Details of the Target

General Information of Target

Target ID LDTP13652
Target Name DNA polymerase iota (POLI)
Gene Name POLI
Gene ID 11201
Synonyms
RAD30B; DNA polymerase iota; EC 2.7.7.7; Eta2; RAD30 homolog B
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MRKELQLSLSVTLLLVCGFLYQFTLKSSCLFCLPSFKSHQGLEALLSHRRGIVFLETSER
MEPPHLVSCSVESAAKIYPEWPVVFFMKGLTDSTPMPSNSTYPAFSFLSAIDNVFLFPLD
MKRLLEDTPLFSWYNQINASAERNWLHISSDASRLAIIWKYGGIYMDTDVISIRPIPEEN
FLAAQASRYSSNGIFGFLPHHPFLWECMENFVEHYNSAIWGNQGPELMTRMLRVWCKLED
FQEVSDLRCLNISFLHPQRFYPISYREWRRYYEVWDTEPSFNVSYALHLWNHMNQEGRAV
IRGSNTLVENLYRKHCPRTYRDLIKGPEGSVTGELGPGNK
Target Bioclass
Enzyme
Family
DNA polymerase type-Y family
Subcellular location
Nucleus
Function
Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Uniprot ID
Q9UNA4
Ensemble ID
ENST00000579534.6
HGNC ID
HGNC:9182
ChEMBL ID
CHEMBL5391

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C336(0.99)  LDD0531  [1]
TFBX
 Probe Info  		N.A.  LDD0027  [2]
NAIA_5
 Probe Info  		N.A.  LDD2223  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C336(0.99)  LDD0531  [1]
 LDCM0237  AC12 HEK-293T C336(0.93)  LDD1510  [4]
 LDCM0259  AC14 HEK-293T C336(1.02)  LDD1512  [4]
 LDCM0270  AC15 HEK-293T C113(1.19)  LDD1513  [4]
 LDCM0276  AC17 HCT 116 C336(1.08)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C336(0.89)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C336(0.82)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C336(1.02)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C336(0.93)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C336(0.78)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C336(0.75)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C336(0.80)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C336(0.88)  LDD0601  [1]
 LDCM0285  AC25 HEK-293T C113(1.15)  LDD1524  [4]
 LDCM0288  AC28 HEK-293T C336(0.90)  LDD1527  [4]
 LDCM0289  AC29 HEK-293T C336(0.96)  LDD1528  [4]
 LDCM0290  AC3 HCT 116 C336(1.05)  LDD0607  [1]
 LDCM0291  AC30 HEK-293T C336(1.07)  LDD1530  [4]
 LDCM0292  AC31 HEK-293T C113(0.91)  LDD1531  [4]
 LDCM0294  AC33 HEK-293T C113(1.33)  LDD1533  [4]
 LDCM0297  AC36 HEK-293T C336(1.06)  LDD1536  [4]
 LDCM0298  AC37 HEK-293T C336(1.03)  LDD1537  [4]
 LDCM0299  AC38 HEK-293T C336(1.22)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C113(1.42)  LDD1539  [4]
 LDCM0301  AC4 HCT 116 C336(1.02)  LDD0618  [1]
 LDCM0303  AC41 HEK-293T C113(1.00)  LDD1542  [4]
 LDCM0306  AC44 HEK-293T C336(0.97)  LDD1545  [4]
 LDCM0307  AC45 HEK-293T C336(1.25)  LDD1546  [4]
 LDCM0308  AC46 HEK-293T C336(1.17)  LDD1547  [4]
 LDCM0309  AC47 HEK-293T C113(1.18)  LDD1548  [4]
 LDCM0311  AC49 HEK-293T C113(1.27)  LDD1550  [4]
 LDCM0312  AC5 HCT 116 C336(0.87)  LDD0629  [1]
 LDCM0315  AC52 HEK-293T C336(1.27)  LDD1554  [4]
 LDCM0316  AC53 HEK-293T C336(1.21)  LDD1555  [4]
 LDCM0317  AC54 HEK-293T C336(1.24)  LDD1556  [4]
 LDCM0318  AC55 HEK-293T C113(0.93)  LDD1557  [4]
 LDCM0320  AC57 HEK-293T C113(0.83)  LDD1559  [4]
 LDCM0323  AC6 HEK-293T C336(0.90)  LDD1562  [4]
 LDCM0324  AC60 HEK-293T C336(1.02)  LDD1563  [4]
 LDCM0325  AC61 HEK-293T C336(0.98)  LDD1564  [4]
 LDCM0326  AC62 HEK-293T C336(1.23)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C113(0.88)  LDD1566  [4]
 LDCM0334  AC7 HEK-293T C113(1.07)  LDD1568  [4]
 LDCM0349  AC83 HCT 116 C336(1.06)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C336(0.82)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C336(0.62)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C336(0.90)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C336(1.35)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C336(0.75)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C336(0.70)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C336(1.28)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C336(1.24)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C336(0.65)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C336(0.70)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C336(0.87)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C336(0.81)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C336(1.03)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C336(0.99)  LDD0681  [1]
