Details of the Target

General Information of Target

Target ID LDTP10766
Target Name Bardet-Biedl syndrome 4 protein (BBS4)
Gene Name BBS4
Gene ID 585
Synonyms
Bardet-Biedl syndrome 4 protein
3D Structure
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2D Sequence (FASTA)
Download
3D Structure (PDB)
Download
Sequence
MYSAHRPLMPASSAASRGLGMFVWTNVEPRSVAVFPWHSLVPFLAPSQPDPSVQPSEAQQ
PASHPVASNQSKEPAESAAVAHERPPGGTGSADPERPPGATCPESPGPGPPHPLGVVESG
KGPPPTTEEEASGPPGEPRLDSETESDHDDAFLSIMSPEIQLPLPPGKRRTQSLSALPKE
RDSSSEKDGRSPNKREKDHIRRPMNAFMIFSKRHRALVHQRHPNQDNRTVSKILGEWWYA
LGPKEKQKYHDLAFQVKEAHFKAHPDWKWCNKDRKKSSSEAKPTSLGLAGGHKETRERSM
SETGTAAAPGVSSELLSVAAQTLLSSDTKAPGSSSCGAERLHTVGGPGSARPRAFSHSGV
HSLDGGEVDSQALQELTQMVSGPASYSGPKPSTQYGAPGPFAAPGEGGALAATGRPPLLP
TRASRSQRAASEDMTSDEERMVICEEEGDDDVIADDGFGTTDIDLKCKERVTDSESGDSS
GEDPEGNKGFGRKVFSPVIRSSFTHCRPPLDPEPPGPPDPPVAFGKGYGSAPSSSASSPA
SSSASAATSFSLGSGTFKAQESGQGSTAGPLRPPPPGAGGPATPSKATRFLPMDPATFRR
KRPESVGGLEPPGPSVIAAPPSGGGNILQTLVLPPNKEEQEGGGARVPSAPAPSLAYGAP
AAPLSRPAATMVTNVVRPVSSTPVPIASKPFPTSGRAEASPNDTAGARTEMGTGSRVPGG
SPLGVSLVYSDKKSAAATSPAPHLVAGPLLGTVGKAPATVTNLLVGTPGYGAPAPPAVQF
IAQGAPGGGTTAGSGAGAGSGPNGPVPLGILQPGALGKAGGITQVQYILPTLPQQLQVAP
APAPAPGTKAAAPSGPAPTTSIRFTLPPGTSTNGKVLAATAPTPGIPILQSVPSAPPPKA
QSVSPVQAPPPGGSAQLLPGKVLVPLAAPSMSVRGGGAGQPLPLVSPPFSVPVQNGAQPP
SKIIQLTPVPVSTPSGLVPPLSPATLPGPTSQPQKVLLPSSTRITYVQSAGGHALPLGTS
PASSQAGTVTSYGPTSSVALGFTSLGPSGPAFVQPLLSAGQAPLLAPGQVGVSPVPSPQL
PPACAAPGGPVITAFYSGSPAPTSSAPLAQPSQAPPSLVYTVATSTTPPAATILPKGPPA
PATATPAPTSPFPSATAGSMTYSLVAPKAQRPSPKAPQKVKAAIASIPVGSFEAGASGRP
GPAPRQPLEPGPVREPTAPESELEGQPTPPAPPPLPETWTPTARSSPPLPPPAEERTSAK
GPETMASKFPSSSSDWRVPGQGLENRGEPPTPPSPAPAPAVAPGGSSESSSGRAAGDTPE
RKEAAGTGKKVKVRPPPLKKTFDSVDNRVLSEVDFEERFAELPEFRPEEVLPSPTLQSLA
TSPRAILGSYRKKRKNSTDLDSAPEDPTSPKRKMRRRSSCSSEPNTPKSAKCEGDIFTFD
RTGTEAEDVLGELEYDKVPYSSLRRTLDQRRALVMQLFQDHGFFPSAQATAAFQARYADI
FPSKVCLQLKIREVRQKIMQAATPTEQPPGAEAPLPVPPPTGTAAAPAPTPSPAGGPDPT
SPSSDSGTAQAAPPLPPPPESGPGQPGWEGAPQPSPPPPGPSTAATGR
Target Bioclass
Transporter and channel
Family
BBS4 family
Subcellular location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Function
The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for proper BBSome complex assembly and its ciliary localization. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome.
