Details of the Target

General Information of Target

Target ID LDTP13482
Target Name F-box only protein 4 (FBXO4)
Gene Name FBXO4
Gene ID 26272
Synonyms
FBX4; F-box only protein 4
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSRRPCSCALRPPRCSCSASPSAVTAAGRPRPSDSCKEESSTLSVKMKCDFNCNHVHSGL
KLVKPDDIGRLVSYTPAYLEGSCKDCIKDYERLSCIGSPIVSPRIVQLETESKRLHNKEN
QHVQQTLNSTNEIEALETSRLYEDSGYSSFSLQSGLSEHEEGSLLEENFGDSLQSCLLQI
QSPDQYPNKNLLPVLHFEKVVCSTLKKNAKRNPKVDREMLKEIIARGNFRLQNIIGRKMG
LECVDILSELFRRGLRHVLATILAQLSDMDLINVSKVSTTWKKILEDDKGAFQLYSKAIQ
RVTENNNKFSPHASTREYVMFRTPLASVQKSAAQTSLKKDAQTKLSNQGDQKGSTYSRHN
EFSEVAKTLKKNESLKACIRCNSPAKYDCYLQRATCKREGCGFDYCTKCLCNYHTTKDCS
DGKLLKASCKIGPLPGTKKSKKNLRRL
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. Promotes ubiquitination of FXR1 following phosphorylation of FXR1 by GSK3B, leading to FXR1 degradation by the proteasome.
Uniprot ID
Q9UKT5
Ensemble ID
ENST00000281623.8
HGNC ID
HGNC:13583

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 SNV: p.A56T .
DU145 SNV: p.A312V .
HCC15 SNV: p.K281E .
HT115 SNV: p.K126R .
MFE319 Insertion: p.L371FfsTer5 .
MOLM13 SNV: p.W99Ter .
RKO SNV: p.L328S .
SG231 SNV: p.L195V .
SUIT2 SNV: p.Y343H .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C147(391.15)  LDD0209  [1]
5E-2FA
 Probe Info  		N.A.  LDD2235  [2]
m-APA
 Probe Info  		N.A.  LDD2231  [2]
IA-alkyne
 Probe Info  		N.A.  LDD2241  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C146(1.05); C147(1.05)  LDD0812  [4]
 LDCM0215  AC10 HEK-293T C147(1.03)  LDD1508  [5]
 LDCM0237  AC12 HEK-293T C147(0.98)  LDD1510  [5]
 LDCM0246  AC128 HEK-293T C146(1.25); C147(1.25)  LDD0844  [4]
 LDCM0247  AC129 HEK-293T C146(1.12); C147(1.12)  LDD0845  [4]
 LDCM0249  AC130 HEK-293T C146(0.99); C147(0.99)  LDD0847  [4]
 LDCM0250  AC131 HEK-293T C146(1.19); C147(1.19)  LDD0848  [4]
 LDCM0251  AC132 HEK-293T C146(1.28); C147(1.28)  LDD0849  [4]
 LDCM0252  AC133 HEK-293T C146(1.22); C147(1.22)  LDD0850  [4]
 LDCM0253  AC134 HEK-293T C146(1.20); C147(1.20)  LDD0851  [4]
 LDCM0254  AC135 HEK-293T C146(1.46); C147(1.46)  LDD0852  [4]
 LDCM0255  AC136 HEK-293T C146(1.29); C147(1.29)  LDD0853  [4]
 LDCM0256  AC137 HEK-293T C146(1.32); C147(1.32)  LDD0854  [4]
 LDCM0257  AC138 HEK-293T C146(1.20); C147(1.20)  LDD0855  [4]
 LDCM0258  AC139 HEK-293T C146(1.41); C147(1.41)  LDD0856  [4]
 LDCM0259  AC14 HEK-293T C147(0.92)  LDD1512  [5]
 LDCM0260  AC140 HEK-293T C146(1.41); C147(1.41)  LDD0858  [4]
 LDCM0261  AC141 HEK-293T C146(1.34); C147(1.34)  LDD0859  [4]
 LDCM0262  AC142 HEK-293T C146(1.33); C147(1.33)  LDD0860  [4]
 LDCM0270  AC15 HEK-293T C147(0.94)  LDD1513  [5]
 LDCM0276  AC17 HEK-293T C147(1.06)  LDD1515  [5]
