Details of the Target

General Information of Target

Target ID LDTP14013
Target Name RWD domain-containing protein 3 (RWDD3)
Gene Name RWDD3
Gene ID 25950
Synonyms
RSUME; RWD domain-containing protein 3; RWD domain-containing sumoylation enhancer; RSUME
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MALRYPMAVGLNKGHKVTKNVSKPRHSRRRGRLTKHTKFVRDMIREVCGFAPYERRAMEL
LKVSKDKRALKFIKKRVGTHIRAKRKREELSNVLAAMRKAAAKKD
Target Bioclass
Enzyme
Subcellular location
Nucleus
Function
Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha (NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha (HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination.
Uniprot ID
Q9Y3V2
Ensemble ID
ENST00000263893.10
HGNC ID
HGNC:21393

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
NCIH1048 SNV: p.S28T .
SISO SNV: p.R171S .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C80(1.83)  LDD3409  [1]
IA-alkyne
 Probe Info  		C80(1.02)  LDD2187  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]
AOyne
 Probe Info  		15.00  LDD0443  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C80(0.97); C127(0.98)  LDD1507  [5]
 LDCM0215  AC10 HEK-293T C80(0.94); C127(1.32)  LDD1508  [5]
 LDCM0226  AC11 HEK-293T C80(0.96)  LDD1509  [5]
 LDCM0237  AC12 HEK-293T C80(1.00)  LDD1510  [5]
 LDCM0259  AC14 HEK-293T C80(1.02); C127(0.96)  LDD1512  [5]
 LDCM0270  AC15 HEK-293T C80(0.93); C127(0.97)  LDD1513  [5]
 LDCM0276  AC17 HEK-293T C80(1.02); C127(1.00)  LDD1515  [5]
 LDCM0277  AC18 HEK-293T C80(0.96); C127(1.29)  LDD1516  [5]
 LDCM0278  AC19 HEK-293T C80(1.03)  LDD1517  [5]
 LDCM0279  AC2 HEK-293T C80(0.94); C127(1.08)  LDD1518  [5]
 LDCM0280  AC20 HEK-293T C80(0.96)  LDD1519  [5]
 LDCM0281  AC21 HEK-293T C80(1.01); C127(0.93)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C80(1.00); C127(1.01)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C80(1.00); C127(1.43)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C80(0.98)  LDD1523  [5]
 LDCM0285  AC25 HEK-293T C80(1.01); C127(1.32)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C80(0.94); C127(0.82)  LDD1525  [5]
 LDCM0287  AC27 HEK-293T C80(0.90)  LDD1526  [5]
 LDCM0288  AC28 HEK-293T C80(1.00)  LDD1527  [5]
 LDCM0289  AC29 HEK-293T C80(1.10); C127(1.04)  LDD1528  [5]
 LDCM0290  AC3 HEK-293T C80(0.93)  LDD1529  [5]
 LDCM0291  AC30 HEK-293T C80(0.98); C127(0.94)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C80(0.92); C127(0.88)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C80(1.07)  LDD1532  [5]
 LDCM0294  AC33 HEK-293T C80(0.99); C127(1.32)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C80(0.95); C127(1.08)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C80(0.99)  LDD1535  [5]
 LDCM0297  AC36 HEK-293T C80(0.97)  LDD1536  [5]
 LDCM0298  AC37 HEK-293T C80(1.15); C127(1.00)  LDD1537  [5]
 LDCM0299  AC38 HEK-293T C80(0.99); C127(1.01)  LDD1538  [5]
 LDCM0300  AC39 HEK-293T C80(1.03); C127(0.97)  LDD1539  [5]
 LDCM0301  AC4 HEK-293T C80(0.97)  LDD1540  [5]
 LDCM0302  AC40 HEK-293T C80(1.01)  LDD1541  [5]
 LDCM0303  AC41 HEK-293T C80(1.04); C127(1.13)  LDD1542  [5]
 LDCM0304  AC42 HEK-293T C80(0.90); C127(1.13)  LDD1543  [5]
 LDCM0305  AC43 HEK-293T C80(1.02)  LDD1544  [5]
 LDCM0306  AC44 HEK-293T C80(1.03)  LDD1545  [5]
 LDCM0307  AC45 HEK-293T C80(1.04); C127(0.94)  LDD1546  [5]
 LDCM0308  AC46 HEK-293T C80(0.95); C127(1.51)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C80(1.07); C127(1.40)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C80(0.96)  LDD1549  [5]
