Details of the Target

General Information of Target

Target ID LDTP09434
Target Name Helicase POLQ-like (HELQ)
Gene Name HELQ
Gene ID 113510
Synonyms
HEL308; Helicase POLQ-like; EC 3.6.4.12; Mus308-like helicase; POLQ-like helicase
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDECGSRIRRRVSLPKRNRPSLGCIFGAPTAAELVPGDEGKEEEEMVAENRRRKTAGVLP
VEVQPLLLSDSPECLVLGGGDTNPDLLRHMPTDRGVGDQPNDSEVDMFGDYDSFTENSFI
AQVDDLEQKYMQLPEHKKHATDFATENLCSESIKNKLSITTIGNLTELQTDKHTENQSGY
EGVTIEPGADLLYDVPSSQAIYFENLQNSSNDLGDHSMKERDWKSSSHNTVNEELPHNCI
EQPQQNDESSSKVRTSSDMNRRKSIKDHLKNAMTGNAKAQTPIFSRSKQLKDTLLSEEIN
VAKKTVESSSNDLGPFYSLPSKVRDLYAQFKGIEKLYEWQHTCLTLNSVQERKNLIYSLP
TSGGKTLVAEILMLQELLCCRKDVLMILPYVAIVQEKISGLSSFGIELGFFVEEYAGSKG
RFPPTKRREKKSLYIATIEKGHSLVNSLIETGRIDSLGLVVVDELHMIGEGSRGATLEMT
LAKILYTSKTTQIIGMSATLNNVEDLQKFLQAEYYTSQFRPVELKEYLKINDTIYEVDSK
AENGMTFSRLLNYKYSDTLKKMDPDHLVALVTEVIPNYSCLVFCPSKKNCENVAEMICKF
LSKEYLKHKEKEKCEVIKNLKNIGNGNLCPVLKRTIPFGVAYHHSGLTSDERKLLEEAYS
TGVLCLFTCTSTLAAGVNLPARRVILRAPYVAKEFLKRNQYKQMIGRAGRAGIDTIGESI
LILQEKDKQQVLELITKPLENCYSHLVQEFTKGIQTLFLSLIGLKIATNLDDIYHFMNGT
FFGVQQKVLLKEKSLWEITVESLRYLTEKGLLQKDTIYKSEEEVQYNFHITKLGRASFKG
TIDLAYCDILYRDLKKGLEGLVLESLLHLIYLTTPYDLVSQCNPDWMIYFRQFSQLSPAE
QNVAAILGVSESFIGKKASGQAIGKKVDKNVVNRLYLSFVLYTLLKETNIWTVSEKFNMP
RGYIQNLLTGTASFSSCVLHFCEELEEFWVYRALLVELTKKLTYCVKAELIPLMEVTGVL
EGRAKQLYSAGYKSLMHLANANPEVLVRTIDHLSRRQAKQIVSSAKMLLHEKAEALQEEV
EELLRLPSDFPGAVASSTDKA
Target Bioclass
Enzyme
Family
Helicase family, SKI2 subfamily
Subcellular location
Nucleus
Function
Single-stranded 3'-5' DNA helicase that plays a key role in homology-driven double-strand break (DSB) repair. Involved in different DSB repair mechanisms that are guided by annealing of extensive stretches of complementary bases at break ends, such as microhomology-mediated end-joining (MMEJ), single-strand annealing (SSA) or synthesis-dependent strand annealing (SDSA). Possesses both DNA unwinding and annealing activities. Forms a complex with RAD51, stimulating HELQ DNA helicase activity and ability to unwing DNA. Efficiently unwinds substrates containing 3' overhangs or a D-loop. In contrast, interaction with the replication protein A (RPA/RP-A) complex inhibits DNA unwinding by HELQ but strongly stimulates DNA strand annealing. Triggers displacement of RPA from single-stranded DNA to facilitate annealing of complementary sequences.
Uniprot ID
Q8TDG4
Ensemble ID
ENST00000295488.8
HGNC ID
HGNC:18536

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A172 SNV: p.V349E .
CAL148 SNV: p.R1023Q .
FTC133 SNV: p.Q724Ter .
HSC3 SNV: p.I283M .
HT SNV: p.N446S .
KATOIII SNV: p.A1101E .
KMRC1 Deletion: p.R687SfsTer2 .
MFE319 SNV: p.G857C .
ONS76 SNV: p.V568E .
