Details of the Target

General Information of Target

Target ID LDTP11108
Target Name Vacuolar protein-sorting-associated protein 25 (VPS25)
Gene Name VPS25
Gene ID 84313
Synonyms
DERP9; EAP20; Vacuolar protein-sorting-associated protein 25; hVps25; Dermal papilla-derived protein 9; ELL-associated protein of 20 kDa; ESCRT-II complex subunit VPS25
3D Structure
Download
2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MSAAEAGGVFHRARGRTLAAFPAEKESEWKGPFYFILGADPQFGLIKAWSTGDCDNGGDE
WEQEIRLTEQAVQAINKLNPKPKFFVLCGDLIHAMPGKPWRTEQTEDLKRVLRAVDRAIP
LVLVSGNHDIGNTPTAETVEEFCRTWGDDYFSFWVGGVLFLVLNSQFYENPSKCPSLKQA
QDQWLDEQLSIARQRHCQHAIVFQHIPLFLESIDEDDDYYFNLSKSTRKKLADKFIHAGV
KVVFSGHYHRNAGGTYQNLDMVVSSAIGCQLGRDPHGLRVVVVTAEKIVHRYYSLDELSE
KGIEDDLMDLIKKK
Target Bioclass
Other
Family
VPS25 family
Subcellular location
Cytoplasm
Function
Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. The ESCRT-II complex may be involved in facilitating the budding of certain RNA viruses.
Uniprot ID
Q9BRG1
Ensemble ID
ENST00000253794.7
HGNC ID
HGNC:28122

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 7 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K68(10.00); K84(3.31)  LDD0277  [1]
Acrolein
 Probe Info  		N.A.  LDD0221  [2]
DBIA
 Probe Info  		C34(1.37); C41(1.37)  LDD1109  [3]
5E-2FA
 Probe Info  		N.A.  LDD2235  [4]
m-APA
 Probe Info  		N.A.  LDD2231  [4]
IA-alkyne
 Probe Info  		C41(0.00); C34(0.00)  LDD0036  [5]
AOyne
 Probe Info  		15.00  LDD0443  [6]
PAL-AfBPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C170
 Probe Info  		8.06  LDD1850  [7]
C278
 Probe Info  		72.50  LDD1948  [7]
C280
 Probe Info  		12.13  LDD1950  [7]
C282
 Probe Info  		16.56  LDD1952  [7]
C284
 Probe Info  		27.86  LDD1954  [7]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0230  AC113 PaTu 8988t C34(1.37); C41(1.37)  LDD1109  [3]
 LDCM0231  AC114 PaTu 8988t C34(0.79); C41(0.79)  LDD1110  [3]
 LDCM0232  AC115 PaTu 8988t C34(1.26); C41(1.26)  LDD1111  [3]
 LDCM0233  AC116 PaTu 8988t C34(1.33); C41(1.33)  LDD1112  [3]
 LDCM0234  AC117 PaTu 8988t C34(1.09); C41(1.09)  LDD1113  [3]
 LDCM0235  AC118 PaTu 8988t C34(1.00); C41(1.00)  LDD1114  [3]
 LDCM0236  AC119 PaTu 8988t C34(0.93); C41(0.93)  LDD1115  [3]
 LDCM0238  AC120 PaTu 8988t C34(0.83); C41(0.83)  LDD1117  [3]
 LDCM0239  AC121 PaTu 8988t C34(0.71); C41(0.71)  LDD1118  [3]
 LDCM0240  AC122 PaTu 8988t C34(1.06); C41(1.06)  LDD1119  [3]
 LDCM0241  AC123 PaTu 8988t C34(1.30); C41(1.30)  LDD1120  [3]
 LDCM0242  AC124 PaTu 8988t C34(0.81); C41(0.81)  LDD1121  [3]
 LDCM0243  AC125 PaTu 8988t C34(1.02); C41(1.02)  LDD1122  [3]
