Details of the Target

General Information of Target

Target ID LDTP13001
Target Name DNA-directed RNA polymerase I subunit RPA12 (POLR1H)
Gene Name POLR1H
Gene ID 30834
Synonyms
RPA12; ZNRD1; DNA-directed RNA polymerase I subunit RPA12; DNA-directed RNA polymerase I subunit H; Zinc ribbon domain-containing protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSGAGEALAPGPVGPQRVAEAGGGQLGSTAQGKCDKDNTEKDITQATNSHFTHGEMQDQS
IWGNPSDGELIRTQPQRLPQLQTSAQVPSGEEIGKIKNGHTGLSNGNGIHHGAKHGSADN
RKLSAPVSQKMHRKIQSSLSVNSDISKKSKVNAVFSQKTGSSPEDCCVHCILACLFCEFL
TLCNIVLGQASCGICTSEACCCCCGDEMGDDCNCPCDMDCGIMDACCESSDCLEICMECC
GICFPS
Target Bioclass
Enzyme
Family
Archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family
Subcellular location
Nucleus, nucleolus
Function
Core component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates. Can mediate Pol I proofreading of the nascent RNA transcript. Anchors into the Pol I active site to monitor transcription fidelity and cleave mis-incorporated 5'-ribonucleotides.
Uniprot ID
Q9P1U0
Ensemble ID
ENST00000332435.10
HGNC ID
HGNC:13182

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 9 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
m-APA
 Probe Info  		15.00  LDD0403  [1]
DBIA
 Probe Info  		C41(1.03)  LDD3312  [2]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [3]
IA-alkyne
 Probe Info  		N.A.  LDD0162  [4]
NAIA_4
 Probe Info  		N.A.  LDD2226  [5]
NAIA_5
 Probe Info  		N.A.  LDD2224  [5]
IPM
 Probe Info  		N.A.  LDD2156  [6]
TFBX
 Probe Info  		C90(0.00); C41(0.00)  LDD0148  [7]
Phosphinate-6
 Probe Info  		N.A.  LDD0018  [8]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C78(1.01)  LDD1507  [9]
 LDCM0215  AC10 HEK-293T C78(1.12)  LDD1508  [9]
 LDCM0226  AC11 HEK-293T C78(1.08)  LDD1509  [9]
 LDCM0237  AC12 HEK-293T C78(1.10)  LDD1510  [9]
 LDCM0259  AC14 HEK-293T C78(1.15)  LDD1512  [9]
 LDCM0270  AC15 HEK-293T C78(1.33)  LDD1513  [9]
 LDCM0276  AC17 HEK-293T C78(1.29)  LDD1515  [9]
 LDCM0277  AC18 HEK-293T C78(1.08)  LDD1516  [9]
 LDCM0278  AC19 HEK-293T C78(1.07)  LDD1517  [9]
 LDCM0279  AC2 HEK-293T C78(1.03)  LDD1518  [9]
 LDCM0280  AC20 HEK-293T C78(1.05)  LDD1519  [9]
 LDCM0281  AC21 HEK-293T C78(0.90)  LDD1520  [9]
 LDCM0282  AC22 HEK-293T C78(1.21)  LDD1521  [9]
 LDCM0283  AC23 HEK-293T C78(1.33)  LDD1522  [9]
 LDCM0284  AC24 HEK-293T C78(1.13)  LDD1523  [9]
 LDCM0285  AC25 HEK-293T C78(1.01)  LDD1524  [9]
 LDCM0286  AC26 HEK-293T C78(1.09)  LDD1525  [9]
 LDCM0287  AC27 HEK-293T C78(1.12)  LDD1526  [9]
 LDCM0288  AC28 HEK-293T C78(1.07)  LDD1527  [9]
