Details of the Target

General Information of Target

Target ID LDTP08522
Target Name Aprataxin and PNK-like factor (APLF)
Gene Name APLF
Gene ID 200558
Synonyms
C2orf13; PALF; XIP1; Aprataxin and PNK-like factor; EC 3.1.-.-; Apurinic-apyrimidinic endonuclease APLF; PNK and APTX-like FHA domain-containing protein; XRCC1-interacting protein 1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSGGFELQPRDGGPRVALAPGETVIGRGPLLGITDKRVSRRHAILEVAGGQLRIKPIHTN
PCFYQSSEKSQLLPLKPNLWCYLNPGDSFSLLVDKYIFRILSIPSEVEMQCTLRNSQVLD
EDNILNETPKSPVINLPHETTGASQLEGSTEIAKTQMTPTNSVSFLGENRDCNKQQPILA
ERKRILPTWMLAEHLSDQNLSVPAISGGNVIQGSGKEEICKDKSQLNTTQQGRRQLISSG
SSENTSAEQDTGEECKNTDQEESTISSKEMPQSFSAITLSNTEMNNIKTNAQRNKLPIEE
LGKVSKHKIATKRTPHKEDEAMSCSENCSSAQGDSLQDESQGSHSESSSNPSNPETLHAK
ATDSVLQGSEGNKVKRTSCMYGANCYRKNPVHFQHFSHPGDSDYGGVQIVGQDETDDRPE
CPYGPSCYRKNPQHKIEYRHNTLPVRNVLDEDNDNVGQPNEYDLNDSFLDDEEEDYEPTD
EDSDWEPGKEDEEKEDVEELLKEAKRFMKRK
Target Bioclass
Enzyme
Family
APLF family
Subcellular location
Nucleus
Function
Histone chaperone involved in single-strand and double-strand DNA break repair . Recruited to sites of DNA damage through interaction with branched poly-ADP-ribose chains, a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Following recruitment to DNA damage sites, acts as a histone chaperone that mediates histone eviction during DNA repair and promotes recruitment of histone variant MACROH2A1. Also has a nuclease activity: displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage. Together with PARP3, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ). Also acts as a negative regulator of cell pluripotency by promoting histone exchange. Required for the embryo implantation during the epithelial to mesenchymal transition in females.
Uniprot ID
Q8IW19
Ensemble ID
ENST00000303795.9
HGNC ID
HGNC:28724

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 2 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
IA-alkyne
 Probe Info  		C111(9.29)  LDD2157  [1]
DBIA
 Probe Info  		C111(1.27)  LDD1095  [2]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0216  AC100 PaTu 8988t C111(1.27)  LDD1095  [2]
 LDCM0217  AC101 PaTu 8988t C111(1.21)  LDD1096  [2]
 LDCM0218  AC102 PaTu 8988t C111(1.44)  LDD1097  [2]
 LDCM0219  AC103 PaTu 8988t C111(1.70)  LDD1098  [2]