 LDCM0277  AC18 HEK-293T C147(0.97)  LDD1516  [5]
 LDCM0279  AC2 HEK-293T C146(1.08); C147(1.08)  LDD0877  [4]
 LDCM0280  AC20 HEK-293T C147(0.95)  LDD1519  [5]
 LDCM0281  AC21 HEK-293T C147(1.06)  LDD1520  [5]
 LDCM0282  AC22 HEK-293T C147(0.95)  LDD1521  [5]
 LDCM0283  AC23 HEK-293T C147(0.91)  LDD1522  [5]
 LDCM0284  AC24 HEK-293T C147(0.95)  LDD1523  [5]
 LDCM0285  AC25 HEK-293T C147(1.01)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C147(1.05)  LDD1525  [5]
 LDCM0288  AC28 HEK-293T C147(1.00)  LDD1527  [5]
 LDCM0289  AC29 HEK-293T C147(1.06)  LDD1528  [5]
 LDCM0290  AC3 HEK-293T C146(0.89); C147(0.89)  LDD0888  [4]
 LDCM0291  AC30 HEK-293T C147(0.96)  LDD1530  [5]
 LDCM0292  AC31 HEK-293T C147(0.96)  LDD1531  [5]
 LDCM0293  AC32 HEK-293T C147(0.96)  LDD1532  [5]
 LDCM0294  AC33 HEK-293T C147(0.97)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C147(0.99)  LDD1534  [5]
 LDCM0296  AC35 HEK-293T C146(0.83); C147(0.83)  LDD0894  [4]
 LDCM0297  AC36 HEK-293T C146(0.66); C147(0.66)  LDD0895  [4]
 LDCM0298  AC37 HEK-293T C146(0.84); C147(0.84)  LDD0896  [4]
 LDCM0299  AC38 HEK-293T C146(0.77); C147(0.77)  LDD0897  [4]
 LDCM0300  AC39 HEK-293T C146(0.74); C147(0.74)  LDD0898  [4]
 LDCM0301  AC4 HEK-293T C146(1.07); C147(1.07)  LDD0899  [4]
 LDCM0302  AC40 HEK-293T C146(0.83); C147(0.83)  LDD0900  [4]
 LDCM0303  AC41 HEK-293T C146(0.72); C147(0.72)  LDD0901  [4]
 LDCM0304  AC42 HEK-293T C146(0.78); C147(0.78)  LDD0902  [4]
 LDCM0305  AC43 HEK-293T C146(0.82); C147(0.82)  LDD0903  [4]
 LDCM0306  AC44 HEK-293T C146(0.93); C147(0.93)  LDD0904  [4]
 LDCM0307  AC45 HEK-293T C146(0.79); C147(0.79)  LDD0905  [4]
 LDCM0308  AC46 HEK-293T C147(1.00)  LDD1547  [5]
 LDCM0309  AC47 HEK-293T C147(1.04)  LDD1548  [5]
 LDCM0310  AC48 HEK-293T C147(0.97)  LDD1549  [5]
 LDCM0311  AC49 HEK-293T C147(1.01)  LDD1550  [5]
 LDCM0312  AC5 HEK-293T C146(1.06); C147(1.06)  LDD0910  [4]
 LDCM0313  AC50 HEK-293T C147(1.00)  LDD1552  [5]
 LDCM0315  AC52 HEK-293T C147(1.01)  LDD1554  [5]
 LDCM0316  AC53 HEK-293T C147(1.04)  LDD1555  [5]
 LDCM0317  AC54 HEK-293T C147(1.00)  LDD1556  [5]
 LDCM0318  AC55 HEK-293T C147(0.95)  LDD1557  [5]
 LDCM0319  AC56 HEK-293T C147(1.00)  LDD1558  [5]
 LDCM0320  AC57 HEK-293T C147(1.05)  LDD1559  [5]
 LDCM0321  AC58 HEK-293T C147(1.04)  LDD1560  [5]
 LDCM0323  AC6 HEK-293T C147(1.02)  LDD1562  [5]
 LDCM0324  AC60 HEK-293T C147(1.10)  LDD1563  [5]
 LDCM0325  AC61 HEK-293T C147(1.07)  LDD1564  [5]
 LDCM0326  AC62 HEK-293T C147(1.10)  LDD1565  [5]
 LDCM0327  AC63 HEK-293T C147(1.01)  LDD1566  [5]
 LDCM0328  AC64 HEK-293T C147(1.05)  LDD1567  [5]
 LDCM0332  AC68 HEK-293T C146(0.97); C147(0.97)  LDD0930  [4]