 LDCM0289  AC29 HEK-293T C78(0.88)  LDD1528  [9]
 LDCM0290  AC3 HEK-293T C78(0.88)  LDD1529  [9]
 LDCM0291  AC30 HEK-293T C78(0.97)  LDD1530  [9]
 LDCM0292  AC31 HEK-293T C78(1.24)  LDD1531  [9]
 LDCM0293  AC32 HEK-293T C78(1.09)  LDD1532  [9]
 LDCM0294  AC33 HEK-293T C78(1.22)  LDD1533  [9]
 LDCM0295  AC34 HEK-293T C78(0.95)  LDD1534  [9]
 LDCM0296  AC35 HEK-293T C78(1.08)  LDD1535  [9]
 LDCM0297  AC36 HEK-293T C78(1.04)  LDD1536  [9]
 LDCM0298  AC37 HEK-293T C78(0.89)  LDD1537  [9]
 LDCM0299  AC38 HEK-293T C78(1.04)  LDD1538  [9]
 LDCM0300  AC39 HEK-293T C78(1.27)  LDD1539  [9]
 LDCM0301  AC4 HEK-293T C78(0.94)  LDD1540  [9]
 LDCM0302  AC40 HEK-293T C78(1.10)  LDD1541  [9]
 LDCM0303  AC41 HEK-293T C78(1.21)  LDD1542  [9]
 LDCM0304  AC42 HEK-293T C78(1.34)  LDD1543  [9]
 LDCM0305  AC43 HEK-293T C78(1.21)  LDD1544  [9]
 LDCM0306  AC44 HEK-293T C78(1.06)  LDD1545  [9]
 LDCM0307  AC45 HEK-293T C78(1.09)  LDD1546  [9]
 LDCM0308  AC46 HEK-293T C78(1.18)  LDD1547  [9]
 LDCM0309  AC47 HEK-293T C78(1.25)  LDD1548  [9]
 LDCM0310  AC48 HEK-293T C78(1.06)  LDD1549  [9]
 LDCM0311  AC49 HEK-293T C78(1.04)  LDD1550  [9]
 LDCM0312  AC5 HEK-293T C78(0.90)  LDD1551  [9]
 LDCM0313  AC50 HEK-293T C78(1.02)  LDD1552  [9]
 LDCM0314  AC51 HEK-293T C78(1.14)  LDD1553  [9]
 LDCM0315  AC52 HEK-293T C78(1.02)  LDD1554  [9]
 LDCM0316  AC53 HEK-293T C78(0.99)  LDD1555  [9]
 LDCM0317  AC54 HEK-293T C78(1.07)  LDD1556  [9]
 LDCM0318  AC55 HEK-293T C78(1.37)  LDD1557  [9]
 LDCM0319  AC56 HEK-293T C78(1.01)  LDD1558  [9]
 LDCM0320  AC57 HEK-293T C78(1.18)  LDD1559  [9]
 LDCM0321  AC58 HEK-293T C78(1.18)  LDD1560  [9]
 LDCM0322  AC59 HEK-293T C78(1.03)  LDD1561  [9]
 LDCM0323  AC6 HEK-293T C78(0.91)  LDD1562  [9]
 LDCM0324  AC60 HEK-293T C78(1.08)  LDD1563  [9]
 LDCM0325  AC61 HEK-293T C78(0.95)  LDD1564  [9]
 LDCM0326  AC62 HEK-293T C78(1.07)  LDD1565  [9]
 LDCM0327  AC63 HEK-293T C78(1.29)  LDD1566  [9]
 LDCM0328  AC64 HEK-293T C78(1.04)  LDD1567  [9]
 LDCM0334  AC7 HEK-293T C78(1.04)  LDD1568  [9]
 LDCM0345  AC8 HEK-293T C78(0.89)  LDD1569  [9]
 LDCM0248  AKOS034007472 HEK-293T C78(0.96)  LDD1511  [9]
 LDCM0356  AKOS034007680 HEK-293T C78(1.24)  LDD1570  [9]
 LDCM0275  AKOS034007705 HEK-293T C78(1.02)  LDD1514  [9]
 LDCM0156  Aniline NCI-H1299 15.00  LDD0403  [1]
 LDCM0020  ARS-1620 HCC44 C41(1.25)  LDD2171  [10]
 LDCM0632  CL-Sc Hep-G2 C78(20.00)  LDD2227  [5]
 LDCM0368  CL10 HEK-293T C78(0.86)  LDD1572  [9]
 LDCM0369  CL100 HEK-293T C78(0.92)  LDD1573  [9]
 LDCM0371  CL102 HEK-293T C78(1.28)  LDD1575  [9]
 LDCM0372  CL103 HEK-293T C78(1.16)  LDD1576  [9]
