Details of the Target

General Information of Target

Target ID LDTP09004
Target Name Peroxisomal succinyl-coenzyme A thioesterase (ACOT4)
Gene Name ACOT4
Gene ID 122970
Synonyms
PTE2B; PTEIB; Peroxisomal succinyl-coenzyme A thioesterase; EC 3.1.2.3; Acyl-coenzyme A thioesterase 4; Acyl-CoA thioesterase 4; EC 3.1.2.2; PTE-2b; Peroxisomal acyl coenzyme A thioester hydrolase Ib; Peroxisomal long-chain acyl-CoA thioesterase Ib; PTE-Ib
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSATLILEPPGRCCWNEPVRIAVRGLAPEQRVTLRASLRDEKGALFRAHARYCADARGEL
DLERAPALGGSFAGLEPMGLLWALEPEKPFWRFLKRDVQIPFVVELEVLDGHDPEPGRLL
CQAQHERHFLPPGVRRQSVRAGRVRATLFLPPGPGPFPGIIDIFGIGGGLLEYRASLLAG
HGFATLALAYYNFEDLPNNMDNISLEYFEEAVCYMLQHPQVKGPGIGLLGISLGADICLS
MASFLKNVSATVSINGSGISGNTAINYKHSSIPPLGYDLRRIKVAFSGLVDIVDIRNALV
GGYKNPSMIPIEKAQGPILLIVGQDDHNWRSELYAQTVSERLQAHGKEKPQIICYPGTGH
YIEPPYFPLCPASLHRLLNKHVIWGGEPRAHSKAQEDAWKQILAFFCKHLGGTQKTAVPK
L
Target Bioclass
Enzyme
Family
C/M/P thioester hydrolase family
Subcellular location
Peroxisome
Function
Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Functions as a peroxisomal succinyl-coenzyme A thioesterase that can also hydrolyze glutaryl-CoA and long chain saturated acyl-CoAs.
Uniprot ID
Q8N9L9
Ensemble ID
ENST00000326303.5
HGNC ID
HGNC:19748

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K380(1.82)  LDD0277  [1]
IPM
 Probe Info  		C14(0.00); C13(0.00)  LDD0241  [2]
AHL-Pu-1
 Probe Info  		C407(2.39)  LDD0170  [3]
IA-alkyne
 Probe Info  		C407(5.44)  LDD0513  [4]
DBIA
 Probe Info  		C407(0.95); C14(0.91)  LDD0078  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C407(2.39)  LDD0170  [3]
 LDCM0026  4SU-RNA+native RNA DM93 C407(3.27)  LDD0171  [3]
 LDCM0216  AC100 PaTu 8988t C407(0.74)  LDD1095  [5]
 LDCM0217  AC101 PaTu 8988t C407(0.84)  LDD1096  [5]
 LDCM0218  AC102 PaTu 8988t C407(1.50)  LDD1097  [5]
 LDCM0219  AC103 PaTu 8988t C407(1.23)  LDD1098  [5]
 LDCM0220  AC104 PaTu 8988t C407(0.85)  LDD1099  [5]
 LDCM0221  AC105 PaTu 8988t C407(0.83)  LDD1100  [5]
 LDCM0222  AC106 PaTu 8988t C407(1.19)  LDD1101  [5]
 LDCM0223  AC107 PaTu 8988t C407(0.98)  LDD1102  [5]
 LDCM0224  AC108 PaTu 8988t C407(1.07)  LDD1103  [5]
 LDCM0225  AC109 PaTu 8988t C407(1.05)  LDD1104  [5]
 LDCM0227  AC110 PaTu 8988t C407(1.22)  LDD1106  [5]
 LDCM0228  AC111 PaTu 8988t C407(1.31)  LDD1107  [5]
 LDCM0229  AC112 PaTu 8988t C407(0.94)  LDD1108  [5]
 LDCM0230  AC113 PaTu 8988t C407(0.83)  LDD1109  [5]
