.97)  LDD1520  [3]
 LDCM0282  AC22 HEK-293T C378(1.97); C366(0.67)  LDD1521  [3]
 LDCM0283  AC23 HEK-293T C378(1.02)  LDD1522  [3]
 LDCM0284  AC24 HEK-293T C366(0.84)  LDD1523  [3]
 LDCM0285  AC25 HEK-293T C378(1.22); C366(0.94)  LDD1524  [3]
 LDCM0286  AC26 HEK-293T C378(1.11); C366(0.95)  LDD1525  [3]
 LDCM0288  AC28 HEK-293T C378(1.17)  LDD1527  [3]
 LDCM0289  AC29 HEK-293T C366(0.95)  LDD1528  [3]
 LDCM0291  AC30 HEK-293T C378(1.28); C366(0.74)  LDD1530  [3]
 LDCM0292  AC31 HEK-293T C378(1.05)  LDD1531  [3]
 LDCM0293  AC32 HEK-293T C366(1.12)  LDD1532  [3]
 LDCM0294  AC33 HEK-293T C378(0.84); C366(0.80)  LDD1533  [3]
 LDCM0295  AC34 HEK-293T C378(0.98); C366(0.92)  LDD1534  [3]
 LDCM0297  AC36 HEK-293T C378(1.15)  LDD1536  [3]
 LDCM0298  AC37 HEK-293T C366(0.95)  LDD1537  [3]
 LDCM0299  AC38 HEK-293T C378(1.15); C366(1.01)  LDD1538  [3]
 LDCM0300  AC39 HEK-293T C378(1.13)  LDD1539  [3]
 LDCM0301  AC4 HEK-293T C378(1.25)  LDD1540  [3]
 LDCM0302  AC40 HEK-293T C366(0.75)  LDD1541  [3]
 LDCM0303  AC41 HEK-293T C378(1.28); C366(0.80)  LDD1542  [3]
 LDCM0304  AC42 HEK-293T C378(1.18); C366(1.13)  LDD1543  [3]
 LDCM0306  AC44 HEK-293T C378(1.12)  LDD1545  [3]
 LDCM0307  AC45 HEK-293T C366(1.01)  LDD1546  [3]
 LDCM0308  AC46 HEK-293T C378(1.48); C366(0.91)  LDD1547  [3]
 LDCM0309  AC47 HEK-293T C378(1.12)  LDD1548  [3]
 LDCM0310  AC48 HEK-293T C366(0.86)  LDD1549  [3]
 LDCM0311  AC49 HEK-293T C378(0.77); C366(0.78)  LDD1550  [3]
 LDCM0312  AC5 HEK-293T C366(0.83)  LDD1551  [3]
 LDCM0313  AC50 HEK-293T C378(1.11); C366(0.96)  LDD1552  [3]
 LDCM0315  AC52 HEK-293T C378(1.22)  LDD1554  [3]
 LDCM0316  AC53 HEK-293T C366(0.71)  LDD1555  [3]
 LDCM0317  AC54 HEK-293T C378(1.53); C366(0.68)  LDD1556  [3]
 LDCM0318  AC55 HEK-293T C378(1.08)  LDD1557  [3]
 LDCM0319  AC56 HEK-293T C366(1.16)  LDD1558  [3]
 LDCM0320  AC57 HEK-293T C378(1.11); C366(0.92)  LDD1559  [3]
 LDCM0321  AC58 HEK-293T C378(1.28); C366(1.04)  LDD1560  [3]
 LDCM0323  AC6 HEK-293T C378(1.26); C366(0.81)  LDD1562  [3]
 LDCM0324  AC60 HEK-293T C378(1.07)  LDD1563  [3]
 LDCM0325  AC61 HEK-293T C366(1.06)  LDD1564  [3]
 LDCM0326  AC62 HEK-293T C378(1.63); C366(0.98)  LDD1565  [3]
 LDCM0327  AC63 HEK-293T C378(1.12)  LDD1566  [3]
 LDCM0328  AC64 HEK-293T C366(1.02)  LDD1567  [3]
 LDCM0334  AC7 HEK-293T C378(1.00)  LDD1568  [3]
 LDCM0345  AC8 HEK-293T C366(0.88)  LDD1569  [3]
 LDCM0248  AKOS034007472 HEK-293T C366(0.75)  LDD1511  [3]
 LDCM0356  AKOS034007680 HEK-293T C378(1.22); C366(0.86)  LDD1570  [3]
 LDCM0275  AKOS034007705 HEK-293T C366(0.96)  LDD1514  [3]
 LDCM0367  CL1 HEK-293T C378(1.21)  LDD1571  [3]
