Details of the Target

General Information of Target

Target ID LDTP03874
Target Name Homeobox protein cut-like 1 (CUX1)
Gene Name CUX1
Gene ID 1523
Synonyms
CUTL1; Homeobox protein cut-like 1; CCAAT displacement protein; CDP; CDP/Cux p200; Homeobox protein cux-1) [Cleaved into: CDP/Cux p110]
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLCVAGARLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSF
QGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRE
TLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQ
ETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERAN
QRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAIEVLTRSSLEVELAAKEREIA
QLVEDVQRLQASLTKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQADYEEVKKELNI
LKSMEFAPSEGAGTQDAAKPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPES
RRPGSLPAPPPSQLPRNPGEQASNTNGTHQFSPAGLSQDFFSSSLASPSLPLASTGKFAL
NSLLQRQLMQSFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSE
SAGSVSEGEEMDTAEIARQVKEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLT
VRGKEPFHKMKQFLSDEQNILALRSIQGRQRENPGQSLNRLFQEVPKRRNGSEGNITTRI
RASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASGSGNSDDAIRSILQQARREMEAQ
QAALDPALKQAPLSQSDITILTPKLLSTSPMPTVSSYPPLAISLKKPSAAPEAGASALPN
PPALKKEAQDAPGLDPQGAADCAQGVLRQVKNEVGRSGAWKDHWWSAVQPERRNAASSEE
AKAEETGGGKEKGSGGSGGGSQPRAERSQLQGPSSSEYWKEWPSAESPYSQSSELSLTGA
SRSETPQNSPLPSSPIVPMSKPTKPSVPPLTPEQYEVYMYQEVDTIELTRQVKEKLAKNG
ICQRIFGEKVLGLSQGSVSDMLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQ
QQGPVLHSVTSLQDPLQQGCVSSESTPKTSASCSPAPESPMSSSESVKSLTELVQQPCPP
IEASKDSKPPEPSDPPASDSQPTTPLPLSGHSALSIQELVAMSPELDTYGITKRVKEVLT
DNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLNDPNNVEKLMD
MKRMEKKAYMKRRHSSVSDSQPCEPPSVGTEYSQGASPQPQHQLKKPRVVLAPEEKEALK
RAYQQKPYPSPKTIEDLATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASD
SPSARSGRAAPSSEGDSCDGVEATEGPGSADTEEPKSQGEAEREEVPRPAEQTEPPPSGT
PGPDDARDDDHEGGPVEGPGPLPSPASATATAAPAAPEDAATSAAAAPGEGPAAPSSAPP
PSNSSSSSAPRRPSSLQSLFGLPEAAGARDSRDNPLRKKKAANLNSIIHRLEKAASREEP
IEWEF
Target Bioclass
Transcription factor
Family
CUT homeobox family
Subcellular location
Nucleus
Function
Transcription factor involved in the control of neuronal differentiation in the brain. Regulates dendrite development and branching, and dendritic spine formation in cortical layers II-III. Also involved in the control of synaptogenesis. In addition, it has probably a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator.; [CDP/Cux p110]: Plays a role in cell cycle progression, in particular at the G1/S transition. As cells progress into S phase, a fraction of CUX1 molecules is proteolytically processed into N-terminally truncated proteins of 110 kDa. While CUX1 only transiently binds to DNA and carries the CCAAT-displacement activity, CDP/Cux p110 makes a stable interaction with DNA and stimulates expression of genes such as POLA1.
Uniprot ID
P39880
Ensemble ID
ENST00000292535.12
HGNC ID
HGNC:2557

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
22RV1 Deletion: p.K344NfsTer11 .
A3KAW SNV: p.Q37H DBIA    Probe Info 
AGS SNV: p.S854C .
CCK81 SNV: p.K346N .
HCT116 SNV: p.Q869Ter .
HEC1 SNV: p.R1325H DBIA    Probe Info 
HEC1B SNV: p.R572H .
IGROV1 SNV: p.G855D .
IPC298 SNV: p.A1329V .
Ishikawa (Heraklio) 02 ER SNV: p.N80T .
JURKAT SNV: p.G967R .
KMCH1 SNV: p.N396I DBIA    Probe Info 
KMS12BM SNV: p.T314N .
L428 SNV: p.R421Q .
MCC26 SNV: p.P380L .
NCIH2286 SNV: p.R317L .
NUGC3 SNV: p.E877Ter .
P31FUJ SNV: p.A1415T .
RKO SNV: p.N44S; p.K140E .
SNU1 SNV: p.R1134W .
SUPT1 Deletion: p.Q1239SfsTer6 .