 LDCM0231  AC114 PaTu 8988t C407(0.82)  LDD1110  [5]
 LDCM0232  AC115 PaTu 8988t C407(0.83)  LDD1111  [5]
 LDCM0233  AC116 PaTu 8988t C407(0.88)  LDD1112  [5]
 LDCM0234  AC117 PaTu 8988t C407(0.77)  LDD1113  [5]
 LDCM0235  AC118 PaTu 8988t C407(0.92)  LDD1114  [5]
 LDCM0236  AC119 PaTu 8988t C407(0.93)  LDD1115  [5]
 LDCM0238  AC120 PaTu 8988t C407(0.91)  LDD1117  [5]
 LDCM0239  AC121 PaTu 8988t C407(0.89)  LDD1118  [5]
 LDCM0240  AC122 PaTu 8988t C407(1.13)  LDD1119  [5]
 LDCM0241  AC123 PaTu 8988t C407(1.15)  LDD1120  [5]
 LDCM0242  AC124 PaTu 8988t C407(1.20)  LDD1121  [5]
 LDCM0243  AC125 PaTu 8988t C407(1.31)  LDD1122  [5]
 LDCM0244  AC126 PaTu 8988t C407(1.01)  LDD1123  [5]
 LDCM0245  AC127 PaTu 8988t C407(1.02)  LDD1124  [5]
 LDCM0246  AC128 PaTu 8988t C407(0.91)  LDD1125  [5]
 LDCM0247  AC129 PaTu 8988t C407(1.08)  LDD1126  [5]
 LDCM0249  AC130 PaTu 8988t C407(0.98)  LDD1128  [5]
 LDCM0250  AC131 PaTu 8988t C407(1.11)  LDD1129  [5]
 LDCM0251  AC132 PaTu 8988t C407(1.01)  LDD1130  [5]
 LDCM0252  AC133 PaTu 8988t C407(1.15)  LDD1131  [5]
 LDCM0253  AC134 PaTu 8988t C407(1.09)  LDD1132  [5]
 LDCM0254  AC135 PaTu 8988t C407(1.16)  LDD1133  [5]
 LDCM0255  AC136 PaTu 8988t C407(0.95)  LDD1134  [5]
 LDCM0256  AC137 PaTu 8988t C407(1.07)  LDD1135  [5]
 LDCM0257  AC138 PaTu 8988t C407(1.01)  LDD1136  [5]
 LDCM0258  AC139 PaTu 8988t C407(0.87)  LDD1137  [5]
 LDCM0260  AC140 PaTu 8988t C407(0.93)  LDD1139  [5]
 LDCM0261  AC141 PaTu 8988t C407(1.02)  LDD1140  [5]
 LDCM0262  AC142 PaTu 8988t C407(1.06)  LDD1141  [5]
 LDCM0365  AC98 PaTu 8988t C407(1.23)  LDD1244  [5]
 LDCM0366  AC99 PaTu 8988t C407(0.99)  LDD1245  [5]
 LDCM0020  ARS-1620 HCC44 C407(0.95); C14(0.91)  LDD0078  [5]
 LDCM0420  CL31 PaTu 8988t C407(0.87)  LDD1299  [5]
 LDCM0421  CL32 PaTu 8988t C407(0.89)  LDD1300  [5]
 LDCM0422  CL33 PaTu 8988t C407(1.37)  LDD1301  [5]
 LDCM0423  CL34 PaTu 8988t C407(0.87)  LDD1302  [5]
 LDCM0424  CL35 PaTu 8988t C407(1.07)  LDD1303  [5]
 LDCM0425  CL36 PaTu 8988t C407(0.97)  LDD1304  [5]
 LDCM0426  CL37 PaTu 8988t C407(1.02)  LDD1305  [5]
 LDCM0428  CL39 PaTu 8988t C407(0.97)  LDD1307  [5]
 LDCM0430  CL40 PaTu 8988t C407(1.01)  LDD1309  [5]
 LDCM0431  CL41 PaTu 8988t C407(1.03)  LDD1310  [5]
 LDCM0432  CL42 PaTu 8988t C407(1.15)  LDD1311  [5]
 LDCM0433  CL43 PaTu 8988t C407(1.24)  LDD1312  [5]
 LDCM0434  CL44 PaTu 8988t C407(1.14)  LDD1313  [5]