Uniprot ID
Q96RK4
Ensemble ID
ENST00000268057.9
HGNC ID
HGNC:969

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
SW948 SNV: p.I31N .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C370(0.95); C264(0.95)  LDD1508  [1]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [2]
TFBX
 Probe Info  		N.A.  LDD0148  [3]
AOyne
 Probe Info  		10.70  LDD0443  [4]
PAL-AfBPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
C056
 Probe Info  		16.68  LDD1753  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0215  AC10 HEK-293T C370(0.95); C264(0.95)  LDD1508  [1]
 LDCM0226  AC11 HEK-293T C370(1.00); C264(0.92)  LDD1509  [1]
 LDCM0237  AC12 HEK-293T C370(1.02); C264(0.99)  LDD1510  [1]
 LDCM0259  AC14 HEK-293T C370(1.10)  LDD1512  [1]
 LDCM0270  AC15 HEK-293T C370(1.11)  LDD1513  [1]
 LDCM0277  AC18 HEK-293T C370(0.69); C264(0.98)  LDD1516  [1]
 LDCM0278  AC19 HEK-293T C370(0.96); C264(0.62)  LDD1517  [1]
 LDCM0279  AC2 HEK-293T C370(1.15); C264(0.96)  LDD1518  [1]
 LDCM0280  AC20 HEK-293T C370(1.17); C264(0.89)  LDD1519  [1]
 LDCM0281  AC21 HEK-293T C370(1.10)  LDD1520  [1]
 LDCM0282  AC22 HEK-293T C370(0.88)  LDD1521  [1]
 LDCM0283  AC23 HEK-293T C370(1.12)  LDD1522  [1]
 LDCM0284  AC24 HEK-293T C370(1.04)  LDD1523  [1]
 LDCM0286  AC26 HEK-293T C370(0.93); C264(0.81)  LDD1525  [1]
 LDCM0287  AC27 HEK-293T C370(1.19); C264(1.21)  LDD1526  [1]
 LDCM0288  AC28 HEK-293T C370(1.15); C264(0.88)  LDD1527  [1]
 LDCM0289  AC29 HEK-293T C370(1.29)  LDD1528  [1]
 LDCM0290  AC3 HEK-293T C370(1.05); C264(0.74)  LDD1529  [1]
 LDCM0291  AC30 HEK-293T C370(0.97)  LDD1530  [1]
 LDCM0292  AC31 HEK-293T C370(0.97)  LDD1531  [1]
 LDCM0293  AC32 HEK-293T C370(1.03)  LDD1532  [1]
 LDCM0295  AC34 HEK-293T C370(0.75); C264(0.97)  LDD1534  [1]
 LDCM0296  AC35 HEK-293T C370(0.97); C264(0.84)  LDD1535  [1]
 LDCM0297  AC36 HEK-293T C370(1.13); C264(1.19)  LDD1536  [1]
 LDCM0298  AC37 HEK-293T C370(1.03)  LDD1537  [1]
 LDCM0299  AC38 HEK-293T C370(0.94)  LDD1538  [1]
 LDCM0300  AC39 HEK-293T C370(1.14)  LDD1539  [1]
 LDCM0301  AC4 HEK-293T C370(1.16); C264(1.24)  LDD1540  [1]
 LDCM0302  AC40 HEK-293T C370(0.90)  LDD1541  [1]
 LDCM0304  AC42 HEK-293T C370(1.02); C264(1.02)  LDD1543  [1]
 LDCM0305  AC43 HEK-293T C370(1.25); C264(0.78)  LDD1544  [1]
 LDCM0306  AC44 HEK-293T C370(1.10); C264(1.24)  LDD1545  [1]
 LDCM0307  AC45 HEK-293T C370(1.05)  LDD1546  [1]
 LDCM0308  AC46 HEK-293T C370(1.05)  LDD1547  [1]
 LDCM0309  AC47 HEK-293T C370(0.97)  LDD1548  [1]
 LDCM0310  AC48 HEK-293T C370(1.03)  LDD1549  [1]
 LDCM0312  AC5 HEK-293T C370(1.06)  LDD1551  [1]
 LDCM0313  AC50 HEK-293T C370(1.17); C264(0.96)  LDD1552  [1]
 LDCM0314  AC51 HEK-293T C370(1.33); C264(0.88)  LDD1553  [1]
 LDCM0315  AC52 HEK-293T C370(1.02); C264(0.94)  LDD1554  [1]
