Details of the Target

General Information of Target

Target ID LDTP08586
Target Name SMC5-SMC6 complex localization factor protein 2 (SLF2)
Gene Name SLF2
Gene ID 55719
Synonyms
C10orf6; FAM178A; SMC5-SMC6 complex localization factor protein 2; Smc5/6 localization factor 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MTRRCMPARPGFPSSPAPGSSPPRCHLRPGSTAHAAAGKRTESPGDRKQSIIDFFKPASK
QDRHMLDSPQKSNIKYGGSRLSITGTEQFERKLSSPKESKPKRVPPEKSPIIEAFMKGVK
EHHEDHGIHESRRPCLSLASKYLAKGTNIYVPSSYHLPKEMKSLKKKHRSPERRKSLFIH
ENNEKNDRDRGKTNADSKKQTTVAEADIFNNSSRSLSSRSSLSRHHPEESPLGAKFQLSL
ASYCRERELKRLRKEQMEQRINSENSFSEASSLSLKSSIERKYKPRQEQRKQNDIIPGKN
NLSNVENGHLSRKRSSSDSWEPTSAGSKQNKFPEKRKRNSVDSDLKSTRESMIPKARESF
LEKRPDGPHQKEKFIKHIALKTPGDVLRLEDISKEPSDETDGSSAGLAPSNSGNSGHHST
RNSDQIQVAGTKETKMQKPHLPLSQEKSAIKKASNLQKNKTASSTTKEKETKLPLLSRVP
SAGSSLVPLNAKNCALPVSKKDKERSSSKECSGHSTESTKHKEHKAKTNKADSNVSSGKI
SGGPLRSEYGTPTKSPPAALEVVPCIPSPAAPSDKAPSEGESSGNSNAGSSALKRKLRGD
FDSDEESLGYNLDSDEEEETLKSLEEIMALNFNQTPAATGKPPALSKGLRSQSSDYTGHV
HPGTYTNTLERLVKEMEDTQRLDELQKQLQEDIRQGRGIKSPIRIGEEDSTDDEDGLLEE
HKEFLKKFSVTIDAIPDHHPGEEIFNFLNSGKIFNQYTLDLRDSGFIGQSAVEKLILKSG
KTDQIFLTTQGFLTSAYHYVQCPVPVLKWLFRMMSVHTDCIVSVQILSTLMEITIRNDTF
SDSPVWPWIPSLSDVAAVFFNMGIDFRSLFPLENLQPDFNEDYLVSETQTTSRGKESEDS
SYKPIFSTLPETNILNVVKFLGLCTSIHPEGYQDREIMLLILMLFKMSLEKQLKQIPLVD
FQSLLINLMKNIRDWNTKVPELCLGINELSSHPHNLLWLVQLVPNWTSRGRQLRQCLSLV
IISKLLDEKHEDVPNASNLQVSVLHRYLVQMKPSDLLKKMVLKKKAEQPDGIIDDSLHLE
LEKQAYYLTYILLHLVGEVSCSHSFSSGQRKHFVLLCGALEKHVKCDIREDARLFYRTKV
KDLVARIHGKWQEIIQNCRPTQGQLHDFWVPDS
Target Bioclass
Other
Family
FAM178 family
Subcellular location
Nucleus
Function
Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks. Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions. Plays a role in SMC5-SMC6 complex recruitment for viral restriction. Forms a complex with SIMC1 and this complex is required to recruit SMC5-SMC6 complex to PML nuclear bodies and sites of viral replication.
Uniprot ID
Q8IX21
Ensemble ID
ENST00000238961.9
HGNC ID
HGNC:17814

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
A3KAW SNV: p.T2P .
COLO792 SNV: p.S1076R .
CORL23 SNV: p.D960G .
HCT15 SNV: p.E691Ter .
HGC27 SNV: p.P7T .
IM95 Deletion: p.Y883Ter .
LU65 SNV: p.P368S DBIA    Probe Info 
MCC13 SNV: p.N73K;p.K1083N
Substitution: p.P736F		 DBIA    Probe Info 
MOLT4 SNV: p.S537P .
RKO Deletion: p.P557LfsTer37 .
SNGM Insertion: p.L787FfsTer50 .
