Details of the Target

General Information of Target

Target ID LDTP07463
Target Name Ultra-long-chain fatty acid omega-hydroxylase (CYP4F22)
Gene Name CYP4F22
Gene ID 126410
Synonyms
Ultra-long-chain fatty acid omega-hydroxylase; EC 1.14.14.177; Cytochrome P450 4F22
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MLPITDRLLHLLGLEKTAFRIYAVSTLLLFLLFFLFRLLLRFLRLCRSFYITCRRLRCFP
QPPRRNWLLGHLGMYLPNEAGLQDEKKVLDNMHHVLLVWMGPVLPLLVLVHPDYIKPLLG
ASAAIAPKDDLFYGFLKPWLGDGLLLSKGDKWSRHRRLLTPAFHFDILKPYMKIFNQSAD
IMHAKWRHLAEGSAVSLDMFEHISLMTLDSLQKCVFSYNSNCQEKMSDYISAIIELSALS
VRRQYRLHHYLDFIYYRSADGRRFRQACDMVHHFTTEVIQERRRALRQQGAEAWLKAKQG
KTLDFIDVLLLARDEDGKELSDEDIRAEADTFMFEGHDTTSSGISWMLFNLAKYPEYQEK
CREEIQEVMKGRELEELEWDDLTQLPFTTMCIKESLRQYPPVTLVSRQCTEDIKLPDGRI
IPKGIICLVSIYGTHHNPTVWPDSKVYNPYRFDPDNPQQRSPLAYVPFSAGPRNCIGQSF
AMAELRVVVALTLLRFRLSVDRTRKVRRKPELILRTENGLWLKVEPLPPRA
Target Bioclass
Enzyme
Family
Cytochrome P450 family
Subcellular location
Endoplasmic reticulum membrane
Function
A cytochrome P450 monooxygenase involved in epidermal ceramide biosynthesis. Hydroxylates the terminal carbon (omega-hydroxylation) of ultra-long-chain fatty acyls (C28-C36) prior to ceramide synthesis. Contributes to the synthesis of three classes of omega-hydroxy-ultra-long chain fatty acylceramides having sphingosine, 6-hydroxysphingosine and phytosphingosine bases, all major lipid components that underlie the permeability barrier of the stratum corneum. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Uniprot ID
Q6NT55
Ensemble ID
ENST00000269703.8
HGNC ID
HGNC:26820

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 1 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C214(0.73); C222(0.73)  LDD0531  [1]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C214(0.73); C222(0.73)  LDD0531  [1]
 LDCM0230  AC113 HCT 116 C214(0.76); C222(0.76)  LDD0547  [1]
 LDCM0231  AC114 HCT 116 C214(0.77); C222(0.77)  LDD0548  [1]
 LDCM0232  AC115 HCT 116 C214(0.73); C222(0.73)  LDD0549  [1]
 LDCM0233  AC116 HCT 116 C214(0.90); C222(0.90)  LDD0550  [1]
 LDCM0234  AC117 HCT 116 C214(0.75); C222(0.75)  LDD0551  [1]
 LDCM0235  AC118 HCT 116 C214(0.89); C222(0.89)  LDD0552  [1]
 LDCM0236  AC119 HCT 116 C214(0.74); C222(0.74)  LDD0553  [1]
 LDCM0238  AC120 HCT 116 C214(0.65); C222(0.65)  LDD0555  [1]
 LDCM0239  AC121 HCT 116 C214(1.09); C222(1.09)  LDD0556  [1]
 LDCM0240  AC122 HCT 116 C214(0.82); C222(0.82)  LDD0557  [1]
 LDCM0241  AC123 HCT 116 C214(0.98); C222(0.98)  LDD0558  [1]
 LDCM0242  AC124 HCT 116 C214(0.90); C222(0.90)  LDD0559  [1]
 LDCM0243  AC125 HCT 116 C214(0.79); C222(0.79)  LDD0560  [1]
 LDCM0244  AC126 HCT 116 C214(0.87); C222(0.87)  LDD0561  [1]
 LDCM0245  AC127 HCT 116 C214(0.86); C222(0.86)  LDD0562  [1]