 LDCM0316  AC53 HEK-293T C370(1.18)  LDD1555  [1]
 LDCM0317  AC54 HEK-293T C370(0.93)  LDD1556  [1]
 LDCM0318  AC55 HEK-293T C370(0.98)  LDD1557  [1]
 LDCM0319  AC56 HEK-293T C370(1.06)  LDD1558  [1]
 LDCM0321  AC58 HEK-293T C370(1.04); C264(1.18)  LDD1560  [1]
 LDCM0322  AC59 HEK-293T C370(1.00); C264(0.91)  LDD1561  [1]
 LDCM0323  AC6 HEK-293T C370(0.90)  LDD1562  [1]
 LDCM0324  AC60 HEK-293T C370(0.96); C264(0.99)  LDD1563  [1]
 LDCM0325  AC61 HEK-293T C370(1.13)  LDD1564  [1]
 LDCM0326  AC62 HEK-293T C370(1.06)  LDD1565  [1]
 LDCM0327  AC63 HEK-293T C370(1.01)  LDD1566  [1]
 LDCM0328  AC64 HEK-293T C370(1.11)  LDD1567  [1]
 LDCM0334  AC7 HEK-293T C370(1.00)  LDD1568  [1]
 LDCM0345  AC8 HEK-293T C370(1.14)  LDD1569  [1]
 LDCM0248  AKOS034007472 HEK-293T C370(1.07)  LDD1511  [1]
 LDCM0275  AKOS034007705 HEK-293T C370(1.10)  LDD1514  [1]
 LDCM0367  CL1 HEK-293T C264(0.85)  LDD1571  [1]
 LDCM0368  CL10 HEK-293T C370(0.89)  LDD1572  [1]
 LDCM0370  CL101 HEK-293T C264(0.89)  LDD1574  [1]
 LDCM0371  CL102 HEK-293T C264(1.02)  LDD1575  [1]
 LDCM0372  CL103 HEK-293T C370(1.15); C264(0.97)  LDD1576  [1]
 LDCM0374  CL105 HEK-293T C264(0.79)  LDD1578  [1]
 LDCM0375  CL106 HEK-293T C264(0.91)  LDD1579  [1]
 LDCM0376  CL107 HEK-293T C370(1.13); C264(0.98)  LDD1580  [1]
 LDCM0378  CL109 HEK-293T C264(0.96)  LDD1582  [1]
 LDCM0379  CL11 HEK-293T C370(1.09)  LDD1583  [1]
 LDCM0380  CL110 HEK-293T C264(0.90)  LDD1584  [1]
 LDCM0381  CL111 HEK-293T C370(0.91); C264(0.98)  LDD1585  [1]
 LDCM0383  CL113 HEK-293T C264(0.82)  LDD1587  [1]
 LDCM0384  CL114 HEK-293T C264(0.92)  LDD1588  [1]
 LDCM0385  CL115 HEK-293T C370(0.89); C264(0.97)  LDD1589  [1]
 LDCM0387  CL117 HEK-293T C264(0.96)  LDD1591  [1]
 LDCM0388  CL118 HEK-293T C264(1.03)  LDD1592  [1]
 LDCM0389  CL119 HEK-293T C370(0.84); C264(1.01)  LDD1593  [1]
 LDCM0390  CL12 HEK-293T C370(1.06)  LDD1594  [1]
 LDCM0392  CL121 HEK-293T C264(0.92)  LDD1596  [1]
 LDCM0393  CL122 HEK-293T C264(0.98)  LDD1597  [1]
 LDCM0394  CL123 HEK-293T C370(0.94); C264(0.93)  LDD1598  [1]
 LDCM0396  CL125 HEK-293T C264(1.03)  LDD1600  [1]
 LDCM0397  CL126 HEK-293T C264(0.84)  LDD1601  [1]
 LDCM0398  CL127 HEK-293T C370(1.07); C264(1.13)  LDD1602  [1]
 LDCM0400  CL13 HEK-293T C264(0.84)  LDD1604  [1]
 LDCM0401  CL14 HEK-293T C264(0.99)  LDD1605  [1]
 LDCM0402  CL15 HEK-293T C370(1.00); C264(0.89)  LDD1606  [1]
 LDCM0405  CL18 HEK-293T C370(0.90); C264(1.06)  LDD1609  [1]
 LDCM0406  CL19 HEK-293T C370(1.09); C264(1.02)  LDD1610  [1]
 LDCM0407  CL2 HEK-293T C264(0.88)  LDD1611  [1]
 LDCM0408  CL20 HEK-293T C370(1.06); C264(1.15)  LDD1612  [1]
 LDCM0409  CL21 HEK-293T C370(1.14)  LDD1613  [1]
 LDCM0410  CL22 HEK-293T C370(1.02)  LDD1614  [1]
 LDCM0411  CL23 HEK-293T C370(1.08)  LDD1615  [1]
 LDCM0412  CL24 HEK-293T C370(0.94)  LDD1616  [1]
