Details of the Target

General Information of Target

Target ID LDTP00543
Target Name 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2)
Gene Name AGPAT2
Gene ID 10555
Synonyms
1-acyl-sn-glycerol-3-phosphate acyltransferase beta; EC 2.3.1.51; 1-acylglycerol-3-phosphate O-acyltransferase 2; 1-AGP acyltransferase 2; 1-AGPAT 2; Lysophosphatidic acid acyltransferase beta; LPAAT-beta
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MELWPCLAAALLLLLLLVQLSRAAEFYAKVALYCALCFTVSAVASLVCLLRHGGRTVENM
SIIGWFVRSFKYFYGLRFEVRDPRRLQEARPCVIVSNHQSILDMMGLMEVLPERCVQIAK
RELLFLGPVGLIMYLGGVFFINRQRSSTAMTVMADLGERMVRENLKVWIYPEGTRNDNGD
LLPFKKGAFYLAVQAQVPIVPVVYSSFSSFYNTKKKFFTSGTVTVQVLEAIPTSGLTAAD
VPALVDTCHRAMRTTFLHISKTPQENGATAGSGVQPAQ
Target Type
Literature-reported
Target Bioclass
Enzyme
Family
1-acyl-sn-glycerol-3-phosphate acyltransferase family
Subcellular location
Endoplasmic reticulum membrane
Function
Converts 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
TTD ID
T32821
Uniprot ID
O15120
DrugMap ID
TT9AYVR
Ensemble ID
ENST00000371694.7
HGNC ID
HGNC:325
ChEMBL ID
CHEMBL4772

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CHL1 SNV: p.V274L .
OVCAR5 SNV: p.A42S .
RKO SNV: p.A89P .
RL952 SNV: p.P5S .
SKOV3 SNV: p.T39P .
SW403 SNV: p.A268T .
SW620 SNV: p.T254N .
TE10 SNV: p.V202L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 6 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K185(0.61); K261(8.72)  LDD0277  [1]
DBIA
 Probe Info  		C248(1.20)  LDD3367  [2]
Jackson_1
 Probe Info  		2.34  LDD0121  [3]
IA-alkyne
 Probe Info  		C92(0.73)  LDD2207  [4]
AOyne
 Probe Info  		14.90  LDD0443  [5]
NAIA_5
 Probe Info  		N.A.  LDD2223  [6]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C285
 Probe Info  		19.43  LDD1955  [7]
Alk-rapa
 Probe Info  		3.73  LDD0213  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C248(1.07)  LDD1508  [9]
 LDCM0226  AC11 HEK-293T C248(0.86)  LDD1509  [9]
 LDCM0237  AC12 HEK-293T C248(0.93)  LDD1510  [9]
 LDCM0277  AC18 HEK-293T C248(0.94)  LDD1516  [9]
 LDCM0278  AC19 HEK-293T C248(1.00)  LDD1517  [9]
 LDCM0279  AC2 HEK-293T C248(1.12)  LDD1518  [9]
 LDCM0280  AC20 HEK-293T C248(1.09)  LDD1519  [9]
 LDCM0284  AC24 HEK-293T C248(1.67)  LDD1523  [9]
 LDCM0286  AC26 HEK-293T C248(1.05)  LDD1525  [9]
 LDCM0287  AC27 HEK-293T C248(0.90)  LDD1526  [9]
 LDCM0288  AC28 HEK-293T C248(0.97)  LDD1527  [9]
 LDCM0290  AC3 HEK-293T C248(0.78)  LDD1529  [9]
 LDCM0293  AC32 HEK-293T C248(0.94)  LDD1532  [9]
 LDCM0295  AC34 HEK-293T C248(0.95)  LDD1534  [9]
 LDCM0296  AC35 HEK-293T C248(0.81)  LDD1535  [9]
 LDCM0297  AC36 HEK-293T C248(1.08)  LDD1536  [9]
 LDCM0301  AC4 HEK-293T C248(0.93)  LDD1540  [9]
 LDCM0302  AC40 HEK-293T C248(1.08)  LDD1541  [9]
 LDCM0304  AC42 HEK-293T C248(1.10)  LDD1543  [9]
 LDCM0305  AC43 HEK-293T C248(0.82)  LDD1544  [9]