YSCCC SNV: p.L252F DBIA    Probe Info 

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 5 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C511(1.17)  LDD3342  [1]
AHL-Pu-1
 Probe Info  		C135(2.90)  LDD0170  [2]
IA-alkyne
 Probe Info  		N.A.  LDD0165  [3]
IPM
 Probe Info  		N.A.  LDD0005  [4]
Phosphinate-6
 Probe Info  		C511(0.00); C244(0.00)  LDD0018  [5]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0025  4SU-RNA DM93 C135(2.90)  LDD0170  [2]
 LDCM0026  4SU-RNA+native RNA DM93 C511(4.02); C135(3.01)  LDD0171  [2]
 LDCM0226  AC11 HEK-293T C511(0.89); C802(0.87)  LDD1509  [6]
 LDCM0237  AC12 HEK-293T C511(0.95)  LDD1510  [6]
 LDCM0259  AC14 HEK-293T C494(1.11); C1158(1.14)  LDD1512  [6]
 LDCM0270  AC15 HEK-293T C1117(1.21); C244(0.84)  LDD1513  [6]
 LDCM0278  AC19 HEK-293T C511(0.80); C802(0.86)  LDD1517  [6]
 LDCM0280  AC20 HEK-293T C511(0.90)  LDD1519  [6]
 LDCM0281  AC21 HEK-293T C511(0.93)  LDD1520  [6]
 LDCM0282  AC22 HEK-293T C494(0.98); C1158(1.04)  LDD1521  [6]
 LDCM0283  AC23 HEK-293T C1117(1.14); C244(0.98)  LDD1522  [6]
 LDCM0284  AC24 HEK-293T C511(0.95)  LDD1523  [6]
 LDCM0287  AC27 HEK-293T C511(1.09); C802(0.97)  LDD1526  [6]
 LDCM0288  AC28 HEK-293T C511(1.19)  LDD1527  [6]
 LDCM0289  AC29 HEK-293T C511(0.92)  LDD1528  [6]
 LDCM0290  AC3 HEK-293T C511(0.78); C802(1.11)  LDD1529  [6]
 LDCM0291  AC30 HEK-293T C494(0.96); C1158(0.93)  LDD1530  [6]
 LDCM0292  AC31 HEK-293T C1117(1.09); C244(0.95)  LDD1531  [6]
 LDCM0293  AC32 HEK-293T C511(0.95)  LDD1532  [6]
 LDCM0296  AC35 HEK-293T C511(1.01); C802(1.06)  LDD1535  [6]
 LDCM0297  AC36 HEK-293T C511(1.24)  LDD1536  [6]
 LDCM0298  AC37 HEK-293T C511(1.08)  LDD1537  [6]
 LDCM0299  AC38 HEK-293T C494(1.00); C1158(0.97)  LDD1538  [6]
 LDCM0300  AC39 HEK-293T C1117(1.18); C244(1.00)  LDD1539  [6]
 LDCM0301  AC4 HEK-293T C511(1.09)  LDD1540  [6]
 LDCM0302  AC40 HEK-293T C511(0.99)  LDD1541  [6]
 LDCM0305  AC43 HEK-293T C511(0.89); C802(0.91)  LDD1544  [6]
 LDCM0306  AC44 HEK-293T C511(0.84)  LDD1545  [6]
 LDCM0307  AC45 HEK-293T C511(0.93)  LDD1546  [6]
 LDCM0308  AC46 HEK-293T C494(1.24); C1158(1.04)  LDD1547  [6]
 LDCM0309  AC47 HEK-293T C1117(1.21); C244(0.99)  LDD1548  [6]
 LDCM0310  AC48 HEK-293T C511(1.01)  LDD1549  [6]
 LDCM0312  AC5 HEK-293T C511(0.93)  LDD1551  [6]
 LDCM0314  AC51 HEK-293T C511(0.90); C802(0.82)  LDD1553  [6]
 LDCM0315  AC52 HEK-293T C511(1.16)  LDD1554  [6]
 LDCM0316  AC53 HEK-293T C511(0.92)  LDD1555  [6]
 LDCM0317  AC54 HEK-293T C494(1.22); C1158(0.92)  LDD1556  [6]