 LDCM0311  AC49 HEK-293T C80(1.00); C127(0.93)  LDD1550  [5]
 LDCM0312  AC5 HEK-293T C80(1.04); C127(1.25)  LDD1551  [5]
 LDCM0313  AC50 HEK-293T C80(0.91); C127(1.06)  LDD1552  [5]
 LDCM0314  AC51 HEK-293T C80(0.97)  LDD1553  [5]
 LDCM0315  AC52 HEK-293T C80(0.94)  LDD1554  [5]
 LDCM0316  AC53 HEK-293T C80(1.18); C127(0.95)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C80(1.01); C127(0.96)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C80(0.99); C127(0.99)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C80(0.97)  LDD1558  [5]
 LDCM0320  AC57 HEK-293T C80(0.98); C127(1.01)  LDD1559  [5]
 LDCM0321  AC58 HEK-293T C80(1.00); C127(1.01)  LDD1560  [5]
 LDCM0322  AC59 HEK-293T C80(1.00)  LDD1561  [5]
 LDCM0323  AC6 HEK-293T C80(0.94); C127(1.13)  LDD1562  [5]
 LDCM0324  AC60 HEK-293T C80(0.99)  LDD1563  [5]
 LDCM0325  AC61 HEK-293T C80(1.05); C127(0.95)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C80(0.98); C127(0.84)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C80(1.00); C127(1.07)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C80(0.95)  LDD1567  [5]
 LDCM0334  AC7 HEK-293T C80(1.08); C127(0.95)  LDD1568  [5]
 LDCM0345  AC8 HEK-293T C80(1.04)  LDD1569  [5]
 LDCM0248  AKOS034007472 HEK-293T C80(1.07); C127(1.35)  LDD1511  [5]
 LDCM0356  AKOS034007680 HEK-293T C80(1.04); C127(1.00)  LDD1570  [5]
 LDCM0275  AKOS034007705 HEK-293T C80(1.04)  LDD1514  [5]
 LDCM0367  CL1 HEK-293T C80(1.02)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C80(0.97); C127(1.39)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C80(1.00); C127(0.86)  LDD1573  [5]
 LDCM0370  CL101 HEK-293T C80(1.11)  LDD1574  [5]
 LDCM0371  CL102 HEK-293T C80(1.00); C127(0.74)  LDD1575  [5]
 LDCM0372  CL103 HEK-293T C127(1.02)  LDD1576  [5]
 LDCM0373  CL104 HEK-293T C80(0.97); C127(0.96)  LDD1577  [5]
 LDCM0374  CL105 HEK-293T C80(1.00)  LDD1578  [5]
 LDCM0375  CL106 HEK-293T C80(1.06); C127(0.99)  LDD1579  [5]
 LDCM0376  CL107 HEK-293T C127(1.17)  LDD1580  [5]
 LDCM0377  CL108 HEK-293T C80(0.96); C127(0.93)  LDD1581  [5]
 LDCM0378  CL109 HEK-293T C80(1.02)  LDD1582  [5]
 LDCM0379  CL11 HEK-293T C80(0.91); C127(1.16)  LDD1583  [5]
 LDCM0380  CL110 HEK-293T C80(1.03); C127(0.98)  LDD1584  [5]
 LDCM0381  CL111 HEK-293T C127(1.14)  LDD1585  [5]
 LDCM0382  CL112 HEK-293T C80(0.94); C127(0.65)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C80(1.05)  LDD1587  [5]
 LDCM0384  CL114 HEK-293T C80(0.94); C127(0.98)  LDD1588  [5]
 LDCM0385  CL115 HEK-293T C127(0.84)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C80(0.95); C127(0.96)  LDD1590  [5]
 LDCM0387  CL117 HEK-293T C80(1.06)  LDD1591  [5]
 LDCM0388  CL118 HEK-293T C80(1.02); C127(0.75)  LDD1592  [5]
 LDCM0389  CL119 HEK-293T C127(1.14)  LDD1593  [5]
 LDCM0390  CL12 HEK-293T C80(1.02)  LDD1594  [5]
 LDCM0391  CL120 HEK-293T C80(0.96); C127(0.73)  LDD1595  [5]
 LDCM0392  CL121 HEK-293T C80(1.12)  LDD1596  [5]
 LDCM0393  CL122 HEK-293T C80(1.03); C127(1.04)  LDD1597  [5]
 LDCM0394  CL123 HEK-293T C127(1.08)  LDD1598  [5]
 LDCM0395  CL124 HEK-293T C80(0.99); C127(0.72)  LDD1599  [5]
 LDCM0396  CL125 HEK-293T C80(1.01)  LDD1600  [5]
 LDCM0397  CL126 HEK-293T C80(0.96); C127(0.92)  LDD1601  [5]
 LDCM0398  CL127 HEK-293T C127(1.48)  LDD1602  [5]
 LDCM0399  CL128 HEK-293T C80(1.00); C127(0.73)  LDD1603  [5]
