Details of the Target

General Information of Target

Target ID LDTP06323
Target Name Astrocytic phosphoprotein PEA-15 (PEA15)
Gene Name PEA15
Gene ID 8682
Synonyms
Astrocytic phosphoprotein PEA-15; 15 kDa phosphoprotein enriched in astrocytes; Phosphoprotein enriched in diabetes; PED
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Sequence
MAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFSFLESHNKLDKDNL
SYIEHIFEISRRPDLLTMVVDYRTRVLKISEEDELDTKLTRIPSAKKYKDIIRQPSEEEI
IKLAPPPKKA
Target Bioclass
Other
Subcellular location
Cytoplasm
Function
Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm. Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
Uniprot ID
Q15121
Ensemble ID
ENST00000360472.9
HGNC ID
HGNC:8822

Target Site Mutations in Different Cell Lines

Cell line Mutation details Probe for labeling this protein in this cell
MM1S SNV: p.S70F .
SKMEL24 SNV: p.T6I .

Probe(s) Labeling This Target

ABPP Probe
Click To Hide/Show 11 Probe Related to This Target
Probe name Structure Binding Site(Ratio) Interaction ID Ref
STPyne
 Probe Info  		K107(10.00); K109(5.77); K128(5.05)  LDD0277  [1]
BTD
 Probe Info  		C27(1.47)  LDD1700  [2]
5E-2FA
 Probe Info  		N.A.  LDD2235  [3]
ATP probe
 Probe Info  		N.A.  LDD0199  [4]
m-APA
 Probe Info  		H65(0.00); H52(0.00)  LDD2231  [3]
4-Iodoacetamidophenylacetylene
 Probe Info  		N.A.  LDD0038  [5]
IA-alkyne
 Probe Info  		N.A.  LDD0176  [6]
IPM
 Probe Info  		N.A.  LDD2156  [7]
Acrolein
 Probe Info  		N.A.  LDD0217  [8]
Methacrolein
 Probe Info  		N.A.  LDD0218  [8]
NAIA_5
 Probe Info  		N.A.  LDD2223  [9]

