Details of the Target

General Information of Target

Target ID LDTP09591
Target Name DDB1- and CUL4-associated factor 4 (DCAF4)
Gene Name DCAF4
Gene ID 26094
Synonyms
WDR21; WDR21A; DDB1- and CUL4-associated factor 4; WD repeat-containing protein 21A
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MNKSRWQSRRRHGRRSHQQNPWFRLRDSEDRSDSRAAQPAHDSGHGDDESPSTSSGTAGT
SSVPELPGFYFDPEKKRYFRLLPGHNNCNPLTKESIRQKEMESKRLRLLQEEDRRKKIAR
MGFNASSMLRKSQLGFLNVTNYCHLAHELRLSCMERKKVQIRSMDPSALASDRFNLILAD
TNSDRLFTVNDVKVGGSKYGIINLQSLKTPTLKVFMHENLYFTNRKVNSVCWASLNHLDS
HILLCLMGLAETPGCATLLPASLFVNSHPGIDRPGMLCSFRIPGAWSCAWSLNIQANNCF
STGLSRRVLLTNVVTGHRQSFGTNSDVLAQQFALMAPLLFNGCRSGEIFAIDLRCGNQGK
GWKATRLFHDSAVTSVRILQDEQYLMASDMAGKIKLWDLRTTKCVRQYEGHVNEYAYLPL
HVHEEEGILVAVGQDCYTRIWSLHDARLLRTIPSPYPASKADIPSVAFSSRLGGSRGAPG
LLMAVGQDLYCYSYS
Target Bioclass
Other
Function May function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex.
Uniprot ID
Q8WV16
Ensemble ID
ENST00000358377.7
HGNC ID
HGNC:20229

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
HEC1 SNV: p.S54L .
MV411 SNV: p.I394L .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
BTD
 Probe Info  		C355(0.63)  LDD2121  [1]
DBIA
 Probe Info  		C88(1.04)  LDD0531  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C88(1.04)  LDD0531  [2]
 LDCM0215  AC10 HEK-293T C88(0.99)  LDD1508  [4]
 LDCM0226  AC11 HEK-293T C88(1.09)  LDD1509  [4]
 LDCM0237  AC12 HEK-293T C88(0.90)  LDD1510  [4]
 LDCM0259  AC14 HEK-293T C88(1.05)  LDD1512  [4]
 LDCM0270  AC15 HEK-293T C88(0.98)  LDD1513  [4]
 LDCM0276  AC17 HCT 116 C88(0.91)  LDD0593  [2]
 LDCM0277  AC18 HCT 116 C88(1.04)  LDD0594  [2]
 LDCM0278  AC19 HCT 116 C88(0.99)  LDD0595  [2]
 LDCM0279  AC2 HCT 116 C88(0.95)  LDD0596  [2]
 LDCM0280  AC20 HCT 116 C88(1.09)  LDD0597  [2]
 LDCM0281  AC21 HCT 116 C88(1.02)  LDD0598  [2]
 LDCM0282  AC22 HCT 116 C88(1.30)  LDD0599  [2]
 LDCM0283  AC23 HCT 116 C88(1.00)  LDD0600  [2]
 LDCM0284  AC24 HCT 116 C88(0.80)  LDD0601  [2]
 LDCM0285  AC25 HCT 116 C88(1.07)  LDD0602  [2]
 LDCM0286  AC26 HCT 116 C88(0.93)  LDD0603  [2]
 LDCM0287  AC27 HCT 116 C88(0.88)  LDD0604  [2]
 LDCM0288  AC28 HCT 116 C88(0.88)  LDD0605  [2]
 LDCM0289  AC29 HCT 116 C88(0.71)  LDD0606  [2]
 LDCM0290  AC3 HCT 116 C88(0.81)  LDD0607  [2]
 LDCM0291  AC30 HCT 116 C88(0.69)  LDD0608  [2]
 LDCM0292  AC31 HCT 116 C88(0.77)  LDD0609  [2]
 LDCM0293  AC32 HCT 116 C88(0.66)  LDD0610  [2]
 LDCM0294  AC33 HCT 116 C88(0.82)  LDD0611  [2]