 LDCM0373  CL104 HEK-293T C78(1.04)  LDD1577  [9]
 LDCM0375  CL106 HEK-293T C78(1.18)  LDD1579  [9]
 LDCM0376  CL107 HEK-293T C78(0.95)  LDD1580  [9]
 LDCM0377  CL108 HEK-293T C78(1.07)  LDD1581  [9]
 LDCM0379  CL11 HEK-293T C78(1.14)  LDD1583  [9]
 LDCM0380  CL110 HEK-293T C78(1.08)  LDD1584  [9]
 LDCM0381  CL111 HEK-293T C78(1.15)  LDD1585  [9]
 LDCM0382  CL112 HEK-293T C78(0.93)  LDD1586  [9]
 LDCM0384  CL114 HEK-293T C78(1.25)  LDD1588  [9]
 LDCM0385  CL115 HEK-293T C78(1.27)  LDD1589  [9]
 LDCM0386  CL116 HEK-293T C78(1.12)  LDD1590  [9]
 LDCM0388  CL118 HEK-293T C78(0.98)  LDD1592  [9]
 LDCM0389  CL119 HEK-293T C78(1.24)  LDD1593  [9]
 LDCM0390  CL12 HEK-293T C78(0.93)  LDD1594  [9]
 LDCM0391  CL120 HEK-293T C78(1.02)  LDD1595  [9]
 LDCM0393  CL122 HEK-293T C78(1.05)  LDD1597  [9]
 LDCM0394  CL123 HEK-293T C78(1.53)  LDD1598  [9]
 LDCM0395  CL124 HEK-293T C78(0.98)  LDD1599  [9]
 LDCM0397  CL126 HEK-293T C78(0.96)  LDD1601  [9]
 LDCM0398  CL127 HEK-293T C78(1.11)  LDD1602  [9]
 LDCM0399  CL128 HEK-293T C78(1.14)  LDD1603  [9]
 LDCM0401  CL14 HEK-293T C78(1.20)  LDD1605  [9]
 LDCM0402  CL15 HEK-293T C78(1.01)  LDD1606  [9]
 LDCM0403  CL16 HEK-293T C78(0.99)  LDD1607  [9]
 LDCM0404  CL17 HEK-293T C78(1.04)  LDD1608  [9]
 LDCM0405  CL18 HEK-293T C78(1.22)  LDD1609  [9]
 LDCM0406  CL19 HEK-293T C78(1.19)  LDD1610  [9]
 LDCM0407  CL2 HEK-293T C78(1.12)  LDD1611  [9]
 LDCM0408  CL20 HEK-293T C78(1.05)  LDD1612  [9]
 LDCM0409  CL21 HEK-293T C78(1.31)  LDD1613  [9]
 LDCM0410  CL22 HEK-293T C78(1.04)  LDD1614  [9]
 LDCM0411  CL23 HEK-293T C78(1.36)  LDD1615  [9]
 LDCM0412  CL24 HEK-293T C78(1.09)  LDD1616  [9]
 LDCM0414  CL26 HEK-293T C78(1.27)  LDD1618  [9]
 LDCM0415  CL27 HEK-293T C78(1.03)  LDD1619  [9]
 LDCM0416  CL28 HEK-293T C78(1.04)  LDD1620  [9]
 LDCM0417  CL29 HEK-293T C78(1.20)  LDD1621  [9]
 LDCM0418  CL3 HEK-293T C78(1.14)  LDD1622  [9]
 LDCM0419  CL30 HEK-293T C78(0.95)  LDD1623  [9]
 LDCM0420  CL31 HEK-293T C78(1.06)  LDD1624  [9]
 LDCM0421  CL32 HEK-293T C78(1.13)  LDD1625  [9]
 LDCM0422  CL33 HEK-293T C78(0.98)  LDD1626  [9]
 LDCM0423  CL34 HEK-293T C78(0.93)  LDD1627  [9]
 LDCM0424  CL35 HEK-293T C78(1.30)  LDD1628  [9]
 LDCM0425  CL36 HEK-293T C78(1.09)  LDD1629  [9]
 LDCM0428  CL39 HEK-293T C78(1.57)  LDD1632  [9]
 LDCM0429  CL4 HEK-293T C78(0.98)  LDD1633  [9]
 LDCM0430  CL40 HEK-293T C78(1.17)  LDD1634  [9]
 LDCM0431  CL41 HEK-293T C78(1.25)  LDD1635  [9]
 LDCM0432  CL42 HEK-293T C78(1.12)  LDD1636  [9]
 LDCM0433  CL43 HEK-293T C78(1.08)  LDD1637  [9]
 LDCM0434  CL44 HEK-293T C78(0.98)  LDD1638  [9]
 LDCM0435  CL45 HEK-293T C78(1.45)  LDD1639  [9]
