Details of the Target

General Information of Target

Target ID LDTP02282
Target Name Collagen alpha-2(IV) chain (COL4A2)
Gene Name COL4A2
Gene ID 1284
Synonyms
Collagen alpha-2(IV) chain [Cleaved into: Canstatin]
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MGRDQRAVAGPALRRWLLLGTVTVGFLAQSVLAGVKKFDVPCGGRDCSGGCQCYPEKGGR
GQPGPVGPQGYNGPPGLQGFPGLQGRKGDKGERGAPGVTGPKGDVGARGVSGFPGADGIP
GHPGQGGPRGRPGYDGCNGTQGDSGPQGPPGSEGFTGPPGPQGPKGQKGEPYALPKEERD
RYRGEPGEPGLVGFQGPPGRPGHVGQMGPVGAPGRPGPPGPPGPKGQQGNRGLGFYGVKG
EKGDVGQPGPNGIPSDTLHPIIAPTGVTFHPDQYKGEKGSEGEPGIRGISLKGEEGIMGF
PGLRGYPGLSGEKGSPGQKGSRGLDGYQGPDGPRGPKGEAGDPGPPGLPAYSPHPSLAKG
ARGDPGFPGAQGEPGSQGEPGDPGLPGPPGLSIGDGDQRRGLPGEMGPKGFIGDPGIPAL
YGGPPGPDGKRGPPGPPGLPGPPGPDGFLFGLKGAKGRAGFPGLPGSPGARGPKGWKGDA
GECRCTEGDEAIKGLPGLPGPKGFAGINGEPGRKGDRGDPGQHGLPGFPGLKGVPGNIGA
PGPKGAKGDSRTITTKGERGQPGVPGVPGMKGDDGSPGRDGLDGFPGLPGPPGDGIKGPP
GDPGYPGIPGTKGTPGEMGPPGLGLPGLKGQRGFPGDAGLPGPPGFLGPPGPAGTPGQID
CDTDVKRAVGGDRQEAIQPGCIGGPKGLPGLPGPPGPTGAKGLRGIPGFAGADGGPGPRG
LPGDAGREGFPGPPGFIGPRGSKGAVGLPGPDGSPGPIGLPGPDGPPGERGLPGEVLGAQ
PGPRGDAGVPGQPGLKGLPGDRGPPGFRGSQGMPGMPGLKGQPGLPGPSGQPGLYGPPGL
HGFPGAPGQEGPLGLPGIPGREGLPGDRGDPGDTGAPGPVGMKGLSGDRGDAGFTGEQGH
PGSPGFKGIDGMPGTPGLKGDRGSPGMDGFQGMPGLKGRPGFPGSKGEAGFFGIPGLKGL
AGEPGFKGSRGDPGPPGPPPVILPGMKDIKGEKGDEGPMGLKGYLGAKGIQGMPGIPGLS
GIPGLPGRPGHIKGVKGDIGVPGIPGLPGFPGVAGPPGITGFPGFIGSRGDKGAPGRAGL
YGEIGATGDFGDIGDTINLPGRPGLKGERGTTGIPGLKGFFGEKGTEGDIGFPGITGVTG
VQGPPGLKGQTGFPGLTGPPGSQGELGRIGLPGGKGDDGWPGAPGLPGFPGLRGIRGLHG
LPGTKGFPGSPGSDIHGDPGFPGPPGERGDPGEANTLPGPVGVPGQKGDQGAPGERGPPG
SPGLQGFPGITPPSNISGAPGDKGAPGIFGLKGYRGPPGPPGSAALPGSKGDTGNPGAPG
TPGTKGWAGDSGPQGRPGVFGLPGEKGPRGEQGFMGNTGPTGAVGDRGPKGPKGDPGFPG
APGTVGAPGIAGIPQKIAVQPGTVGPQGRRGPPGAPGEMGPQGPPGEPGFRGAPGKAGPQ
GRGGVSAVPGFRGDEGPIGHQGPIGQEGAPGRPGSPGLPGMPGRSVSIGYLLVKHSQTDQ
EPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASR
NDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLW
IGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIP
EQSFQGSPSADTLKAGLIRTHISRCQVCMKNL
Target Bioclass
Other
Family
Type IV collagen family
Subcellular location
Secreted, extracellular space, extracellular matrix, basement membrane
Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.
Uniprot ID
P08572
Ensemble ID
ENST00000360467.7
HGNC ID
HGNC:2203
ChEMBL ID
CHEMBL2364188

