Details of the Target

General Information of Target

Target ID LDTP07877
Target Name Ubiquitin carboxyl-terminal hydrolase 31 (USP31)
Gene Name USP31
Gene ID 57478
Synonyms
KIAA1203; Ubiquitin carboxyl-terminal hydrolase 31; EC 3.4.19.12; Deubiquitinating enzyme 31; Ubiquitin thioesterase 31; Ubiquitin-specific-processing protease 31
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MSKVTAPGSGPPAAASGKEKRSFSKRLFRSGRAGGGGAGGPGASGPAAPSSPSSPSSARS
VGSFMSRVLKTLSTLSHLSSEGAAPDRGGLRSCFPPGPAAAPTPPPCPPPPASPAPPACA
AEPVPGVAGLRNHGNTCFMNATLQCLSNTELFAEYLALGQYRAGRPEPSPDPEQPAGRGA
QGQGEVTEQLAHLVRALWTLEYTPQHSRDFKTIVSKNALQYRGNSQHDAQEFLLWLLDRV
HEDLNHSVKQSGQPPLKPPSETDMMPEGPSFPVCSTFVQELFQAQYRSSLTCPHCQKQSN
TFDPFLCISLPIPLPHTRPLYVTVVYQGKCSHCMRIGVAVPLSGTVARLREAVSMETKIP
TDQIVLTEMYYDGFHRSFCDTDDLETVHESDCIFAFETPEIFRPEGILSQRGIHLNNNLN
HLKFGLDYHRLSSPTQTAAKQGKMDSPTSRAGSDKIVLLVCNRACTGQQGKRFGLPFVLH
LEKTIAWDLLQKEILEKMKYFLRPTVCIQVCPFSLRVVSVVGITYLLPQEEQPLCHPIVE
RALKSCGPGGTAHVKLVVEWDKETRDFLFVNTEDEYIPDAESVRLQRERHHQPQTCTLSQ
CFQLYTKEERLAPDDAWRCPHCKQLQQGSITLSLWTLPDVLIIHLKRFRQEGDRRMKLQN
MVKFPLTGLDMTPHVVKRSQSSWSLPSHWSPWRRPYGLGRDPEDYIYDLYAVCNHHGTMQ
GGHYTAYCKNSVDGLWYCFDDSDVQQLSEDEVCTQTAYILFYQRRTAIPSWSANSSVAGS
TSSSLCEHWVSRLPGSKPASVTSAASSRRTSLASLSESVEMTGERSEDDGGFSTRPFVRS
VQRQSLSSRSSVTSPLAVNENCMRPSWSLSAKLQMRSNSPSRFSGDSPIHSSASTLEKIG
EAADDKVSISCFGSLRNLSSSYQEPSDSHSRREHKAVGRAPLAVMEGVFKDESDTRRLNS
SVVDTQSKHSAQGDRLPPLSGPFDNNNQIAYVDQSDSVDSSPVKEVKAPSHPGSLAKKPE
STTKRSPSSKGTSEPEKSLRKGRPALASQESSLSSTSPSSPLPVKVSLKPSRSRSKADSS
SRGSGRHSSPAPAQPKKESSPKSQDSVSSPSPQKQKSASALTYTASSTSAKKASGPATRS
PFPPGKSRTSDHSLSREGSRQSLGSDRASATSTSKPNSPRVSQARAGEGRGAGKHVRSSS
MASLRSPSTSIKSGLKRDSKSEDKGLSFFKSALRQKETRRSTDLGKTALLSKKAGGSSVK
SVCKNTGDDEAERGHQPPASQQPNANTTGKEQLVTKDPASAKHSLLSARKSKSSQLDSGV
PSSPGGRQSAEKSSKKLSSSMQTSARPSQKPQ
Target Bioclass
Enzyme
Family
Peptidase C19 family
Function May recognize and hydrolyze the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.
