Details of the Target

General Information of Target

Target ID LDTP00268
Target Name Eukaryotic elongation factor 2 kinase (EEF2K)
Gene Name EEF2K
Gene ID 29904
Synonyms
Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; EC 2.7.11.20; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MADEDLIFRLEGVDGGQSPRAGHDGDSDGDSDDEEGYFICPITDDPSSNQNVNSKVNKYY
SNLTKSERYSSSGSPANSFHFKEAWKHAIQKAKHMPDPWAEFHLEDIATERATRHRYNAV
TGEWLDDEVLIKMASQPFGRGAMRECFRTKKLSNFLHAQQWKGASNYVAKRYIEPVDRDV
YFEDVRLQMEAKLWGEEYNRHKPPKQVDIMQMCIIELKDRPGKPLFHLEHYIEGKYIKYN
SNSGFVRDDNIRLTPQAFSHFTFERSGHQLIVVDIQGVGDLYTDPQIHTETGTDFGDGNL
GVRGMALFFYSHACNRICESMGLAPFDLSPRERDAVNQNTKLLQSAKTILRGTEEKCGSP
QVRTLSGSRPPLLRPLSENSGDENMSDVTFDSLPSSPSSATPHSQKLDHLHWPVFSDLDN
MASRDHDHLDNHRESENSGDSGYPSEKRGELDDPEPREHGHSYSNRKYESDEDSLGSSGR
VCVEKWNLLNSSRLHLPRASAVALEVQRLNALDLEKKIGKSILGKVHLAMVRYHEGGRFC
EKGEEWDQESAVFHLEHAANLGELEAIVGLGLMYSQLPHHILADVSLKETEENKTKGFDY
LLKAAEAGDRQSMILVARAFDSGQNLSPDRCQDWLEALHWYNTALEMTDCDEGGEYDGMQ
DEPRYMMLAREAEMLFTGGYGLEKDPQRSGDLYTQAAEAAMEAMKGRLANQYYQKAEEAW
AQMEE
Target Type
Successful
Target Bioclass
Enzyme
Family
Protein kinase superfamily, Alpha-type protein kinase family
Function
Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
TTD ID
T92494
Uniprot ID
O00418
DrugMap ID
TT1QFLA
Ensemble ID
ENST00000263026.10
HGNC ID
HGNC:24615
ChEMBL ID
CHEMBL5026