 LDCM0295  AC34 HCT 116 C88(0.75)  LDD0612  [2]
 LDCM0296  AC35 HEK-293T C88(0.91)  LDD1535  [4]
 LDCM0297  AC36 HEK-293T C88(1.07)  LDD1536  [4]
 LDCM0298  AC37 HEK-293T C88(0.91)  LDD1537  [4]
 LDCM0299  AC38 HEK-293T C88(1.28)  LDD1538  [4]
 LDCM0300  AC39 HEK-293T C88(1.02)  LDD1539  [4]
 LDCM0301  AC4 HCT 116 C88(0.79)  LDD0618  [2]
 LDCM0303  AC41 HEK-293T C88(0.92)  LDD1542  [4]
 LDCM0304  AC42 HEK-293T C88(0.93)  LDD1543  [4]
 LDCM0305  AC43 HEK-293T C88(0.96)  LDD1544  [4]
 LDCM0306  AC44 HEK-293T C88(0.93)  LDD1545  [4]
 LDCM0307  AC45 HEK-293T C88(1.06)  LDD1546  [4]
 LDCM0308  AC46 HEK-293T C88(1.11)  LDD1547  [4]
 LDCM0309  AC47 HEK-293T C88(0.99)  LDD1548  [4]
 LDCM0311  AC49 HEK-293T C88(0.97)  LDD1550  [4]
 LDCM0312  AC5 HCT 116 C88(0.88)  LDD0629  [2]
 LDCM0313  AC50 HEK-293T C88(1.01)  LDD1552  [4]
 LDCM0314  AC51 HEK-293T C88(0.91)  LDD1553  [4]
 LDCM0315  AC52 HEK-293T C88(1.07)  LDD1554  [4]
 LDCM0316  AC53 HEK-293T C88(1.04)  LDD1555  [4]
 LDCM0317  AC54 HEK-293T C88(1.11)  LDD1556  [4]
 LDCM0318  AC55 HEK-293T C88(0.99)  LDD1557  [4]
 LDCM0320  AC57 HEK-293T C88(1.06)  LDD1559  [4]
 LDCM0321  AC58 HEK-293T C88(0.96)  LDD1560  [4]
 LDCM0322  AC59 HEK-293T C88(1.12)  LDD1561  [4]
 LDCM0323  AC6 HEK-293T C88(1.16)  LDD1562  [4]
 LDCM0324  AC60 HEK-293T C88(1.01)  LDD1563  [4]
 LDCM0325  AC61 HEK-293T C88(1.09)  LDD1564  [4]
 LDCM0326  AC62 HEK-293T C88(1.06)  LDD1565  [4]
 LDCM0327  AC63 HEK-293T C88(1.01)  LDD1566  [4]
 LDCM0334  AC7 HEK-293T C88(0.96)  LDD1568  [4]
 LDCM0248  AKOS034007472 HEK-293T C88(0.98)  LDD1511  [4]
 LDCM0356  AKOS034007680 HEK-293T C88(1.10)  LDD1570  [4]
 LDCM0368  CL10 HEK-293T C88(1.24)  LDD1572  [4]
 LDCM0369  CL100 HCT 116 C88(1.09)  LDD0686  [2]
 LDCM0371  CL102 HEK-293T C88(0.91)  LDD1575  [4]
 LDCM0372  CL103 HEK-293T C88(1.06)  LDD1576  [4]
 LDCM0374  CL105 HCT 116 C88(1.17)  LDD0691  [2]
 LDCM0375  CL106 HCT 116 C88(1.15)  LDD0692  [2]
 LDCM0376  CL107 HCT 116 C88(1.17)  LDD0693  [2]
 LDCM0377  CL108 HCT 116 C88(0.84)  LDD0694  [2]
 LDCM0378  CL109 HCT 116 C88(1.17)  LDD0695  [2]
 LDCM0379  CL11 HEK-293T C88(1.04)  LDD1583  [4]
 LDCM0380  CL110 HCT 116 C88(1.06)  LDD0697  [2]
 LDCM0381  CL111 HCT 116 C88(1.11)  LDD0698  [2]
 LDCM0382  CL112 HCT 116 C88(0.89)  LDD0699  [2]
 LDCM0383  CL113 HCT 116 C88(0.63)  LDD0700  [2]
 LDCM0384  CL114 HCT 116 C88(0.54)  LDD0701  [2]
 LDCM0385  CL115 HCT 116 C88(0.64)  LDD0702  [2]