 LDCM0436  CL46 HEK-293T C78(1.03)  LDD1640  [9]
 LDCM0437  CL47 HEK-293T C78(1.35)  LDD1641  [9]
 LDCM0438  CL48 HEK-293T C78(1.12)  LDD1642  [9]
 LDCM0440  CL5 HEK-293T C78(1.25)  LDD1644  [9]
 LDCM0441  CL50 HEK-293T C78(1.19)  LDD1645  [9]
 LDCM0443  CL52 HEK-293T C78(0.94)  LDD1646  [9]
 LDCM0444  CL53 HEK-293T C78(1.17)  LDD1647  [9]
 LDCM0445  CL54 HEK-293T C78(1.26)  LDD1648  [9]
 LDCM0446  CL55 HEK-293T C78(1.11)  LDD1649  [9]
 LDCM0447  CL56 HEK-293T C78(1.08)  LDD1650  [9]
 LDCM0448  CL57 HEK-293T C78(1.03)  LDD1651  [9]
 LDCM0449  CL58 HEK-293T C78(1.12)  LDD1652  [9]
 LDCM0450  CL59 HEK-293T C78(1.51)  LDD1653  [9]
 LDCM0451  CL6 HEK-293T C78(1.11)  LDD1654  [9]
 LDCM0452  CL60 HEK-293T C78(1.03)  LDD1655  [9]
 LDCM0454  CL62 HEK-293T C78(1.26)  LDD1657  [9]
 LDCM0455  CL63 HEK-293T C78(1.21)  LDD1658  [9]
 LDCM0456  CL64 HEK-293T C78(0.97)  LDD1659  [9]
 LDCM0457  CL65 HEK-293T C78(1.26)  LDD1660  [9]
 LDCM0458  CL66 HEK-293T C78(1.32)  LDD1661  [9]
 LDCM0459  CL67 HEK-293T C78(0.94)  LDD1662  [9]
 LDCM0460  CL68 HEK-293T C78(1.13)  LDD1663  [9]
 LDCM0461  CL69 HEK-293T C78(0.99)  LDD1664  [9]
 LDCM0462  CL7 HEK-293T C78(1.06)  LDD1665  [9]
 LDCM0463  CL70 HEK-293T C78(1.09)  LDD1666  [9]
 LDCM0464  CL71 HEK-293T C78(1.30)  LDD1667  [9]
 LDCM0465  CL72 HEK-293T C78(1.05)  LDD1668  [9]
 LDCM0467  CL74 HEK-293T C78(1.15)  LDD1670  [9]
 LDCM0469  CL76 HEK-293T C78(1.01)  LDD1672  [9]
 LDCM0470  CL77 HEK-293T C78(1.10)  LDD1673  [9]
 LDCM0471  CL78 HEK-293T C78(1.50)  LDD1674  [9]
 LDCM0472  CL79 HEK-293T C78(1.08)  LDD1675  [9]
 LDCM0473  CL8 HEK-293T C78(1.00)  LDD1676  [9]
 LDCM0474  CL80 HEK-293T C78(1.12)  LDD1677  [9]
 LDCM0475  CL81 HEK-293T C78(1.29)  LDD1678  [9]
 LDCM0476  CL82 HEK-293T C78(1.02)  LDD1679  [9]
 LDCM0477  CL83 HEK-293T C78(1.07)  LDD1680  [9]
 LDCM0478  CL84 HEK-293T C78(0.94)  LDD1681  [9]
 LDCM0480  CL86 HEK-293T C78(1.00)  LDD1683  [9]
 LDCM0481  CL87 HEK-293T C78(0.91)  LDD1684  [9]
 LDCM0482  CL88 HEK-293T C78(0.96)  LDD1685  [9]
 LDCM0483  CL89 HEK-293T C78(1.10)  LDD1686  [9]
 LDCM0484  CL9 HEK-293T C78(1.10)  LDD1687  [9]
 LDCM0485  CL90 HEK-293T C78(0.89)  LDD1688  [9]
 LDCM0486  CL91 HEK-293T C78(1.11)  LDD1689  [9]
 LDCM0487  CL92 HEK-293T C78(1.16)  LDD1690  [9]
 LDCM0488  CL93 HEK-293T C78(1.59)  LDD1691  [9]
 LDCM0489  CL94 HEK-293T C78(1.12)  LDD1692  [9]
 LDCM0490  CL95 HEK-293T C78(1.28)  LDD1693  [9]
 LDCM0491  CL96 HEK-293T C78(1.05)  LDD1694  [9]
 LDCM0493  CL98 HEK-293T C78(1.18)  LDD1696  [9]
 LDCM0494  CL99 HEK-293T C78(0.88)  LDD1697  [9]