SNGM SNV: p.A272D .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 3 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C629(0.30)  LDD2196  [1]
DBIA
 Probe Info  		C629(0.98)  LDD1507  [2]
IPM
 Probe Info  		N.A.  LDD2156  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C629(0.98)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C629(1.20)  LDD1508  [2]
 LDCM0226  AC11 HEK-293T C629(1.22)  LDD1509  [2]
 LDCM0237  AC12 HEK-293T C629(1.16)  LDD1510  [2]
 LDCM0259  AC14 HEK-293T C629(0.99)  LDD1512  [2]
 LDCM0270  AC15 HEK-293T C629(1.07)  LDD1513  [2]
 LDCM0276  AC17 HEK-293T C629(1.02)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C629(1.04)  LDD1516  [2]
 LDCM0278  AC19 HEK-293T C629(1.03)  LDD1517  [2]
 LDCM0279  AC2 HEK-293T C629(1.14)  LDD1518  [2]
 LDCM0280  AC20 HEK-293T C629(1.20)  LDD1519  [2]
 LDCM0282  AC22 HEK-293T C629(1.00)  LDD1521  [2]
 LDCM0283  AC23 HEK-293T C629(1.03)  LDD1522  [2]
 LDCM0284  AC24 HEK-293T C629(0.99)  LDD1523  [2]
 LDCM0285  AC25 HEK-293T C629(0.97)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C629(1.10)  LDD1525  [2]
 LDCM0287  AC27 HEK-293T C629(1.04)  LDD1526  [2]
 LDCM0288  AC28 HEK-293T C629(1.23)  LDD1527  [2]
 LDCM0290  AC3 HEK-293T C629(1.08)  LDD1529  [2]
 LDCM0291  AC30 HEK-293T C629(1.00)  LDD1530  [2]
 LDCM0292  AC31 HEK-293T C629(1.00)  LDD1531  [2]
 LDCM0293  AC32 HEK-293T C629(1.03)  LDD1532  [2]
 LDCM0294  AC33 HEK-293T C629(1.04)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C629(1.19)  LDD1534  [2]
 LDCM0296  AC35 HEK-293T C629(1.02)  LDD1535  [2]
 LDCM0297  AC36 HEK-293T C629(1.30)  LDD1536  [2]
 LDCM0299  AC38 HEK-293T C629(1.07)  LDD1538  [2]
 LDCM0300  AC39 HEK-293T C629(0.99)  LDD1539  [2]
 LDCM0301  AC4 HEK-293T C629(1.21)  LDD1540  [2]
 LDCM0302  AC40 HEK-293T C629(1.02)  LDD1541  [2]
 LDCM0303  AC41 HEK-293T C629(1.07)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C629(1.22)  LDD1543  [2]
 LDCM0305  AC43 HEK-293T C629(1.07)  LDD1544  [2]
 LDCM0306  AC44 HEK-293T C629(1.13)  LDD1545  [2]
 LDCM0308  AC46 HEK-293T C629(1.05)  LDD1547  [2]
 LDCM0309  AC47 HEK-293T C629(1.07)  LDD1548  [2]
 LDCM0310  AC48 HEK-293T C629(1.00)  LDD1549  [2]
 LDCM0311  AC49 HEK-293T C629(0.97)  LDD1550  [2]
 LDCM0313  AC50 HEK-293T C629(1.13)  LDD1552  [2]
 LDCM0314  AC51 HEK-293T C629(1.00)  LDD1553  [2]
 LDCM0315  AC52 HEK-293T C629(1.08)  LDD1554  [2]
 LDCM0317  AC54 HEK-293T C629(0.98)  LDD1556  [2]
 LDCM0318  AC55 HEK-293T C629(1.14)  LDD1557  [2]
 LDCM0319  AC56 HEK-293T C629(1.01)  LDD1558  [2]
 LDCM0320  AC57 HEK-293T C629(1.11)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C629(1.20)  LDD1560  [2]
 LDCM0322  AC59 HEK-293T C629(0.89)  LDD1561  [2]
 LDCM0323  AC6 HEK-293T C629(1.06)  LDD1562  [2]
 LDCM0324  AC60 HEK-293T C629(1.25)  LDD1563  [2]
 LDCM0326  AC62 HEK-293T C629(0.98)  LDD1565  [2]
 LDCM0327  AC63 HEK-293T C629(1.09)  LDD1566  [2]
 LDCM0328  AC64 HEK-293T C629(1.00)  LDD1567  [2]
 LDCM0334  AC7 HEK-293T C629(1.09)  LDD1568  [2]