 LDCM0276  AC17 HCT 116 C214(0.96); C222(0.96)  LDD0593  [1]
 LDCM0277  AC18 HCT 116 C214(0.88); C222(0.88)  LDD0594  [1]
 LDCM0278  AC19 HCT 116 C214(1.07); C222(1.07)  LDD0595  [1]
 LDCM0279  AC2 HCT 116 C214(0.78); C222(0.78)  LDD0596  [1]
 LDCM0280  AC20 HCT 116 C214(1.05); C222(1.05)  LDD0597  [1]
 LDCM0281  AC21 HCT 116 C214(0.93); C222(0.93)  LDD0598  [1]
 LDCM0282  AC22 HCT 116 C214(1.16); C222(1.16)  LDD0599  [1]
 LDCM0283  AC23 HCT 116 C214(1.16); C222(1.16)  LDD0600  [1]
 LDCM0284  AC24 HCT 116 C214(1.07); C222(1.07)  LDD0601  [1]
 LDCM0290  AC3 HCT 116 C214(0.85); C222(0.85)  LDD0607  [1]
 LDCM0296  AC35 HCT 116 C214(0.93); C222(0.93)  LDD0613  [1]
 LDCM0297  AC36 HCT 116 C214(0.79); C222(0.79)  LDD0614  [1]
 LDCM0298  AC37 HCT 116 C214(0.99); C222(0.99)  LDD0615  [1]
 LDCM0299  AC38 HCT 116 C214(0.91); C222(0.91)  LDD0616  [1]
 LDCM0300  AC39 HCT 116 C214(0.91); C222(0.91)  LDD0617  [1]
 LDCM0301  AC4 HCT 116 C214(0.99); C222(0.99)  LDD0618  [1]
 LDCM0302  AC40 HCT 116 C214(0.95); C222(0.95)  LDD0619  [1]
 LDCM0303  AC41 HCT 116 C214(0.70); C222(0.70)  LDD0620  [1]
 LDCM0304  AC42 HCT 116 C214(0.83); C222(0.83)  LDD0621  [1]
 LDCM0305  AC43 HCT 116 C214(0.71); C222(0.71)  LDD0622  [1]
 LDCM0306  AC44 HCT 116 C214(0.88); C222(0.88)  LDD0623  [1]
 LDCM0307  AC45 HCT 116 C214(1.01); C222(1.01)  LDD0624  [1]
 LDCM0308  AC46 HCT 116 C214(0.95); C222(0.95)  LDD0625  [1]
 LDCM0309  AC47 HCT 116 C214(0.86); C222(0.86)  LDD0626  [1]
 LDCM0310  AC48 HCT 116 C214(0.93); C222(0.93)  LDD0627  [1]
 LDCM0311  AC49 HCT 116 C214(1.32); C222(1.32)  LDD0628  [1]
 LDCM0312  AC5 HCT 116 C214(0.90); C222(0.90)  LDD0629  [1]
 LDCM0313  AC50 HCT 116 C214(1.23); C222(1.23)  LDD0630  [1]
 LDCM0314  AC51 HCT 116 C214(1.09); C222(1.09)  LDD0631  [1]
 LDCM0315  AC52 HCT 116 C214(0.83); C222(0.83)  LDD0632  [1]
 LDCM0316  AC53 HCT 116 C214(1.11); C222(1.11)  LDD0633  [1]
 LDCM0317  AC54 HCT 116 C214(1.04); C222(1.04)  LDD0634  [1]
 LDCM0318  AC55 HCT 116 C214(1.25); C222(1.25)  LDD0635  [1]
 LDCM0319  AC56 HCT 116 C214(0.98); C222(0.98)  LDD0636  [1]
 LDCM0320  AC57 HCT 116 C214(1.00); C222(1.00)  LDD0637  [1]
 LDCM0321  AC58 HCT 116 C214(1.05); C222(1.05)  LDD0638  [1]
 LDCM0322  AC59 HCT 116 C214(0.96); C222(0.96)  LDD0639  [1]
 LDCM0324  AC60 HCT 116 C214(0.90); C222(0.90)  LDD0641  [1]
 LDCM0325  AC61 HCT 116 C214(1.19); C222(1.19)  LDD0642  [1]
 LDCM0326  AC62 HCT 116 C214(0.81); C222(0.81)  LDD0643  [1]
 LDCM0327  AC63 HCT 116 C214(0.85); C222(0.85)  LDD0644  [1]
 LDCM0328  AC64 HCT 116 C214(0.94); C222(0.94)  LDD0645  [1]
 LDCM0329  AC65 HCT 116 C214(0.92); C222(0.92)  LDD0646  [1]
 LDCM0330  AC66 HCT 116 C214(1.00); C222(1.00)  LDD0647  [1]