 LDCM0400  CL13 HEK-293T C80(1.02)  LDD1604  [5]
 LDCM0401  CL14 HEK-293T C80(1.04); C127(0.94)  LDD1605  [5]
 LDCM0402  CL15 HEK-293T C127(0.65)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C80(0.97); C127(0.68)  LDD1607  [5]
 LDCM0404  CL17 HEK-293T C80(1.04); C127(1.04)  LDD1608  [5]
 LDCM0405  CL18 HEK-293T C80(0.91); C127(1.01)  LDD1609  [5]
 LDCM0406  CL19 HEK-293T C80(0.99)  LDD1610  [5]
 LDCM0407  CL2 HEK-293T C80(1.12); C127(1.08)  LDD1611  [5]
 LDCM0408  CL20 HEK-293T C80(0.96)  LDD1612  [5]
 LDCM0409  CL21 HEK-293T C80(1.07); C127(1.21)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C80(0.97); C127(1.16)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C80(1.03); C127(1.07)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C80(1.02)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C80(1.07)  LDD1617  [5]
 LDCM0414  CL26 HEK-293T C80(1.00); C127(0.84)  LDD1618  [5]
 LDCM0415  CL27 HEK-293T C127(1.08)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C80(0.97); C127(0.70)  LDD1620  [5]
 LDCM0417  CL29 HEK-293T C80(1.07); C127(0.98)  LDD1621  [5]
 LDCM0418  CL3 HEK-293T C127(0.89)  LDD1622  [5]
 LDCM0419  CL30 HEK-293T C80(1.05); C127(1.22)  LDD1623  [5]
 LDCM0420  CL31 HEK-293T C80(0.96)  LDD1624  [5]
 LDCM0421  CL32 HEK-293T C80(0.98)  LDD1625  [5]
 LDCM0422  CL33 HEK-293T C80(1.18); C127(1.03)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C80(0.98); C127(0.99)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C80(1.06); C127(1.16)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C80(1.06)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C80(1.05)  LDD1630  [5]
 LDCM0428  CL39 HEK-293T C127(1.04)  LDD1632  [5]
 LDCM0429  CL4 HEK-293T C80(0.93); C127(0.74)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C80(0.94); C127(0.88)  LDD1634  [5]
 LDCM0431  CL41 HEK-293T C80(0.98); C127(1.03)  LDD1635  [5]
 LDCM0432  CL42 HEK-293T C80(0.94); C127(0.93)  LDD1636  [5]
 LDCM0433  CL43 HEK-293T C80(1.06)  LDD1637  [5]
 LDCM0434  CL44 HEK-293T C80(1.04)  LDD1638  [5]
 LDCM0435  CL45 HEK-293T C80(1.05); C127(1.09)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C80(0.96); C127(0.90)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C80(1.12); C127(1.27)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C80(1.07)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C80(1.11)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C80(0.95); C127(0.97)  LDD1644  [5]
 LDCM0441  CL50 HEK-293T C80(1.07); C127(1.31)  LDD1645  [5]
 LDCM0443  CL52 HEK-293T C80(1.02); C127(0.90)  LDD1646  [5]
 LDCM0444  CL53 HEK-293T C80(1.03); C127(0.81)  LDD1647  [5]
 LDCM0445  CL54 HEK-293T C80(1.03); C127(1.05)  LDD1648  [5]
 LDCM0446  CL55 HEK-293T C80(1.04)  LDD1649  [5]
 LDCM0447  CL56 HEK-293T C80(0.95)  LDD1650  [5]
 LDCM0448  CL57 HEK-293T C80(1.10); C127(1.01)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C80(1.03); C127(1.12)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C80(1.10); C127(1.14)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C80(0.86); C127(1.19)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C80(1.04)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C80(1.03)  LDD1656  [5]
 LDCM0454  CL62 HEK-293T C80(1.18); C127(1.17)  LDD1657  [5]
 LDCM0455  CL63 HEK-293T C127(1.22)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C80(1.01); C127(0.59)  LDD1659  [5]