 LDCM0386  CL116 HCT 116 C88(0.77)  LDD0703  [2]
 LDCM0388  CL118 HEK-293T C88(1.01)  LDD1592  [4]
 LDCM0389  CL119 HEK-293T C88(1.01)  LDD1593  [4]
 LDCM0393  CL122 HEK-293T C88(0.96)  LDD1597  [4]
 LDCM0394  CL123 HEK-293T C88(0.87)  LDD1598  [4]
 LDCM0397  CL126 HEK-293T C88(0.85)  LDD1601  [4]
 LDCM0398  CL127 HEK-293T C88(1.06)  LDD1602  [4]
 LDCM0401  CL14 HEK-293T C88(1.10)  LDD1605  [4]
 LDCM0402  CL15 HEK-293T C88(1.02)  LDD1606  [4]
 LDCM0403  CL16 HCT 116 C88(1.42)  LDD0720  [2]
 LDCM0404  CL17 HCT 116 C88(0.84)  LDD0721  [2]
 LDCM0405  CL18 HCT 116 C88(0.95)  LDD0722  [2]
 LDCM0406  CL19 HCT 116 C88(1.15)  LDD0723  [2]
 LDCM0407  CL2 HEK-293T C88(1.10)  LDD1611  [4]
 LDCM0408  CL20 HCT 116 C88(1.27)  LDD0725  [2]
 LDCM0409  CL21 HCT 116 C88(1.19)  LDD0726  [2]
 LDCM0410  CL22 HCT 116 C88(1.24)  LDD0727  [2]
 LDCM0411  CL23 HCT 116 C88(1.12)  LDD0728  [2]
 LDCM0412  CL24 HCT 116 C88(0.92)  LDD0729  [2]
 LDCM0413  CL25 HCT 116 C88(1.03)  LDD0730  [2]
 LDCM0414  CL26 HCT 116 C88(1.25)  LDD0731  [2]
 LDCM0415  CL27 HCT 116 C88(1.13)  LDD0732  [2]
 LDCM0416  CL28 HCT 116 C88(1.18)  LDD0733  [2]
 LDCM0417  CL29 HCT 116 C88(1.10)  LDD0734  [2]
 LDCM0418  CL3 HEK-293T C88(1.04)  LDD1622  [4]
 LDCM0419  CL30 HCT 116 C88(1.39)  LDD0736  [2]
 LDCM0420  CL31 HCT 116 C88(1.05)  LDD0737  [2]
 LDCM0421  CL32 HCT 116 C88(1.12)  LDD0738  [2]
 LDCM0422  CL33 HCT 116 C88(1.17)  LDD0739  [2]
 LDCM0423  CL34 HCT 116 C88(1.20)  LDD0740  [2]
 LDCM0424  CL35 HCT 116 C88(1.11)  LDD0741  [2]
 LDCM0425  CL36 HCT 116 C88(1.16)  LDD0742  [2]
 LDCM0426  CL37 HCT 116 C88(1.12)  LDD0743  [2]
 LDCM0428  CL39 HCT 116 C88(0.91)  LDD0745  [2]
 LDCM0430  CL40 HCT 116 C88(1.13)  LDD0747  [2]
 LDCM0431  CL41 HCT 116 C88(1.09)  LDD0748  [2]
 LDCM0432  CL42 HCT 116 C88(1.07)  LDD0749  [2]
 LDCM0433  CL43 HCT 116 C88(1.12)  LDD0750  [2]
 LDCM0434  CL44 HCT 116 C88(1.16)  LDD0751  [2]
 LDCM0435  CL45 HCT 116 C88(1.12)  LDD0752  [2]
 LDCM0436  CL46 HEK-293T C88(1.34)  LDD1640  [4]
 LDCM0437  CL47 HEK-293T C88(0.95)  LDD1641  [4]
 LDCM0440  CL5 HEK-293T C88(0.89)  LDD1644  [4]
 LDCM0441  CL50 HEK-293T C88(0.97)  LDD1645  [4]
 LDCM0444  CL53 HEK-293T C88(0.93)  LDD1647  [4]
 LDCM0445  CL54 HEK-293T C88(0.99)  LDD1648  [4]
 LDCM0446  CL55 HEK-293T C88(0.99)  LDD1649  [4]
 LDCM0447  CL56 HEK-293T C88(0.97)  LDD1650  [4]
 LDCM0448  CL57 HEK-293T C88(1.08)  LDD1651  [4]
 LDCM0449  CL58 HEK-293T C88(1.14)  LDD1652  [4]