 LDCM0318  AC55 HEK-293T C1117(1.08); C244(0.94)  LDD1557  [6]
 LDCM0319  AC56 HEK-293T C511(0.65)  LDD1558  [6]
 LDCM0322  AC59 HEK-293T C511(0.80); C802(0.87)  LDD1561  [6]
 LDCM0323  AC6 HEK-293T C494(1.29); C1158(0.80)  LDD1562  [6]
 LDCM0324  AC60 HEK-293T C511(1.09)  LDD1563  [6]
 LDCM0325  AC61 HEK-293T C511(0.82)  LDD1564  [6]
 LDCM0326  AC62 HEK-293T C494(0.95); C1158(1.01)  LDD1565  [6]
 LDCM0327  AC63 HEK-293T C1117(1.12); C244(1.04)  LDD1566  [6]
 LDCM0328  AC64 HEK-293T C511(0.87)  LDD1567  [6]
 LDCM0334  AC7 HEK-293T C1117(1.12); C244(0.88)  LDD1568  [6]
 LDCM0345  AC8 HEK-293T C511(0.98)  LDD1569  [6]
 LDCM0248  AKOS034007472 HEK-293T C511(0.89)  LDD1511  [6]
 LDCM0275  AKOS034007705 HEK-293T C511(0.99)  LDD1514  [6]
 LDCM0367  CL1 HEK-293T C511(1.00)  LDD1571  [6]
 LDCM0368  CL10 HEK-293T C494(1.25); C1158(0.87)  LDD1572  [6]
 LDCM0369  CL100 HEK-293T C494(1.04)  LDD1573  [6]
 LDCM0370  CL101 HEK-293T C511(0.94)  LDD1574  [6]
 LDCM0371  CL102 HEK-293T C511(1.00); C494(1.08)  LDD1575  [6]
 LDCM0373  CL104 HEK-293T C494(1.10)  LDD1577  [6]
 LDCM0374  CL105 HEK-293T C511(0.99)  LDD1578  [6]
 LDCM0375  CL106 HEK-293T C511(0.98); C494(1.00)  LDD1579  [6]
 LDCM0377  CL108 HEK-293T C494(0.98)  LDD1581  [6]
 LDCM0378  CL109 HEK-293T C511(1.03)  LDD1582  [6]
 LDCM0379  CL11 HEK-293T C1117(1.36); C244(0.83)  LDD1583  [6]
 LDCM0380  CL110 HEK-293T C511(0.99); C494(1.07)  LDD1584  [6]
 LDCM0382  CL112 HEK-293T C494(1.03)  LDD1586  [6]
 LDCM0383  CL113 HEK-293T C511(1.02)  LDD1587  [6]
 LDCM0384  CL114 HEK-293T C511(1.11); C494(0.89)  LDD1588  [6]
 LDCM0386  CL116 HEK-293T C494(1.06)  LDD1590  [6]
 LDCM0387  CL117 HEK-293T C511(0.88)  LDD1591  [6]
 LDCM0388  CL118 HEK-293T C511(0.95); C494(1.30)  LDD1592  [6]
 LDCM0390  CL12 HEK-293T C511(1.26)  LDD1594  [6]
 LDCM0391  CL120 HEK-293T C494(1.23)  LDD1595  [6]
 LDCM0392  CL121 HEK-293T C511(0.83)  LDD1596  [6]
 LDCM0393  CL122 HEK-293T C511(0.73); C494(1.10)  LDD1597  [6]
 LDCM0395  CL124 HEK-293T C494(1.13)  LDD1599  [6]
 LDCM0396  CL125 HEK-293T C511(0.53)  LDD1600  [6]
 LDCM0397  CL126 HEK-293T C511(0.72); C494(0.87)  LDD1601  [6]
 LDCM0399  CL128 HEK-293T C494(1.07)  LDD1603  [6]
 LDCM0400  CL13 HEK-293T C511(0.93)  LDD1604  [6]
 LDCM0401  CL14 HEK-293T C511(0.90); C494(0.99)  LDD1605  [6]
 LDCM0403  CL16 HEK-293T C494(1.04)  LDD1607  [6]
 LDCM0406  CL19 HEK-293T C511(0.77); C802(1.08)  LDD1610  [6]
 LDCM0407  CL2 HEK-293T C511(0.97); C494(1.02)  LDD1611  [6]
 LDCM0408  CL20 HEK-293T C511(1.06)  LDD1612  [6]
 LDCM0409  CL21 HEK-293T C511(0.94)  LDD1613  [6]