 LDCM0435  CL45 PaTu 8988t C407(1.19)  LDD1314  [5]
 LDCM0436  CL46 PaTu 8988t C407(0.88)  LDD1315  [5]
 LDCM0437  CL47 PaTu 8988t C407(0.87)  LDD1316  [5]
 LDCM0438  CL48 PaTu 8988t C407(0.85)  LDD1317  [5]
 LDCM0439  CL49 PaTu 8988t C407(0.84)  LDD1318  [5]
 LDCM0441  CL50 PaTu 8988t C407(0.77)  LDD1320  [5]
 LDCM0442  CL51 PaTu 8988t C407(0.65)  LDD1321  [5]
 LDCM0443  CL52 PaTu 8988t C407(0.87)  LDD1322  [5]
 LDCM0444  CL53 PaTu 8988t C407(0.96)  LDD1323  [5]
 LDCM0445  CL54 PaTu 8988t C407(1.06)  LDD1324  [5]
 LDCM0446  CL55 PaTu 8988t C407(1.05)  LDD1325  [5]
 LDCM0447  CL56 PaTu 8988t C407(0.30)  LDD1326  [5]
 LDCM0448  CL57 PaTu 8988t C407(1.30)  LDD1327  [5]
 LDCM0449  CL58 PaTu 8988t C407(1.05)  LDD1328  [5]
 LDCM0450  CL59 PaTu 8988t C407(1.38)  LDD1329  [5]
 LDCM0452  CL60 PaTu 8988t C407(1.01)  LDD1331  [5]
 LDCM0453  CL61 PaTu 8988t C407(1.06)  LDD1332  [5]
 LDCM0454  CL62 PaTu 8988t C407(0.87)  LDD1333  [5]
 LDCM0455  CL63 PaTu 8988t C407(1.03)  LDD1334  [5]
 LDCM0456  CL64 PaTu 8988t C407(0.98)  LDD1335  [5]
 LDCM0457  CL65 PaTu 8988t C407(0.96)  LDD1336  [5]
 LDCM0458  CL66 PaTu 8988t C407(1.05)  LDD1337  [5]
 LDCM0459  CL67 PaTu 8988t C407(1.21)  LDD1338  [5]
 LDCM0460  CL68 PaTu 8988t C407(1.39)  LDD1339  [5]
 LDCM0461  CL69 PaTu 8988t C407(1.28)  LDD1340  [5]
 LDCM0463  CL70 PaTu 8988t C407(1.25)  LDD1342  [5]
 LDCM0464  CL71 PaTu 8988t C407(0.92)  LDD1343  [5]
 LDCM0465  CL72 PaTu 8988t C407(0.94)  LDD1344  [5]
 LDCM0466  CL73 PaTu 8988t C407(1.28)  LDD1345  [5]
 LDCM0467  CL74 PaTu 8988t C407(1.01)  LDD1346  [5]
 LDCM0198  Dimethyl Fumarate(DMF) T cell C407(5.44)  LDD0513  [4]
 LDCM0468  Fragment33 PaTu 8988t C407(1.09)  LDD1347  [5]
 LDCM0427  Fragment51 PaTu 8988t C407(1.19)  LDD1306  [5]
 LDCM0022  KB02 A431 C121(0.72)  LDD2258  [6]
 LDCM0023  KB03 A431 C121(0.80)  LDD2675  [6]
 LDCM0024  KB05 HMCB C121(1.13)  LDD3312  [6]

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Oxidant-Induced Bioconjugation for Protein Labeling in Live Cells. ACS Chem Biol. 2023 Jan 20;18(1):112-122. doi: 10.1021/acschembio.2c00740. Epub 2022 Dec 21.
3 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
4 An Activity-Guided Map of Electrophile-Cysteine Interactions in Primary Human T Cells. Cell. 2020 Aug 20;182(4):1009-1026.e29. doi: 10.1016/j.cell.2020.07.001. Epub 2020 Jul 29.
5 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
6 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840