 LDCM0306  AC44 HEK-293T C248(1.06)  LDD1545  [9]
 LDCM0310  AC48 HEK-293T C248(1.50)  LDD1549  [9]
 LDCM0313  AC50 HEK-293T C248(1.08)  LDD1552  [9]
 LDCM0314  AC51 HEK-293T C248(0.91)  LDD1553  [9]
 LDCM0315  AC52 HEK-293T C248(1.04)  LDD1554  [9]
 LDCM0319  AC56 HEK-293T C248(1.02)  LDD1558  [9]
 LDCM0321  AC58 HEK-293T C248(1.11)  LDD1560  [9]
 LDCM0322  AC59 HEK-293T C248(0.89)  LDD1561  [9]
 LDCM0324  AC60 HEK-293T C248(1.23)  LDD1563  [9]
 LDCM0328  AC64 HEK-293T C248(0.91)  LDD1567  [9]
 LDCM0345  AC8 HEK-293T C248(0.87)  LDD1569  [9]
 LDCM0275  AKOS034007705 HEK-293T C248(1.12)  LDD1514  [9]
 LDCM0071  Cisar_cp14 MDA-MB-231 2.34  LDD0121  [3]
 LDCM0367  CL1 HEK-293T C248(0.96)  LDD1571  [9]
 LDCM0369  CL100 HEK-293T C248(0.78)  LDD1573  [9]
 LDCM0370  CL101 HEK-293T C248(0.96)  LDD1574  [9]
 LDCM0371  CL102 HEK-293T C248(0.99)  LDD1575  [9]
 LDCM0373  CL104 HEK-293T C248(0.86)  LDD1577  [9]
 LDCM0374  CL105 HEK-293T C248(1.21)  LDD1578  [9]
 LDCM0375  CL106 HEK-293T C248(0.87)  LDD1579  [9]
 LDCM0377  CL108 HEK-293T C248(0.80)  LDD1581  [9]
 LDCM0378  CL109 HEK-293T C248(1.16)  LDD1582  [9]
 LDCM0380  CL110 HEK-293T C248(0.65)  LDD1584  [9]
 LDCM0382  CL112 HEK-293T C248(0.92)  LDD1586  [9]
 LDCM0383  CL113 HEK-293T C248(1.01)  LDD1587  [9]
 LDCM0384  CL114 HEK-293T C248(0.95)  LDD1588  [9]
 LDCM0386  CL116 HEK-293T C248(0.86)  LDD1590  [9]
 LDCM0387  CL117 HEK-293T C248(0.89)  LDD1591  [9]
 LDCM0388  CL118 HEK-293T C248(1.02)  LDD1592  [9]
 LDCM0390  CL12 HEK-293T C248(1.38)  LDD1594  [9]
 LDCM0391  CL120 HEK-293T C248(0.95)  LDD1595  [9]
 LDCM0392  CL121 HEK-293T C248(0.89)  LDD1596  [9]
 LDCM0393  CL122 HEK-293T C248(0.95)  LDD1597  [9]
 LDCM0395  CL124 HEK-293T C248(0.99)  LDD1599  [9]
 LDCM0396  CL125 HEK-293T C248(1.11)  LDD1600  [9]
 LDCM0397  CL126 HEK-293T C248(1.08)  LDD1601  [9]
 LDCM0399  CL128 HEK-293T C248(1.05)  LDD1603  [9]
 LDCM0400  CL13 HEK-293T C248(1.15)  LDD1604  [9]
 LDCM0401  CL14 HEK-293T C248(1.01)  LDD1605  [9]
 LDCM0403  CL16 HEK-293T C248(0.84)  LDD1607  [9]
 LDCM0405  CL18 HEK-293T C248(1.24)  LDD1609  [9]
 LDCM0406  CL19 HEK-293T C248(0.78)  LDD1610  [9]
 LDCM0407  CL2 HEK-293T C248(1.11)  LDD1611  [9]
 LDCM0408  CL20 HEK-293T C248(1.17)  LDD1612  [9]
 LDCM0412  CL24 HEK-293T C248(1.27)  LDD1616  [9]
 LDCM0413  CL25 HEK-293T C248(1.12)  LDD1617  [9]
 LDCM0414  CL26 HEK-293T C248(0.97)  LDD1618  [9]
 LDCM0416  CL28 HEK-293T C248(0.77)  LDD1620  [9]
 LDCM0419  CL30 HEK-293T C248(0.97)  LDD1623  [9]
 LDCM0420  CL31 HEK-293T C248(0.91)  LDD1624  [9]
 LDCM0421  CL32 HEK-293T C248(0.94)  LDD1625  [9]
 LDCM0425  CL36 HEK-293T C248(1.11)  LDD1629  [9]
 LDCM0426  CL37 HEK-293T C248(0.89)  LDD1630  [9]
 LDCM0429  CL4 HEK-293T C248(0.97)  LDD1633  [9]
 LDCM0430  CL40 HEK-293T C248(1.10)  LDD1634  [9]
 LDCM0432  CL42 HEK-293T C248(1.02)  LDD1636  [9]
 LDCM0433  CL43 HEK-293T C248(0.72)  LDD1637  [9]