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0402  [1]
A-EBA
 Probe Info  		5.02  LDD0215  [2]
FBP2
 Probe Info  		4.17  LDD0323  [3]
Probe 1
 Probe Info  		Y59(20.69)  LDD3495  [4]
DBIA
 Probe Info  		C213(0.79)  LDD1507  [5]
ATP probe
 Probe Info  		K238(0.00); K93(0.00)  LDD0199  [6]
NAIA_4
 Probe Info  		N.A.  LDD2226  [7]
IPM
 Probe Info  		N.A.  LDD2156  [8]
AOyne
 Probe Info  		13.60  LDD0443  [9]
NAIA_5
 Probe Info  		N.A.  LDD2223  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C213(0.79)  LDD1507  [5]
 LDCM0215  AC10 HEK-293T C213(1.28)  LDD1508  [5]
 LDCM0226  AC11 HEK-293T C213(0.91); C318(1.00)  LDD1509  [5]
 LDCM0237  AC12 HEK-293T C213(0.98)  LDD1510  [5]
 LDCM0259  AC14 HEK-293T C213(1.00)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C213(0.98)  LDD1513  [5]
 LDCM0276  AC17 HEK-293T C213(1.07)  LDD1515  [5]
 LDCM0277  AC18 HEK-293T C213(0.92)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C213(0.94); C318(0.78)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C213(0.98)  LDD1518  [5]
 LDCM0280  AC20 HEK-293T C213(1.11)  LDD1519  [5]
 LDCM0282  AC22 HEK-293T C213(1.08)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C213(0.95)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C213(0.92)  LDD1523  [5]
 LDCM0285  AC25 HEK-293T C213(1.25)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C213(0.81)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C213(1.17); C318(0.80)  LDD1526  [5]
 LDCM0288  AC28 HEK-293T C213(1.06)  LDD1527  [5]
 LDCM0290  AC3 HEK-293T C213(0.78); C318(1.00)  LDD1529  [5]
 LDCM0291  AC30 HEK-293T C213(1.21)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C213(0.98)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C213(0.80)  LDD1532  [5]
 LDCM0294  AC33 HEK-293T C213(1.09)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C213(0.98)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C213(0.87); C318(1.14)  LDD1535  [5]
 LDCM0297  AC36 HEK-293T C213(0.86)  LDD1536  [5]
 LDCM0299  AC38 HEK-293T C213(0.98)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C213(1.15)  LDD1539  [5]
 LDCM0301  AC4 HEK-293T C213(1.07)  LDD1540  [5]
 LDCM0302  AC40 HEK-293T C213(0.81)  LDD1541  [5]
 LDCM0303  AC41 HEK-293T C213(1.10)  LDD1542  [5]
 LDCM0304  AC42 HEK-293T C213(1.17)  LDD1543  [5]
 LDCM0305  AC43 HEK-293T C213(0.97); C318(0.87)  LDD1544  [5]
 LDCM0306  AC44 HEK-293T C213(0.94)  LDD1545  [5]
 LDCM0308  AC46 HEK-293T C213(1.13)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C213(1.23)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C213(0.96)  LDD1549  [5]
 LDCM0311  AC49 HEK-293T C213(0.92)  LDD1550  [5]
 LDCM0313  AC50 HEK-293T C213(0.98)  LDD1552  [5]
 LDCM0314  AC51 HEK-293T C213(1.15); C318(1.09)  LDD1553  [5]
 LDCM0315  AC52 HEK-293T C213(1.08)  LDD1554  [5]
 LDCM0317  AC54 HEK-293T C213(0.95)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C213(1.23)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C213(0.88)  LDD1558  [5]
 LDCM0320  AC57 HEK-293T C213(1.06)  LDD1559  [5]
 LDCM0321  AC58 HEK-293T C213(0.78)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C213(1.15); C318(0.97)  LDD1561  [5]
 LDCM0323  AC6 HEK-293T C213(1.09)  LDD1562  [5]
 LDCM0324  AC60 HEK-293T C213(0.93)  LDD1563  [5]
 LDCM0326  AC62 HEK-293T C213(1.41)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C213(1.00)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C213(0.93)  LDD1567  [5]
 LDCM0334  AC7 HEK-293T C213(1.47)  LDD1568  [5]
 LDCM0345  AC8 HEK-293T C213(0.90)  LDD1569  [5]
 LDCM0356  AKOS034007680 HEK-293T C213(0.96)  LDD1570  [5]
 LDCM0275  AKOS034007705 HEK-293T C213(0.99)  LDD1514  [5]
 LDCM0156  Aniline NCI-H1299 13.68  LDD0403  [1]
 LDCM0367  CL1 HEK-293T C213(1.12)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C213(0.93)  LDD1572  [5]
 LDCM0370  CL101 HEK-293T C213(0.96)  LDD1574  [5]
 LDCM0372  CL103 HEK-293T C213(0.92)  LDD1576  [5]
 LDCM0374  CL105 HEK-293T C213(1.06)  LDD1578  [5]
 LDCM0376  CL107 HEK-293T C213(1.28)  LDD1580  [5]
 LDCM0378  CL109 HEK-293T C213(1.03)  LDD1582  [5]
 LDCM0379  CL11 HEK-293T C213(0.92)  LDD1583  [5]
 LDCM0381  CL111 HEK-293T C213(0.97)  LDD1585  [5]
 LDCM0383  CL113 HEK-293T C213(1.12)  LDD1587  [5]
 LDCM0385  CL115 HEK-293T C213(1.08)  LDD1589  [5]
 LDCM0387  CL117 HEK-293T C213(1.04)  LDD1591  [5]
 LDCM0389  CL119 HEK-293T C213(0.96)  LDD1593  [5]
 LDCM0390  CL12 HEK-293T C213(0.78)  LDD1594  [5]
 LDCM0392  CL121 HEK-293T C213(1.08)  LDD1596  [5]
 LDCM0394  CL123 HEK-293T C213(0.98)  LDD1598  [5]
 LDCM0396  CL125 HEK-293T C213(1.34)  LDD1600  [5]
 LDCM0398  CL127 HEK-293T C213(0.90)  LDD1602  [5]
 LDCM0400  CL13 HEK-293T C213(1.15)  LDD1604  [5]
 LDCM0402  CL15 HEK-293T C213(0.98)  LDD1606  [5]
 LDCM0404  CL17 HEK-293T C213(0.84)  LDD1608  [5]
 LDCM0405  CL18 HEK-293T C213(1.04)  LDD1609  [5]
 LDCM0406  CL19 HEK-293T C213(0.98); C318(0.84)  LDD1610  [5]
 LDCM0408  CL20 HEK-293T C213(0.82)  LDD1612  [5]
