Details of the Target

General Information of Target

Target ID LDTP13415
Target Name ADP-ribosylation factor-binding protein GGA1 (GGA1)
Gene Name GGA1
Gene ID 26088
Synonyms
ADP-ribosylation factor-binding protein GGA1; Gamma-adaptin-related protein 1; Golgi-localized, gamma ear-containing, ARF-binding protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAATAVAAAVAGTESAQGPPGPAASLELWLNKATDPSMSEQDWSAIQNFCEQVNTDPNGP
THAPWLLAHKIQSPQEKEALYALTVLEMCMNHCGEKFHSEVAKFRFLNELIKVLSPKYLG
SWATGKVKGRVIEILFSWTVWFPEDIKIRDAYQMLKKQGIIKQDPKLPVDKILPPPSPWP
KSSIFDADEEKSKLLTRLLKSNHPEDLQAANRLIKNLVKEEQEKSEKVSKRVSAVEEVRS
HVKVLQEMLSMYRRPGQAPPDQEALQVVYERCEKLRPTLFRLASDTTDDDDALAEILQAN
DLLTQGVLLYKQVMEGRVTFGNRVTSSLGDIPVSRVFQNPAGCMKTCPLIDLEVDNGPAQ
MGTVVPSLLHQDLAALGISDAPVTGMVSGQNCCEEKRNPSSSTLPGGGVQNPSADRNLLD
LLSAQPAPCPLNYVSQKSVPKEVPPGTKSSPGWSWEAGPLAPSPSSQNTPLAQVFVPLES
VKPSSLPPLIVYDRNGFRILLHFSQTGAPGHPEVQVLLLTMMSTAPQPVWDIMFQVAVPK
SMRVKLQPASSSKLPAFSPLMPPAVISQMLLLDNPHKEPIRLRYKLTFNQGGQPFSEVGE
VKDFPDLAVLGAA
Target Bioclass
Transporter and channel
Family
GGA protein family
Subcellular location
Golgi apparatus, trans-Golgi network membrane
Function
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (DXXLL) motif. Mediates export of the GPCR receptor ADRA2B to the cell surface. Required for targeting PKD1:PKD2 complex from the trans-Golgi network to the cilium membrane. Regulates retrograde transport of proteins such as phosphorylated form of BACE1 from endosomes to the trans-Golgi network.
Uniprot ID
Q9UJY5
Ensemble ID
ENST00000325180.12
HGNC ID
HGNC:17842

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
CCK81 Insertion: p.R485QfsTer35 .
HCC1395 SNV: p.P484A .
HCT15 SNV: p.P581L .
IM95 SNV: p.R443W .
JURKAT SNV: p.R180H; p.S388L .
KMRC1 SNV: p.V564A .
MDST8 SNV: p.A276V .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 10 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K183(9.18)  LDD0277  [1]
m-APA
 Probe Info  		13.40  LDD0403  [2]
BTD
 Probe Info  		C256(0.46)  LDD2091  [3]
DA-P3
 Probe Info  		8.05  LDD0182  [4]
DBIA
 Probe Info  		C465(1.10)  LDD1507  [5]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [6]
IA-alkyne
 Probe Info  		N.A.  LDD0032  [7]
Lodoacetamide azide
 Probe Info  		N.A.  LDD0037  [6]
IPM
 Probe Info  		N.A.  LDD2156  [8]
NAIA_5
 Probe Info  		C34(0.00); C73(0.00)  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C256(1.09)  LDD2117  [3]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C256(0.98)  LDD2152  [3]
 LDCM0214  AC1 HEK-293T C465(1.10)  LDD1507  [5]
 LDCM0215  AC10 HEK-293T C465(1.04)  LDD1508  [5]
 LDCM0276  AC17 HEK-293T C465(0.99)  LDD1515  [5]
 LDCM0277  AC18 HEK-293T C465(0.77)  LDD1516  [5]
 LDCM0279  AC2 HEK-293T C465(0.80)  LDD1518  [5]
 LDCM0284  AC24 HEK-293T C465(1.04)  LDD1523  [5]
 LDCM0285  AC25 HEK-293T C465(0.93)  LDD1524  [5]
 LDCM0286  AC26 HEK-293T C465(0.76)  LDD1525  [5]
 LDCM0293  AC32 HEK-293T C465(0.89)  LDD1532  [5]
 LDCM0294  AC33 HEK-293T C465(0.83)  LDD1533  [5]
 LDCM0295  AC34 HEK-293T C465(0.76)  LDD1534  [5]
 LDCM0302  AC40 HEK-293T C465(1.05)  LDD1541  [5]
 LDCM0303  AC41 HEK-293T C465(0.72)  LDD1542  [5]
 LDCM0304  AC42 HEK-293T C465(1.04)  LDD1543  [5]
 LDCM0310  AC48 HEK-293T C465(0.97)  LDD1549  [5]
 LDCM0311  AC49 HEK-293T C465(0.91)  LDD1550  [5]
 LDCM0313  AC50 HEK-293T C465(0.79)  LDD1552  [5]