 LDCM0333  AC69 HEK-293T C146(0.95); C147(0.95)  LDD0931  [4]
 LDCM0334  AC7 HEK-293T C147(0.99)  LDD1568  [5]
 LDCM0335  AC70 HEK-293T C146(0.65); C147(0.65)  LDD0933  [4]
 LDCM0336  AC71 HEK-293T C146(0.80); C147(0.80)  LDD0934  [4]
 LDCM0337  AC72 HEK-293T C146(0.80); C147(0.80)  LDD0935  [4]
 LDCM0338  AC73 HEK-293T C146(0.81); C147(0.81)  LDD0936  [4]
 LDCM0339  AC74 HEK-293T C146(0.91); C147(0.91)  LDD0937  [4]
 LDCM0340  AC75 HEK-293T C146(0.82); C147(0.82)  LDD0938  [4]
 LDCM0341  AC76 HEK-293T C146(0.77); C147(0.77)  LDD0939  [4]
 LDCM0342  AC77 HEK-293T C146(0.84); C147(0.84)  LDD0940  [4]
 LDCM0343  AC78 HEK-293T C146(0.79); C147(0.79)  LDD0941  [4]
 LDCM0344  AC79 HEK-293T C146(1.17); C147(1.17)  LDD0942  [4]
 LDCM0345  AC8 HEK-293T C147(0.91)  LDD1569  [5]
 LDCM0346  AC80 HEK-293T C146(0.94); C147(0.94)  LDD0944  [4]
 LDCM0347  AC81 HEK-293T C146(1.20); C147(1.20)  LDD0945  [4]
 LDCM0348  AC82 HEK-293T C146(0.81); C147(0.81)  LDD0946  [4]
 LDCM0349  AC83 HEK-293T C146(1.16); C147(1.16)  LDD0947  [4]
 LDCM0350  AC84 HEK-293T C146(0.76); C147(0.76)  LDD0948  [4]
 LDCM0351  AC85 HEK-293T C146(0.89); C147(0.89)  LDD0949  [4]
 LDCM0352  AC86 HEK-293T C146(0.83); C147(0.83)  LDD0950  [4]
 LDCM0353  AC87 HEK-293T C146(0.83); C147(0.83)  LDD0951  [4]
 LDCM0354  AC88 HEK-293T C146(0.82); C147(0.82)  LDD0952  [4]
 LDCM0355  AC89 HEK-293T C146(1.44); C147(1.44)  LDD0953  [4]
 LDCM0357  AC90 HEK-293T C146(1.48); C147(1.48)  LDD0955  [4]
 LDCM0358  AC91 HEK-293T C146(0.82); C147(0.82)  LDD0956  [4]
 LDCM0359  AC92 HEK-293T C146(1.40); C147(1.40)  LDD0957  [4]
 LDCM0360  AC93 HEK-293T C146(0.91); C147(0.91)  LDD0958  [4]
 LDCM0361  AC94 HEK-293T C146(1.37); C147(1.37)  LDD0959  [4]
 LDCM0362  AC95 HEK-293T C146(1.00); C147(1.00)  LDD0960  [4]
 LDCM0363  AC96 HEK-293T C146(1.00); C147(1.00)  LDD0961  [4]
 LDCM0364  AC97 HEK-293T C146(1.60); C147(1.60)  LDD0962  [4]
 LDCM0248  AKOS034007472 HEK-293T C147(1.19)  LDD1511  [5]
 LDCM0356  AKOS034007680 HEK-293T C147(1.01)  LDD1570  [5]
 LDCM0275  AKOS034007705 HEK-293T C147(0.90)  LDD1514  [5]
 LDCM0367  CL1 HEK-293T C147(1.03)  LDD1571  [5]
 LDCM0368  CL10 HEK-293T C147(0.69)  LDD1572  [5]
 LDCM0369  CL100 HEK-293T C146(1.06); C147(1.06)  LDD0967  [4]
 LDCM0370  CL101 HEK-293T C147(1.15)  LDD1574  [5]
 LDCM0372  CL103 HEK-293T C147(0.98)  LDD1576  [5]
 LDCM0374  CL105 HEK-293T C147(1.04)  LDD1578  [5]
 LDCM0376  CL107 HEK-293T C147(0.90)  LDD1580  [5]
 LDCM0378  CL109 HEK-293T C147(1.07)  LDD1582  [5]
 LDCM0379  CL11 HEK-293T C147(0.71)  LDD1583  [5]
 LDCM0381  CL111 HEK-293T C147(0.94)  LDD1585  [5]
 LDCM0383  CL113 HEK-293T C147(1.03)  LDD1587  [5]
 LDCM0385  CL115 HEK-293T C147(1.01)  LDD1589  [5]