 LDCM0220  AC104 PaTu 8988t C111(1.35)  LDD1099  [2]
 LDCM0221  AC105 PaTu 8988t C111(1.52)  LDD1100  [2]
 LDCM0222  AC106 PaTu 8988t C111(1.45)  LDD1101  [2]
 LDCM0223  AC107 PaTu 8988t C111(1.32)  LDD1102  [2]
 LDCM0224  AC108 PaTu 8988t C111(1.20)  LDD1103  [2]
 LDCM0225  AC109 PaTu 8988t C111(1.59)  LDD1104  [2]
 LDCM0227  AC110 PaTu 8988t C111(1.67)  LDD1106  [2]
 LDCM0228  AC111 PaTu 8988t C111(2.17)  LDD1107  [2]
 LDCM0229  AC112 PaTu 8988t C111(1.42)  LDD1108  [2]
 LDCM0230  AC113 PaTu 8988t C111(1.06)  LDD1109  [2]
 LDCM0231  AC114 PaTu 8988t C111(1.00)  LDD1110  [2]
 LDCM0232  AC115 PaTu 8988t C111(0.92)  LDD1111  [2]
 LDCM0233  AC116 PaTu 8988t C111(0.93)  LDD1112  [2]
 LDCM0234  AC117 PaTu 8988t C111(1.15)  LDD1113  [2]
 LDCM0235  AC118 PaTu 8988t C111(1.04)  LDD1114  [2]
 LDCM0236  AC119 PaTu 8988t C111(1.09)  LDD1115  [2]
 LDCM0238  AC120 PaTu 8988t C111(0.97)  LDD1117  [2]
 LDCM0239  AC121 PaTu 8988t C111(0.98)  LDD1118  [2]
 LDCM0240  AC122 PaTu 8988t C111(1.00)  LDD1119  [2]
 LDCM0241  AC123 PaTu 8988t C111(1.05)  LDD1120  [2]
 LDCM0242  AC124 PaTu 8988t C111(1.06)  LDD1121  [2]
 LDCM0243  AC125 PaTu 8988t C111(1.21)  LDD1122  [2]
 LDCM0244  AC126 PaTu 8988t C111(0.88)  LDD1123  [2]
 LDCM0245  AC127 PaTu 8988t C111(0.89)  LDD1124  [2]
 LDCM0246  AC128 PaTu 8988t C111(1.08)  LDD1125  [2]
 LDCM0247  AC129 PaTu 8988t C111(1.14)  LDD1126  [2]
 LDCM0249  AC130 PaTu 8988t C111(0.94)  LDD1128  [2]
 LDCM0250  AC131 PaTu 8988t C111(1.01)  LDD1129  [2]
 LDCM0251  AC132 PaTu 8988t C111(0.94)  LDD1130  [2]
 LDCM0252  AC133 PaTu 8988t C111(1.50)  LDD1131  [2]
 LDCM0253  AC134 PaTu 8988t C111(1.24)  LDD1132  [2]
 LDCM0254  AC135 PaTu 8988t C111(1.29)  LDD1133  [2]
 LDCM0255  AC136 PaTu 8988t C111(1.69)  LDD1134  [2]
 LDCM0256  AC137 PaTu 8988t C111(1.66)  LDD1135  [2]
 LDCM0257  AC138 PaTu 8988t C111(1.39)  LDD1136  [2]
 LDCM0258  AC139 PaTu 8988t C111(1.04)  LDD1137  [2]
 LDCM0260  AC140 PaTu 8988t C111(1.07)  LDD1139  [2]
 LDCM0261  AC141 PaTu 8988t C111(1.02)  LDD1140  [2]
 LDCM0262  AC142 PaTu 8988t C111(1.07)  LDD1141  [2]
 LDCM0263  AC143 PaTu 8988t C111(1.18)  LDD1142  [2]
 LDCM0264  AC144 PaTu 8988t C111(0.99)  LDD1143  [2]
 LDCM0265  AC145 PaTu 8988t C111(1.28)  LDD1144  [2]
 LDCM0266  AC146 PaTu 8988t C111(0.93)  LDD1145  [2]
 LDCM0267  AC147 PaTu 8988t C111(0.94)  LDD1146  [2]