 LDCM0319  AC56 HEK-293T C465(0.76)  LDD1558  [5]
 LDCM0320  AC57 HEK-293T C465(1.26)  LDD1559  [5]
 LDCM0321  AC58 HEK-293T C465(0.81)  LDD1560  [5]
 LDCM0328  AC64 HEK-293T C465(0.72)  LDD1567  [5]
 LDCM0345  AC8 HEK-293T C465(1.37)  LDD1569  [5]
 LDCM0356  AKOS034007680 HEK-293T C465(0.90)  LDD1570  [5]
 LDCM0275  AKOS034007705 HEK-293T C465(0.84)  LDD1514  [5]
 LDCM0156  Aniline NCI-H1299 13.40  LDD0403  [2]
 LDCM0498  BS-3668 MDA-MB-231 C256(0.46)  LDD2091  [3]
 LDCM0367  CL1 HEK-293T C465(1.13)  LDD1571  [5]
 LDCM0369  CL100 HEK-293T C465(1.01)  LDD1573  [5]
 LDCM0370  CL101 HEK-293T C465(1.11)  LDD1574  [5]
 LDCM0372  CL103 HEK-293T C465(0.89)  LDD1576  [5]
 LDCM0373  CL104 HEK-293T C465(0.78)  LDD1577  [5]
 LDCM0374  CL105 HEK-293T C465(1.12)  LDD1578  [5]
 LDCM0376  CL107 HEK-293T C465(1.00)  LDD1580  [5]
 LDCM0377  CL108 HEK-293T C465(0.97)  LDD1581  [5]
 LDCM0378  CL109 HEK-293T C465(0.72)  LDD1582  [5]
 LDCM0381  CL111 HEK-293T C465(1.06)  LDD1585  [5]
 LDCM0382  CL112 HEK-293T C465(0.91)  LDD1586  [5]
 LDCM0383  CL113 HEK-293T C465(1.15)  LDD1587  [5]
 LDCM0385  CL115 HEK-293T C465(1.02)  LDD1589  [5]
 LDCM0386  CL116 HEK-293T C465(1.01)  LDD1590  [5]
 LDCM0387  CL117 HEK-293T C465(0.93)  LDD1591  [5]
 LDCM0389  CL119 HEK-293T C465(0.92)  LDD1593  [5]
 LDCM0390  CL12 HEK-293T C465(1.61)  LDD1594  [5]
 LDCM0391  CL120 HEK-293T C465(0.86)  LDD1595  [5]
 LDCM0392  CL121 HEK-293T C465(0.85)  LDD1596  [5]
 LDCM0394  CL123 HEK-293T C465(0.88)  LDD1598  [5]
 LDCM0395  CL124 HEK-293T C465(0.76)  LDD1599  [5]
 LDCM0396  CL125 HEK-293T C465(0.85)  LDD1600  [5]
 LDCM0398  CL127 HEK-293T C465(0.88)  LDD1602  [5]
 LDCM0399  CL128 HEK-293T C465(0.63)  LDD1603  [5]
 LDCM0400  CL13 HEK-293T C465(1.03)  LDD1604  [5]
 LDCM0402  CL15 HEK-293T C465(0.89)  LDD1606  [5]
 LDCM0403  CL16 HEK-293T C465(1.08)  LDD1607  [5]
 LDCM0404  CL17 HEK-293T C465(0.94)  LDD1608  [5]
 LDCM0405  CL18 HEK-293T C465(0.86)  LDD1609  [5]
 LDCM0412  CL24 HEK-293T C465(1.57)  LDD1616  [5]
 LDCM0413  CL25 HEK-293T C465(0.82)  LDD1617  [5]
 LDCM0415  CL27 HEK-293T C465(0.98)  LDD1619  [5]
 LDCM0416  CL28 HEK-293T C465(1.07)  LDD1620  [5]
 LDCM0417  CL29 HEK-293T C465(1.15)  LDD1621  [5]
 LDCM0418  CL3 HEK-293T C465(1.10)  LDD1622  [5]
 LDCM0419  CL30 HEK-293T C465(1.16)  LDD1623  [5]
 LDCM0425  CL36 HEK-293T C465(1.64)  LDD1629  [5]
 LDCM0426  CL37 HEK-293T C465(1.56)  LDD1630  [5]
 LDCM0428  CL39 HEK-293T C465(0.94)  LDD1632  [5]
 LDCM0429  CL4 HEK-293T C465(0.98)  LDD1633  [5]
 LDCM0430  CL40 HEK-293T C465(1.29)  LDD1634  [5]
 LDCM0431  CL41 HEK-293T C465(0.75)  LDD1635  [5]
 LDCM0432  CL42 HEK-293T C465(1.21)  LDD1636  [5]
 LDCM0438  CL48 HEK-293T C465(1.51)  LDD1642  [5]
 LDCM0439  CL49 HEK-293T C465(0.91)  LDD1643  [5]
 LDCM0440  CL5 HEK-293T C465(1.07)  LDD1644  [5]
 LDCM0443  CL52 HEK-293T C465(1.21)  LDD1646  [5]
 LDCM0444  CL53 HEK-293T C465(0.78)  LDD1647  [5]
 LDCM0445  CL54 HEK-293T C465(0.74)  LDD1648  [5]
 LDCM0451  CL6 HEK-293T C465(1.03)  LDD1654  [5]
 LDCM0452  CL60 HEK-293T C465(1.57)  LDD1655  [5]
 LDCM0453  CL61 HEK-293T C465(0.81)  LDD1656  [5]
 LDCM0455  CL63 HEK-293T C465(1.03)  LDD1658  [5]
 LDCM0456  CL64 HEK-293T C465(0.82)  LDD1659  [5]
 LDCM0457  CL65 HEK-293T C465(1.30)  LDD1660  [5]
 LDCM0458  CL66 HEK-293T C465(0.87)  LDD1661  [5]
 LDCM0465  CL72 HEK-293T C465(1.41)  LDD1668  [5]