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DA-P2
 Probe Info  		8.20  LDD0348  [1]
HRP
 Probe Info  		4.19  LDD0347  [1]
DBIA
 Probe Info  		C1537(1.39); C1658(1.45)  LDD3316  [2]
BTD
 Probe Info  		C1665(1.42)  LDD2097  [3]
IA-alkyne
 Probe Info  		C1537(0.00); C1658(0.00); C1665(0.00); C42(0.00)  LDD0165  [4]
NAIA_5
 Probe Info  		N.A.  LDD2224  [5]
TFBX
 Probe Info  		N.A.  LDD0027  [6]
IPM
 Probe Info  		N.A.  LDD0147  [6]
STPyne
 Probe Info  		N.A.  LDD0009  [7]
Methacrolein
 Probe Info  		N.A.  LDD0218  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C42(1.19)  LDD1507  [9]
 LDCM0226  AC11 HEK-293T C42(1.04)  LDD1509  [9]
 LDCM0270  AC15 HEK-293T C42(1.14)  LDD1513  [9]
 LDCM0276  AC17 HEK-293T C42(1.04)  LDD1515  [9]
 LDCM0278  AC19 HEK-293T C42(3.00)  LDD1517  [9]
 LDCM0283  AC23 HEK-293T C42(0.96)  LDD1522  [9]
 LDCM0284  AC24 HEK-293T C42(0.99)  LDD1523  [9]
 LDCM0285  AC25 HEK-293T C42(1.12)  LDD1524  [9]
 LDCM0287  AC27 HEK-293T C42(1.06)  LDD1526  [9]
 LDCM0290  AC3 HEK-293T C42(1.01)  LDD1529  [9]
 LDCM0292  AC31 HEK-293T C42(0.98)  LDD1531  [9]
 LDCM0293  AC32 HEK-293T C42(1.00)  LDD1532  [9]
 LDCM0294  AC33 HEK-293T C42(1.30)  LDD1533  [9]
 LDCM0296  AC35 HEK-293T C42(1.05)  LDD1535  [9]
 LDCM0300  AC39 HEK-293T C42(1.09)  LDD1539  [9]
 LDCM0302  AC40 HEK-293T C42(1.02)  LDD1541  [9]
 LDCM0303  AC41 HEK-293T C42(1.17)  LDD1542  [9]
 LDCM0305  AC43 HEK-293T C42(1.06)  LDD1544  [9]
 LDCM0309  AC47 HEK-293T C42(1.07)  LDD1548  [9]
 LDCM0310  AC48 HEK-293T C42(1.11)  LDD1549  [9]
 LDCM0311  AC49 HEK-293T C42(1.12)  LDD1550  [9]
 LDCM0314  AC51 HEK-293T C42(1.04)  LDD1553  [9]
 LDCM0318  AC55 HEK-293T C42(1.04)  LDD1557  [9]
 LDCM0319  AC56 HEK-293T C42(1.11)  LDD1558  [9]
 LDCM0320  AC57 HEK-293T C42(1.17)  LDD1559  [9]
 LDCM0322  AC59 HEK-293T C42(1.13)  LDD1561  [9]
 LDCM0327  AC63 HEK-293T C42(1.06)  LDD1566  [9]
 LDCM0328  AC64 HEK-293T C42(1.02)  LDD1567  [9]
 LDCM0334  AC7 HEK-293T C42(1.03)  LDD1568  [9]
 LDCM0345  AC8 HEK-293T C42(1.19)  LDD1569  [9]
 LDCM0356  AKOS034007680 HEK-293T C42(1.29)  LDD1570  [9]
 LDCM0275  AKOS034007705 HEK-293T C42(1.05)  LDD1514  [9]
 LDCM0367  CL1 HEK-293T C42(0.98)  LDD1571  [9]
 LDCM0370  CL101 HEK-293T C42(1.06)  LDD1574  [9]
 LDCM0372  CL103 HEK-293T C42(1.03)  LDD1576  [9]
 LDCM0374  CL105 HEK-293T C42(1.04)  LDD1578  [9]
 LDCM0376  CL107 HEK-293T C42(1.00)  LDD1580  [9]
 LDCM0378  CL109 HEK-293T C42(1.00)  LDD1582  [9]
 LDCM0379  CL11 HEK-293T C42(1.15)  LDD1583  [9]
 LDCM0381  CL111 HEK-293T C42(1.10)  LDD1585  [9]
 LDCM0383  CL113 HEK-293T C42(1.02)  LDD1587  [9]
 LDCM0385  CL115 HEK-293T C42(1.14)  LDD1589  [9]
 LDCM0387  CL117 HEK-293T C42(1.10)  LDD1591  [9]
 LDCM0389  CL119 HEK-293T C42(1.02)  LDD1593  [9]
 LDCM0390  CL12 HEK-293T C42(1.25)  LDD1594  [9]
 LDCM0392  CL121 HEK-293T C42(1.09)  LDD1596  [9]
 LDCM0394  CL123 HEK-293T C42(1.45)  LDD1598  [9]
 LDCM0396  CL125 HEK-293T C42(1.02)  LDD1600  [9]
 LDCM0398  CL127 HEK-293T C42(1.25)  LDD1602  [9]
 LDCM0400  CL13 HEK-293T C42(1.16)  LDD1604  [9]
 LDCM0402  CL15 HEK-293T C42(1.37)  LDD1606  [9]
 LDCM0404  CL17 HEK-293T C42(1.37)  LDD1608  [9]
 LDCM0406  CL19 HEK-293T C42(0.92)  LDD1610  [9]
 LDCM0411  CL23 HEK-293T C42(1.03)  LDD1615  [9]