 LDCM0368  CL10 HEK-293T C378(1.45); C366(0.87)  LDD1572  [3]
 LDCM0369  CL100 HEK-293T C378(1.08); C366(1.08)  LDD1573  [3]
 LDCM0370  CL101 HEK-293T C378(0.76)  LDD1574  [3]
 LDCM0371  CL102 HEK-293T C378(1.10); C366(0.82)  LDD1575  [3]
 LDCM0373  CL104 HEK-293T C378(1.03); C366(1.04)  LDD1577  [3]
 LDCM0374  CL105 HEK-293T C378(1.40)  LDD1578  [3]
 LDCM0375  CL106 HEK-293T C378(1.05); C366(0.77)  LDD1579  [3]
 LDCM0377  CL108 HEK-293T C378(1.02); C366(0.92)  LDD1581  [3]
 LDCM0378  CL109 HEK-293T C378(1.01)  LDD1582  [3]
 LDCM0379  CL11 HEK-293T C378(1.20)  LDD1583  [3]
 LDCM0380  CL110 HEK-293T C378(1.13); C366(0.74)  LDD1584  [3]
 LDCM0382  CL112 HEK-293T C378(1.03); C366(1.04)  LDD1586  [3]
 LDCM0383  CL113 HEK-293T C378(0.84)  LDD1587  [3]
 LDCM0384  CL114 HEK-293T C378(1.17); C366(0.72)  LDD1588  [3]
 LDCM0386  CL116 HEK-293T C378(0.93); C366(0.99)  LDD1590  [3]
 LDCM0387  CL117 HEK-293T C378(0.85)  LDD1591  [3]
 LDCM0388  CL118 HEK-293T C378(1.03); C366(0.79)  LDD1592  [3]
 LDCM0390  CL12 HEK-293T C366(1.08)  LDD1594  [3]
 LDCM0391  CL120 HEK-293T C378(0.97); C366(0.84)  LDD1595  [3]
 LDCM0392  CL121 HEK-293T C378(1.20)  LDD1596  [3]
 LDCM0393  CL122 HEK-293T C378(1.07); C366(0.73)  LDD1597  [3]
 LDCM0395  CL124 HEK-293T C378(0.96); C366(1.01)  LDD1599  [3]
 LDCM0396  CL125 HEK-293T C378(0.82)  LDD1600  [3]
 LDCM0397  CL126 HEK-293T C378(1.22); C366(0.86)  LDD1601  [3]
 LDCM0399  CL128 HEK-293T C378(1.06); C366(0.86)  LDD1603  [3]
 LDCM0400  CL13 HEK-293T C378(1.38)  LDD1604  [3]
 LDCM0401  CL14 HEK-293T C378(1.16); C366(0.80)  LDD1605  [3]
 LDCM0403  CL16 HEK-293T C378(0.98); C366(1.09)  LDD1607  [3]
 LDCM0404  CL17 HEK-293T C378(1.36); C366(0.88)  LDD1608  [3]
 LDCM0405  CL18 HEK-293T C378(1.14); C366(1.14)  LDD1609  [3]
 LDCM0407  CL2 HEK-293T C378(1.25); C366(0.89)  LDD1611  [3]
 LDCM0408  CL20 HEK-293T C378(1.18)  LDD1612  [3]
 LDCM0409  CL21 HEK-293T C366(0.68)  LDD1613  [3]
 LDCM0410  CL22 HEK-293T C378(0.99); C366(0.75)  LDD1614  [3]
 LDCM0411  CL23 HEK-293T C378(0.98)  LDD1615  [3]
 LDCM0412  CL24 HEK-293T C366(1.00)  LDD1616  [3]
 LDCM0413  CL25 HEK-293T C378(1.65)  LDD1617  [3]
 LDCM0414  CL26 HEK-293T C378(1.13); C366(0.72)  LDD1618  [3]
 LDCM0416  CL28 HEK-293T C378(0.99); C366(1.14)  LDD1620  [3]
 LDCM0417  CL29 HEK-293T C378(0.75); C366(0.90)  LDD1621  [3]
 LDCM0419  CL30 HEK-293T C378(0.88); C366(0.74)  LDD1623  [3]
 LDCM0421  CL32 HEK-293T C378(1.02)  LDD1625  [3]
 LDCM0422  CL33 HEK-293T C366(1.33)  LDD1626  [3]
 LDCM0423  CL34 HEK-293T C378(1.31); C366(0.80)  LDD1627  [3]
 LDCM0424  CL35 HEK-293T C378(0.98)  LDD1628  [3]
 LDCM0425  CL36 HEK-293T C366(0.81)  LDD1629  [3]