 LDCM0331  AC67 HCT 116 C214(0.86); C222(0.86)  LDD0648  [1]
 LDCM0332  AC68 HCT 116 C214(0.86); C222(0.86)  LDD0649  [1]
 LDCM0333  AC69 HCT 116 C214(0.83); C222(0.83)  LDD0650  [1]
 LDCM0335  AC70 HCT 116 C214(0.78); C222(0.78)  LDD0652  [1]
 LDCM0336  AC71 HCT 116 C214(1.01); C222(1.01)  LDD0653  [1]
 LDCM0337  AC72 HCT 116 C214(0.94); C222(0.94)  LDD0654  [1]
 LDCM0338  AC73 HCT 116 C214(0.83); C222(0.83)  LDD0655  [1]
 LDCM0339  AC74 HCT 116 C214(0.83); C222(0.83)  LDD0656  [1]
 LDCM0340  AC75 HCT 116 C214(0.75); C222(0.75)  LDD0657  [1]
 LDCM0341  AC76 HCT 116 C214(0.92); C222(0.92)  LDD0658  [1]
 LDCM0342  AC77 HCT 116 C214(0.84); C222(0.84)  LDD0659  [1]
 LDCM0343  AC78 HCT 116 C214(0.81); C222(0.81)  LDD0660  [1]
 LDCM0344  AC79 HCT 116 C214(1.12); C222(1.12)  LDD0661  [1]
 LDCM0346  AC80 HCT 116 C214(0.94); C222(0.94)  LDD0663  [1]
 LDCM0347  AC81 HCT 116 C214(0.95); C222(0.95)  LDD0664  [1]
 LDCM0348  AC82 HCT 116 C214(0.70); C222(0.70)  LDD0665  [1]
 LDCM0349  AC83 HCT 116 C214(0.97); C222(0.97)  LDD0666  [1]
 LDCM0350  AC84 HCT 116 C214(0.88); C222(0.88)  LDD0667  [1]
 LDCM0351  AC85 HCT 116 C214(0.92); C222(0.92)  LDD0668  [1]
 LDCM0352  AC86 HCT 116 C214(0.91); C222(0.91)  LDD0669  [1]
 LDCM0353  AC87 HCT 116 C214(1.12); C222(1.12)  LDD0670  [1]
 LDCM0354  AC88 HCT 116 C214(1.12); C222(1.12)  LDD0671  [1]
 LDCM0355  AC89 HCT 116 C214(0.91); C222(0.91)  LDD0672  [1]
 LDCM0357  AC90 HCT 116 C214(1.13); C222(1.13)  LDD0674  [1]
 LDCM0358  AC91 HCT 116 C214(0.86); C222(0.86)  LDD0675  [1]
 LDCM0359  AC92 HCT 116 C214(0.96); C222(0.96)  LDD0676  [1]
 LDCM0360  AC93 HCT 116 C214(0.88); C222(0.88)  LDD0677  [1]
 LDCM0361  AC94 HCT 116 C214(0.94); C222(0.94)  LDD0678  [1]
 LDCM0362  AC95 HCT 116 C214(1.07); C222(1.07)  LDD0679  [1]
 LDCM0363  AC96 HCT 116 C214(0.98); C222(0.98)  LDD0680  [1]
 LDCM0364  AC97 HCT 116 C214(0.81); C222(0.81)  LDD0681  [1]
 LDCM0369  CL100 HCT 116 C214(0.78); C222(0.78)  LDD0686  [1]
 LDCM0374  CL105 HCT 116 C214(0.98); C222(0.98)  LDD0691  [1]
 LDCM0375  CL106 HCT 116 C214(0.57); C222(0.57)  LDD0692  [1]
 LDCM0376  CL107 HCT 116 C214(0.84); C222(0.84)  LDD0693  [1]
 LDCM0377  CL108 HCT 116 C214(0.87); C222(0.87)  LDD0694  [1]
 LDCM0378  CL109 HCT 116 C214(0.99); C222(0.99)  LDD0695  [1]
 LDCM0380  CL110 HCT 116 C214(0.99); C222(0.99)  LDD0697  [1]
 LDCM0381  CL111 HCT 116 C214(1.08); C222(1.08)  LDD0698  [1]
 LDCM0387  CL117 HCT 116 C214(0.91); C222(0.91)  LDD0704  [1]
 LDCM0388  CL118 HCT 116 C214(0.84); C222(0.84)  LDD0705  [1]
 LDCM0389  CL119 HCT 116 C214(0.75); C222(0.75)  LDD0706  [1]
 LDCM0391  CL120 HCT 116 C214(0.73); C222(0.73)  LDD0708  [1]
 LDCM0392  CL121 HCT 116 C214(1.24); C222(1.24)  LDD0709  [1]