Competitor(s) Related to This Target

Competitor ID Name Cell line Binding Site(Ratio) Interaction ID Ref
 LDCM0548  1-(4-(Benzo[d][1,3]dioxol-5-ylmethyl)piperazin-1-yl)-2-nitroethan-1-one MDA-MB-231 C27(0.47)  LDD2142  [2]
 LDCM0519  1-(6-methoxy-3,4-dihydroquinolin-1(2H)-yl)-2-nitroethan-1-one MDA-MB-231 C27(0.68)  LDD2112  [2]
 LDCM0524  2-Cyano-N-(2-morpholin-4-yl-ethyl)-acetamide MDA-MB-231 C27(0.89)  LDD2117  [2]
 LDCM0558  2-Cyano-N-phenylacetamide MDA-MB-231 C27(1.44)  LDD2152  [2]
 LDCM0539  3-(4-Isopropylpiperazin-1-yl)-3-oxopropanenitrile MDA-MB-231 C27(0.53)  LDD2132  [2]
 LDCM0545  Acetamide MDA-MB-231 C27(0.23)  LDD2138  [2]
 LDCM0520  AKOS000195272 MDA-MB-231 C27(0.59)  LDD2113  [2]
 LDCM0498  BS-3668 MDA-MB-231 C27(0.43)  LDD2091  [2]
 LDCM0108  Chloroacetamide HeLa N.A.  LDD0222  [8]
 LDCM0625  F8 Ramos C27(1.68)  LDD2187  [10]
 LDCM0572  Fragment10 Ramos C27(1.76)  LDD2189  [10]
 LDCM0573  Fragment11 Ramos C27(0.15)  LDD2190  [10]
 LDCM0574  Fragment12 Ramos C27(2.26)  LDD2191  [10]
 LDCM0575  Fragment13 Ramos C27(3.21)  LDD2192  [10]
 LDCM0576  Fragment14 Ramos C27(0.90)  LDD2193  [10]
 LDCM0579  Fragment20 Ramos C27(1.50)  LDD2194  [10]
 LDCM0580  Fragment21 Ramos C27(1.41)  LDD2195  [10]
 LDCM0582  Fragment23 Ramos C27(2.48)  LDD2196  [10]
 LDCM0586  Fragment28 Ramos C27(0.72)  LDD2198  [10]
 LDCM0588  Fragment30 Ramos C27(2.44)  LDD2199  [10]
 LDCM0590  Fragment32 Ramos C27(2.36)  LDD2201  [10]
 LDCM0468  Fragment33 Ramos C27(3.05)  LDD2202  [10]
 LDCM0596  Fragment38 Ramos C27(1.63)  LDD2203  [10]
 LDCM0566  Fragment4 Ramos C27(0.68)  LDD2184  [10]
 LDCM0614  Fragment56 Ramos C27(4.67)  LDD2205  [10]
 LDCM0569  Fragment7 Ramos C27(0.75)  LDD2186  [10]
 LDCM0571  Fragment9 Ramos C27(1.81)  LDD2188  [10]
 LDCM0107  IAA HeLa N.A.  LDD0221  [8]
 LDCM0022  KB02 Ramos C27(1.10)  LDD2182  [10]
 LDCM0023  KB03 Ramos C27(0.69)  LDD2183  [10]
 LDCM0024  KB05 Ramos C27(0.64)  LDD2185  [10]
 LDCM0528  N-(4-bromophenyl)-2-cyano-N-phenylacetamide MDA-MB-231 C27(0.35)  LDD2121  [2]
 LDCM0496  Nucleophilic fragment 11a MDA-MB-231 C27(0.36)  LDD2089  [2]
 LDCM0499  Nucleophilic fragment 12b MDA-MB-231 C27(1.03)  LDD2092  [2]
 LDCM0500  Nucleophilic fragment 13a MDA-MB-231 C27(0.70)  LDD2093  [2]
 LDCM0501  Nucleophilic fragment 13b MDA-MB-231 C27(1.91)  LDD2094  [2]
 LDCM0503  Nucleophilic fragment 14b MDA-MB-231 C27(0.65)  LDD2096  [2]
 LDCM0504  Nucleophilic fragment 15a MDA-MB-231 C27(0.80)  LDD2097  [2]
 LDCM0505  Nucleophilic fragment 15b MDA-MB-231 C27(0.75)  LDD2098  [2]
 LDCM0506  Nucleophilic fragment 16a MDA-MB-231 C27(0.91)  LDD2099  [2]
 LDCM0507  Nucleophilic fragment 16b MDA-MB-231 C27(0.41)  LDD2100  [2]
 LDCM0508  Nucleophilic fragment 17a MDA-MB-231 C27(0.65)  LDD2101  [2]
 LDCM0511  Nucleophilic fragment 18b MDA-MB-231 C27(0.45)  LDD2104  [2]
 LDCM0512  Nucleophilic fragment 19a MDA-MB-231 C27(0.98)  LDD2105  [2]
 LDCM0514  Nucleophilic fragment 20a MDA-MB-231 C27(0.93)  LDD2107  [2]
 LDCM0515  Nucleophilic fragment 20b MDA-MB-231 C27(0.48)  LDD2108  [2]
 LDCM0516  Nucleophilic fragment 21a MDA-MB-231 C27(0.61)  LDD2109  [2]
 LDCM0518  Nucleophilic fragment 22a MDA-MB-231 C27(0.99)  LDD2111  [2]
 LDCM0521  Nucleophilic fragment 23b MDA-MB-231 C27(0.84)  LDD2114  [2]
 LDCM0522  Nucleophilic fragment 24a MDA-MB-231 C27(0.39)  LDD2115  [2]
 LDCM0526  Nucleophilic fragment 26a MDA-MB-231 C27(3.17)  LDD2119  [2]
 LDCM0527  Nucleophilic fragment 26b MDA-MB-231 C27(0.93)  LDD2120  [2]
 LDCM0530  Nucleophilic fragment 28a MDA-MB-231 C27(0.65)  LDD2123  [2]
 LDCM0532  Nucleophilic fragment 29a MDA-MB-231 C27(0.60)  LDD2125  [2]
 LDCM0534  Nucleophilic fragment 30a MDA-MB-231 C27(0.87)  LDD2127  [2]
 LDCM0535  Nucleophilic fragment 30b MDA-MB-231 C27(0.91)  LDD2128  [2]
 LDCM0536  Nucleophilic fragment 31 MDA-MB-231 C27(0.97)  LDD2129  [2]
 LDCM0540  Nucleophilic fragment 35 MDA-MB-231 C27(0.39)  LDD2133  [2]
 LDCM0541  Nucleophilic fragment 36 MDA-MB-231 C27(0.40)  LDD2134  [2]
 LDCM0542  Nucleophilic fragment 37 MDA-MB-231 C27(0.75)  LDD2135  [2]
 LDCM0543  Nucleophilic fragment 38 MDA-MB-231 C27(1.04)  LDD2136  [2]
 LDCM0544  Nucleophilic fragment 39 MDA-MB-231 C27(0.90)  LDD2137  [2]
 LDCM0211  Nucleophilic fragment 3b MDA-MB-231 C27(1.47)  LDD1700  [2]
 LDCM0546  Nucleophilic fragment 40 MDA-MB-231 C27(0.53)  LDD2140  [2]
 LDCM0547  Nucleophilic fragment 41 MDA-MB-231 C27(0.70)  LDD2141  [2]
 LDCM0549  Nucleophilic fragment 43 MDA-MB-231 C27(1.06)  LDD2143  [2]
 LDCM0552  Nucleophilic fragment 6a MDA-MB-231 C27(0.75)  LDD2146  [2]
 LDCM0553  Nucleophilic fragment 6b MDA-MB-231 C27(4.50)  LDD2147  [2]
 LDCM0554  Nucleophilic fragment 7a MDA-MB-231 C27(0.43)  LDD2148  [2]
 LDCM0556  Nucleophilic fragment 8a MDA-MB-231 C27(0.30)  LDD2150  [2]
 LDCM0557  Nucleophilic fragment 8b MDA-MB-231 C27(0.60)  LDD2151  [2]