 LDCM0268  AC148 PaTu 8988t C111(1.32)  LDD1147  [2]
 LDCM0269  AC149 PaTu 8988t C111(1.36)  LDD1148  [2]
 LDCM0271  AC150 PaTu 8988t C111(1.13)  LDD1150  [2]
 LDCM0272  AC151 PaTu 8988t C111(1.18)  LDD1151  [2]
 LDCM0273  AC152 PaTu 8988t C111(1.08)  LDD1152  [2]
 LDCM0274  AC153 PaTu 8988t C111(1.23)  LDD1153  [2]
 LDCM0621  AC154 PaTu 8988t C111(1.27)  LDD2166  [2]
 LDCM0622  AC155 PaTu 8988t C111(1.16)  LDD2167  [2]
 LDCM0623  AC156 PaTu 8988t C111(1.28)  LDD2168  [2]
 LDCM0624  AC157 PaTu 8988t C111(1.04)  LDD2169  [2]
 LDCM0276  AC17 PaTu 8988t C111(1.09)  LDD1155  [2]
 LDCM0277  AC18 PaTu 8988t C111(1.68)  LDD1156  [2]
 LDCM0278  AC19 PaTu 8988t C111(0.91)  LDD1157  [2]
 LDCM0280  AC20 PaTu 8988t C111(1.18)  LDD1159  [2]
 LDCM0281  AC21 PaTu 8988t C111(1.12)  LDD1160  [2]
 LDCM0282  AC22 PaTu 8988t C111(0.91)  LDD1161  [2]
 LDCM0283  AC23 PaTu 8988t C111(1.45)  LDD1162  [2]
 LDCM0284  AC24 PaTu 8988t C111(1.16)  LDD1163  [2]
 LDCM0308  AC46 PaTu 8988t C111(1.36)  LDD1187  [2]
 LDCM0309  AC47 PaTu 8988t C111(1.69)  LDD1188  [2]
 LDCM0310  AC48 PaTu 8988t C111(1.41)  LDD1189  [2]
 LDCM0311  AC49 PaTu 8988t C111(1.51)  LDD1190  [2]
 LDCM0313  AC50 PaTu 8988t C111(1.64)  LDD1192  [2]
 LDCM0314  AC51 PaTu 8988t C111(1.25)  LDD1193  [2]
 LDCM0315  AC52 PaTu 8988t C111(1.38)  LDD1194  [2]
 LDCM0316  AC53 PaTu 8988t C111(1.83)  LDD1195  [2]
 LDCM0317  AC54 PaTu 8988t C111(2.07)  LDD1196  [2]
 LDCM0318  AC55 PaTu 8988t C111(1.38)  LDD1197  [2]
 LDCM0319  AC56 PaTu 8988t C111(1.90)  LDD1198  [2]
 LDCM0320  AC57 PaTu 8988t C111(1.61)  LDD1199  [2]
 LDCM0321  AC58 PaTu 8988t C111(1.05)  LDD1200  [2]
 LDCM0322  AC59 PaTu 8988t C111(1.61)  LDD1201  [2]
 LDCM0324  AC60 PaTu 8988t C111(1.76)  LDD1203  [2]
 LDCM0325  AC61 PaTu 8988t C111(1.20)  LDD1204  [2]
 LDCM0326  AC62 PaTu 8988t C111(1.29)  LDD1205  [2]
 LDCM0327  AC63 PaTu 8988t C111(1.32)  LDD1206  [2]
 LDCM0328  AC64 PaTu 8988t C111(1.24)  LDD1207  [2]
 LDCM0329  AC65 PaTu 8988t C111(1.39)  LDD1208  [2]
 LDCM0330  AC66 PaTu 8988t C111(1.61)  LDD1209  [2]
 LDCM0331  AC67 PaTu 8988t C111(1.54)  LDD1210  [2]
 LDCM0332  AC68 PaTu 8988t C111(2.05)  LDD1211  [2]
 LDCM0333  AC69 PaTu 8988t C111(1.37)  LDD1212  [2]
 LDCM0335  AC70 PaTu 8988t C111(1.16)  LDD1214  [2]
 LDCM0336  AC71 PaTu 8988t C111(1.01)  LDD1215  [2]