 LDCM0248  AKOS034007472 HEK-293T C336(1.08)  LDD1511  [4]
 LDCM0356  AKOS034007680 HEK-293T C113(1.32)  LDD1570  [4]
 LDCM0632  CL-Sc Hep-G2 C336(20.00)  LDD2227  [3]
 LDCM0367  CL1 HEK-293T C336(1.13)  LDD1571  [4]
 LDCM0368  CL10 HEK-293T C336(1.35)  LDD1572  [4]
 LDCM0369  CL100 HCT 116 C336(1.14)  LDD0686  [1]
 LDCM0370  CL101 HEK-293T C336(1.05)  LDD1574  [4]
 LDCM0371  CL102 HEK-293T C113(1.04)  LDD1575  [4]
 LDCM0372  CL103 HEK-293T C336(1.32)  LDD1576  [4]
 LDCM0373  CL104 HEK-293T C336(0.93); C113(0.91)  LDD1577  [4]
 LDCM0374  CL105 HCT 116 C336(1.23)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C336(1.28)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C336(1.14)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C336(1.06)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C336(0.84)  LDD0695  [1]
 LDCM0379  CL11 HEK-293T C113(1.57)  LDD1583  [4]
 LDCM0380  CL110 HCT 116 C336(1.27)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C336(1.39)  LDD0698  [1]
 LDCM0382  CL112 HEK-293T C336(0.80); C113(1.01)  LDD1586  [4]
 LDCM0383  CL113 HEK-293T C336(1.15)  LDD1587  [4]
 LDCM0384  CL114 HEK-293T C113(1.13)  LDD1588  [4]
 LDCM0385  CL115 HEK-293T C336(1.28)  LDD1589  [4]
 LDCM0386  CL116 HEK-293T C336(0.97); C113(1.30)  LDD1590  [4]
 LDCM0387  CL117 HEK-293T C336(1.00)  LDD1591  [4]
 LDCM0388  CL118 HEK-293T C113(1.26)  LDD1592  [4]
 LDCM0389  CL119 HEK-293T C336(0.97)  LDD1593  [4]
 LDCM0391  CL120 HEK-293T C336(0.93); C113(1.26)  LDD1595  [4]
 LDCM0392  CL121 HEK-293T C336(1.24)  LDD1596  [4]
 LDCM0393  CL122 HEK-293T C113(1.07)  LDD1597  [4]
 LDCM0394  CL123 HEK-293T C336(1.31)  LDD1598  [4]
 LDCM0395  CL124 HEK-293T C336(1.26); C113(1.24)  LDD1599  [4]
 LDCM0396  CL125 HEK-293T C336(1.02)  LDD1600  [4]
 LDCM0397  CL126 HEK-293T C113(0.89)  LDD1601  [4]
 LDCM0398  CL127 HEK-293T C336(0.83)  LDD1602  [4]
 LDCM0399  CL128 HEK-293T C336(0.96); C113(1.20)  LDD1603  [4]
 LDCM0400  CL13 HEK-293T C336(0.97)  LDD1604  [4]
 LDCM0401  CL14 HEK-293T C113(0.87)  LDD1605  [4]
 LDCM0402  CL15 HEK-293T C336(1.11)  LDD1606  [4]
 LDCM0403  CL16 HCT 116 C336(0.73)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C336(0.92)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C336(0.75)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C336(0.85)  LDD0723  [1]
 LDCM0407  CL2 HEK-293T C113(1.18)  LDD1611  [4]
 LDCM0408  CL20 HCT 116 C336(1.10)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C336(1.12)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C336(1.03)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C336(1.01)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C336(0.96)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C336(0.95)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C336(0.96)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C336(0.81)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C336(1.05)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C336(1.15)  LDD0734  [1]
 LDCM0418  CL3 HEK-293T C336(0.98)  LDD1622  [4]
 LDCM0419  CL30 HCT 116 C336(0.88)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C336(0.83)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C336(1.22)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C336(1.04)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C336(1.11)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C336(0.87)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C336(1.00)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C336(1.02)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C336(1.97)  LDD0745  [1]
 LDCM0429  CL4 HEK-293T C336(0.85); C113(1.37)  LDD1633  [4]
 LDCM0430  CL40 HCT 116 C336(1.01)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C336(0.71)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C336(0.90)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C336(1.51)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C336(1.28)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C336(1.02)  LDD0752  [1]