Uniprot ID
Q70CQ4
Ensemble ID
ENST00000219689.12
HGNC ID
HGNC:20060

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
8505C SNV: p.L72M .
CHL1 SNV: p.P982S DBIA    Probe Info 
DETROIT562 SNV: p.S682T DBIA    Probe Info 
HCT15 SNV: p.S914R; p.A1137T DBIA    Probe Info 
HEC1 Insertion: p.G42RfsTer38
SNV: p.S1108P		 DBIA    Probe Info 
HEC1B SNV: p.S299C .
HGC27 Substitution: p.P111S .
HT115 SNV: p.D579N DBIA    Probe Info 
IM95 SNV: p.R792W .
JURKAT SNV: p.A726V .
MM1S SNV: p.P1092S .
RD SNV: p.S850Y DBIA    Probe Info 
RKN SNV: p.G697R .
RKO SNV: p.W789R DBIA    Probe Info 
SG231 SNV: p.S847P DBIA    Probe Info 
SW756 Deletion: p.P1095del .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C911(1.07)  LDD0533  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HEK-293T C465(0.97)  LDD1507  [2]
 LDCM0215  AC10 HEK-293T C465(1.14)  LDD1508  [2]
 LDCM0216  AC100 HCT 116 C911(1.07)  LDD0533  [1]
 LDCM0217  AC101 HCT 116 C911(1.08)  LDD0534  [1]
 LDCM0218  AC102 HCT 116 C911(1.16)  LDD0535  [1]
 LDCM0219  AC103 HCT 116 C911(1.64)  LDD0536  [1]
 LDCM0220  AC104 HCT 116 C911(1.40)  LDD0537  [1]
 LDCM0221  AC105 HCT 116 C911(1.25)  LDD0538  [1]
 LDCM0222  AC106 HCT 116 C911(1.48)  LDD0539  [1]
 LDCM0223  AC107 HCT 116 C911(1.44)  LDD0540  [1]
 LDCM0224  AC108 HCT 116 C911(1.03)  LDD0541  [1]
 LDCM0225  AC109 HCT 116 C911(0.96)  LDD0542  [1]
 LDCM0227  AC110 HCT 116 C911(1.07)  LDD0544  [1]
 LDCM0228  AC111 HCT 116 C911(0.93)  LDD0545  [1]
 LDCM0229  AC112 HCT 116 C911(1.29)  LDD0546  [1]
 LDCM0230  AC113 HCT 116 C911(0.86)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C911(1.17)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C911(1.20)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C911(1.37)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C911(1.09)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C911(0.92)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C911(0.91)  LDD0553  [1]
 LDCM0238  AC120 HCT 116 C911(1.11)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C911(0.92)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C911(0.93)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C911(0.99)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C911(1.13)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C911(1.09)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C911(1.36)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C911(1.24)  LDD0562  [1]
 LDCM0246  AC128 HCT 116 C911(0.90)  LDD0563  [1]
 LDCM0247  AC129 HCT 116 C911(0.94)  LDD0564  [1]
 LDCM0249  AC130 HCT 116 C911(1.20)  LDD0566  [1]
 LDCM0250  AC131 HCT 116 C911(0.84)  LDD0567  [1]
 LDCM0251  AC132 HCT 116 C911(1.18)  LDD0568  [1]
 LDCM0252  AC133 HCT 116 C911(0.84)  LDD0569  [1]
 LDCM0253  AC134 HCT 116 C911(1.04)  LDD0570  [1]
 LDCM0254  AC135 HCT 116 C911(0.91)  LDD0571  [1]
 LDCM0255  AC136 HCT 116 C911(1.00)  LDD0572  [1]