 LDCM0412  CL24 HEK-293T C42(1.08)  LDD1616  [9]
 LDCM0413  CL25 HEK-293T C42(0.95)  LDD1617  [9]
 LDCM0415  CL27 HEK-293T C42(1.18)  LDD1619  [9]
 LDCM0417  CL29 HEK-293T C42(1.06)  LDD1621  [9]
 LDCM0418  CL3 HEK-293T C42(1.03)  LDD1622  [9]
 LDCM0420  CL31 HEK-293T C42(1.04)  LDD1624  [9]
 LDCM0424  CL35 HEK-293T C42(1.02)  LDD1628  [9]
 LDCM0425  CL36 HEK-293T C42(1.00)  LDD1629  [9]
 LDCM0426  CL37 HEK-293T C42(1.00)  LDD1630  [9]
 LDCM0428  CL39 HEK-293T C42(1.16)  LDD1632  [9]
 LDCM0431  CL41 HEK-293T C42(1.17)  LDD1635  [9]
 LDCM0433  CL43 HEK-293T C42(1.09)  LDD1637  [9]
 LDCM0437  CL47 HEK-293T C42(1.09)  LDD1641  [9]
 LDCM0438  CL48 HEK-293T C42(1.01)  LDD1642  [9]
 LDCM0439  CL49 HEK-293T C42(1.04)  LDD1643  [9]
 LDCM0440  CL5 HEK-293T C42(1.22)  LDD1644  [9]
 LDCM0444  CL53 HEK-293T C42(1.24)  LDD1647  [9]
 LDCM0446  CL55 HEK-293T C42(1.07)  LDD1649  [9]
 LDCM0450  CL59 HEK-293T C42(1.12)  LDD1653  [9]
 LDCM0452  CL60 HEK-293T C42(1.19)  LDD1655  [9]
 LDCM0453  CL61 HEK-293T C42(1.06)  LDD1656  [9]
 LDCM0455  CL63 HEK-293T C42(0.92)  LDD1658  [9]
 LDCM0457  CL65 HEK-293T C42(1.52)  LDD1660  [9]
 LDCM0459  CL67 HEK-293T C42(0.95)  LDD1662  [9]
 LDCM0462  CL7 HEK-293T C42(1.08)  LDD1665  [9]
 LDCM0464  CL71 HEK-293T C42(1.03)  LDD1667  [9]
 LDCM0465  CL72 HEK-293T C42(1.15)  LDD1668  [9]
 LDCM0466  CL73 HEK-293T C42(1.00)  LDD1669  [9]
 LDCM0470  CL77 HEK-293T C42(1.04)  LDD1673  [9]
 LDCM0472  CL79 HEK-293T C42(1.04)  LDD1675  [9]
 LDCM0477  CL83 HEK-293T C42(1.05)  LDD1680  [9]
 LDCM0478  CL84 HEK-293T C42(1.00)  LDD1681  [9]
 LDCM0479  CL85 HEK-293T C42(1.09)  LDD1682  [9]
 LDCM0481  CL87 HEK-293T C42(1.12)  LDD1684  [9]
 LDCM0483  CL89 HEK-293T C42(1.21)  LDD1686  [9]
 LDCM0486  CL91 HEK-293T C42(1.04)  LDD1689  [9]
 LDCM0490  CL95 HEK-293T C42(1.01)  LDD1693  [9]
 LDCM0491  CL96 HEK-293T C42(1.05)  LDD1694  [9]
 LDCM0492  CL97 HEK-293T C42(1.01)  LDD1695  [9]
 LDCM0494  CL99 HEK-293T C42(1.07)  LDD1697  [9]
 LDCM0495  E2913 HEK-293T C42(1.12)  LDD1698  [9]
 LDCM0213  Electrophilic fragment 2 MDA-MB-231 C42(1.13)  LDD1702  [3]
 LDCM0468  Fragment33 HEK-293T C42(1.03)  LDD1671  [9]
 LDCM0022  KB02 HEK-293T C42(1.04)  LDD1492  [9]
 LDCM0023  KB03 HEK-293T C42(1.06)  LDD1497  [9]
 LDCM0024  KB05 MEL167 C1537(1.39); C1658(1.45)  LDD3316  [2]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C1665(1.42)  LDD2097  [3]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C1665(1.77)  LDD2099  [3]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C1537(1.22)  LDD2120  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C1665(1.04)  LDD2125  [3]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C1665(0.88)  LDD2127  [3]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C1537(0.95); C1658(0.68)  LDD2136  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C1537(0.87); C1658(0.63)  LDD2137  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C1537(1.15)  LDD2140  [3]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C1537(1.28)  LDD2144  [3]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Collagen alpha-1(IV) chain (COL4A1) Type IV collagen family P02462

References

1 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
7 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402