 LDCM0387  CL117 HEK-293T C146(0.88); C147(0.88)  LDD0985  [4]
 LDCM0388  CL118 HEK-293T C146(1.03); C147(1.03)  LDD0986  [4]
 LDCM0389  CL119 HEK-293T C146(0.90); C147(0.90)  LDD0987  [4]
 LDCM0390  CL12 HEK-293T C147(0.78)  LDD1594  [5]
 LDCM0391  CL120 HEK-293T C146(0.83); C147(0.83)  LDD0989  [4]
 LDCM0392  CL121 HEK-293T C147(1.06)  LDD1596  [5]
 LDCM0394  CL123 HEK-293T C147(1.10)  LDD1598  [5]
 LDCM0396  CL125 HEK-293T C147(1.09)  LDD1600  [5]
 LDCM0398  CL127 HEK-293T C147(1.07)  LDD1602  [5]
 LDCM0400  CL13 HEK-293T C147(1.10)  LDD1604  [5]
 LDCM0402  CL15 HEK-293T C147(1.04)  LDD1606  [5]
 LDCM0404  CL17 HEK-293T C147(0.88)  LDD1608  [5]
 LDCM0405  CL18 HEK-293T C147(0.91)  LDD1609  [5]
 LDCM0408  CL20 HEK-293T C147(0.75)  LDD1612  [5]
 LDCM0409  CL21 HEK-293T C147(0.66)  LDD1613  [5]
 LDCM0410  CL22 HEK-293T C147(0.65)  LDD1614  [5]
 LDCM0411  CL23 HEK-293T C147(0.71)  LDD1615  [5]
 LDCM0412  CL24 HEK-293T C147(0.76)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C147(1.06)  LDD1617  [5]
 LDCM0415  CL27 HEK-293T C147(0.90)  LDD1619  [5]
 LDCM0417  CL29 HEK-293T C147(0.86)  LDD1621  [5]
 LDCM0418  CL3 HEK-293T C147(0.99)  LDD1622  [5]
 LDCM0419  CL30 HEK-293T C147(0.88)  LDD1623  [5]
 LDCM0421  CL32 HEK-293T C147(0.78)  LDD1625  [5]
 LDCM0422  CL33 HEK-293T C147(0.68)  LDD1626  [5]
 LDCM0423  CL34 HEK-293T C147(0.66)  LDD1627  [5]
 LDCM0424  CL35 HEK-293T C147(0.70)  LDD1628  [5]
 LDCM0425  CL36 HEK-293T C147(0.76)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C147(0.97)  LDD1630  [5]
 LDCM0428  CL39 HEK-293T C147(1.00)  LDD1632  [5]
 LDCM0431  CL41 HEK-293T C147(0.90)  LDD1635  [5]
 LDCM0432  CL42 HEK-293T C147(0.98)  LDD1636  [5]
 LDCM0434  CL44 HEK-293T C147(0.80)  LDD1638  [5]
 LDCM0435  CL45 HEK-293T C147(0.79)  LDD1639  [5]
 LDCM0436  CL46 HEK-293T C147(0.70)  LDD1640  [5]
 LDCM0437  CL47 HEK-293T C147(0.74)  LDD1641  [5]
 LDCM0438  CL48 HEK-293T C147(0.69)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C147(1.09)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C147(0.88)  LDD1644  [5]
 LDCM0444  CL53 HEK-293T C147(0.96)  LDD1647  [5]
 LDCM0445  CL54 HEK-293T C147(1.08)  LDD1648  [5]
 LDCM0447  CL56 HEK-293T C147(0.80)  LDD1650  [5]
 LDCM0448  CL57 HEK-293T C147(0.80)  LDD1651  [5]
 LDCM0449  CL58 HEK-293T C147(0.68)  LDD1652  [5]
 LDCM0450  CL59 HEK-293T C147(0.71)  LDD1653  [5]
 LDCM0451  CL6 HEK-293T C147(1.09)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C147(0.75)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C147(1.08)  LDD1656  [5]
 LDCM0455  CL63 HEK-293T C147(0.87)  LDD1658  [5]
 LDCM0457  CL65 HEK-293T C147(0.94)  LDD1660  [5]
 LDCM0458  CL66 HEK-293T C147(1.02)  LDD1661  [5]