 LDCM0426  CL37 HEK-293T C378(0.97)  LDD1630  [3]
 LDCM0429  CL4 HEK-293T C378(1.24); C366(0.82)  LDD1633  [3]
 LDCM0430  CL40 HEK-293T C378(0.97); C366(1.10)  LDD1634  [3]
 LDCM0431  CL41 HEK-293T C378(1.16); C366(0.94)  LDD1635  [3]
 LDCM0432  CL42 HEK-293T C378(0.96); C366(0.96)  LDD1636  [3]
 LDCM0434  CL44 HEK-293T C378(1.13)  LDD1638  [3]
 LDCM0435  CL45 HEK-293T C366(0.78)  LDD1639  [3]
 LDCM0436  CL46 HEK-293T C378(1.19); C366(0.87)  LDD1640  [3]
 LDCM0437  CL47 HEK-293T C378(0.98)  LDD1641  [3]
 LDCM0438  CL48 HEK-293T C366(0.91)  LDD1642  [3]
 LDCM0439  CL49 HEK-293T C378(1.08)  LDD1643  [3]
 LDCM0440  CL5 HEK-293T C378(1.09); C366(0.97)  LDD1644  [3]
 LDCM0441  CL50 HEK-293T C378(1.27); C366(1.00)  LDD1645  [3]
 LDCM0443  CL52 HEK-293T C378(1.08); C366(1.09)  LDD1646  [3]
 LDCM0444  CL53 HEK-293T C378(0.89); C366(1.13)  LDD1647  [3]
 LDCM0445  CL54 HEK-293T C378(1.00); C366(1.00)  LDD1648  [3]
 LDCM0447  CL56 HEK-293T C378(0.94)  LDD1650  [3]
 LDCM0448  CL57 HEK-293T C366(0.79)  LDD1651  [3]
 LDCM0449  CL58 HEK-293T C378(1.93); C366(0.87)  LDD1652  [3]
 LDCM0450  CL59 HEK-293T C378(1.12)  LDD1653  [3]
 LDCM0451  CL6 HEK-293T C378(1.20); C366(0.77)  LDD1654  [3]
 LDCM0452  CL60 HEK-293T C366(0.88)  LDD1655  [3]
 LDCM0453  CL61 HEK-293T C378(1.52)  LDD1656  [3]
 LDCM0454  CL62 HEK-293T C378(1.22); C366(0.82)  LDD1657  [3]
 LDCM0456  CL64 HEK-293T C378(1.16); C366(0.88)  LDD1659  [3]
 LDCM0457  CL65 HEK-293T C378(0.90); C366(0.92)  LDD1660  [3]
 LDCM0458  CL66 HEK-293T C378(1.01); C366(1.08)  LDD1661  [3]
 LDCM0460  CL68 HEK-293T C378(0.77)  LDD1663  [3]
 LDCM0461  CL69 HEK-293T C366(0.73)  LDD1664  [3]
 LDCM0463  CL70 HEK-293T C378(1.58); C366(1.04)  LDD1666  [3]
 LDCM0464  CL71 HEK-293T C378(0.90)  LDD1667  [3]
 LDCM0465  CL72 HEK-293T C366(0.92)  LDD1668  [3]
 LDCM0466  CL73 HEK-293T C378(0.95)  LDD1669  [3]
 LDCM0467  CL74 HEK-293T C378(0.98); C366(0.73)  LDD1670  [3]
 LDCM0469  CL76 HEK-293T C378(0.93); C366(0.90)  LDD1672  [3]
 LDCM0470  CL77 HEK-293T C378(1.29); C366(0.84)  LDD1673  [3]
 LDCM0471  CL78 HEK-293T C378(1.09); C366(0.94)  LDD1674  [3]
 LDCM0473  CL8 HEK-293T C378(1.34)  LDD1676  [3]
 LDCM0474  CL80 HEK-293T C378(1.09)  LDD1677  [3]
 LDCM0475  CL81 HEK-293T C366(1.13)  LDD1678  [3]
 LDCM0476  CL82 HEK-293T C378(1.19); C366(0.74)  LDD1679  [3]
 LDCM0477  CL83 HEK-293T C378(1.10)  LDD1680  [3]
 LDCM0478  CL84 HEK-293T C366(0.98)  LDD1681  [3]
 LDCM0479  CL85 HEK-293T C378(1.48)  LDD1682  [3]
 LDCM0480  CL86 HEK-293T C378(1.30); C366(0.89)  LDD1683  [3]
 LDCM0482  CL88 HEK-293T C378(1.06); C366(1.09)  LDD1685  [3]
 LDCM0483  CL89 HEK-293T C378(0.88); C366(0.83)  LDD1686  [3]
