Details of the Target

General Information of Target

Target ID LDTP09505
Target Name GPI ethanolamine phosphate transferase 3 (PIGO)
Gene Name PIGO
Gene ID 84720
Synonyms
GPI ethanolamine phosphate transferase 3; EC 2.-.-.-; Phosphatidylinositol-glycan biosynthesis class O protein; PIG-O
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MQKASVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNHSSCQEPPGPGSLPWGSQGKPGA
CWMASRFSRVVLVLIDALRFDFAQPQHSHVPREPPVSLPFLGKLSSLQRILEIQPHHARL
YRSQVDPPTTTMQRLKALTTGSLPTFIDAGSNFASHAIVEDNLIKQLTSAGRRVVFMGDD
TWKDLFPGAFSKAFFFPSFNVRDLDTVDNGILEHLYPTMDSGEWDVLIAHFLGVDHCGHK
HGPHHPEMAKKLSQMDQVIQGLVERLENDTLLVVAGDHGMTTNGDHGGDSELEVSAALFL
YSPTAVFPSTPPEEPEVIPQVSLVPTLALLLGLPIPFGNIGEVMAELFSGGEDSQPHSSA
LAQASALHLNAQQVSRFLHTYSAATQDLQAKELHQLQNLFSKASADYQWLLQSPKGAEAT
LPTVIAELQQFLRGARAMCIESWARFSLVRMAGGTALLAASCFICLLASQWAISPGFPFC
PLLLTPVAWGLVGAIAYAGLLGTIELKLDLVLLGAVAAVSSFLPFLWKAWAGWGSKRPLA
TLFPIPGPVLLLLLFRLAVFFSDSFVVAEARATPFLLGSFILLLVVQLHWEGQLLPPKLL
TMPRLGTSATTNPPRHNGAYALRLGIGLLLCTRLAGLFHRCPEETPVCHSSPWLSPLASM
VGGRAKNLWYGACVAALVALLAAVRLWLRRYGNLKSPEPPMLFVRWGLPLMALGTAAYWA
LASGADEAPPRLRVLVSGASMVLPRAVAGLAASGLALLLWKPVTVLVKAGAGAPRTRTVL
TPFSGPPTSQADLDYVVPQIYRHMQEEFRGRLERTKSQGPLTVAAYQLGSVYSAAMVTAL
TLLAFPLLLLHAERISLVFLLLFLQSFLLLHLLAAGIPVTTPGPFTVPWQAVSAWALMAT
QTFYSTGHQPVFPAIHWHAAFVGFPEGHGSCTWLPALLVGANTFASHLLFAVGCPLLLLW
PFLCESQGLRKRQQPPGNEADARVRPEEEEEPLMEMRLRDAPQHFYAALLQLGLKYLFIL
GIQILACALAASILRRHLMVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDGAVSSWF
RQLFLAQQR
Target Bioclass
Enzyme
Family
PIGG/PIGN/PIGO family, PIGO subfamily
Subcellular location
Endoplasmic reticulum membrane
Function
Ethanolamine phosphate transferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers ethanolamine phosphate to the GPI third mannose which links the GPI-anchor to the C-terminus of the proteins by an amide bond.
Uniprot ID
Q8TEQ8
Ensemble ID
ENST00000298004.9
HGNC ID
HGNC:23215

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
DU145 SNV: p.Q429Ter .
HCT116 Insertion: p.T788HfsTer5
SNV: p.A658V		 .
IM95 Deletion: p.H639IfsTer16; p.T788LfsTer25 .
IPC298 Substitution: p.G785N .
JHH7 SNV: p.S784Ter .
JURKAT SNV: p.Q395R .
MDAMB231 SNV: p.V226L .
NB4 SNV: p.P45L .
NCIH1155 SNV: p.G907A .
NCIH1792 SNV: p.A170E .
NCIH1793 SNV: p.Y718F .
NCIH716 Deletion: p.T906PfsTer58 .
SW480 SNV: p.P115L .
SW756 SNV: p.P356T .
UACC257 SNV: p.S650F .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C439(2.33)  LDD3383  [1]
Johansson_61
 Probe Info  		_(20.00)  LDD1485  [2]
PAL-AfBPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
A-DA
 Probe Info  		2.28  LDD0145  [3]
OEA-DA
 Probe Info  		9.64  LDD0046  [4]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0615  Fragment63-R Jurkat _(20.00)  LDD1487  [2]
 LDCM0617  Fragment63-S Jurkat _(20.00)  LDD1490  [2]
 LDCM0569  Fragment7 Jurkat _(20.00)  LDD1485  [2]
 LDCM0022  KB02 A2780 C439(1.48)  LDD2254  [1]
 LDCM0023  KB03 A2780 C439(2.12)  LDD2671  [1]
 LDCM0024  KB05 OVCAR-5 C439(2.33)  LDD3383  [1]
 LDCM0084  Ro 48-8071 A-549 2.28  LDD0145  [3]

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Proteome-wide covalent ligand discovery in native biological systems. Nature. 2016 Jun 23;534(7608):570-4. doi: 10.1038/nature18002. Epub 2016 Jun 15.
3 A Global Map of Lipid-Binding Proteins and Their Ligandability in Cells. Cell. 2015 Jun 18;161(7):1668-80. doi: 10.1016/j.cell.2015.05.045.
4 Mapping Protein Targets of Bioactive Small Molecules Using Lipid-Based Chemical Proteomics. ACS Chem Biol. 2017 Oct 20;12(10):2671-2681. doi: 10.1021/acschembio.7b00581. Epub 2017 Sep 20.
Mass spectrometry data entry: PXD007570