 LDCM0460  CL68 HEK-293T C147(0.77)  LDD1663  [5]
 LDCM0461  CL69 HEK-293T C147(0.72)  LDD1664  [5]
 LDCM0463  CL70 HEK-293T C147(0.71)  LDD1666  [5]
 LDCM0464  CL71 HEK-293T C147(0.74)  LDD1667  [5]
 LDCM0465  CL72 HEK-293T C147(0.72)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C147(1.01)  LDD1669  [5]
 LDCM0470  CL77 HEK-293T C147(1.00)  LDD1673  [5]
 LDCM0471  CL78 HEK-293T C147(0.94)  LDD1674  [5]
 LDCM0473  CL8 HEK-293T C147(0.80)  LDD1676  [5]
 LDCM0474  CL80 HEK-293T C147(0.79)  LDD1677  [5]
 LDCM0475  CL81 HEK-293T C147(0.74)  LDD1678  [5]
 LDCM0476  CL82 HEK-293T C147(0.73)  LDD1679  [5]
 LDCM0477  CL83 HEK-293T C147(0.72)  LDD1680  [5]
 LDCM0478  CL84 HEK-293T C147(0.74)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C147(1.06)  LDD1682  [5]
 LDCM0481  CL87 HEK-293T C147(1.00)  LDD1684  [5]
 LDCM0483  CL89 HEK-293T C147(1.01)  LDD1686  [5]
 LDCM0484  CL9 HEK-293T C147(0.76)  LDD1687  [5]
 LDCM0485  CL90 HEK-293T C147(1.35)  LDD1688  [5]
 LDCM0486  CL91 HEK-293T C146(0.98); C147(0.98)  LDD1084  [4]
 LDCM0487  CL92 HEK-293T C146(0.94); C147(0.94)  LDD1085  [4]
 LDCM0488  CL93 HEK-293T C146(1.15); C147(1.15)  LDD1086  [4]
 LDCM0489  CL94 HEK-293T C146(1.25); C147(1.25)  LDD1087  [4]
 LDCM0490  CL95 HEK-293T C146(1.11); C147(1.11)  LDD1088  [4]
 LDCM0491  CL96 HEK-293T C146(1.18); C147(1.18)  LDD1089  [4]
 LDCM0492  CL97 HEK-293T C146(1.19); C147(1.19)  LDD1090  [4]
 LDCM0493  CL98 HEK-293T C146(1.04); C147(1.04)  LDD1091  [4]
 LDCM0494  CL99 HEK-293T C146(0.95); C147(0.95)  LDD1092  [4]
 LDCM0495  E2913 HEK-293T C147(0.98)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C147(1.00)  LDD1671  [5]
 LDCM0022  KB02 HEK-293T C147(1.09)  LDD1492  [5]
 LDCM0023  KB03 Jurkat C147(391.15)  LDD0209  [1]
 LDCM0024  KB05 HEK-293T C147(1.04)  LDD1502  [5]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cullin-1 (CUL1) Cullin family Q13616
Serine/threonine-protein kinase TNNI3K (TNNI3K) TKL Ser/Thr protein kinase family Q59H18
S-phase kinase-associated protein 1 (SKP1) SKP1 family P63208
Transporter and channel
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
14-3-3 protein epsilon (YWHAE) 14-3-3 family P62258
Wolframin (WFS1) . O76024
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
TAR DNA-binding protein 43 (TARDBP) . Q13148
Telomeric repeat-binding factor 1 (TERF1) . P54274

References

1 Covalent Inhibition by a Natural Product-Inspired Latent Electrophile. J Am Chem Soc. 2023 May 24;145(20):11097-11109. doi: 10.1021/jacs.3c00598. Epub 2023 May 15.
2 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
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Mass spectrometry data entry: PXD044402