Details of the Target

General Information of Target

Target ID LDTP12849
Target Name NF-kappa-B inhibitor-interacting Ras-like protein 1 (NKIRAS1)
Gene Name NKIRAS1
Gene ID 28512
Synonyms
KBRAS1; NF-kappa-B inhibitor-interacting Ras-like protein 1; I-kappa-B-interacting Ras-like protein 1; Kappa B-Ras protein 1; KappaB-Ras1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MGKSCKVVVCGQASVGKTSILEQLLYGNHVVGSEMIETQEDIYVGSIETDRGVREQVRFY
DTRGLRDGAELPRHCFSCTDGYVLVYSTDSRESFQRVELLKKEIDKSKDKKEVTIVVLGN
KCDLQEQRRVDPDVAQHWAKSEKVKLWEVSVADRRSLLEPFVYLASKMTQPQSKSAFPLS
RKNKGSGSLDG
Target Bioclass
Enzyme
Family
Small GTPase superfamily, Ras family, KappaB-Ras subfamily
Subcellular location
Cytoplasm
Function
Atypical Ras-like protein that acts as a potent regulator of NF-kappa-B activity by preventing the degradation of NF-kappa-B inhibitor beta (NFKBIB) by most signals, explaining why NFKBIB is more resistant to degradation. May act by blocking phosphorylation of NFKBIB and mediating cytoplasmic retention of p65/RELA NF-kappa-B subunit. It is unclear whether it acts as a GTPase. Both GTP- and GDP-bound forms block phosphorylation of NFKBIB.
Uniprot ID
Q9NYS0
Ensemble ID
ENST00000388759.7
HGNC ID
HGNC:17899

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C10(1.00)  LDD0547  [1]
IPM
 Probe Info  		N.A.  LDD2156  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C10(0.96)  LDD1507  [3]
 LDCM0215  AC10 HEK-293T C10(1.15)  LDD1508  [3]
 LDCM0226  AC11 HEK-293T C10(0.91)  LDD1509  [3]
 LDCM0230  AC113 HCT 116 C10(1.00)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C10(1.06)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C10(1.10)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C10(0.89)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C10(1.30)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C10(1.02)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C10(1.10)  LDD0553  [1]
 LDCM0238  AC120 HCT 116 C10(0.80)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C10(1.51)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C10(1.09)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C10(2.29)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C10(1.18)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C10(0.82)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C10(0.89)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C10(1.08)  LDD0562  [1]
 LDCM0259  AC14 HEK-293T C10(1.00)  LDD1512  [3]
 LDCM0270  AC15 HEK-293T C10(1.12)  LDD1513  [3]
 LDCM0276  AC17 HCT 116 C10(1.09)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C10(0.94)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C10(1.05)  LDD0595  [1]
 LDCM0279  AC2 HEK-293T C10(0.91)  LDD1518  [3]
 LDCM0280  AC20 HCT 116 C10(0.95)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C10(1.01)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C10(1.00)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C10(0.94)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C10(1.11)  LDD0601  [1]
 LDCM0285  AC25 HEK-293T C10(0.83)  LDD1524  [3]
 LDCM0286  AC26 HEK-293T C10(1.01)  LDD1525  [3]
 LDCM0287  AC27 HEK-293T C10(0.84)  LDD1526  [3]
 LDCM0290  AC3 HEK-293T C10(1.02)  LDD1529  [3]
 LDCM0291  AC30 HEK-293T C10(1.10)  LDD1530  [3]
 LDCM0292  AC31 HEK-293T C10(1.07)  LDD1531  [3]