 LDCM0337  AC72 PaTu 8988t C111(1.29)  LDD1216  [2]
 LDCM0338  AC73 PaTu 8988t C111(1.02)  LDD1217  [2]
 LDCM0339  AC74 PaTu 8988t C111(1.23)  LDD1218  [2]
 LDCM0340  AC75 PaTu 8988t C111(2.05)  LDD1219  [2]
 LDCM0341  AC76 PaTu 8988t C111(0.92)  LDD1220  [2]
 LDCM0342  AC77 PaTu 8988t C111(1.49)  LDD1221  [2]
 LDCM0343  AC78 PaTu 8988t C111(1.03)  LDD1222  [2]
 LDCM0344  AC79 PaTu 8988t C111(1.77)  LDD1223  [2]
 LDCM0346  AC80 PaTu 8988t C111(1.48)  LDD1225  [2]
 LDCM0347  AC81 PaTu 8988t C111(1.20)  LDD1226  [2]
 LDCM0348  AC82 PaTu 8988t C111(1.54)  LDD1227  [2]
 LDCM0349  AC83 PaTu 8988t C111(0.93)  LDD1228  [2]
 LDCM0350  AC84 PaTu 8988t C111(1.00)  LDD1229  [2]
 LDCM0351  AC85 PaTu 8988t C111(0.86)  LDD1230  [2]
 LDCM0352  AC86 PaTu 8988t C111(1.03)  LDD1231  [2]
 LDCM0353  AC87 PaTu 8988t C111(1.01)  LDD1232  [2]
 LDCM0354  AC88 PaTu 8988t C111(1.03)  LDD1233  [2]
 LDCM0355  AC89 PaTu 8988t C111(0.87)  LDD1234  [2]
 LDCM0357  AC90 PaTu 8988t C111(1.00)  LDD1236  [2]
 LDCM0358  AC91 PaTu 8988t C111(1.07)  LDD1237  [2]
 LDCM0359  AC92 PaTu 8988t C111(1.05)  LDD1238  [2]
 LDCM0360  AC93 PaTu 8988t C111(0.95)  LDD1239  [2]
 LDCM0361  AC94 PaTu 8988t C111(0.93)  LDD1240  [2]
 LDCM0362  AC95 PaTu 8988t C111(1.10)  LDD1241  [2]
 LDCM0363  AC96 PaTu 8988t C111(1.06)  LDD1242  [2]
 LDCM0364  AC97 PaTu 8988t C111(0.84)  LDD1243  [2]
 LDCM0365  AC98 PaTu 8988t C111(1.27)  LDD1244  [2]
 LDCM0366  AC99 PaTu 8988t C111(1.15)  LDD1245  [2]
 LDCM0367  CL1 PaTu 8988t C111(0.95)  LDD1246  [2]
 LDCM0368  CL10 PaTu 8988t C111(1.43)  LDD1247  [2]
 LDCM0374  CL105 PaTu 8988t C111(1.18)  LDD1253  [2]
 LDCM0375  CL106 PaTu 8988t C111(1.13)  LDD1254  [2]
 LDCM0376  CL107 PaTu 8988t C111(0.97)  LDD1255  [2]
 LDCM0377  CL108 PaTu 8988t C111(1.05)  LDD1256  [2]
 LDCM0378  CL109 PaTu 8988t C111(1.34)  LDD1257  [2]
 LDCM0379  CL11 PaTu 8988t C111(1.11)  LDD1258  [2]
 LDCM0380  CL110 PaTu 8988t C111(1.29)  LDD1259  [2]
 LDCM0381  CL111 PaTu 8988t C111(1.09)  LDD1260  [2]
 LDCM0390  CL12 PaTu 8988t C111(1.24)  LDD1269  [2]
 LDCM0392  CL121 PaTu 8988t C111(1.79)  LDD1271  [2]
 LDCM0393  CL122 PaTu 8988t C111(1.50)  LDD1272  [2]
 LDCM0394  CL123 PaTu 8988t C111(1.26)  LDD1273  [2]
 LDCM0395  CL124 PaTu 8988t C111(1.65)  LDD1274  [2]
 LDCM0396  CL125 PaTu 8988t C111(1.42)  LDD1275  [2]