 LDCM0410  CL22 HEK-293T C494(1.15); C1158(0.97)  LDD1614  [6]
 LDCM0411  CL23 HEK-293T C1117(1.42); C244(0.97)  LDD1615  [6]
 LDCM0412  CL24 HEK-293T C511(0.92)  LDD1616  [6]
 LDCM0413  CL25 HEK-293T C511(0.75)  LDD1617  [6]
 LDCM0414  CL26 HEK-293T C511(0.86); C494(1.03)  LDD1618  [6]
 LDCM0416  CL28 HEK-293T C494(1.05)  LDD1620  [6]
 LDCM0420  CL31 HEK-293T C511(0.84); C802(1.00)  LDD1624  [6]
 LDCM0421  CL32 HEK-293T C511(0.84)  LDD1625  [6]
 LDCM0422  CL33 HEK-293T C511(0.89)  LDD1626  [6]
 LDCM0423  CL34 HEK-293T C494(1.17); C1158(0.85)  LDD1627  [6]
 LDCM0424  CL35 HEK-293T C1117(1.22); C244(0.88)  LDD1628  [6]
 LDCM0425  CL36 HEK-293T C511(0.92)  LDD1629  [6]
 LDCM0426  CL37 HEK-293T C511(0.81)  LDD1630  [6]
 LDCM0429  CL4 HEK-293T C494(1.11)  LDD1633  [6]
 LDCM0430  CL40 HEK-293T C494(0.98)  LDD1634  [6]
 LDCM0433  CL43 HEK-293T C511(0.78); C802(0.98)  LDD1637  [6]
 LDCM0434  CL44 HEK-293T C511(1.36)  LDD1638  [6]
 LDCM0435  CL45 HEK-293T C511(0.81)  LDD1639  [6]
 LDCM0436  CL46 HEK-293T C494(1.06); C1158(0.84)  LDD1640  [6]
 LDCM0437  CL47 HEK-293T C1117(1.09); C244(1.06)  LDD1641  [6]
 LDCM0438  CL48 HEK-293T C511(0.95)  LDD1642  [6]
 LDCM0439  CL49 HEK-293T C511(0.67)  LDD1643  [6]
 LDCM0441  CL50 HEK-293T C511(1.11); C494(1.01)  LDD1645  [6]
 LDCM0443  CL52 HEK-293T C494(1.20)  LDD1646  [6]
 LDCM0446  CL55 HEK-293T C511(0.89); C802(1.02)  LDD1649  [6]
 LDCM0447  CL56 HEK-293T C511(1.05)  LDD1650  [6]
 LDCM0448  CL57 HEK-293T C511(1.14)  LDD1651  [6]
 LDCM0449  CL58 HEK-293T C494(1.44); C1158(1.12)  LDD1652  [6]
 LDCM0450  CL59 HEK-293T C1117(1.23); C244(0.93)  LDD1653  [6]
 LDCM0452  CL60 HEK-293T C511(1.11)  LDD1655  [6]
 LDCM0453  CL61 HEK-293T C511(0.83)  LDD1656  [6]
 LDCM0454  CL62 HEK-293T C511(0.99); C494(0.95)  LDD1657  [6]
 LDCM0456  CL64 HEK-293T C494(1.03)  LDD1659  [6]
 LDCM0459  CL67 HEK-293T C511(0.71); C802(1.18)  LDD1662  [6]
 LDCM0460  CL68 HEK-293T C511(0.89)  LDD1663  [6]
 LDCM0461  CL69 HEK-293T C511(0.79)  LDD1664  [6]
 LDCM0462  CL7 HEK-293T C511(0.62); C802(1.07)  LDD1665  [6]
 LDCM0463  CL70 HEK-293T C494(1.25); C1158(1.00)  LDD1666  [6]
 LDCM0464  CL71 HEK-293T C1117(1.36); C244(1.08)  LDD1667  [6]
 LDCM0465  CL72 HEK-293T C511(0.95)  LDD1668  [6]
 LDCM0466  CL73 HEK-293T C511(0.73)  LDD1669  [6]
 LDCM0467  CL74 HEK-293T C511(0.94); C494(1.00)  LDD1670  [6]
 LDCM0469  CL76 HEK-293T C494(1.00)  LDD1672  [6]
 LDCM0472  CL79 HEK-293T C511(0.90); C802(0.86)  LDD1675  [6]
 LDCM0473  CL8 HEK-293T C511(0.96)  LDD1676  [6]
 LDCM0474  CL80 HEK-293T C511(0.97)  LDD1677  [6]