 LDCM0410  CL22 HEK-293T C213(0.88)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C213(0.96)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C213(0.75)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C213(1.09)  LDD1617  [5]
 LDCM0415  CL27 HEK-293T C213(0.91)  LDD1619  [5]
 LDCM0417  CL29 HEK-293T C213(0.98)  LDD1621  [5]
 LDCM0418  CL3 HEK-293T C213(1.25)  LDD1622  [5]
 LDCM0419  CL30 HEK-293T C213(0.91)  LDD1623  [5]
 LDCM0420  CL31 HEK-293T C213(0.81); C318(0.87)  LDD1624  [5]
 LDCM0421  CL32 HEK-293T C213(0.97)  LDD1625  [5]
 LDCM0423  CL34 HEK-293T C213(1.00)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C213(0.86)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C213(0.78)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C213(1.13)  LDD1630  [5]
 LDCM0428  CL39 HEK-293T C213(1.06)  LDD1632  [5]
 LDCM0431  CL41 HEK-293T C213(1.09)  LDD1635  [5]
 LDCM0432  CL42 HEK-293T C213(0.92)  LDD1636  [5]
 LDCM0433  CL43 HEK-293T C213(0.86); C318(0.93)  LDD1637  [5]
 LDCM0434  CL44 HEK-293T C213(0.83)  LDD1638  [5]
 LDCM0436  CL46 HEK-293T C213(0.80)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C213(1.04)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C213(0.83)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C213(1.30)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C213(1.00)  LDD1644  [5]
 LDCM0444  CL53 HEK-293T C213(0.92)  LDD1647  [5]
 LDCM0445  CL54 HEK-293T C213(0.86)  LDD1648  [5]
 LDCM0446  CL55 HEK-293T C213(0.87); C318(0.88)  LDD1649  [5]
 LDCM0447  CL56 HEK-293T C213(0.89)  LDD1650  [5]
 LDCM0449  CL58 HEK-293T C213(1.07)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C213(0.89)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C213(0.92)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C213(0.82)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C213(1.11)  LDD1656  [5]
 LDCM0455  CL63 HEK-293T C213(1.33)  LDD1658  [5]
 LDCM0457  CL65 HEK-293T C213(0.97)  LDD1660  [5]
 LDCM0458  CL66 HEK-293T C213(0.83)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C213(0.97); C318(0.88)  LDD1662  [5]
 LDCM0460  CL68 HEK-293T C213(0.85)  LDD1663  [5]
 LDCM0462  CL7 HEK-293T C213(0.94); C318(0.83)  LDD1665  [5]
 LDCM0463  CL70 HEK-293T C213(1.05)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C213(0.84)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C213(0.83)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C213(1.15)  LDD1669  [5]
 LDCM0470  CL77 HEK-293T C213(0.93)  LDD1673  [5]
 LDCM0471  CL78 HEK-293T C213(1.04)  LDD1674  [5]
 LDCM0472  CL79 HEK-293T C213(0.91); C318(0.97)  LDD1675  [5]
 LDCM0473  CL8 HEK-293T C213(0.69)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C213(0.81)  LDD1677  [5]
 LDCM0476  CL82 HEK-293T C213(0.94)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C213(0.81)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C213(0.81)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C213(1.56)  LDD1682  [5]
 LDCM0481  CL87 HEK-293T C213(1.28)  LDD1684  [5]
 LDCM0483  CL89 HEK-293T C213(1.15)  LDD1686  [5]
 LDCM0485  CL90 HEK-293T C213(0.63)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C213(1.00); C318(0.86)  LDD1689  [5]
 LDCM0487  CL92 HEK-293T C213(0.88)  LDD1690  [5]
 LDCM0489  CL94 HEK-293T C213(1.08)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C213(0.83)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C213(0.86)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C213(0.98)  LDD1695  [5]
 LDCM0494  CL99 HEK-293T C213(1.06)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C213(1.12)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C213(0.92)  LDD1671  [5]
 LDCM0022  KB02 KMS11 C314(1.67)  LDD2422  [10]
 LDCM0023  KB03 KMS11 C314(2.05)  LDD2839  [10]
 LDCM0024  KB05 KMS11 C314(1.96)  LDD3256  [10]

The Interaction Atlas With This Target

The Drug(s) Related To This Target

Approved
Click To Hide/Show 1 Drug(s) Interacting with This Target
Drug Name Drug Type External ID
Lapatinib Small molecular drug D08CDI

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 2-Ethynylbenzaldehyde-Based, Lysine-Targeting Irreversible Covalent Inhibitors for Protein Kinases and Nonkinases. J Am Chem Soc. 2023 Feb 12. doi: 10.1021/jacs.2c11595. Online ahead of print.
Mass spectrometry data entry: PXD037665
3 Tranylcypromine specificity for monoamine oxidase is limited by promiscuous protein labelling and lysosomal trapping. RSC Chem Biol. 2020 Aug 12;1(4):209-213. doi: 10.1039/d0cb00048e. eCollection 2020 Oct 1.
Mass spectrometry data entry: PXD018580
4 An Azo Coupling-Based Chemoproteomic Approach to Systematically Profile the Tyrosine Reactivity in the Human Proteome. Anal Chem. 2021 Jul 27;93(29):10334-10342. doi: 10.1021/acs.analchem.1c01935. Epub 2021 Jul 12.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
7 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
8 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
9 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
10 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840