Details of the Target

General Information of Target

Target ID LDTP02777
Target Name Beta-1,4-galactosyltransferase 1 (B4GALT1)
Gene Name B4GALT1
Gene ID 2683
Synonyms
GGTB2; Beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1; EC 2.4.1.-; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; EC 2.4.1.38; Lactose synthase A protein; EC 2.4.1.22; N-acetyllactosamine synthase; EC 2.4.1.90; Nal synthase; Neolactotriaosylceramide beta-1,4-galactosyltransferase; EC 2.4.1.275; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1) [Cleaved into: Processed beta-1,4-galactosyltransferase 1]
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MRLREPLLSGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGRDLSRLPQLVGVSTPL
QGGSNSAAAIGQSSGELRTGGARPPPPLGASSQPRPGGDSSPVVDSGPGPASNLTSVPVP
HTTALSLPACPEESPLLVGPMLIEFNMPVDLELVAKQNPNVKMGGRYAPRDCVSPHKVAI
IIPFRNRQEHLKYWLYYLHPVLQRQQLDYGIYVINQAGDTIFNRAKLLNVGFQEALKDYD
YTCFVFSDVDLIPMNDHNAYRCFSQPRHISVAMDKFGFSLPYVQYFGGVSALSKQQFLTI
NGFPNNYWGWGGEDDDIFNRLVFRGMSISRPNAVVGRCRMIRHSRDKKNEPNPQRFDRIA
HTKETMLSDGLNSLTYQVLDVQRYPLYTQITVDIGTPS
Target Bioclass
Enzyme
Family
Glycosyltransferase 7 family
Subcellular location
Secreted; Golgi apparatus, Golgi stack membrane
Function
[Beta-1,4-galactosyltransferase 1]: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.; [Processed beta-1,4-galactosyltransferase 1]: The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
Uniprot ID
P15291
Ensemble ID
ENST00000379731.5
HGNC ID
HGNC:924
ChEMBL ID
CHEMBL4384

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HCC15 SNV: p.Y196C .
HT115 SNV: p.R342H .
MFE296 SNV: p.V36I .
MFE319 SNV: p.I70T; p.N158S .
NCIH2286 SNV: p.S134C .
SHP77 SNV: p.R95C .
SW837 Substitution: p.S264Q .
TE1 SNV: p.S134A .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 8 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C172(1.76)  LDD1699  [1]
DBIA
 Probe Info  		C172(2.22)  LDD3320  [2]
Acrolein
 Probe Info  		N.A.  LDD0223  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [4]
TFBX
 Probe Info  		N.A.  LDD0027  [5]
Crotonaldehyde
 Probe Info  		N.A.  LDD0219  [3]
Methacrolein
 Probe Info  		N.A.  LDD0218  [3]
NAIA_5
 Probe Info  		N.A.  LDD2223  [6]
PAL-AfBPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C056
 Probe Info  		16.91  LDD1753  [7]
STS-1
 Probe Info  		N.A.  LDD0136  [8]
STS-2
 Probe Info  		1.35  LDD0138  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C172(0.87)  LDD2117  [1]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C172(0.88)  LDD2152  [1]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C172(0.93)  LDD2132  [1]
 LDCM0538  4-(Cyanoacetyl)morpholine MDA-MB-231 C172(0.92)  LDD2131  [1]
 LDCM0215  AC10 HEK-293T C172(0.94)  LDD1508  [9]
 LDCM0259  AC14 HEK-293T C172(1.00)  LDD1512  [9]
 LDCM0277  AC18 HEK-293T C172(1.07)  LDD1516  [9]
 LDCM0279  AC2 HEK-293T C172(0.79)  LDD1518  [9]
 LDCM0282  AC22 HEK-293T C172(1.29)  LDD1521  [9]
 LDCM0284  AC24 HEK-293T C172(0.94)  LDD1523  [9]
 LDCM0286  AC26 HEK-293T C172(0.94)  LDD1525  [9]
 LDCM0291  AC30 HEK-293T C172(1.24)  LDD1530  [9]