 LDCM0413  CL25 HEK-293T C264(0.86)  LDD1617  [1]
 LDCM0414  CL26 HEK-293T C264(0.96)  LDD1618  [1]
 LDCM0415  CL27 HEK-293T C370(0.84); C264(1.02)  LDD1619  [1]
 LDCM0418  CL3 HEK-293T C370(0.89); C264(1.06)  LDD1622  [1]
 LDCM0419  CL30 HEK-293T C370(1.11); C264(0.81)  LDD1623  [1]
 LDCM0420  CL31 HEK-293T C370(1.06); C264(0.95)  LDD1624  [1]
 LDCM0421  CL32 HEK-293T C370(1.13); C264(0.92)  LDD1625  [1]
 LDCM0422  CL33 HEK-293T C370(0.62)  LDD1626  [1]
 LDCM0423  CL34 HEK-293T C370(0.70)  LDD1627  [1]
 LDCM0424  CL35 HEK-293T C370(0.97)  LDD1628  [1]
 LDCM0425  CL36 HEK-293T C370(0.90)  LDD1629  [1]
 LDCM0426  CL37 HEK-293T C264(0.85)  LDD1630  [1]
 LDCM0428  CL39 HEK-293T C370(0.86); C264(0.94)  LDD1632  [1]
 LDCM0432  CL42 HEK-293T C370(1.04); C264(1.01)  LDD1636  [1]
 LDCM0433  CL43 HEK-293T C370(0.98); C264(1.40)  LDD1637  [1]
 LDCM0434  CL44 HEK-293T C370(1.14); C264(1.02)  LDD1638  [1]
 LDCM0435  CL45 HEK-293T C370(0.91)  LDD1639  [1]
 LDCM0436  CL46 HEK-293T C370(0.86)  LDD1640  [1]
 LDCM0437  CL47 HEK-293T C370(1.02)  LDD1641  [1]
 LDCM0438  CL48 HEK-293T C370(0.95)  LDD1642  [1]
 LDCM0439  CL49 HEK-293T C264(1.02)  LDD1643  [1]
 LDCM0441  CL50 HEK-293T C264(1.02)  LDD1645  [1]
 LDCM0445  CL54 HEK-293T C370(0.74); C264(0.81)  LDD1648  [1]
 LDCM0446  CL55 HEK-293T C370(1.05); C264(0.91)  LDD1649  [1]
 LDCM0447  CL56 HEK-293T C370(1.11); C264(1.55)  LDD1650  [1]
 LDCM0448  CL57 HEK-293T C370(1.05)  LDD1651  [1]
 LDCM0449  CL58 HEK-293T C370(1.04)  LDD1652  [1]
 LDCM0450  CL59 HEK-293T C370(0.88)  LDD1653  [1]
 LDCM0451  CL6 HEK-293T C370(0.82); C264(1.21)  LDD1654  [1]
 LDCM0452  CL60 HEK-293T C370(0.95)  LDD1655  [1]
 LDCM0453  CL61 HEK-293T C264(1.20)  LDD1656  [1]
 LDCM0454  CL62 HEK-293T C264(1.06)  LDD1657  [1]
 LDCM0455  CL63 HEK-293T C370(1.04); C264(1.09)  LDD1658  [1]
 LDCM0458  CL66 HEK-293T C370(0.95); C264(0.77)  LDD1661  [1]
 LDCM0459  CL67 HEK-293T C370(1.04); C264(0.80)  LDD1662  [1]
 LDCM0460  CL68 HEK-293T C370(1.22); C264(0.94)  LDD1663  [1]
 LDCM0461  CL69 HEK-293T C370(1.12)  LDD1664  [1]
 LDCM0462  CL7 HEK-293T C370(1.05); C264(1.04)  LDD1665  [1]
 LDCM0463  CL70 HEK-293T C370(1.05)  LDD1666  [1]
 LDCM0464  CL71 HEK-293T C370(0.93)  LDD1667  [1]
 LDCM0465  CL72 HEK-293T C370(0.96)  LDD1668  [1]
 LDCM0466  CL73 HEK-293T C264(0.98)  LDD1669  [1]
 LDCM0467  CL74 HEK-293T C264(1.04)  LDD1670  [1]
 LDCM0471  CL78 HEK-293T C370(1.04); C264(1.16)  LDD1674  [1]
 LDCM0472  CL79 HEK-293T C370(1.04); C264(1.02)  LDD1675  [1]
 LDCM0473  CL8 HEK-293T C370(0.93); C264(0.93)  LDD1676  [1]
 LDCM0474  CL80 HEK-293T C370(1.11); C264(1.18)  LDD1677  [1]
 LDCM0475  CL81 HEK-293T C370(1.14)  LDD1678  [1]
 LDCM0476  CL82 HEK-293T C370(0.98)  LDD1679  [1]
 LDCM0477  CL83 HEK-293T C370(0.90)  LDD1680  [1]