 LDCM0436  CL46 HEK-293T C336(1.10)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C113(1.02)  LDD1641  [4]
 LDCM0439  CL49 HEK-293T C336(1.10)  LDD1643  [4]
 LDCM0440  CL5 HEK-293T C113(1.03)  LDD1644  [4]
 LDCM0441  CL50 HEK-293T C113(1.10)  LDD1645  [4]
 LDCM0443  CL52 HEK-293T C336(1.19); C113(1.16)  LDD1646  [4]
 LDCM0444  CL53 HEK-293T C113(1.38)  LDD1647  [4]
 LDCM0447  CL56 HEK-293T C336(1.01)  LDD1650  [4]
 LDCM0448  CL57 HEK-293T C336(1.33)  LDD1651  [4]
 LDCM0449  CL58 HEK-293T C336(1.74)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C113(1.04)  LDD1653  [4]
 LDCM0453  CL61 HCT 116 C336(1.25)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C336(1.13)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C336(0.93)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C336(0.74)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C336(1.07)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C336(1.33)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C336(1.22)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C336(0.83)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C336(0.72)  LDD0778  [1]
 LDCM0463  CL70 HCT 116 C336(0.65)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C336(1.02)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C336(1.10)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C336(0.75)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C336(1.00)  LDD0784  [1]
 LDCM0469  CL76 HEK-293T C336(0.89); C113(1.06)  LDD1672  [4]
 LDCM0470  CL77 HEK-293T C113(0.98)  LDD1673  [4]
 LDCM0473  CL8 HEK-293T C336(0.85)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C336(1.07)  LDD1677  [4]
 LDCM0475  CL81 HEK-293T C336(1.34)  LDD1678  [4]
 LDCM0476  CL82 HEK-293T C336(1.16)  LDD1679  [4]
 LDCM0477  CL83 HEK-293T C113(1.16)  LDD1680  [4]
 LDCM0479  CL85 HEK-293T C336(1.01)  LDD1682  [4]
 LDCM0480  CL86 HEK-293T C113(0.79)  LDD1683  [4]
 LDCM0481  CL87 HEK-293T C336(1.09)  LDD1684  [4]
 LDCM0482  CL88 HEK-293T C336(1.08); C113(1.07)  LDD1685  [4]
 LDCM0483  CL89 HEK-293T C113(1.21)  LDD1686  [4]
 LDCM0484  CL9 HEK-293T C336(1.15)  LDD1687  [4]
 LDCM0486  CL91 HCT 116 C336(0.89)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C336(0.80)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C336(0.89)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C336(1.11)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C336(0.88)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C336(1.01)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C336(1.12)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C336(1.05)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C336(1.19)  LDD0811  [1]
 LDCM0495  E2913 HEK-293T C336(1.34)  LDD1698  [4]
 LDCM0468  Fragment33 HCT 116 C336(1.17)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C336(1.53)  LDD0744  [1]
 LDCM0022  KB02 A204 C274(1.12); C336(1.83)  LDD2252  [5]
 LDCM0023  KB03 A204 C274(1.68); C336(2.93)  LDD2669  [5]
 LDCM0024  KB05 MKN45 C336(3.05)  LDD3332  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Histone acetyltransferase KAT5 (KAT5) MYST (SAS/MOZ) family Q92993
Protein kinase C alpha type (PRKCA) AGC Ser/Thr protein kinase family P17252
TNF receptor-associated factor 6 (TRAF6) TNF receptor-associated factor family Q9Y4K3
E3 ubiquitin-protein ligase TRIM39 (TRIM39) TRIM/RBCC family Q9HCM9
Transporter and channel
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein gamma (YWHAG) 14-3-3 family P61981
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger and BTB domain-containing protein 44 (ZBTB44) . Q8NCP5
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Homer protein homolog 3 (HOMER3) Homer family Q9NSC5
NEDD8 (NEDD8) Ubiquitin family Q15843
Polyubiquitin-C (UBC) Ubiquitin family P0CG48
DAZ-associated protein 2 (DAZAP2) . Q15038
DNA repair protein XRCC1 (XRCC1) . P18887
Transmembrane protein 239 (TMEM239) . Q8WW34

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
3 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840