 LDCM0293  AC32 HEK-293T C172(1.15)  LDD1532  [9]
 LDCM0295  AC34 HEK-293T C172(1.01)  LDD1534  [9]
 LDCM0299  AC38 HEK-293T C172(1.17)  LDD1538  [9]
 LDCM0302  AC40 HEK-293T C172(1.15)  LDD1541  [9]
 LDCM0304  AC42 HEK-293T C172(1.07)  LDD1543  [9]
 LDCM0308  AC46 HEK-293T C172(1.50)  LDD1547  [9]
 LDCM0310  AC48 HEK-293T C172(1.33)  LDD1549  [9]
 LDCM0313  AC50 HEK-293T C172(0.97)  LDD1552  [9]
 LDCM0317  AC54 HEK-293T C172(1.31)  LDD1556  [9]
 LDCM0319  AC56 HEK-293T C172(1.18)  LDD1558  [9]
 LDCM0321  AC58 HEK-293T C172(0.90)  LDD1560  [9]
 LDCM0323  AC6 HEK-293T C172(1.22)  LDD1562  [9]
 LDCM0326  AC62 HEK-293T C172(0.92)  LDD1565  [9]
 LDCM0328  AC64 HEK-293T C172(0.93)  LDD1567  [9]
 LDCM0345  AC8 HEK-293T C172(0.98)  LDD1569  [9]
 LDCM0545  Acetamide MDA-MB-231 C172(0.54)  LDD2138  [1]
 LDCM0520  AKOS000195272 MDA-MB-231 C172(0.99)  LDD2113  [1]
 LDCM0275  AKOS034007705 HEK-293T C172(0.91)  LDD1514  [9]
 LDCM0368  CL10 HEK-293T C172(1.16)  LDD1572  [9]
 LDCM0369  CL100 HEK-293T C172(0.62)  LDD1573  [9]
 LDCM0373  CL104 HEK-293T C172(0.91)  LDD1577  [9]
 LDCM0377  CL108 HEK-293T C172(0.89)  LDD1581  [9]
 LDCM0382  CL112 HEK-293T C172(0.99)  LDD1586  [9]
 LDCM0386  CL116 HEK-293T C172(1.01)  LDD1590  [9]
 LDCM0390  CL12 HEK-293T C172(1.03)  LDD1594  [9]
 LDCM0391  CL120 HEK-293T C172(0.99)  LDD1595  [9]
 LDCM0395  CL124 HEK-293T C172(0.90)  LDD1599  [9]
 LDCM0399  CL128 HEK-293T C172(0.87)  LDD1603  [9]
 LDCM0403  CL16 HEK-293T C172(0.93)  LDD1607  [9]
 LDCM0405  CL18 HEK-293T C172(0.97)  LDD1609  [9]
 LDCM0410  CL22 HEK-293T C172(1.35)  LDD1614  [9]
 LDCM0412  CL24 HEK-293T C172(0.92)  LDD1616  [9]
 LDCM0416  CL28 HEK-293T C172(0.95)  LDD1620  [9]
 LDCM0419  CL30 HEK-293T C172(0.95)  LDD1623  [9]
 LDCM0423  CL34 HEK-293T C172(1.30)  LDD1627  [9]
 LDCM0425  CL36 HEK-293T C172(0.85)  LDD1629  [9]
 LDCM0429  CL4 HEK-293T C172(0.88)  LDD1633  [9]
 LDCM0430  CL40 HEK-293T C172(0.96)  LDD1634  [9]
 LDCM0432  CL42 HEK-293T C172(0.83)  LDD1636  [9]
 LDCM0436  CL46 HEK-293T C172(1.01)  LDD1640  [9]
 LDCM0438  CL48 HEK-293T C172(1.05)  LDD1642  [9]
 LDCM0443  CL52 HEK-293T C172(0.97)  LDD1646  [9]
 LDCM0445  CL54 HEK-293T C172(1.12)  LDD1648  [9]
 LDCM0449  CL58 HEK-293T C172(1.16)  LDD1652  [9]
 LDCM0451  CL6 HEK-293T C172(1.10)  LDD1654  [9]
 LDCM0452  CL60 HEK-293T C172(1.00)  LDD1655  [9]
 LDCM0456  CL64 HEK-293T C172(1.15)  LDD1659  [9]
 LDCM0458  CL66 HEK-293T C172(1.04)  LDD1661  [9]
 LDCM0463  CL70 HEK-293T C172(1.10)  LDD1666  [9]
 LDCM0465  CL72 HEK-293T C172(1.00)  LDD1668  [9]
 LDCM0469  CL76 HEK-293T C172(0.98)  LDD1672  [9]
 LDCM0471  CL78 HEK-293T C172(0.97)  LDD1674  [9]
 LDCM0476  CL82 HEK-293T C172(1.01)  LDD1679  [9]
 LDCM0478  CL84 HEK-293T C172(0.94)  LDD1681  [9]
 LDCM0482  CL88 HEK-293T C172(0.77)  LDD1685  [9]
 LDCM0485  CL90 HEK-293T C172(1.02)  LDD1688  [9]
 LDCM0489  CL94 HEK-293T C172(1.14)  LDD1692  [9]
 LDCM0491  CL96 HEK-293T C172(1.12)  LDD1694  [9]
 LDCM0022  KB02 786-O C172(1.86)  LDD2247  [2]
 LDCM0023  KB03 786-O C172(2.71)  LDD2664  [2]