 LDCM0294  AC33 HEK-293T C10(0.85)  LDD1533  [3]
 LDCM0295  AC34 HEK-293T C10(1.25)  LDD1534  [3]
 LDCM0296  AC35 HEK-293T C10(1.12)  LDD1535  [3]
 LDCM0299  AC38 HEK-293T C10(0.96)  LDD1538  [3]
 LDCM0300  AC39 HEK-293T C10(1.07)  LDD1539  [3]
 LDCM0303  AC41 HEK-293T C10(0.97)  LDD1542  [3]
 LDCM0304  AC42 HEK-293T C10(1.02)  LDD1543  [3]
 LDCM0305  AC43 HEK-293T C10(1.01)  LDD1544  [3]
 LDCM0308  AC46 HEK-293T C10(0.91)  LDD1547  [3]
 LDCM0309  AC47 HEK-293T C10(1.04)  LDD1548  [3]
 LDCM0311  AC49 HEK-293T C10(0.94)  LDD1550  [3]
 LDCM0313  AC50 HEK-293T C10(1.25)  LDD1552  [3]
 LDCM0314  AC51 HEK-293T C10(0.99)  LDD1553  [3]
 LDCM0317  AC54 HEK-293T C10(0.91)  LDD1556  [3]
 LDCM0318  AC55 HEK-293T C10(0.92)  LDD1557  [3]
 LDCM0320  AC57 HEK-293T C10(0.99)  LDD1559  [3]
 LDCM0321  AC58 HEK-293T C10(0.94)  LDD1560  [3]
 LDCM0322  AC59 HEK-293T C10(1.14)  LDD1561  [3]
 LDCM0323  AC6 HEK-293T C10(1.19)  LDD1562  [3]
 LDCM0326  AC62 HEK-293T C10(1.15)  LDD1565  [3]
 LDCM0327  AC63 HEK-293T C10(1.03)  LDD1566  [3]
 LDCM0334  AC7 HEK-293T C10(1.04)  LDD1568  [3]
 LDCM0356  AKOS034007680 HEK-293T C10(0.75)  LDD1570  [3]
 LDCM0368  CL10 HEK-293T C10(1.00)  LDD1572  [3]
 LDCM0369  CL100 HEK-293T C10(1.12)  LDD1573  [3]
 LDCM0371  CL102 HEK-293T C10(0.88)  LDD1575  [3]
 LDCM0372  CL103 HEK-293T C10(1.07)  LDD1576  [3]
 LDCM0373  CL104 HEK-293T C10(0.96)  LDD1577  [3]
 LDCM0374  CL105 HCT 116 C10(0.89)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C10(0.83)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C10(0.89)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C10(0.95)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C10(0.96)  LDD0695  [1]
 LDCM0379  CL11 HEK-293T C10(1.21)  LDD1583  [3]
 LDCM0380  CL110 HCT 116 C10(1.02)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C10(1.21)  LDD0698  [1]
 LDCM0382  CL112 HEK-293T C10(0.81)  LDD1586  [3]
 LDCM0384  CL114 HEK-293T C10(0.93)  LDD1588  [3]
 LDCM0385  CL115 HEK-293T C10(1.16)  LDD1589  [3]
 LDCM0386  CL116 HEK-293T C10(1.08)  LDD1590  [3]
 LDCM0388  CL118 HEK-293T C10(1.08)  LDD1592  [3]
 LDCM0389  CL119 HEK-293T C10(1.15)  LDD1593  [3]
 LDCM0391  CL120 HEK-293T C10(0.88)  LDD1595  [3]
 LDCM0393  CL122 HEK-293T C10(0.80)  LDD1597  [3]
 LDCM0394  CL123 HEK-293T C10(0.82)  LDD1598  [3]
 LDCM0395  CL124 HEK-293T C10(1.05)  LDD1599  [3]
 LDCM0397  CL126 HEK-293T C10(1.00)  LDD1601  [3]
 LDCM0398  CL127 HEK-293T C10(1.09)  LDD1602  [3]
 LDCM0399  CL128 HEK-293T C10(0.87)  LDD1603  [3]
 LDCM0401  CL14 HEK-293T C10(1.04)  LDD1605  [3]
 LDCM0402  CL15 HEK-293T C10(1.12)  LDD1606  [3]
 LDCM0403  CL16 HEK-293T C10(0.98)  LDD1607  [3]
 LDCM0404  CL17 HEK-293T C10(0.89)  LDD1608  [3]
 LDCM0405  CL18 HEK-293T C10(0.84)  LDD1609  [3]
 LDCM0406  CL19 HEK-293T C10(1.17)  LDD1610  [3]
 LDCM0407  CL2 HEK-293T C10(1.14)  LDD1611  [3]
 LDCM0410  CL22 HEK-293T C10(1.10)  LDD1614  [3]
 LDCM0411  CL23 HEK-293T C10(1.01)  LDD1615  [3]
 LDCM0414  CL26 HEK-293T C10(0.73)  LDD1618  [3]
 LDCM0415  CL27 HEK-293T C10(1.04)  LDD1619  [3]
 LDCM0416  CL28 HEK-293T C10(0.89)  LDD1620  [3]
 LDCM0417  CL29 HEK-293T C10(1.05)  LDD1621  [3]
 LDCM0418  CL3 HEK-293T C10(0.82)  LDD1622  [3]
 LDCM0419  CL30 HEK-293T C10(1.07)  LDD1623  [3]