 LDCM0256  AC137 HCT 116 C911(0.87)  LDD0573  [1]
 LDCM0257  AC138 HCT 116 C911(0.86)  LDD0574  [1]
 LDCM0258  AC139 HCT 116 C911(1.21)  LDD0575  [1]
 LDCM0260  AC140 HCT 116 C911(1.06)  LDD0577  [1]
 LDCM0261  AC141 HCT 116 C911(1.22)  LDD0578  [1]
 LDCM0262  AC142 HCT 116 C911(1.08)  LDD0579  [1]
 LDCM0263  AC143 HCT 116 C911(0.91)  LDD0580  [1]
 LDCM0264  AC144 HCT 116 C911(1.13)  LDD0581  [1]
 LDCM0265  AC145 HCT 116 C911(0.84)  LDD0582  [1]
 LDCM0266  AC146 HCT 116 C911(1.16)  LDD0583  [1]
 LDCM0267  AC147 HCT 116 C911(1.09)  LDD0584  [1]
 LDCM0268  AC148 HCT 116 C911(1.55)  LDD0585  [1]
 LDCM0269  AC149 HCT 116 C911(1.27)  LDD0586  [1]
 LDCM0270  AC15 HEK-293T C465(1.25)  LDD1513  [2]
 LDCM0271  AC150 HCT 116 C911(0.98)  LDD0588  [1]
 LDCM0272  AC151 HCT 116 C911(0.91)  LDD0589  [1]
 LDCM0273  AC152 HCT 116 C911(1.20)  LDD0590  [1]
 LDCM0274  AC153 HCT 116 C911(1.72)  LDD0591  [1]
 LDCM0621  AC154 HCT 116 C911(1.10)  LDD2158  [1]
 LDCM0622  AC155 HCT 116 C911(1.12)  LDD2159  [1]
 LDCM0623  AC156 HCT 116 C911(1.00)  LDD2160  [1]
 LDCM0624  AC157 HCT 116 C911(1.06)  LDD2161  [1]
 LDCM0276  AC17 HEK-293T C465(1.00)  LDD1515  [2]
 LDCM0277  AC18 HEK-293T C465(0.99)  LDD1516  [2]
 LDCM0279  AC2 HEK-293T C465(1.07)  LDD1518  [2]
 LDCM0283  AC23 HEK-293T C465(1.04)  LDD1522  [2]
 LDCM0285  AC25 HEK-293T C465(1.04)  LDD1524  [2]
 LDCM0286  AC26 HEK-293T C465(1.01)  LDD1525  [2]
 LDCM0292  AC31 HEK-293T C465(1.02)  LDD1531  [2]
 LDCM0294  AC33 HEK-293T C465(1.11)  LDD1533  [2]
 LDCM0295  AC34 HEK-293T C465(1.21)  LDD1534  [2]
 LDCM0300  AC39 HEK-293T C465(1.05)  LDD1539  [2]
 LDCM0303  AC41 HEK-293T C465(1.10)  LDD1542  [2]
 LDCM0304  AC42 HEK-293T C465(0.99)  LDD1543  [2]
 LDCM0308  AC46 PaTu 8988t C546(1.29)  LDD1187  [1]
 LDCM0309  AC47 PaTu 8988t C546(1.80)  LDD1188  [1]
 LDCM0310  AC48 PaTu 8988t C546(1.17)  LDD1189  [1]
 LDCM0311  AC49 PaTu 8988t C546(2.06)  LDD1190  [1]
 LDCM0313  AC50 PaTu 8988t C546(1.99)  LDD1192  [1]
 LDCM0314  AC51 PaTu 8988t C546(0.77)  LDD1193  [1]
 LDCM0315  AC52 PaTu 8988t C546(1.65)  LDD1194  [1]
 LDCM0316  AC53 PaTu 8988t C546(2.50)  LDD1195  [1]
 LDCM0317  AC54 PaTu 8988t C546(2.23)  LDD1196  [1]
 LDCM0318  AC55 PaTu 8988t C546(1.87)  LDD1197  [1]
 LDCM0319  AC56 PaTu 8988t C546(1.65)  LDD1198  [1]
 LDCM0320  AC57 HEK-293T C465(1.01)  LDD1559  [2]
 LDCM0321  AC58 HEK-293T C465(1.12)  LDD1560  [2]
 LDCM0327  AC63 HEK-293T C465(1.02)  LDD1566  [2]
 LDCM0332  AC68 HCT 116 C911(0.88)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C911(1.08)  LDD0650  [1]
 LDCM0334  AC7 HEK-293T C465(0.95)  LDD1568  [2]