 LDCM0393  CL122 HCT 116 C214(1.28); C222(1.28)  LDD0710  [1]
 LDCM0394  CL123 HCT 116 C214(1.14); C222(1.14)  LDD0711  [1]
 LDCM0395  CL124 HCT 116 C214(1.06); C222(1.06)  LDD0712  [1]
 LDCM0396  CL125 HCT 116 C214(0.96); C222(0.96)  LDD0713  [1]
 LDCM0397  CL126 HCT 116 C214(0.97); C222(0.97)  LDD0714  [1]
 LDCM0398  CL127 HCT 116 C214(0.94); C222(0.94)  LDD0715  [1]
 LDCM0399  CL128 HCT 116 C214(1.32); C222(1.32)  LDD0716  [1]
 LDCM0403  CL16 HCT 116 C214(1.03); C222(1.03)  LDD0720  [1]
 LDCM0404  CL17 HCT 116 C214(1.04); C222(1.04)  LDD0721  [1]
 LDCM0405  CL18 HCT 116 C214(0.81); C222(0.81)  LDD0722  [1]
 LDCM0406  CL19 HCT 116 C214(0.92); C222(0.92)  LDD0723  [1]
 LDCM0408  CL20 HCT 116 C214(0.79); C222(0.79)  LDD0725  [1]
 LDCM0409  CL21 HCT 116 C214(0.98); C222(0.98)  LDD0726  [1]
 LDCM0410  CL22 HCT 116 C214(0.82); C222(0.82)  LDD0727  [1]
 LDCM0411  CL23 HCT 116 C214(0.84); C222(0.84)  LDD0728  [1]
 LDCM0412  CL24 HCT 116 C214(0.85); C222(0.85)  LDD0729  [1]
 LDCM0413  CL25 HCT 116 C214(1.06); C222(1.06)  LDD0730  [1]
 LDCM0414  CL26 HCT 116 C214(0.89); C222(0.89)  LDD0731  [1]
 LDCM0415  CL27 HCT 116 C214(0.98); C222(0.98)  LDD0732  [1]
 LDCM0416  CL28 HCT 116 C214(0.82); C222(0.82)  LDD0733  [1]
 LDCM0417  CL29 HCT 116 C214(0.83); C222(0.83)  LDD0734  [1]
 LDCM0419  CL30 HCT 116 C214(1.11); C222(1.11)  LDD0736  [1]
 LDCM0420  CL31 HCT 116 C214(1.04); C222(1.04)  LDD0737  [1]
 LDCM0436  CL46 HCT 116 C214(1.80); C222(1.80)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C214(1.60); C222(1.60)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C214(1.25); C222(1.25)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C214(1.29); C222(1.29)  LDD0756  [1]
 LDCM0441  CL50 HCT 116 C214(1.12); C222(1.12)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C214(1.52); C222(1.52)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C214(1.13); C222(1.13)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C214(1.11); C222(1.11)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C214(1.43); C222(1.43)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C214(1.45); C222(1.45)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C214(1.43); C222(1.43)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C214(1.58); C222(1.58)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C214(1.28); C222(1.28)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C214(1.43); C222(1.43)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C214(1.39); C222(1.39)  LDD0769  [1]
 LDCM0453  CL61 HCT 116 C214(0.94); C222(0.94)  LDD0770  [1]
 LDCM0454  CL62 HCT 116 C214(0.65); C222(0.65)  LDD0771  [1]
 LDCM0455  CL63 HCT 116 C214(0.70); C222(0.70)  LDD0772  [1]