 LDCM0244  AC126 PaTu 8988t C34(1.16); C41(1.16)  LDD1123  [3]
 LDCM0245  AC127 PaTu 8988t C34(0.62); C41(0.62)  LDD1124  [3]
 LDCM0263  AC143 PaTu 8988t C34(0.87); C41(0.87)  LDD1142  [3]
 LDCM0264  AC144 PaTu 8988t C34(0.87); C41(0.87)  LDD1143  [3]
 LDCM0265  AC145 PaTu 8988t C34(1.07); C41(1.07)  LDD1144  [3]
 LDCM0266  AC146 PaTu 8988t C34(0.76); C41(0.76)  LDD1145  [3]
 LDCM0267  AC147 PaTu 8988t C34(1.04); C41(1.04)  LDD1146  [3]
 LDCM0268  AC148 PaTu 8988t C34(0.62); C41(0.62)  LDD1147  [3]
 LDCM0269  AC149 PaTu 8988t C34(0.52); C41(0.52)  LDD1148  [3]
 LDCM0270  AC15 HEK-293T C41(1.46)  LDD1513  [8]
 LDCM0271  AC150 PaTu 8988t C34(0.49); C41(0.49)  LDD1150  [3]
 LDCM0272  AC151 PaTu 8988t C34(0.50); C41(0.50)  LDD1151  [3]
 LDCM0273  AC152 PaTu 8988t C34(0.52); C41(0.52)  LDD1152  [3]
 LDCM0274  AC153 PaTu 8988t C34(0.42); C41(0.42)  LDD1153  [3]
 LDCM0621  AC154 PaTu 8988t C34(0.40); C41(0.40)  LDD2166  [3]
 LDCM0622  AC155 PaTu 8988t C34(0.51); C41(0.51)  LDD2167  [3]
 LDCM0623  AC156 PaTu 8988t C34(0.41); C41(0.41)  LDD2168  [3]
 LDCM0624  AC157 PaTu 8988t C34(0.50); C41(0.50)  LDD2169  [3]
 LDCM0276  AC17 PaTu 8988t C34(0.77); C41(0.77)  LDD1155  [3]
 LDCM0277  AC18 PaTu 8988t C34(0.43); C41(0.43)  LDD1156  [3]
 LDCM0278  AC19 PaTu 8988t C34(0.66); C41(0.66)  LDD1157  [3]
 LDCM0280  AC20 PaTu 8988t C34(0.62); C41(0.62)  LDD1159  [3]
 LDCM0281  AC21 PaTu 8988t C34(0.40); C41(0.40)  LDD1160  [3]
 LDCM0282  AC22 PaTu 8988t C34(0.46); C41(0.46)  LDD1161  [3]
 LDCM0283  AC23 PaTu 8988t C34(0.53); C41(0.53)  LDD1162  [3]
 LDCM0284  AC24 PaTu 8988t C34(0.66); C41(0.66)  LDD1163  [3]
 LDCM0285  AC25 PaTu 8988t C34(1.77); C41(1.77)  LDD1164  [3]
 LDCM0286  AC26 PaTu 8988t C34(0.83); C41(0.83)  LDD1165  [3]
 LDCM0287  AC27 PaTu 8988t C34(0.97); C41(0.97)  LDD1166  [3]
 LDCM0288  AC28 PaTu 8988t C34(0.92); C41(0.92)  LDD1167  [3]
 LDCM0289  AC29 PaTu 8988t C34(1.08); C41(1.08)  LDD1168  [3]
 LDCM0291  AC30 PaTu 8988t C34(1.11); C41(1.11)  LDD1170  [3]
 LDCM0292  AC31 PaTu 8988t C34(1.74); C41(1.74)  LDD1171  [3]
 LDCM0293  AC32 PaTu 8988t C34(1.61); C41(1.61)  LDD1172  [3]
 LDCM0294  AC33 PaTu 8988t C34(0.92); C41(0.92)  LDD1173  [3]
 LDCM0295  AC34 PaTu 8988t C34(0.84); C41(0.84)  LDD1174  [3]
 LDCM0300  AC39 HEK-293T C41(1.13)  LDD1539  [8]
 LDCM0309  AC47 HEK-293T C41(1.27)  LDD1548  [8]
 LDCM0318  AC55 HEK-293T C41(1.11)  LDD1557  [8]
 LDCM0320  AC57 PaTu 8988t C41(0.68)  LDD1199  [3]
 LDCM0321  AC58 PaTu 8988t C41(0.57)  LDD1200  [3]
 LDCM0322  AC59 PaTu 8988t C41(0.78)  LDD1201  [3]
 LDCM0324  AC60 PaTu 8988t C41(0.59)  LDD1203  [3]
 LDCM0325  AC61 PaTu 8988t C41(0.74)  LDD1204  [3]