 LDCM0397  CL126 PaTu 8988t C111(1.17)  LDD1276  [2]
 LDCM0398  CL127 PaTu 8988t C111(1.08)  LDD1277  [2]
 LDCM0399  CL128 PaTu 8988t C111(1.21)  LDD1278  [2]
 LDCM0400  CL13 PaTu 8988t C111(1.93)  LDD1279  [2]
 LDCM0401  CL14 PaTu 8988t C111(2.26)  LDD1280  [2]
 LDCM0402  CL15 PaTu 8988t C111(1.46)  LDD1281  [2]
 LDCM0407  CL2 PaTu 8988t C111(1.18)  LDD1286  [2]
 LDCM0418  CL3 PaTu 8988t C111(1.16)  LDD1297  [2]
 LDCM0420  CL31 PaTu 8988t C111(1.05)  LDD1299  [2]
 LDCM0421  CL32 PaTu 8988t C111(1.17)  LDD1300  [2]
 LDCM0422  CL33 PaTu 8988t C111(1.02)  LDD1301  [2]
 LDCM0423  CL34 PaTu 8988t C111(1.21)  LDD1302  [2]
 LDCM0424  CL35 PaTu 8988t C111(1.17)  LDD1303  [2]
 LDCM0425  CL36 PaTu 8988t C111(1.12)  LDD1304  [2]
 LDCM0426  CL37 PaTu 8988t C111(1.19)  LDD1305  [2]
 LDCM0428  CL39 PaTu 8988t C111(1.37)  LDD1307  [2]
 LDCM0429  CL4 PaTu 8988t C111(1.10)  LDD1308  [2]
 LDCM0430  CL40 PaTu 8988t C111(1.17)  LDD1309  [2]
 LDCM0431  CL41 PaTu 8988t C111(1.27)  LDD1310  [2]
 LDCM0432  CL42 PaTu 8988t C111(1.37)  LDD1311  [2]
 LDCM0433  CL43 PaTu 8988t C111(1.39)  LDD1312  [2]
 LDCM0434  CL44 PaTu 8988t C111(1.53)  LDD1313  [2]
 LDCM0435  CL45 PaTu 8988t C111(1.53)  LDD1314  [2]
 LDCM0436  CL46 PaTu 8988t C111(1.25)  LDD1315  [2]
 LDCM0437  CL47 PaTu 8988t C111(1.42)  LDD1316  [2]
 LDCM0438  CL48 PaTu 8988t C111(0.99)  LDD1317  [2]
 LDCM0439  CL49 PaTu 8988t C111(0.96)  LDD1318  [2]
 LDCM0440  CL5 PaTu 8988t C111(1.72)  LDD1319  [2]
 LDCM0441  CL50 PaTu 8988t C111(0.87)  LDD1320  [2]
 LDCM0442  CL51 PaTu 8988t C111(1.27)  LDD1321  [2]
 LDCM0443  CL52 PaTu 8988t C111(1.12)  LDD1322  [2]
 LDCM0444  CL53 PaTu 8988t C111(1.09)  LDD1323  [2]
 LDCM0445  CL54 PaTu 8988t C111(0.80)  LDD1324  [2]
 LDCM0446  CL55 PaTu 8988t C111(1.03)  LDD1325  [2]
 LDCM0447  CL56 PaTu 8988t C111(1.17)  LDD1326  [2]
 LDCM0448  CL57 PaTu 8988t C111(1.20)  LDD1327  [2]
 LDCM0449  CL58 PaTu 8988t C111(1.14)  LDD1328  [2]
 LDCM0450  CL59 PaTu 8988t C111(0.89)  LDD1329  [2]
 LDCM0451  CL6 PaTu 8988t C111(1.42)  LDD1330  [2]
 LDCM0452  CL60 PaTu 8988t C111(1.11)  LDD1331  [2]
 LDCM0453  CL61 PaTu 8988t C111(0.98)  LDD1332  [2]
 LDCM0454  CL62 PaTu 8988t C111(1.10)  LDD1333  [2]
 LDCM0455  CL63 PaTu 8988t C111(0.92)  LDD1334  [2]
 LDCM0456  CL64 PaTu 8988t C111(1.05)  LDD1335  [2]