 LDCM0450  CL59 HEK-293T C88(0.91)  LDD1653  [4]
 LDCM0451  CL6 HEK-293T C88(0.91)  LDD1654  [4]
 LDCM0454  CL62 HEK-293T C88(1.15)  LDD1657  [4]
 LDCM0455  CL63 HEK-293T C88(0.97)  LDD1658  [4]
 LDCM0457  CL65 HEK-293T C88(1.07)  LDD1660  [4]
 LDCM0458  CL66 HEK-293T C88(0.95)  LDD1661  [4]
 LDCM0459  CL67 HEK-293T C88(1.01)  LDD1662  [4]
 LDCM0460  CL68 HEK-293T C88(1.01)  LDD1663  [4]
 LDCM0461  CL69 HEK-293T C88(0.93)  LDD1664  [4]
 LDCM0462  CL7 HEK-293T C88(0.95)  LDD1665  [4]
 LDCM0463  CL70 HEK-293T C88(1.28)  LDD1666  [4]
 LDCM0464  CL71 HEK-293T C88(1.07)  LDD1667  [4]
 LDCM0467  CL74 HEK-293T C88(1.03)  LDD1670  [4]
 LDCM0470  CL77 HEK-293T C88(0.99)  LDD1673  [4]
 LDCM0471  CL78 HEK-293T C88(0.94)  LDD1674  [4]
 LDCM0472  CL79 HEK-293T C88(1.04)  LDD1675  [4]
 LDCM0473  CL8 HEK-293T C88(0.82)  LDD1676  [4]
 LDCM0474  CL80 HEK-293T C88(1.08)  LDD1677  [4]
 LDCM0475  CL81 HEK-293T C88(1.09)  LDD1678  [4]
 LDCM0476  CL82 HEK-293T C88(1.04)  LDD1679  [4]
 LDCM0477  CL83 HEK-293T C88(0.90)  LDD1680  [4]
 LDCM0480  CL86 HEK-293T C88(1.12)  LDD1683  [4]
 LDCM0481  CL87 HEK-293T C88(1.04)  LDD1684  [4]
 LDCM0483  CL89 HEK-293T C88(0.97)  LDD1686  [4]
 LDCM0484  CL9 HEK-293T C88(0.93)  LDD1687  [4]
 LDCM0485  CL90 HEK-293T C88(0.82)  LDD1688  [4]
 LDCM0486  CL91 HCT 116 C88(0.91)  LDD0803  [2]
 LDCM0487  CL92 HCT 116 C88(1.04)  LDD0804  [2]
 LDCM0488  CL93 HCT 116 C88(0.98)  LDD0805  [2]
 LDCM0489  CL94 HCT 116 C88(1.14)  LDD0806  [2]
 LDCM0490  CL95 HCT 116 C88(1.06)  LDD0807  [2]
 LDCM0491  CL96 HCT 116 C88(0.92)  LDD0808  [2]
 LDCM0492  CL97 HCT 116 C88(0.98)  LDD0809  [2]
 LDCM0493  CL98 HCT 116 C88(1.13)  LDD0810  [2]
 LDCM0494  CL99 HCT 116 C88(1.06)  LDD0811  [2]
 LDCM0495  E2913 HEK-293T C88(1.25)  LDD1698  [4]
 LDCM0468  Fragment33 HEK-293T C88(1.14)  LDD1671  [4]
 LDCM0427  Fragment51 HCT 116 C88(1.28)  LDD0744  [2]
 LDCM0022  KB02 EGI-1 C88(2.02)  LDD2323  [5]
 LDCM0023  KB03 EGI-1 C88(2.29)  LDD2740  [5]
 LDCM0024  KB05 PANC-1 C345(2.08)  LDD3392  [5]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C355(0.63)  LDD2121  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Ataxin-1 (ATXN1) ATXN1 family P54253
DNA damage-binding protein 1 (DDB1) DDB1 family Q16531
Suppressor of tumorigenicity 7 protein (ST7) ST7 family Q9NRC1

References

1 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
5 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840