Details of the Target

General Information of Target

Target ID LDTP05853
Target Name Ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1)
Gene Name UBE2V1
Gene ID 387522
Synonyms
CROC1; UBE2V; UEV1; Ubiquitin-conjugating enzyme E2 variant 1; UEV-1; CROC-1; TRAF6-regulated IKK activator 1 beta Uev1A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAATTGSGVKVPRNFRLLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIY
ENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVV
LQELRRLMMSKENMKLPQPPEGQCYSN
Target Bioclass
Enzyme
Family
Ubiquitin-conjugating enzyme family
Subcellular location
Nucleus
Function
Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UBE2N, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway.
Uniprot ID
Q13404
Ensemble ID
ENST00000340309.7
HGNC ID
HGNC:12494

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 24 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
FBP2
 Probe Info  		2.34  LDD0323  [2]
YN-1
 Probe Info  		100.00  LDD0444  [3]
BTD
 Probe Info  		C71(0.57)  LDD1699  [4]
AZ-9
 Probe Info  		E22(10.00); E19(10.00)  LDD2209  [5]
Probe 1
 Probe Info  		Y60(64.44); Y75(11.99); Y115(80.34)  LDD3495  [6]
DBIA
 Probe Info  		C94(2.77); C218(1.55)  LDD3310  [7]
HHS-475
 Probe Info  		Y115(0.92)  LDD0264  [8]
HHS-465
 Probe Info  		Y115(10.00)  LDD2237  [9]
ATP probe
 Probe Info  		K74(0.00); K131(0.00)  LDD0199  [10]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [11]
IA-alkyne
 Probe Info  		C144(0.00); C71(0.00)  LDD0036  [11]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [11]
ATP probe
 Probe Info  		N.A.  LDD0035  [12]
TFBX
 Probe Info  		C71(0.00); C144(0.00)  LDD0027  [13]
1d-yne
 Probe Info  		N.A.  LDD0356  [14]
IPM
 Probe Info  		N.A.  LDD2156  [15]
SF
 Probe Info  		N.A.  LDD0028  [16]
STPyne
 Probe Info  		N.A.  LDD0009  [17]
1c-yne
 Probe Info  		N.A.  LDD0228  [14]
Acrolein
 Probe Info  		C71(0.00); C144(0.00)  LDD0217  [18]
Methacrolein
 Probe Info  		N.A.  LDD0218  [18]
W1
 Probe Info  		N.A.  LDD0236  [19]
NAIA_5
 Probe Info  		C144(0.00); C71(0.00)  LDD2223  [20]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C71(0.23)  LDD2142  [4]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C71(0.37)  LDD2112  [4]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C71(0.47)  LDD2095  [4]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C71(1.00)  LDD2130  [4]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C71(0.99)  LDD2117  [4]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C71(1.23)  LDD2152  [4]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C71(0.46)  LDD2132  [4]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C71(0.80)  LDD2131  [4]
 LDCM0545  Acetamide MDA-MB-231 C71(0.74)  LDD2138  [4]
 LDCM0520  AKOS000195272 MDA-MB-231 C71(0.51)  LDD2113  [4]
 LDCM0156  Aniline NCI-H1299 13.19  LDD0403  [1]
 LDCM0498  BS-3668 MDA-MB-231 C71(0.65); C144(0.79)  LDD2091  [4]
 LDCM0108  Chloroacetamide HeLa C71(0.00); C144(0.00)  LDD0222  [18]
 LDCM0632  CL-Sc Hep-G2 C144(20.00); C71(0.53)  LDD2227  [20]
 LDCM0625  F8 Ramos C144(0.93)  LDD2187  [21]
 LDCM0572  Fragment10 Ramos C144(0.53)  LDD2189  [21]
 LDCM0573  Fragment11 Ramos C144(0.05)  LDD2190  [21]
 LDCM0574  Fragment12 Ramos C144(0.55)  LDD2191  [21]
 LDCM0575  Fragment13 Ramos C144(1.04)  LDD2192  [21]
 LDCM0576  Fragment14 Ramos C144(0.85)  LDD2193  [21]
 LDCM0579  Fragment20 Ramos C144(0.42)  LDD2194  [21]
 LDCM0580  Fragment21 Ramos C144(0.95)  LDD2195  [21]
 LDCM0582  Fragment23 Ramos C144(0.81)  LDD2196  [21]
 LDCM0578  Fragment27 Ramos C144(1.22)  LDD2197  [21]
 LDCM0588  Fragment30 Ramos C144(1.38)  LDD2199  [21]
 LDCM0589  Fragment31 Ramos C144(1.44)  LDD2200  [21]
 LDCM0590  Fragment32 Ramos C144(0.64)  LDD2201  [21]
 LDCM0468  Fragment33 Ramos C144(1.31)  LDD2202  [21]