 LDCM0466  CL73 HEK-293T C465(0.91)  LDD1669  [5]
 LDCM0469  CL76 HEK-293T C465(1.10)  LDD1672  [5]
 LDCM0470  CL77 HEK-293T C465(0.89)  LDD1673  [5]
 LDCM0471  CL78 HEK-293T C465(1.13)  LDD1674  [5]
 LDCM0478  CL84 HEK-293T C465(1.56)  LDD1681  [5]
 LDCM0479  CL85 HEK-293T C465(0.89)  LDD1682  [5]
 LDCM0481  CL87 HEK-293T C465(0.85)  LDD1684  [5]
 LDCM0482  CL88 HEK-293T C465(0.77)  LDD1685  [5]
 LDCM0483  CL89 HEK-293T C465(0.86)  LDD1686  [5]
 LDCM0485  CL90 HEK-293T C465(0.80)  LDD1688  [5]
 LDCM0491  CL96 HEK-293T C465(0.92)  LDD1694  [5]
 LDCM0492  CL97 HEK-293T C465(0.83)  LDD1695  [5]
 LDCM0494  CL99 HEK-293T C465(0.87)  LDD1697  [5]
 LDCM0495  E2913 HEK-293T C465(1.06)  LDD1698  [5]
 LDCM0468  Fragment33 HEK-293T C465(0.89)  LDD1671  [5]
 LDCM0030  Luteolin HEK-293T 8.05  LDD0182  [4]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C256(1.02)  LDD2093  [3]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C256(0.76)  LDD2107  [3]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C256(1.02)  LDD2111  [3]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C256(2.53)  LDD2119  [3]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C256(0.86)  LDD2125  [3]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C256(0.88)  LDD2129  [3]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C256(0.56)  LDD2133  [3]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C256(0.95)  LDD2137  [3]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C256(1.19)  LDD2140  [3]
 LDCM0550  Nucleophilic fragment 5a MDA-MB-231 C256(2.95)  LDD2144  [3]
 LDCM0032  Oleacein HEK-293T 4.53  LDD0184  [4]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Dystrobrevin beta (DTNB) Dystrophin family O60941
Beta-secretase 1 (BACE1) Peptidase A1 family P56817
Transporter and channel
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Amyloid-beta precursor protein (APP) APP family P05067
ADP-ribosylation factor-binding protein GGA3 (GGA3) GGA protein family Q9NZ52
Junctophilin-3 (JPH3) Junctophilin family Q8WXH2
Cation-independent mannose-6-phosphate receptor (IGF2R) MRL1/IGF2R family P11717
Sortilin-related receptor (SORL1) VPS10-related sortilin family Q92673
Sortilin (SORT1) VPS10-related sortilin family Q99523
Other
Click To Hide/Show 8 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
ADP-ribosylation factor-binding protein GGA2 (GGA2) GGA protein family Q9UJY4
Protein DGCR6L (DGCR6L) Gonadal family Q9BY27
Inhibitor of growth protein 5 (ING5) ING family Q8WYH8
Protein MON2 homolog (MON2) MON2 family Q7Z3U7
Rab GTPase-binding effector protein 1 (RABEP1) Rabaptin family Q15276
Polyubiquitin-B (UBB) Ubiquitin family P0CG47
UPF0500 protein C1orf216 (C1orf216) UPF0500 family Q8TAB5
RING finger protein 11 (RNF11) . Q9Y3C5

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
3 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
4 A chemical probe unravels the reactive proteome of health-associated catechols. Chem Sci. 2023 Jul 22;14(32):8635-8643. doi: 10.1039/d3sc00888f. eCollection 2023 Aug 16.
Mass spectrometry data entry: PXD043348
5 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
6 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
7 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
8 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264