 LDCM0434  CL44 HEK-293T C248(1.01)  LDD1638  [9]
 LDCM0438  CL48 HEK-293T C248(0.86)  LDD1642  [9]
 LDCM0439  CL49 HEK-293T C248(1.19)  LDD1643  [9]
 LDCM0441  CL50 HEK-293T C248(0.87)  LDD1645  [9]
 LDCM0443  CL52 HEK-293T C248(1.32)  LDD1646  [9]
 LDCM0445  CL54 HEK-293T C248(0.97)  LDD1648  [9]
 LDCM0446  CL55 HEK-293T C248(0.85)  LDD1649  [9]
 LDCM0447  CL56 HEK-293T C248(1.18)  LDD1650  [9]
 LDCM0451  CL6 HEK-293T C248(1.11)  LDD1654  [9]
 LDCM0452  CL60 HEK-293T C248(1.13)  LDD1655  [9]
 LDCM0453  CL61 HEK-293T C248(1.09)  LDD1656  [9]
 LDCM0454  CL62 HEK-293T C248(1.03)  LDD1657  [9]
 LDCM0456  CL64 HEK-293T C248(0.77)  LDD1659  [9]
 LDCM0458  CL66 HEK-293T C248(1.15)  LDD1661  [9]
 LDCM0459  CL67 HEK-293T C248(0.86)  LDD1662  [9]
 LDCM0460  CL68 HEK-293T C248(0.83)  LDD1663  [9]
 LDCM0462  CL7 HEK-293T C248(0.83)  LDD1665  [9]
 LDCM0465  CL72 HEK-293T C248(1.36)  LDD1668  [9]
 LDCM0466  CL73 HEK-293T C248(1.02)  LDD1669  [9]
 LDCM0467  CL74 HEK-293T C248(1.08)  LDD1670  [9]
 LDCM0469  CL76 HEK-293T C248(0.78)  LDD1672  [9]
 LDCM0471  CL78 HEK-293T C248(1.02)  LDD1674  [9]
 LDCM0472  CL79 HEK-293T C248(0.81)  LDD1675  [9]
 LDCM0473  CL8 HEK-293T C248(0.82)  LDD1676  [9]
 LDCM0474  CL80 HEK-293T C248(0.98)  LDD1677  [9]
 LDCM0478  CL84 HEK-293T C248(1.24)  LDD1681  [9]
 LDCM0479  CL85 HEK-293T C248(1.43)  LDD1682  [9]
 LDCM0480  CL86 HEK-293T C248(0.90)  LDD1683  [9]
 LDCM0482  CL88 HEK-293T C248(1.05)  LDD1685  [9]
 LDCM0485  CL90 HEK-293T C248(0.92)  LDD1688  [9]
 LDCM0486  CL91 HEK-293T C248(0.94)  LDD1689  [9]
 LDCM0487  CL92 HEK-293T C248(1.04)  LDD1690  [9]
 LDCM0491  CL96 HEK-293T C248(1.01)  LDD1694  [9]
 LDCM0492  CL97 HEK-293T C248(0.96)  LDD1695  [9]
 LDCM0493  CL98 HEK-293T C248(0.98)  LDD1696  [9]
 LDCM0427  Fragment51 HEK-293T C248(0.93)  LDD1631  [9]
 LDCM0022  KB02 SNU-5 C248(1.44)  LDD2611  [2]
 LDCM0023  KB03 NUGC-3 C248(1.16)  LDD2950  [2]
 LDCM0024  KB05 NUGC-3 C248(1.20)  LDD3367  [2]
 LDCM0628  OTUB2-COV-1 HEK-293T C92(0.73)  LDD2207  [4]
 LDCM0090  Rapamycin JHH-7 3.73  LDD0213  [8]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Appendage and Scaffold Diverse Fully Functionalized Small-Molecule Probes via a Minimalist Terminal Alkyne-Aliphatic Diazirine Isocyanide. J Org Chem. 2018 Sep 21;83(18):11245-11253. doi: 10.1021/acs.joc.8b01831. Epub 2018 Aug 31.
4 Rapid Covalent-Probe Discovery by Electrophile-Fragment Screening. J Am Chem Soc. 2019 Jun 5;141(22):8951-8968. doi: 10.1021/jacs.9b02822. Epub 2019 May 22.
5 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
6 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Rapamycin targets STAT3 and impacts c-Myc to suppress tumor growth. Cell Chem Biol. 2022 Mar 17;29(3):373-385.e6. doi: 10.1016/j.chembiol.2021.10.006. Epub 2021 Oct 26.
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402