 LDCM0420  CL31 HCT 116 C10(0.88)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C10(1.01)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C10(0.91)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C10(1.11)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C10(1.10)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C10(1.04)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C10(0.95)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C10(0.93)  LDD0745  [1]
 LDCM0429  CL4 HEK-293T C10(0.79)  LDD1633  [3]
 LDCM0430  CL40 HCT 116 C10(0.81)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C10(0.96)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C10(0.86)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C10(1.00)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C10(1.03)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C10(0.91)  LDD0752  [1]
 LDCM0436  CL46 HEK-293T C10(0.97)  LDD1640  [3]
 LDCM0437  CL47 HEK-293T C10(1.03)  LDD1641  [3]
 LDCM0440  CL5 HEK-293T C10(0.93)  LDD1644  [3]
 LDCM0441  CL50 HEK-293T C10(0.83)  LDD1645  [3]
 LDCM0443  CL52 HEK-293T C10(0.92)  LDD1646  [3]
 LDCM0444  CL53 HEK-293T C10(1.06)  LDD1647  [3]
 LDCM0445  CL54 HEK-293T C10(1.04)  LDD1648  [3]
 LDCM0446  CL55 HEK-293T C10(1.14)  LDD1649  [3]
 LDCM0449  CL58 HEK-293T C10(1.14)  LDD1652  [3]
 LDCM0450  CL59 HEK-293T C10(1.03)  LDD1653  [3]
 LDCM0451  CL6 HEK-293T C10(0.81)  LDD1654  [3]
 LDCM0453  CL61 HCT 116 C10(1.02)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C10(1.03)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C10(1.02)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C10(1.11)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C10(0.99)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C10(1.05)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C10(1.01)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C10(0.89)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C10(1.03)  LDD0778  [1]
 LDCM0462  CL7 HEK-293T C10(1.04)  LDD1665  [3]
 LDCM0463  CL70 HCT 116 C10(0.97)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C10(1.07)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C10(1.06)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C10(0.94)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C10(0.97)  LDD0784  [1]
 LDCM0469  CL76 HEK-293T C10(0.90)  LDD1672  [3]
 LDCM0470  CL77 HEK-293T C10(1.02)  LDD1673  [3]
 LDCM0471  CL78 HEK-293T C10(1.14)  LDD1674  [3]
 LDCM0472  CL79 HEK-293T C10(0.88)  LDD1675  [3]
 LDCM0476  CL82 HEK-293T C10(0.95)  LDD1679  [3]
 LDCM0477  CL83 HEK-293T C10(1.35)  LDD1680  [3]
 LDCM0480  CL86 HEK-293T C10(1.07)  LDD1683  [3]
 LDCM0481  CL87 HEK-293T C10(1.17)  LDD1684  [3]
 LDCM0482  CL88 HEK-293T C10(1.51)  LDD1685  [3]
 LDCM0483  CL89 HEK-293T C10(1.02)  LDD1686  [3]
 LDCM0485  CL90 HEK-293T C10(1.17)  LDD1688  [3]
 LDCM0486  CL91 HEK-293T C10(1.21)  LDD1689  [3]
 LDCM0489  CL94 HEK-293T C10(1.08)  LDD1692  [3]
 LDCM0490  CL95 HEK-293T C10(0.89)  LDD1693  [3]
 LDCM0493  CL98 HEK-293T C10(0.91)  LDD1696  [3]
 LDCM0494  CL99 HEK-293T C10(1.13)  LDD1697  [3]
 LDCM0495  E2913 HEK-293T C10(1.16)  LDD1698  [3]
 LDCM0468  Fragment33 HCT 116 C10(1.02)  LDD0785  [1]
 LDCM0427  Fragment51 HCT 116 C10(0.91)  LDD0744  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
3 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402