 LDCM0024  KB05 RVH421 C172(2.22)  LDD3320  [2]
 LDCM0109  NEM HeLa N.A.  LDD0223  [3]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C172(0.38)  LDD2089  [1]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C172(0.80)  LDD2093  [1]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C172(0.74)  LDD2099  [1]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C172(0.53)  LDD2100  [1]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C172(1.66)  LDD2101  [1]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C172(0.68)  LDD2104  [1]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C172(0.69)  LDD2107  [1]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C172(1.04)  LDD2109  [1]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C172(1.04)  LDD2111  [1]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C172(1.06)  LDD2115  [1]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C172(1.26)  LDD2119  [1]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C172(0.74)  LDD2123  [1]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C172(0.98)  LDD2125  [1]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C172(0.80)  LDD2127  [1]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C172(0.84)  LDD2128  [1]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C172(0.98)  LDD2129  [1]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C172(1.25)  LDD2133  [1]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C172(0.78)  LDD2134  [1]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C172(0.93)  LDD2135  [1]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C172(0.99)  LDD2136  [1]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C172(0.98)  LDD2137  [1]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C172(1.15)  LDD1700  [1]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C172(0.99)  LDD2140  [1]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C172(1.10)  LDD2141  [1]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C172(0.63)  LDD2144  [1]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C172(0.80)  LDD2146  [1]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C172(0.97)  LDD2148  [1]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
N-acetylglucosamine Small molecular drug DB00141
Investigative
Click To Hide/Show 5 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
2-(N-morpholino)Ethanesulfonic Acid Small molecular drug DB03814
Galactose-uridine-5'-diphosphate Small molecular drug DB03501
Uridine Monophosphate Small molecular drug DB03685
6-aminohexyl-uridine-c15'-diphosphate . DB02696
N-acetyl-beta-d-glucosaminyl-(1->4)-n-acetyl-beta-d-glucosamine . DB03013

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Design and synthesis of minimalist terminal alkyne-containing diazirine photo-crosslinkers and their incorporation into kinase inhibitors for cell- and tissue-based proteome profiling. Angew Chem Int Ed Engl. 2013 Aug 12;52(33):8551-6. doi: 10.1002/anie.201300683. Epub 2013 Jun 10.
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402