TFK1 SNV: p.A1412T .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 12 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
Probe 1
 Probe Info  		Y125(23.27)  LDD3495  [2]
DBIA
 Probe Info  		C421(2.78)  LDD3314  [3]
JZ128-DTB
 Probe Info  		N.A.  LDD0462  [4]
AHL-Pu-1
 Probe Info  		C802(5.60); C1078(2.80)  LDD0168  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [6]
IPM
 Probe Info  		N.A.  LDD0025  [7]
NAIA_5
 Probe Info  		C802(0.00); C1053(0.00)  LDD2224  [8]
TFBX
 Probe Info  		N.A.  LDD0027  [7]
VSF
 Probe Info  		C802(0.00); C1078(0.00)  LDD0007  [9]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [10]
AOyne
 Probe Info  		10.30  LDD0443  [11]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA HEK-293T C802(5.60); C1078(2.80)  LDD0168  [5]
 LDCM0026  4SU-RNA+native RNA HEK-293T C802(5.57); C1078(3.19)  LDD0169  [5]
 LDCM0215  AC10 HEK-293T C802(1.04)  LDD1508  [12]
 LDCM0237  AC12 HEK-293T C1078(1.10)  LDD1510  [12]
 LDCM0259  AC14 HEK-293T C802(0.84)  LDD1512  [12]
 LDCM0270  AC15 HEK-293T C802(1.21)  LDD1513  [12]
 LDCM0277  AC18 HEK-293T C802(0.77)  LDD1516  [12]
 LDCM0279  AC2 HEK-293T C802(1.39)  LDD1518  [12]
 LDCM0280  AC20 HEK-293T C1078(1.27)  LDD1519  [12]
 LDCM0281  AC21 HEK-293T C802(0.89); C1078(0.92)  LDD1520  [12]
 LDCM0282  AC22 HEK-293T C802(0.89)  LDD1521  [12]
 LDCM0283  AC23 HEK-293T C802(0.98)  LDD1522  [12]
 LDCM0284  AC24 HEK-293T C802(0.86)  LDD1523  [12]
 LDCM0286  AC26 HEK-293T C802(0.99)  LDD1525  [12]
 LDCM0288  AC28 HEK-293T C1078(0.93)  LDD1527  [12]
 LDCM0289  AC29 HEK-293T C802(1.01); C1078(0.94)  LDD1528  [12]
 LDCM0291  AC30 HEK-293T C802(1.11)  LDD1530  [12]
 LDCM0292  AC31 HEK-293T C802(0.79)  LDD1531  [12]
 LDCM0293  AC32 HEK-293T C802(0.88)  LDD1532  [12]
 LDCM0295  AC34 HEK-293T C802(1.09)  LDD1534  [12]
 LDCM0297  AC36 HEK-293T C1078(1.28)  LDD1536  [12]
 LDCM0298  AC37 HEK-293T C802(1.02); C1078(1.15)  LDD1537  [12]
 LDCM0299  AC38 HEK-293T C802(0.91)  LDD1538  [12]
 LDCM0300  AC39 HEK-293T C802(0.96)  LDD1539  [12]
 LDCM0301  AC4 HEK-293T C1078(1.06)  LDD1540  [12]
 LDCM0302  AC40 HEK-293T C802(1.13)  LDD1541  [12]
 LDCM0304  AC42 HEK-293T C802(1.36)  LDD1543  [12]
 LDCM0306  AC44 HEK-293T C1078(1.07)  LDD1545  [12]
 LDCM0307  AC45 HEK-293T C802(0.99); C1078(1.13)  LDD1546  [12]
 LDCM0308  AC46 HEK-293T C802(0.87)  LDD1547  [12]
 LDCM0309  AC47 HEK-293T C802(0.87)  LDD1548  [12]
 LDCM0310  AC48 HEK-293T C802(1.00)  LDD1549  [12]
 LDCM0312  AC5 HEK-293T C802(1.01); C1078(1.02)  LDD1551  [12]
 LDCM0313  AC50 HEK-293T C802(0.78)  LDD1552  [12]
 LDCM0315  AC52 HEK-293T C1078(1.03)  LDD1554  [12]
 LDCM0316  AC53 HEK-293T C802(0.90); C1078(1.08)  LDD1555  [12]
 LDCM0317  AC54 HEK-293T C802(1.06)  LDD1556  [12]
 LDCM0318  AC55 HEK-293T C802(0.71)  LDD1557  [12]
 LDCM0319  AC56 HEK-293T C802(1.07)  LDD1558  [12]
 LDCM0321  AC58 HEK-293T C802(0.88)  LDD1560  [12]
 LDCM0323  AC6 HEK-293T C802(1.11)  LDD1562  [12]
 LDCM0324  AC60 HEK-293T C1078(0.88)  LDD1563  [12]