 LDCM0484  CL9 HEK-293T C366(1.01)  LDD1687  [3]
 LDCM0485  CL90 HEK-293T C378(1.25); C366(1.27)  LDD1688  [3]
 LDCM0487  CL92 HEK-293T C378(1.01)  LDD1690  [3]
 LDCM0488  CL93 HEK-293T C366(0.79)  LDD1691  [3]
 LDCM0489  CL94 HEK-293T C378(1.39); C366(0.69)  LDD1692  [3]
 LDCM0490  CL95 HEK-293T C378(1.25)  LDD1693  [3]
 LDCM0491  CL96 HEK-293T C366(0.89)  LDD1694  [3]
 LDCM0492  CL97 HEK-293T C378(1.01)  LDD1695  [3]
 LDCM0493  CL98 HEK-293T C378(0.92); C366(0.87)  LDD1696  [3]
 LDCM0097  Dasatinib K562 9.25  LDD0270  [1]
 LDCM0625  F8 Ramos C366(0.96); C378(3.22)  LDD2187  [2]
 LDCM0572  Fragment10 Ramos C366(0.77)  LDD2189  [2]
 LDCM0573  Fragment11 Ramos C366(1.27); C378(16.88)  LDD2190  [2]
 LDCM0574  Fragment12 Ramos C366(1.25)  LDD2191  [2]
 LDCM0575  Fragment13 Ramos C366(0.70)  LDD2192  [2]
 LDCM0576  Fragment14 Ramos C366(5.97)  LDD2193  [2]
 LDCM0579  Fragment20 Ramos C366(1.11)  LDD2194  [2]
 LDCM0580  Fragment21 Ramos C366(1.06)  LDD2195  [2]
 LDCM0582  Fragment23 Ramos C366(1.00)  LDD2196  [2]
 LDCM0578  Fragment27 Ramos C366(0.67)  LDD2197  [2]
 LDCM0586  Fragment28 Ramos C366(1.20)  LDD2198  [2]
 LDCM0588  Fragment30 Ramos C366(0.69)  LDD2199  [2]
 LDCM0589  Fragment31 Ramos C366(1.04)  LDD2200  [2]
 LDCM0590  Fragment32 Ramos C366(0.71)  LDD2201  [2]
 LDCM0468  Fragment33 Ramos C366(0.59)  LDD2202  [2]
 LDCM0596  Fragment38 Ramos C366(1.34)  LDD2203  [2]
 LDCM0566  Fragment4 Ramos C366(0.99)  LDD2184  [2]
 LDCM0427  Fragment51 HEK-293T C378(0.98); C366(0.76)  LDD1631  [3]
 LDCM0610  Fragment52 Ramos C366(1.37)  LDD2204  [2]
 LDCM0614  Fragment56 Ramos C366(0.61)  LDD2205  [2]
 LDCM0569  Fragment7 Ramos C366(0.80); C378(2.06)  LDD2186  [2]
 LDCM0571  Fragment9 Ramos C366(1.56)  LDD2188  [2]
 LDCM0022  KB02 Ramos C366(0.67); C378(1.08)  LDD2182  [2]
 LDCM0024  KB05 Ramos C366(2.77)  LDD2185  [2]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 10 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Adenosine Small molecular drug DB00640
Azathioprine Small molecular drug DB00993
Didanosine Small molecular drug DB00900
Ethanol Small molecular drug DB00898
Gemcitabine Small molecular drug DB00441
Mercaptopurine Small molecular drug DB01033
Tegafur-uracil Small molecular drug DB09327
Trifluridine Small molecular drug DB00432
Zalcitabine Small molecular drug DB00943
Zidovudine Small molecular drug DB00495

References

1 Streamlined Target Deconvolution Approach Utilizing a Single Photoreactive Chloroalkane Capture Tag. ACS Chem Biol. 2021 Feb 19;16(2):404-413. doi: 10.1021/acschembio.0c00987. Epub 2021 Feb 5.
2 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402