 LDCM0326  AC62 PaTu 8988t C41(0.75)  LDD1205  [3]
 LDCM0327  AC63 PaTu 8988t C41(0.68)  LDD1206  [3]
 LDCM0328  AC64 PaTu 8988t C41(0.93)  LDD1207  [3]
 LDCM0329  AC65 PaTu 8988t C41(0.78)  LDD1208  [3]
 LDCM0330  AC66 PaTu 8988t C41(0.74)  LDD1209  [3]
 LDCM0331  AC67 PaTu 8988t C41(1.04)  LDD1210  [3]
 LDCM0334  AC7 HEK-293T C41(1.18)  LDD1568  [8]
 LDCM0367  CL1 PaTu 8988t C34(1.17); C41(1.17)  LDD1246  [3]
 LDCM0368  CL10 PaTu 8988t C34(0.50); C41(0.50)  LDD1247  [3]
 LDCM0374  CL105 PaTu 8988t C34(0.76); C41(0.76)  LDD1253  [3]
 LDCM0375  CL106 PaTu 8988t C34(0.77); C41(0.77)  LDD1254  [3]
 LDCM0376  CL107 PaTu 8988t C34(0.73); C41(0.73)  LDD1255  [3]
 LDCM0377  CL108 PaTu 8988t C34(0.86); C41(0.86)  LDD1256  [3]
 LDCM0378  CL109 PaTu 8988t C34(0.57); C41(0.57)  LDD1257  [3]
 LDCM0379  CL11 PaTu 8988t C34(1.06); C41(1.06)  LDD1258  [3]
 LDCM0380  CL110 PaTu 8988t C34(0.58); C41(0.58)  LDD1259  [3]
 LDCM0381  CL111 PaTu 8988t C34(0.44); C41(0.44)  LDD1260  [3]
 LDCM0382  CL112 PaTu 8988t C34(1.21); C41(1.21)  LDD1261  [3]
 LDCM0383  CL113 PaTu 8988t C34(1.09); C41(1.09)  LDD1262  [3]
 LDCM0384  CL114 PaTu 8988t C34(1.07); C41(1.07)  LDD1263  [3]
 LDCM0385  CL115 PaTu 8988t C34(1.27); C41(1.27)  LDD1264  [3]
 LDCM0386  CL116 PaTu 8988t C34(0.92); C41(0.92)  LDD1265  [3]
 LDCM0390  CL12 PaTu 8988t C34(0.91); C41(0.91)  LDD1269  [3]
 LDCM0396  CL125 PaTu 8988t C41(0.76)  LDD1275  [3]
 LDCM0397  CL126 PaTu 8988t C41(0.68)  LDD1276  [3]
 LDCM0398  CL127 PaTu 8988t C41(0.97)  LDD1277  [3]
 LDCM0399  CL128 PaTu 8988t C41(0.79)  LDD1278  [3]
 LDCM0400  CL13 PaTu 8988t C34(0.54); C41(0.54)  LDD1279  [3]
 LDCM0401  CL14 PaTu 8988t C34(0.69); C41(0.69)  LDD1280  [3]
 LDCM0402  CL15 PaTu 8988t C34(0.83); C41(0.83)  LDD1281  [3]
 LDCM0403  CL16 PaTu 8988t C34(0.43); C41(0.43)  LDD1282  [3]
 LDCM0404  CL17 PaTu 8988t C34(0.41); C41(0.41)  LDD1283  [3]
 LDCM0405  CL18 PaTu 8988t C34(0.27); C41(0.27)  LDD1284  [3]
 LDCM0406  CL19 PaTu 8988t C34(0.34); C41(0.34)  LDD1285  [3]
 LDCM0407  CL2 PaTu 8988t C34(1.04); C41(1.04)  LDD1286  [3]
 LDCM0408  CL20 PaTu 8988t C34(0.39); C41(0.39)  LDD1287  [3]
 LDCM0409  CL21 PaTu 8988t C34(0.32); C41(0.32)  LDD1288  [3]
 LDCM0410  CL22 PaTu 8988t C34(0.28); C41(0.28)  LDD1289  [3]
 LDCM0411  CL23 PaTu 8988t C34(0.32); C41(0.32)  LDD1290  [3]
 LDCM0412  CL24 PaTu 8988t C34(0.25); C41(0.25)  LDD1291  [3]
 LDCM0413  CL25 PaTu 8988t C34(0.42); C41(0.42)  LDD1292  [3]
 LDCM0414  CL26 PaTu 8988t C34(0.37); C41(0.37)  LDD1293  [3]
 LDCM0415  CL27 PaTu 8988t C34(0.34); C41(0.34)  LDD1294  [3]
 LDCM0416  CL28 PaTu 8988t C34(0.32); C41(0.32)  LDD1295  [3]
