Details of the Target

General Information of Target

Target ID LDTP03513
Target Name D-dopachrome decarboxylase (DDT)
Gene Name DDT
Gene ID 1652
Synonyms
D-dopachrome decarboxylase; EC 4.1.1.84; D-dopachrome tautomerase; Phenylpyruvate tautomerase II
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MPFLELDTNLPANRVPAGLEKRLCAAAASILGKPADRVNVTVRPGLAMALSGSTEPCAQL
SISSIGVVGTAEDNRSHSAHFFEFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL
Target Bioclass
Enzyme
Family
MIF family
Subcellular location
Cytoplasm
Function Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI).
Uniprot ID
P30046
Ensemble ID
ENST00000350608.7
HGNC ID
HGNC:2732

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
PC9 SNV: p.Q107K .
SNGM Deletion: p.L118YfsTer12 .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 14 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C24(1.33)  LDD2089  [1]
NAIA_5
 Probe Info  		C24(0.54)  LDD2227  [2]
5E-2FA
 Probe Info  		N.A.  LDD2235  [3]
m-APA
 Probe Info  		N.A.  LDD2231  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [4]
IPIAA_L
 Probe Info  		N.A.  LDD0031  [5]
IPM
 Probe Info  		N.A.  LDD0025  [6]
JW-RF-010
 Probe Info  		N.A.  LDD0026  [6]
SF
 Probe Info  		K21(0.00); K33(0.00)  LDD0028  [7]
STPyne
 Probe Info  		N.A.  LDD0009  [8]
TFBX
 Probe Info  		N.A.  LDD0148  [6]
1d-yne
 Probe Info  		N.A.  LDD0357  [9]
W1
 Probe Info  		C24(0.00); E5(0.00)  LDD0236  [10]
HHS-465
 Probe Info  		N.A.  LDD2240  [11]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C158
 Probe Info  		30.70  LDD1838  [12]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C24(0.31)  LDD2142  [1]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C24(0.41)  LDD2112  [1]
 LDCM0502  1-(Cyanoacetyl)piperidine MDA-MB-231 C24(0.69)  LDD2095  [1]
 LDCM0537  2-Cyano-N,N-dimethylacetamide MDA-MB-231 C24(0.66)  LDD2130  [1]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C24(0.92)  LDD2117  [1]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C24(1.44)  LDD2152  [1]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C24(0.47)  LDD2132  [1]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C24(0.68)  LDD2131  [1]
 LDCM0545  Acetamide MDA-MB-231 C24(0.79)  LDD2138  [1]
 LDCM0520  AKOS000195272 MDA-MB-231 C24(0.90)  LDD2113  [1]
 LDCM0498  BS-3668 MDA-MB-231 C24(0.85)  LDD2091  [1]
 LDCM0632  CL-Sc Hep-G2 C24(0.54)  LDD2227  [2]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C24(1.02)  LDD1702  [1]
 LDCM0625  F8 Ramos C24(0.72)  LDD2187  [13]
 LDCM0572  Fragment10 Ramos C24(0.78)  LDD2189  [13]
 LDCM0573  Fragment11 Ramos C24(0.04)  LDD2190  [13]
 LDCM0574  Fragment12 Ramos C24(0.80)  LDD2191  [13]
 LDCM0575  Fragment13 Ramos C24(1.16)  LDD2192  [13]
 LDCM0576  Fragment14 Ramos C24(0.72)  LDD2193  [13]
 LDCM0579  Fragment20 Ramos C24(0.93)  LDD2194  [13]
 LDCM0580  Fragment21 Ramos C24(1.07)  LDD2195  [13]
 LDCM0582  Fragment23 Ramos C24(1.62)  LDD2196  [13]
 LDCM0578  Fragment27 Ramos C24(1.18)  LDD2197  [13]
 LDCM0586  Fragment28 Ramos C24(0.93)  LDD2198  [13]
 LDCM0588  Fragment30 Ramos C24(1.06)  LDD2199  [13]
 LDCM0589  Fragment31 Ramos C24(1.68)  LDD2200  [13]
 LDCM0590  Fragment32 Ramos C24(0.67)  LDD2201  [13]
 LDCM0468  Fragment33 Ramos C24(0.91)  LDD2202  [13]
 LDCM0596  Fragment38 Ramos C24(0.86)  LDD2203  [13]
 LDCM0566  Fragment4 Ramos C24(0.69)  LDD2184  [13]
 LDCM0610  Fragment52 Ramos C24(1.13)  LDD2204  [13]
 LDCM0614  Fragment56 Ramos C24(1.02)  LDD2205  [13]
 LDCM0569  Fragment7 Ramos C24(0.47)  LDD2186  [13]
 LDCM0571  Fragment9 Ramos C24(0.82)  LDD2188  [13]
 LDCM0022  KB02 Ramos C24(0.93)  LDD2182  [13]
 LDCM0023  KB03 MDA-MB-231 C24(1.61)  LDD1701  [1]
 LDCM0024  KB05 Ramos C24(0.45)  LDD2185  [13]
 LDCM0509  N-(4-bromo-3,5-dimethylphenyl)-2-nitroacetamide MDA-MB-231 C24(1.00)  LDD2102  [1]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C24(1.33)  LDD2089  [1]
