Details of the Target

General Information of Target

Target ID LDTP07271
Target Name Terminal nucleotidyltransferase 4A (TENT4A)
Gene Name TENT4A
Gene ID 11044
Synonyms
PAPD7; POLS; TRF4; Terminal nucleotidyltransferase 4A; DNA polymerase sigma; LAK-1; Non-canonical poly(A) RNA polymerase PAPD7; EC 2.7.7.19; PAP-associated domain-containing protein 7; TRAMP-like complex polyadenylate polymerase; Terminal guanylyltransferase; EC 2.7.7.-; Terminal uridylyltransferase 5; TUTase 5; Topoisomerase-related function protein 4-1; TRF4-1
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MDPRVAWIQPEQKGPANALWMQIWETSQGVGRGGSGFASYFCLNSPALDTAAAAGAAGRG
SGGLGPALPAASPPPPGPTAPAALPPALLTALGPAAEGARRLHKSPSLSSSSSSSSSNAE
SGTESPGCSSSSSSSASLGRPGGGRGGAFFNFADGAPSAPGTANGHPGPRGPAPAGSPSQ
HQFHPGRRKRENKASTYGLNYLLSGSRAAALSGGGGPGAQAPRPGTPWKSRAYSPGIQGL
HEEIIDFYNFMSPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDL
VVFGKWERPPLQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETG
VRAAEFIKNYMKKYSLLPYLILVLKQFLLQRDLNEVFTGGISSYSLILMAISFLQLHPRI
DARRADENLGMLLVEFFELYGRNFNYLKTGIRIKEGGAYIAKEEIMKAMTSGYRPSMLCI
EDPLLPGNDVGRSSYGAMQVKQVFDYAYIVLSHAVSPLARSYPNRDAESTLGRIIKVTQE
VIDYRRWIKEKWGSKAHPSPGMDSRIKIKERIATCNGEQTQNREPESPYGQRLTLSLSSP
QLLSSGSSASSVSSLSGSDVDSDTPPCTTPSVYQFSLQAPAPLMAGLPTALPMPSGKPQP
TTSRTLIMTTNNQTRFTIPPPTLGVAPVPCRQAGVEGTASLKAVHHMSSPAIPSASPNPL
SSPHLYHKQHNGMKLSMKGSHGHTQGGGYSSVGSGGVRPPVGNRGHHQYNRTGWRRKKHT
HTRDSLPVSLSR
Target Type
Clinical trial
Target Bioclass
Enzyme
Family
DNA polymerase type-B-like family
Subcellular location
Cytoplasm
Function
Terminal nucleotidyltransferase that catalyzes preferentially the transfer of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation.
TTD ID
T63242
Uniprot ID
Q5XG87
DrugMap ID
TT0XZ4Q
Ensemble ID
ENST00000230859.8
HGNC ID
HGNC:16705
ChEMBL ID
CHEMBL4680033