 LDCM0475  CL81 HEK-293T C511(0.74)  LDD1678  [6]
 LDCM0476  CL82 HEK-293T C494(1.06); C1158(1.08)  LDD1679  [6]
 LDCM0477  CL83 HEK-293T C1117(1.25); C244(0.97)  LDD1680  [6]
 LDCM0478  CL84 HEK-293T C511(1.13)  LDD1681  [6]
 LDCM0479  CL85 HEK-293T C511(0.64)  LDD1682  [6]
 LDCM0480  CL86 HEK-293T C511(0.70); C494(0.95)  LDD1683  [6]
 LDCM0482  CL88 HEK-293T C494(1.03)  LDD1685  [6]
 LDCM0484  CL9 HEK-293T C511(0.90)  LDD1687  [6]
 LDCM0486  CL91 HEK-293T C511(0.65); C802(0.87)  LDD1689  [6]
 LDCM0487  CL92 HEK-293T C511(0.93)  LDD1690  [6]
 LDCM0488  CL93 HEK-293T C511(0.80)  LDD1691  [6]
 LDCM0489  CL94 HEK-293T C494(1.05); C1158(0.98)  LDD1692  [6]
 LDCM0490  CL95 HEK-293T C1117(1.34); C244(1.07)  LDD1693  [6]
 LDCM0491  CL96 HEK-293T C511(0.64)  LDD1694  [6]
 LDCM0492  CL97 HEK-293T C511(0.89)  LDD1695  [6]
 LDCM0493  CL98 HEK-293T C511(1.07); C494(1.08)  LDD1696  [6]
 LDCM0427  Fragment51 HEK-293T C511(1.22); C494(1.06)  LDD1631  [6]
 LDCM0022  KB02 HEK-293T C511(0.99)  LDD1492  [6]
 LDCM0023  KB03 HEK-293T C511(0.86)  LDD1497  [6]
 LDCM0024  KB05 NCI-H1155 C511(1.17)  LDD3342  [1]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Proteasome subunit alpha type-1 (PSMA1) Peptidase T1A family P25786
Tripartite motif-containing protein 14 (TRIM14) TRIM/RBCC family Q14142
Other
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Structural maintenance of chromosomes protein 5 (SMC5) SMC family Q8IY18
Structural maintenance of chromosomes protein 6 (SMC6) SMC family Q96SB8
Scm-like with four MBT domains protein 2 (SFMBT2) . Q5VUG0

References

1 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840
2 Chemoproteomic capture of RNA binding activity in living cells. Nat Commun. 2023 Oct 7;14(1):6282. doi: 10.1038/s41467-023-41844-z.
Mass spectrometry data entry: PXD044625
3 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
4 A modification-centric assessment tool for the performance of chemoproteomic probes. Nat Chem Biol. 2022 Aug;18(8):904-912. doi: 10.1038/s41589-022-01074-8. Epub 2022 Jul 21.
Mass spectrometry data entry: PXD027758 , PXD027755 , PXD027760 , PXD027762 , PXD027756 , PXD027591 , PXD007149 , PXD030064 , PXD032392 , PXD027789 , PXD027767 , PXD027764
5 DFT-Guided Discovery of Ethynyl-Triazolyl-Phosphinates as Modular Electrophiles for Chemoselective Cysteine Bioconjugation and Profiling. Angew Chem Int Ed Engl. 2022 Oct 10;61(41):e202205348. doi: 10.1002/anie.202205348. Epub 2022 Aug 22.
Mass spectrometry data entry: PXD033004
6 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402