 LDCM0335  AC70 HCT 116 C911(1.09)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C911(0.84)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C911(1.26)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C911(1.77)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C911(1.43)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C911(1.65)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C911(1.29)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C911(1.14)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C911(0.97)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C911(1.29)  LDD0661  [1]
 LDCM0346  AC80 HCT 116 C911(1.18)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C911(0.84)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C911(1.40)  LDD0665  [1]
 LDCM0349  AC83 HCT 116 C911(1.68)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C911(1.46)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C911(1.03)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C911(1.26)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C911(1.40)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C911(1.41)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C911(1.55)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C911(0.83)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C911(1.79)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C911(1.67)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C911(1.17)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C911(1.42)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C911(1.16)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C911(1.51)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C911(1.64)  LDD0681  [1]
 LDCM0365  AC98 HCT 116 C911(1.74)  LDD0682  [1]
 LDCM0366  AC99 HCT 116 C911(0.99)  LDD0683  [1]
 LDCM0356  AKOS034007680 HEK-293T C465(1.13)  LDD1570  [2]
 LDCM0372  CL103 HEK-293T C465(1.10)  LDD1576  [2]
 LDCM0376  CL107 HEK-293T C465(0.98)  LDD1580  [2]
 LDCM0379  CL11 HEK-293T C465(1.35)  LDD1583  [2]
 LDCM0381  CL111 HEK-293T C465(1.03)  LDD1585  [2]
 LDCM0385  CL115 HEK-293T C465(1.06)  LDD1589  [2]
 LDCM0389  CL119 HEK-293T C465(1.10)  LDD1593  [2]
 LDCM0392  CL121 PaTu 8988t C546(1.43)  LDD1271  [1]
 LDCM0393  CL122 PaTu 8988t C546(1.17)  LDD1272  [1]
 LDCM0394  CL123 PaTu 8988t C546(1.55)  LDD1273  [1]
 LDCM0395  CL124 PaTu 8988t C546(1.33)  LDD1274  [1]
 LDCM0398  CL127 HEK-293T C465(1.13)  LDD1602  [2]
 LDCM0402  CL15 HEK-293T C465(0.86)  LDD1606  [2]
 LDCM0404  CL17 HEK-293T C465(0.98)  LDD1608  [2]
 LDCM0405  CL18 HEK-293T C465(1.09)  LDD1609  [2]
 LDCM0411  CL23 HEK-293T C465(1.26)  LDD1615  [2]
 LDCM0415  CL27 HEK-293T C465(0.99)  LDD1619  [2]
 LDCM0417  CL29 HEK-293T C465(1.05)  LDD1621  [2]
 LDCM0418  CL3 HEK-293T C465(1.09)  LDD1622  [2]
 LDCM0419  CL30 HEK-293T C465(1.39)  LDD1623  [2]
 LDCM0420  CL31 PaTu 8988t C546(1.02)  LDD1299  [1]
 LDCM0421  CL32 PaTu 8988t C546(1.52)  LDD1300  [1]
 LDCM0422  CL33 PaTu 8988t C546(1.29)  LDD1301  [1]
 LDCM0423  CL34 PaTu 8988t C546(1.79)  LDD1302  [1]
 LDCM0424  CL35 PaTu 8988t C546(1.17)  LDD1303  [1]
 LDCM0425  CL36 PaTu 8988t C546(0.38)  LDD1304  [1]