 LDCM0325  AC61 HEK-293T C802(1.08); C1078(1.05)  LDD1564  [12]
 LDCM0326  AC62 HEK-293T C802(1.06)  LDD1565  [12]
 LDCM0327  AC63 HEK-293T C802(0.88)  LDD1566  [12]
 LDCM0328  AC64 HEK-293T C802(0.98)  LDD1567  [12]
 LDCM0334  AC7 HEK-293T C802(0.64)  LDD1568  [12]
 LDCM0345  AC8 HEK-293T C802(0.98)  LDD1569  [12]
 LDCM0248  AKOS034007472 HEK-293T C802(0.96); C1078(0.95)  LDD1511  [12]
 LDCM0275  AKOS034007705 HEK-293T C802(1.10)  LDD1514  [12]
 LDCM0156  Aniline NCI-H1299 12.68  LDD0403  [1]
 LDCM0632  CL-Sc Hep-G2 C1223(20.00)  LDD2227  [8]
 LDCM0367  CL1 HEK-293T C802(1.03)  LDD1571  [12]
 LDCM0368  CL10 HEK-293T C802(0.87)  LDD1572  [12]
 LDCM0370  CL101 HEK-293T C802(1.07)  LDD1574  [12]
 LDCM0371  CL102 HEK-293T C802(1.08); C1078(1.02)  LDD1575  [12]
 LDCM0374  CL105 HEK-293T C802(1.11)  LDD1578  [12]
 LDCM0375  CL106 HEK-293T C802(0.78); C1078(0.96)  LDD1579  [12]
 LDCM0378  CL109 HEK-293T C802(0.93)  LDD1582  [12]
 LDCM0379  CL11 HEK-293T C802(0.89)  LDD1583  [12]
 LDCM0380  CL110 HEK-293T C802(1.06); C1078(1.01)  LDD1584  [12]
 LDCM0383  CL113 HEK-293T C802(1.14)  LDD1587  [12]
 LDCM0384  CL114 HEK-293T C802(1.00); C1078(0.90)  LDD1588  [12]
 LDCM0387  CL117 HEK-293T C802(0.94)  LDD1591  [12]
 LDCM0388  CL118 HEK-293T C802(0.98); C1078(1.18)  LDD1592  [12]
 LDCM0390  CL12 HEK-293T C802(1.05)  LDD1594  [12]
 LDCM0392  CL121 HEK-293T C802(1.03)  LDD1596  [12]
 LDCM0393  CL122 HEK-293T C802(1.10); C1078(1.07)  LDD1597  [12]
 LDCM0396  CL125 HEK-293T C802(1.02)  LDD1600  [12]
 LDCM0397  CL126 HEK-293T C802(1.05); C1078(1.10)  LDD1601  [12]
 LDCM0400  CL13 HEK-293T C802(1.04)  LDD1604  [12]
 LDCM0401  CL14 HEK-293T C802(1.13); C1078(1.11)  LDD1605  [12]
 LDCM0405  CL18 HEK-293T C802(1.17)  LDD1609  [12]
 LDCM0407  CL2 HEK-293T C802(1.02); C1078(1.21)  LDD1611  [12]
 LDCM0408  CL20 HEK-293T C1078(1.00)  LDD1612  [12]
 LDCM0409  CL21 HEK-293T C802(1.08); C1078(1.04)  LDD1613  [12]
 LDCM0410  CL22 HEK-293T C802(1.01)  LDD1614  [12]
 LDCM0411  CL23 HEK-293T C802(0.78)  LDD1615  [12]
 LDCM0412  CL24 HEK-293T C802(1.09)  LDD1616  [12]
 LDCM0413  CL25 HEK-293T C802(0.88)  LDD1617  [12]
 LDCM0414  CL26 HEK-293T C802(0.94); C1078(1.24)  LDD1618  [12]
 LDCM0419  CL30 HEK-293T C802(1.07)  LDD1623  [12]
 LDCM0421  CL32 HEK-293T C1078(1.02)  LDD1625  [12]
 LDCM0422  CL33 HEK-293T C802(0.87); C1078(0.93)  LDD1626  [12]
 LDCM0423  CL34 HEK-293T C802(0.92)  LDD1627  [12]
 LDCM0424  CL35 HEK-293T C802(0.79)  LDD1628  [12]
 LDCM0425  CL36 HEK-293T C802(1.11)  LDD1629  [12]
 LDCM0426  CL37 HEK-293T C802(0.98)  LDD1630  [12]
 LDCM0432  CL42 HEK-293T C802(0.85)  LDD1636  [12]
 LDCM0434  CL44 HEK-293T C1078(1.10)  LDD1638  [12]
 LDCM0435  CL45 HEK-293T C802(1.09); C1078(1.01)  LDD1639  [12]
 LDCM0436  CL46 HEK-293T C802(0.95)  LDD1640  [12]
 LDCM0437  CL47 HEK-293T C802(0.88)  LDD1641  [12]
 LDCM0438  CL48 HEK-293T C802(1.05)  LDD1642  [12]
 LDCM0439  CL49 HEK-293T C802(0.84)  LDD1643  [12]