 LDCM0417  CL29 PaTu 8988t C34(0.33); C41(0.33)  LDD1296  [3]
 LDCM0418  CL3 PaTu 8988t C34(0.67); C41(0.67)  LDD1297  [3]
 LDCM0419  CL30 PaTu 8988t C34(0.27); C41(0.27)  LDD1298  [3]
 LDCM0424  CL35 HEK-293T C41(0.80)  LDD1628  [8]
 LDCM0429  CL4 PaTu 8988t C34(0.79); C41(0.79)  LDD1308  [3]
 LDCM0437  CL47 HEK-293T C41(0.99)  LDD1641  [8]
 LDCM0440  CL5 PaTu 8988t C34(0.64); C41(0.64)  LDD1319  [3]
 LDCM0450  CL59 HEK-293T C41(0.90)  LDD1653  [8]
 LDCM0451  CL6 PaTu 8988t C34(0.63); C41(0.63)  LDD1330  [3]
 LDCM0462  CL7 PaTu 8988t C34(0.58); C41(0.58)  LDD1341  [3]
 LDCM0464  CL71 HEK-293T C41(0.95)  LDD1667  [8]
 LDCM0473  CL8 PaTu 8988t C34(0.56); C41(0.56)  LDD1352  [3]
 LDCM0477  CL83 HEK-293T C41(0.91)  LDD1680  [8]
 LDCM0484  CL9 PaTu 8988t C34(0.66); C41(0.66)  LDD1363  [3]
 LDCM0490  CL95 HEK-293T C41(0.91)  LDD1693  [8]
 LDCM0107  IAA HeLa N.A.  LDD0221  [2]
 LDCM0022  KB02 HEK-293T C34(0.85); C41(0.85)  LDD1492  [8]
 LDCM0023  KB03 HEK-293T C34(0.87); C41(0.87)  LDD1497  [8]
 LDCM0024  KB05 HEK-293T C34(1.18); C41(1.18)  LDD1502  [8]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 4 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Maspardin (SPG21) AB hydrolase superfamily Q9NZD8
60 kDa lysophospholipase (ASPG) Asparaginase 1 family Q86U10
Septin-14 (SEPTIN14) Septin GTPase family Q6ZU15
Zinc finger protein RFP (TRIM27) TRIM/RBCC family P14373
Transcription factor
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proto-oncogene c-Rel (REL) . Q04864
Transcriptional adapter 2-alpha (TADA2A) . O75478
Cytokine and receptor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Cytokine receptor-like factor 3 (CRLF3) Cytokine receptor-like factor 3 family Q8IUI8
Other
Click To Hide/Show 9 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
B9 domain-containing protein 2 (B9D2) B9D family Q9BPU9
BPI fold-containing family A member 1 (BPIFA1) Plunc family Q9NP55
Charged multivesicular body protein 6 (CHMP6) SNF7 family Q96FZ7
Vacuolar-sorting protein SNF8 (SNF8) SNF8 family Q96H20
Synaptonemal complex central element protein 1 (SYCE1) SYCE family Q8N0S2
Vacuolar protein-sorting-associated protein 36 (VPS36) VPS36 family Q86VN1
BEN domain-containing protein 5 (BEND5) . Q7L4P6
PRKCA-binding protein (PICK1) . Q9NRD5
Syntenin-2 (SDCBP2) . Q9H190

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
3 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
4 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
7 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587
8 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402