 LDCM0478  CL84 HEK-293T C370(0.92)  LDD1681  [1]
 LDCM0479  CL85 HEK-293T C264(0.89)  LDD1682  [1]
 LDCM0480  CL86 HEK-293T C264(0.94)  LDD1683  [1]
 LDCM0481  CL87 HEK-293T C370(0.86); C264(1.01)  LDD1684  [1]
 LDCM0484  CL9 HEK-293T C370(1.00)  LDD1687  [1]
 LDCM0485  CL90 HEK-293T C370(0.84); C264(1.06)  LDD1688  [1]
 LDCM0486  CL91 HEK-293T C370(0.95); C264(0.79)  LDD1689  [1]
 LDCM0487  CL92 HEK-293T C370(1.10); C264(1.12)  LDD1690  [1]
 LDCM0488  CL93 HEK-293T C370(1.13)  LDD1691  [1]
 LDCM0489  CL94 HEK-293T C370(0.93)  LDD1692  [1]
 LDCM0490  CL95 HEK-293T C370(0.87)  LDD1693  [1]
 LDCM0491  CL96 HEK-293T C370(1.25)  LDD1694  [1]
 LDCM0492  CL97 HEK-293T C264(1.04)  LDD1695  [1]
 LDCM0493  CL98 HEK-293T C264(1.12)  LDD1696  [1]
 LDCM0494  CL99 HEK-293T C370(0.93); C264(0.91)  LDD1697  [1]
 LDCM0495  E2913 HEK-293T C370(0.96); C264(1.08)  LDD1698  [1]
 LDCM0468  Fragment33 HEK-293T C370(1.04); C264(0.95)  LDD1671  [1]
 LDCM0427  Fragment51 HEK-293T C264(1.13)  LDD1631  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Fructose-bisphosphate aldolase B (ALDOB) Class I fructose-bisphosphate aldolase family P05062
Protein disulfide-isomerase (P4HB) Protein disulfide isomerase family P07237
Transporter and channel
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Pericentriolar material 1 protein (PCM1) PCM1 family Q15154
Bardet-Biedl syndrome 1 protein (BBS1) . Q8NFJ9
Protein PTHB1 (BBS9) . Q3SYG4
Transcription factor
Click To Hide/Show 5 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Zinc finger protein ZIC 1 (ZIC1) GLI C2H2-type zinc-finger protein family Q15915
Retinoic acid receptor alpha (RARA) Nuclear hormone receptor family P10276
Retinoic acid receptor gamma (RARG) Nuclear hormone receptor family P13631
Endothelial PAS domain-containing protein 1 (EPAS1) . Q99814
Myogenin (MYOG) . P15173
Other
Click To Hide/Show 6 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
BBSome-interacting protein 1 (BBIP1) BBIP10 family A8MTZ0
Dynactin subunit 1 (DCTN1) Dynactin 150 kDa subunit family Q14203
Protein spire homolog 1 (SPIRE1) Spire family Q08AE8
Cation channel sperm-associated targeting subunit tau (C2CD6) . Q53TS8
Coiled-coil domain-containing protein 28B (CCDC28B) . Q9BUN5
IQ calmodulin-binding motif-containing protein 1 (IQCB1) . Q15051

References

1 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
2 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 Chemoproteomic profiling of targets of lipid-derived electrophiles by bioorthogonal aminooxy probe. Redox Biol. 2017 Aug;12:712-718. doi: 10.1016/j.redox.2017.04.001. Epub 2017 Apr 5.
5 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells. Science. 2024 Apr 26;384(6694):eadk5864. doi: 10.1126/science.adk5864. Epub 2024 Apr 26.
Mass spectrometry data entry: PXD041587