 LDCM0596  Fragment38 Ramos C144(0.68)  LDD2203  [21]
 LDCM0566  Fragment4 Ramos C144(0.63)  LDD2184  [21]
 LDCM0614  Fragment56 Ramos C144(1.19)  LDD2205  [21]
 LDCM0569  Fragment7 Ramos C144(0.49)  LDD2186  [21]
 LDCM0571  Fragment9 Ramos C144(0.43)  LDD2188  [21]
 LDCM0116  HHS-0101 DM93 Y115(0.92)  LDD0264  [8]
 LDCM0117  HHS-0201 DM93 Y115(0.85)  LDD0265  [8]
 LDCM0118  HHS-0301 DM93 Y115(0.89)  LDD0266  [8]
 LDCM0119  HHS-0401 DM93 Y115(0.96)  LDD0267  [8]
 LDCM0120  HHS-0701 DM93 Y115(0.95)  LDD0268  [8]
 LDCM0107  IAA HeLa N.A.  LDD0221  [18]
 LDCM0022  KB02 HEK-293T C144(1.03)  LDD1492  [22]
 LDCM0023  KB03 HEK-293T C144(0.94)  LDD1497  [22]
 LDCM0024  KB05 COLO792 C94(2.77); C218(1.55)  LDD3310  [7]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C71(1.47)  LDD2102  [4]
 LDCM0109  NEM HeLa N.A.  LDD0223  [18]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C144(0.58)  LDD2089  [4]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C71(1.12)  LDD2090  [4]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C71(0.90)  LDD2093  [4]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C71(2.42)  LDD2094  [4]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C71(0.07)  LDD2096  [4]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C71(0.61)  LDD2097  [4]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C71(0.88)  LDD2098  [4]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C71(1.01)  LDD2099  [4]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C71(0.33)  LDD2100  [4]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C71(0.83)  LDD2101  [4]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C71(0.31)  LDD2104  [4]
 LDCM0513  Nucleophilic fragment 19b MDA-MB-231 C71(0.16)  LDD2106  [4]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C71(0.87)  LDD2107  [4]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C71(0.36)  LDD2108  [4]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C71(0.40)  LDD2109  [4]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C71(0.88)  LDD2111  [4]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C71(0.64)  LDD2114  [4]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C71(0.33)  LDD2115  [4]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C71(0.31)  LDD2116  [4]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C71(0.24)  LDD2118  [4]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C71(2.21)  LDD2119  [4]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C71(0.88)  LDD2120  [4]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C71(0.12)  LDD2122  [4]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C71(0.12)  LDD2124  [4]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C71(0.95)  LDD2125  [4]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C71(0.09)  LDD2126  [4]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C71(0.87)  LDD2127  [4]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C71(1.01)  LDD2128  [4]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C71(0.89)  LDD2129  [4]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C71(0.32)  LDD2134  [4]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C71(1.04)  LDD2135  [4]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C71(0.90)  LDD2136  [4]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C71(0.92)  LDD2137  [4]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C71(2.11)  LDD1700  [4]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C71(0.68)  LDD2140  [4]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C71(0.67)  LDD2141  [4]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C71(1.04)  LDD2143  [4]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C71(3.07)  LDD2144  [4]