 LDCM0497  Nucleophilic fragment 11b MDA-MB-231 C24(0.93)  LDD2090  [1]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C24(1.26)  LDD2092  [1]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C24(1.34)  LDD2093  [1]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C24(0.32)  LDD2096  [1]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C24(0.82)  LDD2097  [1]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C24(0.82)  LDD2098  [1]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C24(1.32)  LDD2099  [1]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C24(0.45)  LDD2104  [1]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C24(1.94)  LDD2105  [1]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C24(0.81)  LDD2107  [1]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C24(0.60)  LDD2109  [1]
 LDCM0517  Nucleophilic fragment 21b MDA-MB-231 C24(0.51)  LDD2110  [1]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C24(1.09)  LDD2111  [1]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C24(0.36)  LDD2114  [1]
 LDCM0523  Nucleophilic fragment 24b MDA-MB-231 C24(0.64)  LDD2116  [1]
 LDCM0525  Nucleophilic fragment 25b MDA-MB-231 C24(0.77)  LDD2118  [1]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C24(1.94)  LDD2119  [1]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C24(0.62)  LDD2120  [1]
 LDCM0529  Nucleophilic fragment 27b MDA-MB-231 C24(0.61)  LDD2122  [1]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C24(0.85)  LDD2123  [1]
 LDCM0531  Nucleophilic fragment 28b MDA-MB-231 C24(0.60)  LDD2124  [1]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C24(0.88)  LDD2125  [1]
 LDCM0533  Nucleophilic fragment 29b MDA-MB-231 C24(0.60)  LDD2126  [1]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C24(1.00)  LDD2127  [1]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C24(0.65)  LDD2128  [1]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C24(1.30)  LDD2129  [1]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C24(0.44)  LDD2133  [1]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C24(0.39)  LDD2134  [1]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C24(1.53)  LDD2135  [1]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C24(1.39)  LDD2136  [1]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C24(0.87)  LDD2137  [1]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C24(0.77)  LDD2140  [1]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C24(0.29)  LDD2141  [1]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C24(0.47)  LDD2143  [1]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C24(1.97)  LDD2144  [1]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C24(0.85)  LDD2146  [1]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C24(1.22)  LDD2147  [1]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C24(0.40)  LDD2148  [1]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C24(0.89)  LDD2150  [1]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C24(0.27)  LDD2151  [1]

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 SP3-Enabled Rapid and High Coverage Chemoproteomic Identification of Cell-State-Dependent Redox-Sensitive Cysteines. Mol Cell Proteomics. 2022 Apr;21(4):100218. doi: 10.1016/j.mcpro.2022.100218. Epub 2022 Feb 25.
Mass spectrometry data entry: PXD029500 , PXD031647
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 Solid Phase Synthesis of Fluorosulfate Containing Macrocycles for Chemoproteomic Workflows. bioRxiv [Preprint]. 2023 Feb 18:2023.02.17.529022. doi: 10.1101/2023.02.17.529022.
Mass spectrometry data entry: PXD039931
8 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
9 Tunable Amine-Reactive Electrophiles for Selective Profiling of Lysine. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202112107. doi: 10.1002/anie.202112107. Epub 2021 Dec 16.
10 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
11 Global profiling identifies a stress-responsive tyrosine site on EDC3 regulating biomolecular condensate formation. Cell Chem Biol. 2022 Dec 15;29(12):1709-1720.e7. doi: 10.1016/j.chembiol.2022.11.008. Epub 2022 Dec 6.
Mass spectrometry data entry: PXD038010
12 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
13 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578