 LDCM0426  CL37 PaTu 8988t C546(1.34)  LDD1305  [1]
 LDCM0428  CL39 PaTu 8988t C546(1.55)  LDD1307  [1]
 LDCM0430  CL40 PaTu 8988t C546(1.18)  LDD1309  [1]
 LDCM0431  CL41 PaTu 8988t C546(1.14)  LDD1310  [1]
 LDCM0432  CL42 PaTu 8988t C546(1.18)  LDD1311  [1]
 LDCM0433  CL43 PaTu 8988t C546(1.28)  LDD1312  [1]
 LDCM0434  CL44 PaTu 8988t C546(1.40)  LDD1313  [1]
 LDCM0435  CL45 PaTu 8988t C546(1.50)  LDD1314  [1]
 LDCM0437  CL47 HEK-293T C465(1.23)  LDD1641  [2]
 LDCM0440  CL5 HEK-293T C465(0.94)  LDD1644  [2]
 LDCM0444  CL53 HEK-293T C465(1.23)  LDD1647  [2]
 LDCM0445  CL54 HEK-293T C465(0.88)  LDD1648  [2]
 LDCM0450  CL59 HEK-293T C465(1.38)  LDD1653  [2]
 LDCM0451  CL6 HEK-293T C465(0.98)  LDD1654  [2]
 LDCM0453  CL61 PaTu 8988t C546(1.06)  LDD1332  [1]
 LDCM0454  CL62 PaTu 8988t C546(0.98)  LDD1333  [1]
 LDCM0455  CL63 PaTu 8988t C546(1.16)  LDD1334  [1]
 LDCM0456  CL64 PaTu 8988t C546(1.22)  LDD1335  [1]
 LDCM0457  CL65 PaTu 8988t C546(1.40)  LDD1336  [1]
 LDCM0458  CL66 PaTu 8988t C546(1.63)  LDD1337  [1]
 LDCM0459  CL67 PaTu 8988t C546(1.56)  LDD1338  [1]
 LDCM0460  CL68 PaTu 8988t C546(1.75)  LDD1339  [1]
 LDCM0461  CL69 PaTu 8988t C546(1.64)  LDD1340  [1]
 LDCM0463  CL70 PaTu 8988t C546(1.44)  LDD1342  [1]
 LDCM0464  CL71 PaTu 8988t C546(0.74)  LDD1343  [1]
 LDCM0465  CL72 PaTu 8988t C546(0.69)  LDD1344  [1]
 LDCM0466  CL73 PaTu 8988t C546(1.81)  LDD1345  [1]
 LDCM0467  CL74 PaTu 8988t C546(0.92)  LDD1346  [1]
 LDCM0469  CL76 HCT 116 C911(0.77)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C911(0.77)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C911(0.82)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C911(0.82)  LDD0789  [1]
 LDCM0474  CL80 HCT 116 C911(0.71)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C911(0.84)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C911(0.90)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C911(0.87)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C911(0.90)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C911(0.85)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C911(0.89)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C911(0.81)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C911(0.83)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C911(0.88)  LDD0800  [1]
 LDCM0485  CL90 HCT 116 C911(0.89)  LDD0802  [1]
 LDCM0490  CL95 HEK-293T C465(1.06)  LDD1693  [2]
 LDCM0494  CL99 HEK-293T C465(1.16)  LDD1697  [2]
 LDCM0495  E2913 HEK-293T C465(1.13)  LDD1698  [2]
 LDCM0468  Fragment33 PaTu 8988t C546(1.21)  LDD1347  [1]
 LDCM0427  Fragment51 PaTu 8988t C546(1.89)  LDD1306  [1]
 LDCM0022  KB02 HEK-293T C465(1.41)  LDD1492  [2]
 LDCM0023  KB03 HEK-293T C465(1.27)  LDD1497  [2]
 LDCM0024  KB05 MEL167 C465(1.80)  LDD3316  [3]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402
3 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840