 LDCM0457  CL65 HEK-293T C80(1.00); C127(1.07)  LDD1660  [5]
 LDCM0458  CL66 HEK-293T C80(0.94); C127(1.25)  LDD1661  [5]
 LDCM0459  CL67 HEK-293T C80(1.04)  LDD1662  [5]
 LDCM0460  CL68 HEK-293T C80(1.03)  LDD1663  [5]
 LDCM0461  CL69 HEK-293T C80(1.14); C127(1.00)  LDD1664  [5]
 LDCM0462  CL7 HEK-293T C80(0.98)  LDD1665  [5]
 LDCM0463  CL70 HEK-293T C80(1.04); C127(1.04)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C80(1.02); C127(0.97)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C80(1.05)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C80(1.04)  LDD1669  [5]
 LDCM0467  CL74 HEK-293T C80(1.03); C127(1.08)  LDD1670  [5]
 LDCM0469  CL76 HEK-293T C80(0.95); C127(0.65)  LDD1672  [5]
 LDCM0470  CL77 HEK-293T C80(1.02); C127(0.95)  LDD1673  [5]
 LDCM0471  CL78 HEK-293T C80(0.93); C127(0.87)  LDD1674  [5]
 LDCM0472  CL79 HEK-293T C80(1.03)  LDD1675  [5]
 LDCM0473  CL8 HEK-293T C80(1.56)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C80(1.00)  LDD1677  [5]
 LDCM0475  CL81 HEK-293T C80(1.17); C127(1.34)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C80(1.02); C127(0.99)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C80(1.04); C127(1.11)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C80(1.02)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C80(1.05)  LDD1682  [5]
 LDCM0480  CL86 HEK-293T C80(0.96); C127(1.05)  LDD1683  [5]
 LDCM0481  CL87 HEK-293T C127(1.08)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C80(0.93); C127(0.80)  LDD1685  [5]
 LDCM0483  CL89 HEK-293T C80(1.00); C127(1.10)  LDD1686  [5]
 LDCM0484  CL9 HEK-293T C80(1.22); C127(1.02)  LDD1687  [5]
 LDCM0485  CL90 HEK-293T C80(0.93); C127(0.63)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C80(0.99)  LDD1689  [5]
 LDCM0487  CL92 HEK-293T C80(0.93)  LDD1690  [5]
 LDCM0488  CL93 HEK-293T C80(1.16); C127(1.03)  LDD1691  [5]
 LDCM0489  CL94 HEK-293T C80(0.96); C127(0.99)  LDD1692  [5]
 LDCM0490  CL95 HEK-293T C80(1.13); C127(0.37)  LDD1693  [5]
 LDCM0491  CL96 HEK-293T C80(1.02)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C80(1.08)  LDD1695  [5]
 LDCM0493  CL98 HEK-293T C80(1.03); C127(1.43)  LDD1696  [5]
 LDCM0494  CL99 HEK-293T C127(0.95)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C127(1.00)  LDD1698  [5]
 LDCM0625  F8 Ramos C80(1.02)  LDD2187  [2]
 LDCM0573  Fragment11 Ramos C80(3.46)  LDD2190  [2]
 LDCM0575  Fragment13 Ramos C80(1.12)  LDD2192  [2]
 LDCM0580  Fragment21 Ramos C80(1.18)  LDD2195  [2]
 LDCM0578  Fragment27 Ramos C80(1.10)  LDD2197  [2]
 LDCM0586  Fragment28 Ramos C80(0.75)  LDD2198  [2]
 LDCM0588  Fragment30 Ramos C80(1.33)  LDD2199  [2]
 LDCM0589  Fragment31 Ramos C80(1.78)  LDD2200  [2]
 LDCM0468  Fragment33 HEK-293T C127(1.11)  LDD1671  [5]
 LDCM0596  Fragment38 Ramos C80(1.23)  LDD2203  [2]
 LDCM0427  Fragment51 HEK-293T C80(1.08); C127(0.98)  LDD1631  [5]
 LDCM0610  Fragment52 Ramos C80(1.55)  LDD2204  [2]
 LDCM0614  Fragment56 Ramos C80(2.66)  LDD2205  [2]
 LDCM0022  KB02 HEK-293T C80(0.86)  LDD1492  [5]
 LDCM0023  KB03 HEK-293T C80(0.97)  LDD1497  [5]
 LDCM0024  KB05 RL95-2 C80(1.83)  LDD3409  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
SUMO-conjugating enzyme UBC9 (UBE2I) Ubiquitin-conjugating enzyme family P63279
Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Small ubiquitin-related modifier 1 (SUMO1) Ubiquitin family P63165

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402