 LDCM0345  AC8 HEK-293T C629(1.03)  LDD1569  [2]
 LDCM0356  AKOS034007680 HEK-293T C629(1.05)  LDD1570  [2]
 LDCM0275  AKOS034007705 HEK-293T C629(1.01)  LDD1514  [2]
 LDCM0368  CL10 HEK-293T C629(1.11)  LDD1572  [2]
 LDCM0369  CL100 HEK-293T C629(0.87)  LDD1573  [2]
 LDCM0371  CL102 HEK-293T C629(1.23)  LDD1575  [2]
 LDCM0372  CL103 HEK-293T C629(1.07)  LDD1576  [2]
 LDCM0373  CL104 HEK-293T C629(1.06)  LDD1577  [2]
 LDCM0375  CL106 HEK-293T C629(1.16)  LDD1579  [2]
 LDCM0376  CL107 HEK-293T C629(1.08)  LDD1580  [2]
 LDCM0377  CL108 HEK-293T C629(1.09)  LDD1581  [2]
 LDCM0379  CL11 HEK-293T C629(0.93)  LDD1583  [2]
 LDCM0380  CL110 HEK-293T C629(0.99)  LDD1584  [2]
 LDCM0381  CL111 HEK-293T C629(1.05)  LDD1585  [2]
 LDCM0382  CL112 HEK-293T C629(1.05)  LDD1586  [2]
 LDCM0384  CL114 HEK-293T C629(1.14)  LDD1588  [2]
 LDCM0385  CL115 HEK-293T C629(1.05)  LDD1589  [2]
 LDCM0386  CL116 HEK-293T C629(1.14)  LDD1590  [2]
 LDCM0388  CL118 HEK-293T C629(1.06)  LDD1592  [2]
 LDCM0389  CL119 HEK-293T C629(1.10)  LDD1593  [2]
 LDCM0390  CL12 HEK-293T C629(1.06)  LDD1594  [2]
 LDCM0391  CL120 HEK-293T C629(1.10)  LDD1595  [2]
 LDCM0393  CL122 HEK-293T C629(1.12)  LDD1597  [2]
 LDCM0394  CL123 HEK-293T C629(1.10)  LDD1598  [2]
 LDCM0395  CL124 HEK-293T C629(0.91)  LDD1599  [2]
 LDCM0397  CL126 HEK-293T C629(1.23)  LDD1601  [2]
 LDCM0398  CL127 HEK-293T C629(1.09)  LDD1602  [2]
 LDCM0399  CL128 HEK-293T C629(1.08)  LDD1603  [2]
 LDCM0401  CL14 HEK-293T C629(1.27)  LDD1605  [2]
 LDCM0402  CL15 HEK-293T C629(1.13)  LDD1606  [2]
 LDCM0403  CL16 HEK-293T C629(1.17)  LDD1607  [2]
 LDCM0404  CL17 HEK-293T C629(1.07)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C629(1.11)  LDD1609  [2]
 LDCM0406  CL19 HEK-293T C629(1.16)  LDD1610  [2]
 LDCM0407  CL2 HEK-293T C629(1.18)  LDD1611  [2]
 LDCM0408  CL20 HEK-293T C629(1.17)  LDD1612  [2]
 LDCM0410  CL22 HEK-293T C629(1.06)  LDD1614  [2]
 LDCM0411  CL23 HEK-293T C629(0.92)  LDD1615  [2]
 LDCM0412  CL24 HEK-293T C629(1.17)  LDD1616  [2]
 LDCM0414  CL26 HEK-293T C629(1.11)  LDD1618  [2]
 LDCM0415  CL27 HEK-293T C629(1.00)  LDD1619  [2]
 LDCM0416  CL28 HEK-293T C629(0.96)  LDD1620  [2]
 LDCM0417  CL29 HEK-293T C629(1.06)  LDD1621  [2]
 LDCM0418  CL3 HEK-293T C629(1.26)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C629(1.06)  LDD1623  [2]
 LDCM0420  CL31 HEK-293T C629(0.98)  LDD1624  [2]
 LDCM0421  CL32 HEK-293T C629(0.99)  LDD1625  [2]
 LDCM0423  CL34 HEK-293T C629(1.00)  LDD1627  [2]
 LDCM0424  CL35 HEK-293T C629(0.89)  LDD1628  [2]
 LDCM0425  CL36 HEK-293T C629(0.97)  LDD1629  [2]
 LDCM0428  CL39 HEK-293T C629(1.04)  LDD1632  [2]
 LDCM0429  CL4 HEK-293T C629(1.17)  LDD1633  [2]
 LDCM0430  CL40 HEK-293T C629(0.97)  LDD1634  [2]
 LDCM0431  CL41 HEK-293T C629(1.23)  LDD1635  [2]
 LDCM0432  CL42 HEK-293T C629(0.99)  LDD1636  [2]
 LDCM0433  CL43 HEK-293T C629(1.07)  LDD1637  [2]
 LDCM0434  CL44 HEK-293T C629(1.08)  LDD1638  [2]
 LDCM0436  CL46 HEK-293T C629(1.00)  LDD1640  [2]