 LDCM0551  Nucleophilic fragment 5b MDA-MB-231 C71(0.11)  LDD2145  [4]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C71(0.72)  LDD2146  [4]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C71(0.38)  LDD2148  [4]
 LDCM0555  Nucleophilic fragment 7b MDA-MB-231 C71(0.11)  LDD2149  [4]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C71(0.58)  LDD2150  [4]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C71(0.11)  LDD2151  [4]
 LDCM0131  RA190 MM1.R C144(1.04)  LDD0304  [23]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
E3 ubiquitin-protein ligase Arkadia (RNF111) Arkadia family Q6ZNA4
Endoplasmic reticulum chaperone BiP (HSPA5) Heat shock protein 70 family P11021
E3 ubiquitin-protein ligase XIAP (XIAP) IAP family P98170
Kallikrein-6 (KLK6) Peptidase S1 family Q92876
Fibroblast growth factor receptor 3 (FGFR3) Tyr protein kinase family P22607
E3 ubiquitin-protein ligase TRIM32 (TRIM32) TRIM/RBCC family Q13049
Ubiquitin-conjugating enzyme E2 N (UBE2N) Ubiquitin-conjugating enzyme family P61088
E3 ubiquitin-protein ligase ZNRF1 (ZNRF1) . Q8ND25
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Glutamate receptor ionotropic, NMDA 2C (GRIN2C) Glutamate-gated ion channel family Q14957
Alpha-crystallin A chain (CRYAA) Small heat shock protein (HSP20) family P02489
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
X-box-binding protein 1 (XBP1) BZIP family P17861
Other
Click To Hide/Show 10 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
Cancer/testis antigen 1 (CTAG1A; CTAG1B) CTAG/PCC1 family P78358
Protein FAM168A (FAM168A) FAM168 family Q92567
Protein shisa-4 (SHISA4) Shisa family Q96DD7
Gelsolin (GSN) Villin/gelsolin family P06396
DAZ-associated protein 2 (DAZAP2) . Q15038
RING finger protein 11 (RNF11) . Q9Y3C5
TAR DNA-binding protein 43 (TARDBP) . Q13148
Ubiquilin-1 (UBQLN1) . Q9UMX0
Ubiquilin-2 (UBQLN2) . Q9UHD9

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
3 Ynamide Electrophile for the Profiling of Ligandable Carboxyl Residues in Live Cells and the Development of New Covalent Inhibitors. J Med Chem. 2022 Aug 11;65(15):10408-10418. doi: 10.1021/acs.jmedchem.2c00272. Epub 2022 Jul 26.
4 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
5 2H-Azirine-Based Reagents for Chemoselective Bioconjugation at Carboxyl Residues Inside Live Cells. J Am Chem Soc. 2020 Apr 1;142(13):6051-6059. doi: 10.1021/jacs.9b12116. Epub 2020 Mar 23.
6 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
7 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
8 Discovery of a Cell-Active SuTEx Ligand of Prostaglandin Reductase 2. Chembiochem. 2021 Jun 15;22(12):2134-2139. doi: 10.1002/cbic.202000879. Epub 2021 Apr 29.
9 Global targeting of functional tyrosines using sulfur-triazole exchange chemistry. Nat Chem Biol. 2020 Feb;16(2):150-159. doi: 10.1038/s41589-019-0404-5. Epub 2019 Nov 25.
10 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
11 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
12 Comparison of Quantitative Mass Spectrometry Platforms for Monitoring Kinase ATP Probe Uptake in Lung Cancer. J Proteome Res. 2018 Jan 5;17(1):63-75. doi: 10.1021/acs.jproteome.7b00329. Epub 2017 Nov 22.
Mass spectrometry data entry: PXD006095 , PXD006096
13 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
14 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
15 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
16 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
17 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
18 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
19 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
20 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
21 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
22 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
23 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.