 LDCM0495  E2913 HEK-293T C78(1.25)  LDD1698  [9]
 LDCM0625  F8 Ramos C78(1.48)  LDD2187  [11]
 LDCM0572  Fragment10 Ramos C78(0.81)  LDD2189  [11]
 LDCM0573  Fragment11 Ramos C78(0.39)  LDD2190  [11]
 LDCM0574  Fragment12 Ramos C78(0.87)  LDD2191  [11]
 LDCM0575  Fragment13 Ramos C78(1.39)  LDD2192  [11]
 LDCM0576  Fragment14 Ramos C78(0.93)  LDD2193  [11]
 LDCM0579  Fragment20 Ramos C78(0.70)  LDD2194  [11]
 LDCM0580  Fragment21 Ramos C78(1.68)  LDD2195  [11]
 LDCM0582  Fragment23 Ramos C78(1.31)  LDD2196  [11]
 LDCM0578  Fragment27 Ramos C78(0.99)  LDD2197  [11]
 LDCM0586  Fragment28 Ramos C78(0.90)  LDD2198  [11]
 LDCM0588  Fragment30 Ramos C78(1.48)  LDD2199  [11]
 LDCM0589  Fragment31 Ramos C78(1.06)  LDD2200  [11]
 LDCM0590  Fragment32 Ramos C78(0.76)  LDD2201  [11]
 LDCM0468  Fragment33 HEK-293T C78(0.94)  LDD1671  [9]
 LDCM0596  Fragment38 Ramos C78(1.29)  LDD2203  [11]
 LDCM0566  Fragment4 Ramos C78(0.85)  LDD2184  [11]
 LDCM0427  Fragment51 HEK-293T C78(0.98)  LDD1631  [9]
 LDCM0610  Fragment52 Ramos C78(1.09)  LDD2204  [11]
 LDCM0614  Fragment56 Ramos C78(0.63)  LDD2205  [11]
 LDCM0569  Fragment7 Ramos C78(0.95)  LDD2186  [11]
 LDCM0571  Fragment9 Ramos C78(0.59)  LDD2188  [11]
 LDCM0022  KB02 HEK-293T C78(1.00)  LDD1492  [9]
 LDCM0023  KB03 HEK-293T C78(0.97)  LDD1497  [9]
 LDCM0024  KB05 HMCB C41(1.03)  LDD3312  [2]
 LDCM0131  RA190 MM1.R C78(1.22)  LDD0304  [12]
 LDCM0021  THZ1 HCT 116 C41(1.25)  LDD2173  [10]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Probable E3 ubiquitin-protein ligase TRIML2 (TRIML2) . Q8N7C3
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
Gelsolin (GSN) Villin/gelsolin family P06396

References

1 Quantitative and Site-Specific Chemoproteomic Profiling of Targets of Acrolein. Chem Res Toxicol. 2019 Mar 18;32(3):467-473. doi: 10.1021/acs.chemrestox.8b00343. Epub 2019 Jan 15.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
3 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
4 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
5 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
6 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
7 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
8 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
9 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
10 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
11 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
12 Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190. Cell Chem Biol. 2020 Nov 19;27(11):1371-1382.e6. doi: 10.1016/j.chembiol.2020.08.007. Epub 2020 Aug 27.