 LDCM0437  CL47 HEK-293T C629(0.89)  LDD1641  [2]
 LDCM0438  CL48 HEK-293T C629(1.17)  LDD1642  [2]
 LDCM0440  CL5 HEK-293T C629(1.00)  LDD1644  [2]
 LDCM0441  CL50 HEK-293T C629(1.21)  LDD1645  [2]
 LDCM0443  CL52 HEK-293T C629(0.97)  LDD1646  [2]
 LDCM0444  CL53 HEK-293T C629(1.02)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C629(1.09)  LDD1648  [2]
 LDCM0446  CL55 HEK-293T C629(0.96)  LDD1649  [2]
 LDCM0447  CL56 HEK-293T C629(1.10)  LDD1650  [2]
 LDCM0449  CL58 HEK-293T C629(1.06)  LDD1652  [2]
 LDCM0450  CL59 HEK-293T C629(0.96)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C629(1.11)  LDD1654  [2]
 LDCM0452  CL60 HEK-293T C629(1.11)  LDD1655  [2]
 LDCM0454  CL62 HEK-293T C629(1.22)  LDD1657  [2]
 LDCM0455  CL63 HEK-293T C629(1.09)  LDD1658  [2]
 LDCM0456  CL64 HEK-293T C629(1.23)  LDD1659  [2]
 LDCM0457  CL65 HEK-293T C629(1.04)  LDD1660  [2]
 LDCM0458  CL66 HEK-293T C629(1.02)  LDD1661  [2]
 LDCM0459  CL67 HEK-293T C629(0.99)  LDD1662  [2]
 LDCM0460  CL68 HEK-293T C629(0.98)  LDD1663  [2]
 LDCM0462  CL7 HEK-293T C629(1.13)  LDD1665  [2]
 LDCM0463  CL70 HEK-293T C629(1.08)  LDD1666  [2]
 LDCM0464  CL71 HEK-293T C629(0.86)  LDD1667  [2]
 LDCM0465  CL72 HEK-293T C629(1.15)  LDD1668  [2]
 LDCM0467  CL74 HEK-293T C629(1.22)  LDD1670  [2]
 LDCM0469  CL76 HEK-293T C629(1.15)  LDD1672  [2]
 LDCM0470  CL77 HEK-293T C629(1.04)  LDD1673  [2]
 LDCM0471  CL78 HEK-293T C629(1.09)  LDD1674  [2]
 LDCM0472  CL79 HEK-293T C629(0.97)  LDD1675  [2]
 LDCM0473  CL8 HEK-293T C629(1.04)  LDD1676  [2]
 LDCM0474  CL80 HEK-293T C629(1.02)  LDD1677  [2]
 LDCM0476  CL82 HEK-293T C629(0.98)  LDD1679  [2]
 LDCM0477  CL83 HEK-293T C629(1.02)  LDD1680  [2]
 LDCM0478  CL84 HEK-293T C629(1.09)  LDD1681  [2]
 LDCM0480  CL86 HEK-293T C629(1.13)  LDD1683  [2]
 LDCM0481  CL87 HEK-293T C629(0.96)  LDD1684  [2]
 LDCM0482  CL88 HEK-293T C629(0.99)  LDD1685  [2]
 LDCM0483  CL89 HEK-293T C629(1.02)  LDD1686  [2]
 LDCM0485  CL90 HEK-293T C629(1.36)  LDD1688  [2]
 LDCM0486  CL91 HEK-293T C629(1.01)  LDD1689  [2]
 LDCM0487  CL92 HEK-293T C629(1.21)  LDD1690  [2]
 LDCM0489  CL94 HEK-293T C629(1.00)  LDD1692  [2]
 LDCM0490  CL95 HEK-293T C629(0.86)  LDD1693  [2]
 LDCM0491  CL96 HEK-293T C629(1.08)  LDD1694  [2]
 LDCM0493  CL98 HEK-293T C629(1.26)  LDD1696  [2]
 LDCM0494  CL99 HEK-293T C629(0.97)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C629(1.17)  LDD1698  [2]
 LDCM0582  Fragment23 Ramos C629(0.30)  LDD2196  [1]
 LDCM0578  Fragment27 Ramos C629(0.72)  LDD2197  [1]
 LDCM0586  Fragment28 Ramos C629(1.80)  LDD2198  [1]
 LDCM0468  Fragment33 HEK-293T C629(0.98)  LDD1671  [2]
 LDCM0596  Fragment38 Ramos C629(0.79)  LDD2203  [1]
 LDCM0427  Fragment51 HEK-293T C629(1.02)  LDD1631  [2]
 LDCM0614  Fragment56 Ramos C629(0.98)  LDD2205  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Other
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA repair protein RAD51 homolog 3 (RAD51C) RecA family O43502

References

1 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019