 LDCM0441  CL50 HEK-293T C802(0.79); C1078(1.38)  LDD1645  [12]
 LDCM0445  CL54 HEK-293T C802(0.78)  LDD1648  [12]
 LDCM0447  CL56 HEK-293T C1078(1.09)  LDD1650  [12]
 LDCM0448  CL57 HEK-293T C802(0.85); C1078(1.20)  LDD1651  [12]
 LDCM0449  CL58 HEK-293T C802(0.99)  LDD1652  [12]
 LDCM0450  CL59 HEK-293T C802(0.90)  LDD1653  [12]
 LDCM0451  CL6 HEK-293T C802(1.07)  LDD1654  [12]
 LDCM0452  CL60 HEK-293T C802(1.04)  LDD1655  [12]
 LDCM0453  CL61 HEK-293T C802(1.00)  LDD1656  [12]
 LDCM0454  CL62 HEK-293T C802(1.15); C1078(1.12)  LDD1657  [12]
 LDCM0458  CL66 HEK-293T C802(1.03)  LDD1661  [12]
 LDCM0460  CL68 HEK-293T C1078(1.22)  LDD1663  [12]
 LDCM0461  CL69 HEK-293T C802(0.88); C1078(1.15)  LDD1664  [12]
 LDCM0463  CL70 HEK-293T C802(1.07)  LDD1666  [12]
 LDCM0464  CL71 HEK-293T C802(0.90)  LDD1667  [12]
 LDCM0465  CL72 HEK-293T C802(1.17)  LDD1668  [12]
 LDCM0466  CL73 HEK-293T C802(0.90)  LDD1669  [12]
 LDCM0467  CL74 HEK-293T C802(0.97); C1078(1.05)  LDD1670  [12]
 LDCM0471  CL78 HEK-293T C802(0.88)  LDD1674  [12]
 LDCM0473  CL8 HEK-293T C1078(1.16)  LDD1676  [12]
 LDCM0474  CL80 HEK-293T C1078(0.97)  LDD1677  [12]
 LDCM0475  CL81 HEK-293T C802(1.11); C1078(1.05)  LDD1678  [12]
 LDCM0476  CL82 HEK-293T C802(1.11)  LDD1679  [12]
 LDCM0477  CL83 HEK-293T C802(1.00)  LDD1680  [12]
 LDCM0478  CL84 HEK-293T C802(0.99)  LDD1681  [12]
 LDCM0479  CL85 HEK-293T C802(0.93)  LDD1682  [12]
 LDCM0480  CL86 HEK-293T C802(0.78); C1078(1.31)  LDD1683  [12]
 LDCM0484  CL9 HEK-293T C802(1.08); C1078(0.98)  LDD1687  [12]
 LDCM0485  CL90 HEK-293T C802(0.70)  LDD1688  [12]
 LDCM0487  CL92 HEK-293T C1078(0.91)  LDD1690  [12]
 LDCM0488  CL93 HEK-293T C802(1.04); C1078(1.19)  LDD1691  [12]
 LDCM0489  CL94 HEK-293T C802(1.05)  LDD1692  [12]
 LDCM0490  CL95 HEK-293T C802(0.67)  LDD1693  [12]
 LDCM0491  CL96 HEK-293T C802(0.91)  LDD1694  [12]
 LDCM0492  CL97 HEK-293T C802(0.87)  LDD1695  [12]
 LDCM0493  CL98 HEK-293T C802(1.00); C1078(1.13)  LDD1696  [12]
 LDCM0634  CY-0357 Hep-G2 C1338(0.74)  LDD2228  [8]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C962(7.55)  LDD1702  [13]
 LDCM0427  Fragment51 HEK-293T C802(0.83); C1078(1.11)  LDD1631  [12]
 LDCM0179  JZ128 PC-3 N.A.  LDD0462  [4]
 LDCM0022  KB02 HEK-293T C1338(0.90); C802(1.06); C1078(1.10)  LDD1492  [12]
 LDCM0023  KB03 HEK-293T C1338(0.94); C802(1.02); C1078(1.22)  LDD1497  [12]
 LDCM0024  KB05 IGR37 C421(2.78)  LDD3314  [3]

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
4 A Chemoproteomic Strategy for Direct and Proteome-Wide Covalent Inhibitor Target-Site Identification. J Am Chem Soc. 2019 Jan 9;141(1):191-203. doi: 10.1021/jacs.8b07911. Epub 2018 Dec 20.
5 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
9 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
10 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
11 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
12 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
13 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761