The Interaction Atlas With This Target

The Protein(s) Related To This Target

Enzyme
Click To Hide/Show 2 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Mitogen-activated protein kinase 1 (MAPK1) CMGC Ser/Thr protein kinase family P28482
Mitogen-activated protein kinase 3 (MAPK3) CMGC Ser/Thr protein kinase family P27361
Transcription factor
Click To Hide/Show 1 Protein(s) Interacting with This Target
Protein name Family Uniprot ID
Telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP) RAP1 family Q9NYB0

References

1 A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells. Chem Sci. 2021 Oct 15;12(43):14557-14563. doi: 10.1039/d1sc02230j. eCollection 2021 Nov 10.
Mass spectrometry data entry: PXD028270
2 Nucleophilic covalent ligand discovery for the cysteine redoxome. Nat Chem Biol. 2023 Nov;19(11):1309-1319. doi: 10.1038/s41589-023-01330-5. Epub 2023 May 29.
Mass spectrometry data entry: PXD039908 , PXD029761
3 Global profiling of functional histidines in live cells using small-molecule photosensitizer and chemical probe relay labelling. Nat Chem. 2024 Jun 4. doi: 10.1038/s41557-024-01545-6. Online ahead of print.
Mass spectrometry data entry: PXD042377
4 Targeted Proteomic Approaches for Proteome-Wide Characterizations of the AMP-Binding Capacities of Kinases. J Proteome Res. 2022 Aug 5;21(8):2063-2070. doi: 10.1021/acs.jproteome.2c00225. Epub 2022 Jul 12.
5 Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation. Anal Chem. 2022 Mar 8;94(9):3800-3810. doi: 10.1021/acs.analchem.1c04402. Epub 2022 Feb 23.
Mass spectrometry data entry: PXD028853
6 SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*. Chembiochem. 2021 May 14;22(10):1841-1851. doi: 10.1002/cbic.202000870. Epub 2021 Feb 18.
Mass spectrometry data entry: PXD023056 , PXD023059 , PXD023058 , PXD023057 , PXD023060
7 Benchmarking Cleavable Biotin Tags for Peptide-Centric Chemoproteomics. J Proteome Res. 2022 May 6;21(5):1349-1358. doi: 10.1021/acs.jproteome.2c00174. Epub 2022 Apr 25.
Mass spectrometry data entry: PXD031019
8 ACR-Based Probe for the Quantitative Profiling of Histidine Reactivity in the Human Proteome. J Am Chem Soc. 2023 Mar 8;145(9):5252-5260. doi: 10.1021/jacs.2c12653. Epub 2023 Feb 27.
9 N-Acryloylindole-alkyne (NAIA) enables imaging and profiling new ligandable cysteines and oxidized thiols by chemoproteomics. Nat Commun. 2023 Jun 15;14(1):3564. doi: 10.1038/s41467-023-39268-w.
Mass spectrometry data entry: PXD041264
10 Site-specific quantitative cysteine profiling with data-independent acquisition-based mass spectrometry. Methods Enzymol. 2023;679:295-322. doi: 10.1016/bs.mie.2022.07.037. Epub 2022 Sep 7.
Mass spectrometry data entry: PXD027578