 LDCM0457  CL65 PaTu 8988t C111(1.05)  LDD1336  [2]
 LDCM0458  CL66 PaTu 8988t C111(1.34)  LDD1337  [2]
 LDCM0459  CL67 PaTu 8988t C111(1.32)  LDD1338  [2]
 LDCM0460  CL68 PaTu 8988t C111(1.49)  LDD1339  [2]
 LDCM0461  CL69 PaTu 8988t C111(1.45)  LDD1340  [2]
 LDCM0462  CL7 PaTu 8988t C111(1.26)  LDD1341  [2]
 LDCM0463  CL70 PaTu 8988t C111(1.04)  LDD1342  [2]
 LDCM0464  CL71 PaTu 8988t C111(0.98)  LDD1343  [2]
 LDCM0465  CL72 PaTu 8988t C111(0.99)  LDD1344  [2]
 LDCM0466  CL73 PaTu 8988t C111(1.39)  LDD1345  [2]
 LDCM0467  CL74 PaTu 8988t C111(0.99)  LDD1346  [2]
 LDCM0469  CL76 PaTu 8988t C111(0.86)  LDD1348  [2]
 LDCM0470  CL77 PaTu 8988t C111(1.32)  LDD1349  [2]
 LDCM0471  CL78 PaTu 8988t C111(1.20)  LDD1350  [2]
 LDCM0472  CL79 PaTu 8988t C111(0.77)  LDD1351  [2]
 LDCM0473  CL8 PaTu 8988t C111(1.26)  LDD1352  [2]
 LDCM0474  CL80 PaTu 8988t C111(1.32)  LDD1353  [2]
 LDCM0475  CL81 PaTu 8988t C111(1.09)  LDD1354  [2]
 LDCM0476  CL82 PaTu 8988t C111(1.03)  LDD1355  [2]
 LDCM0477  CL83 PaTu 8988t C111(1.19)  LDD1356  [2]
 LDCM0478  CL84 PaTu 8988t C111(1.48)  LDD1357  [2]
 LDCM0479  CL85 PaTu 8988t C111(0.71)  LDD1358  [2]
 LDCM0480  CL86 PaTu 8988t C111(1.34)  LDD1359  [2]
 LDCM0481  CL87 PaTu 8988t C111(1.45)  LDD1360  [2]
 LDCM0482  CL88 PaTu 8988t C111(1.27)  LDD1361  [2]
 LDCM0483  CL89 PaTu 8988t C111(1.73)  LDD1362  [2]
 LDCM0484  CL9 PaTu 8988t C111(1.15)  LDD1363  [2]
 LDCM0485  CL90 PaTu 8988t C111(1.39)  LDD1364  [2]
 LDCM0468  Fragment33 PaTu 8988t C111(1.20)  LDD1347  [2]
 LDCM0427  Fragment51 PaTu 8988t C111(1.22)  LDD1306  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 3 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Poly [ADP-ribose] polymerase 1 (PARP1) ARTD/PARP family P09874
DNA ligase 4 (LIG4) ATP-dependent DNA ligase family P49917
X-ray repair cross-complementing protein 5 (XRCC5) Ku80 family P13010
Other
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
DNA repair protein XRCC4 (XRCC4) XRCC4-XLF family Q13426
DNA repair protein XRCC1 (XRCC1) . P18887

References

1 From chemoproteomic-detected amino acids to genomic coordinates: insights into precise multi-omic data integration. Mol Syst Biol. 2021 Feb;17(2):e9840. doi: 10.15252/msb.20209840.
Mass spectrometry data entry: PXD022151
2 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.