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 4 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
DBIA
 Probe Info  		C77(0.80)  LDD0531  [1]
NAIA_4
 Probe Info  		N.A.  LDD2226  [2]
TFBX
 Probe Info  		N.A.  LDD0027  [3]
IPM
 Probe Info  		N.A.  LDD0147  [3]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0214  AC1 HCT 116 C77(0.80)  LDD0531  [1]
 LDCM0215  AC10 HCT 116 C77(0.93)  LDD0532  [1]
 LDCM0226  AC11 HCT 116 C77(1.00)  LDD0543  [1]
 LDCM0237  AC12 HCT 116 C77(0.89)  LDD0554  [1]
 LDCM0259  AC14 HCT 116 C77(0.87)  LDD0576  [1]
 LDCM0270  AC15 HCT 116 C77(0.87)  LDD0587  [1]
 LDCM0279  AC2 HCT 116 C77(0.79)  LDD0596  [1]
 LDCM0290  AC3 HCT 116 C77(0.90)  LDD0607  [1]
 LDCM0301  AC4 HCT 116 C77(1.00)  LDD0618  [1]
 LDCM0312  AC5 HCT 116 C77(0.84)  LDD0629  [1]
 LDCM0323  AC6 HCT 116 C77(0.96)  LDD0640  [1]
 LDCM0334  AC7 HCT 116 C77(1.07)  LDD0651  [1]
 LDCM0345  AC8 HCT 116 C77(0.92)  LDD0662  [1]
 LDCM0248  AKOS034007472 HCT 116 C77(1.11)  LDD0565  [1]
 LDCM0356  AKOS034007680 HCT 116 C77(1.03)  LDD0673  [1]
 LDCM0275  AKOS034007705 HCT 116 C77(0.93)  LDD0592  [1]
 LDCM0367  CL1 HEK-293T C307(1.05)  LDD1571  [4]
 LDCM0369  CL100 HCT 116 C77(0.83)  LDD0686  [1]
 LDCM0370  CL101 HCT 116 C77(0.86)  LDD0687  [1]
 LDCM0371  CL102 HCT 116 C77(0.91)  LDD0688  [1]
 LDCM0372  CL103 HCT 116 C77(0.81)  LDD0689  [1]
 LDCM0373  CL104 HCT 116 C77(0.91)  LDD0690  [1]
 LDCM0374  CL105 HEK-293T C307(1.09)  LDD1578  [4]
 LDCM0378  CL109 HEK-293T C307(1.11)  LDD1582  [4]
 LDCM0383  CL113 HEK-293T C307(1.10)  LDD1587  [4]
 LDCM0387  CL117 HEK-293T C307(1.03)  LDD1591  [4]
 LDCM0392  CL121 HEK-293T C307(1.19)  LDD1596  [4]
 LDCM0396  CL125 HEK-293T C307(1.01)  LDD1600  [4]
 LDCM0400  CL13 HEK-293T C307(1.15)  LDD1604  [4]
 LDCM0413  CL25 HEK-293T C307(1.12)  LDD1617  [4]
 LDCM0420  CL31 HCT 116 C77(1.07)  LDD0737  [1]
 LDCM0421  CL32 HCT 116 C77(0.99)  LDD0738  [1]
 LDCM0422  CL33 HCT 116 C77(1.04)  LDD0739  [1]
 LDCM0423  CL34 HCT 116 C77(0.99)  LDD0740  [1]
 LDCM0424  CL35 HCT 116 C77(1.07)  LDD0741  [1]
 LDCM0425  CL36 HCT 116 C77(1.09)  LDD0742  [1]
 LDCM0426  CL37 HCT 116 C77(1.04)  LDD0743  [1]
 LDCM0428  CL39 HCT 116 C77(0.96)  LDD0745  [1]
 LDCM0430  CL40 HCT 116 C77(1.41)  LDD0747  [1]
 LDCM0431  CL41 HCT 116 C77(1.00)  LDD0748  [1]
 LDCM0432  CL42 HCT 116 C77(1.01)  LDD0749  [1]
 LDCM0433  CL43 HCT 116 C77(1.05)  LDD0750  [1]
 LDCM0434  CL44 HCT 116 C77(1.02)  LDD0751  [1]
 LDCM0435  CL45 HCT 116 C77(1.05)  LDD0752  [1]
 LDCM0436  CL46 HCT 116 C77(1.10)  LDD0753  [1]
 LDCM0437  CL47 HCT 116 C77(0.84)  LDD0754  [1]
 LDCM0438  CL48 HCT 116 C77(0.88)  LDD0755  [1]
 LDCM0439  CL49 HCT 116 C77(1.04)  LDD0756  [1]
 LDCM0441  CL50 HCT 116 C77(0.84)  LDD0758  [1]
 LDCM0442  CL51 HCT 116 C77(0.94)  LDD0759  [1]
 LDCM0443  CL52 HCT 116 C77(0.98)  LDD0760  [1]
 LDCM0444  CL53 HCT 116 C77(1.01)  LDD0761  [1]
 LDCM0445  CL54 HCT 116 C77(0.96)  LDD0762  [1]
 LDCM0446  CL55 HCT 116 C77(0.77)  LDD0763  [1]
 LDCM0447  CL56 HCT 116 C77(0.73)  LDD0764  [1]
 LDCM0448  CL57 HCT 116 C77(0.92)  LDD0765  [1]
 LDCM0449  CL58 HCT 116 C77(0.96)  LDD0766  [1]
 LDCM0450  CL59 HCT 116 C77(0.91)  LDD0767  [1]
 LDCM0452  CL60 HCT 116 C77(0.75)  LDD0769  [1]
 LDCM0453  CL61 HEK-293T C307(1.00)  LDD1656  [4]
 LDCM0466  CL73 HEK-293T C307(1.11)  LDD1669  [4]
 LDCM0479  CL85 HEK-293T C307(1.02)  LDD1682  [4]
 LDCM0486  CL91 HCT 116 C77(0.85)  LDD0803  [1]
 LDCM0487  CL92 HCT 116 C77(0.81)  LDD0804  [1]
 LDCM0488  CL93 HCT 116 C77(0.73)  LDD0805  [1]
 LDCM0489  CL94 HCT 116 C77(0.78)  LDD0806  [1]
 LDCM0490  CL95 HCT 116 C77(0.63)  LDD0807  [1]
 LDCM0491  CL96 HCT 116 C77(0.70)  LDD0808  [1]
 LDCM0492  CL97 HCT 116 C77(0.63)  LDD0809  [1]
 LDCM0493  CL98 HCT 116 C77(0.99)  LDD0810  [1]
 LDCM0494  CL99 HCT 116 C77(0.73)  LDD0811  [1]
 LDCM0427  Fragment51 HCT 116 C77(1.69)  LDD0744  [1]

References

1 Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries. Nat Biotechnol. 2021 May;39(5):630-641. doi: 10.1038/s41587-020-00778-3. Epub 2021 Jan 4.
2 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
3 Chemoproteomic Profiling by Cysteine Fluoroalkylation Reveals Myrocin G as an Inhibitor of the Nonhomologous End Joining DNA Repair Pathway. J Am Chem Soc. 2021 Dec 8;143(48):20332-20342. doi: 10.1021/jacs.1c09724. Epub 2021 Nov 24.
Mass spectrometry data entry: PXD029255
4 Accelerating multiplexed profiling of protein-ligand interactions: High-throughput plate-based reactive cysteine profiling with minimal input. Cell Chem Biol. 2024 Mar 21;31(3):565-576.e4. doi: 10.1016/j.chembiol.2023.11.015. Epub 2023 Dec 19.
Mass spectrometry data entry: PXD044402