 LDCM0456  CL64 HCT 116 C214(0.77); C222(0.77)  LDD0773  [1]
 LDCM0457  CL65 HCT 116 C214(0.94); C222(0.94)  LDD0774  [1]
 LDCM0458  CL66 HCT 116 C214(0.91); C222(0.91)  LDD0775  [1]
 LDCM0459  CL67 HCT 116 C214(0.82); C222(0.82)  LDD0776  [1]
 LDCM0460  CL68 HCT 116 C214(1.20); C222(1.20)  LDD0777  [1]
 LDCM0461  CL69 HCT 116 C214(0.84); C222(0.84)  LDD0778  [1]
 LDCM0463  CL70 HCT 116 C214(0.99); C222(0.99)  LDD0780  [1]
 LDCM0464  CL71 HCT 116 C214(0.86); C222(0.86)  LDD0781  [1]
 LDCM0465  CL72 HCT 116 C214(1.19); C222(1.19)  LDD0782  [1]
 LDCM0466  CL73 HCT 116 C214(1.05); C222(1.05)  LDD0783  [1]
 LDCM0467  CL74 HCT 116 C214(0.98); C222(0.98)  LDD0784  [1]
 LDCM0469  CL76 HCT 116 C214(0.87); C222(0.87)  LDD0786  [1]
 LDCM0470  CL77 HCT 116 C214(0.85); C222(0.85)  LDD0787  [1]
 LDCM0471  CL78 HCT 116 C214(0.82); C222(0.82)  LDD0788  [1]
 LDCM0472  CL79 HCT 116 C214(0.90); C222(0.90)  LDD0789  [1]
 LDCM0474  CL80 HCT 116 C214(0.80); C222(0.80)  LDD0791  [1]
 LDCM0475  CL81 HCT 116 C214(0.93); C222(0.93)  LDD0792  [1]
 LDCM0476  CL82 HCT 116 C214(0.96); C222(0.96)  LDD0793  [1]
 LDCM0477  CL83 HCT 116 C214(0.86); C222(0.86)  LDD0794  [1]
 LDCM0478  CL84 HCT 116 C214(0.96); C222(0.96)  LDD0795  [1]
 LDCM0479  CL85 HCT 116 C214(0.83); C222(0.83)  LDD0796  [1]
 LDCM0480  CL86 HCT 116 C214(0.95); C222(0.95)  LDD0797  [1]
 LDCM0481  CL87 HCT 116 C214(1.12); C222(1.12)  LDD0798  [1]
 LDCM0482  CL88 HCT 116 C214(0.93); C222(0.93)  LDD0799  [1]
 LDCM0483  CL89 HCT 116 C214(0.74); C222(0.74)  LDD0800  [1]
 LDCM0485  CL90 HCT 116 C214(1.46); C222(1.46)  LDD0802  [1]
 LDCM0486  CL91 HCT 116 C214(0.75); C222(0.75)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C214(0.85); C222(0.85)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C214(0.73); C222(0.73)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C214(0.74); C222(0.74)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C214(0.87); C222(0.87)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C214(0.99); C222(0.99)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C214(0.84); C222(0.84)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C214(0.88); C222(0.88)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C214(0.82); C222(0.82)  LDD0811  [1]
 LDCM0468  Fragment33 HCT 116 C214(1.02); C222(1.02)  LDD0785  [1]
 LDCM0022  KB02 A101D C268(1.45)  LDD2250  [2]
 LDCM0023  KB03 A101D C268(1.95)  LDD2667  [2]
 LDCM0024  KB05 SK-ES1 C58(1.95); C361(1.83); C268(1.92)  LDD3423  [2]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 DrugMap: A quantitative pan-cancer analysis of cysteine ligandability. Cell. 2024 May 9;187(10):2536-2556.e30. doi: 10.1016/j.cell.2024.03.027. Epub 2024 Apr 22.
Mass spectrometry data entry: PXD047840